Ischemia-reperfusion injury is a pathophysiological process caused by a number of gene expression changes,with the development of artery occlusion-reperfusion and surgery involved in vascular,such as thrombolytic therapy,cardiopulmonary bypass heart surgery and organ transplants,etc.it has been more and more important.Gene chip technology provides a powerful means for the systematic study of the mechanisms of incidence and prevention of ischemia-reperfusion injury,for its unique advantage in high throughput,high-efficiency,large-scale research.Since gaining importance in recent years,it has been widely used in various organs and tissues,and has achieved some progress.In this paper,the application of gene chip technology in the incidence and prevention of ischemia-reperfusion injury of various organs and tissues in recent years is given a certain summary.

Objective To investigate the repairative effect of local high expression of glial cell line-derived neurotrophic factor (GDNF) in the repair of sciatic nerve defects. Green fluorescent protein (GFP) gene was used as reporter gene. Two groups including gel matrix control group and gel matrix plus MSCs group were set ( 10 rabbits per group). Methods GDNF secreting mesenchymal stem cells (MSCs) mediated by lentiviral vectors were suspended in Matrigel to produce the artificial graft