Objective To investigate the repairative effect of local high expression of glial cell line-derived neurotrophic factor (GDNF) in the repair of sciatic nerve defects. Green fluorescent protein (GFP) gene was used as reporter gene. Two groups including gel matrix control group and gel matrix plus MSCs group were set ( 10 rabbits per group). Methods GDNF secreting mesenchymal stem cells (MSCs) mediated by lentiviral vectors were suspended in Matrigel to produce the artificial graft, and then it was locally implanted to a 20 mm-long sciatic nerve defect in rabbits. Results In the GDNF group,the nerve continuity was restored in seven rabbits and the functional amelioration of the wounded limb was apparent. While there found no restoration of the nerve continuity in the control groups. The migration distance of GDNF secreting MSCs was 20 mm, suggesting a good integration of the transplanted cells with host cells. The glial fibrillary acidic protein (GFAP) + green fluorescent protein (GFP) and -100 protein GFP accounted for 60% and 70% respectively in the experimental group, while it was only 40% and 30% in gel matrix plus MSCs group. Conclusions Local implantation of GDNF secreting MSCs can promote the regeneration and functional recovery of the sciatic nerves. GDNF seems to play an important role in inducing transdifferentiation of MSCs to Schwann cell lineage.

Ischemia-reperfusion injury is a pathophysiological process caused by a number of gene expression changes,with the development of artery occlusion-reperfusion and surgery involved in vascular,such as thrombolytic therapy,cardiopulmonary bypass heart surgery and organ transplants,etc.it has been more and more important.Gene chip technology provides a powerful means for the systematic study of the mechanisms of incidence and prevention of ischemia-reperfusion injury,for its unique advantage in high throughput,high-efficiency,large-scale research.Since gaining importance in recent years,it has been widely used in various organs and tissues,and has achieved some progress.In this paper,the application of gene chip technology in the incidence and prevention of ischemia-reperfusion injury of various organs and tissues in recent years is given a certain summary.

Objective To compare the stray-light effects caused by different parts of optic edge of 7 intraocular lens(IOL)edge designs.Methods Monochromatic laser beam was used to illuminate the upper edge and complex of three-piece and single-piece IOLs at several angles of incidence.Light images produced in the retinal plane were photographed with a digital camera.The differences in the light images were compared between three-piece and single-piece IOLs.Results There were differences in light images between 2 groups,which may be due to different shapes of edge design when the upper edge of IOL was illuminated.Three-piece IOL exhibited line images or dense patch,which differed with single-piece IOL when the complex of IOL was illuminated.Conclusion Both edge shape and irregular structure in three-piece IOL complex may be important factors for postoperative glare in pseudophakic patients.

Objective To investigate the indoor air formaldehyde pollution in the human anatomy laboratory and to study the control measures and their effects on prevention and control of formaldehyde pollution. Methods The levels of formaldehyde in the air of the human anatomy laboratory with exhaust ventilatary conditional system and without such a system were determined respectively according to the method recommended in the Sanitary Standard of Public Place. Results Without the exhaust ventilatory conditional system, the level of formaldehyde of indoor air reached to 8.349 mg/m3, while the level of formaldehyde was decreased significantly to 0.501 mg/m3 with the system. Conclusion Without the exhaust ventilatory conditional system, the formaldehyde pollution in the air of the human anatomy laboratory was very serious, which might have adverse effects on teachers' and students' health, while with the system, the formaldehyde pollution could be controlled effectively.

Six five renal graft biopsies were performed routinely in 36 renal transplantation patients with normal renal function 1 and 3 months after transplantation. All the patients were given the immunosuppressive regimen of cyclosporine A (CsA), prednisone (Pred), azathioprine (Aza) and Tripterygium Wilfordii Hook (TW). The histological diagnosis of allograft biopsy was made according to the Banff schema. In 18 45% of the biopsies (12/65), early subclinical rejection or borderline changes were found. There was a significant difference for different dosages of TW, but no difference for the dosages of CsA, Pred and Aza intravenous between patients with pathological changes and those without. The patients with early subclinical rejection or borderline changes, methylpredisolone was given in bolus, followed by the adjustment of immunosuppressive regimens. With this treatment, there was no difference in 5 year survival rate between them and those with normal renal allograft biopsy findings. The routine allograft biopsy helps discover early subclinical rejection and borderline changes in patients with renal transplantation, and beneficial for adjustment of immunosuppressive therapy.

Objective To investigate the effects of inhibition of nuclear factor-kappa B (NF-?B) by pyrrolidine dithiocarbamate (PDTC) on tissue pro- and anti-inflammatory cytokine expression in rats with postburn Staphylococcus aureus sepsis and its potential regulating mechanism. Methods Thirty-four male Wistar rats were randomly divided into normal control group (n=6), scald control group (n=6), postburn sepsis group (n=12),and PDTC treatment group (n=10). Tissue samples from the liver, kidneys and lungs were collected to determine tumor necrosis factor-? (TNF-?) and interleukin-10 (IL-10) mRNA expression as well as their protein levels. Results Both TNF-? mRNA and protein expressions in the liver, kidneys and lungs of postburn septic animals were up-regulated rapidly at 0.5-2 hours (P

Theprospectofliverdiseasetreatmentbyhepatocytetransplantationisbroad ,butitis difficulttoapplyitinclinictherapyduetotherestrictionofsourceandproliferationofdonorhepatocyte .The hematopoieticstemcellplasticityoftrans -differentiationtohepatocyteprovidesanewsourceofseedcellsfor hepatocytetransplantation .Inthisreview ,wefocusonadvancesintheseedcellsforhepatocytetransplanta tion .

OBJECTIVE:To establish a method for the content determination of glabridin in Clycyrrhizae Radix et Rhizoma from different regions. METHODS:HPLC was performed on the column of Inertsil ODS-SP with mobile phase of acetoni-trile-0.05%phosphoric acid(gradient elution)at a flow rate of 0.8 ml/min,detection wavelength was 280 nm,the column tempera-ture was 35 ℃,and the injection volume was 20 μl. RESULTS:The linear range of glabridin was 0.906-18.12 μg/ml(r=0.999 7), RSDs of precision,stability and reproducibility tests were lower than 3%;recovery was 98.73%-101.90%(RSD=1.25%,n=6). There were obvious differences among the glabridin contents in G. uralensis from different regions. CONCLUSIONS:The method is simple and accurate with high precision,and can be used for the content determination of glabridin in Clycyrrhizae Radix et Rhi-zoma.

Objective To observe the preliminary effect and safety of perioperative hyperthermic intraperitonral chemotherapy to treat gastrointestinal cancer. Methods The clinical data of 60 patients with progressed gastric or colorectal carcinoma who underwent perioperatively HIPEC in our hospital from May, 2012 to October, 2013 were retrospectively analyzed. The incision healing, complications, KPS scores and serum CEA levels were observed. Results The vital signs of all patients were normal during HIPEC. There was no perioperative death. No patients underwent serious complications like diffuse peritonitis, intestinal obstruction, gastrointestinal perforation or intraperitoneal bleeding. There was no anastomotic leakage in 28 patients who underwent Stomach-jejunum anastomosis or intestinal anastomosis. After HIPEC, the life quality was improved;increase in KPS score and reduction in serum CEA levels were noted in all patients (P < 0.01). Of 29 patients with malignant ascites, 20 cases achieved complete mitigation and 8 cases achieved partial mitigation, 1 case was in stable condition, thus yielding effective rate of 96.5%. Conclusions It is safe and feasible for HIPEC to treat gastrointestinal cancer. HIPEC can improve the patients’ life qualities, without theincrease in perioperative complications. The short-term effect of HIPEC is confirmed in alleviating ascites.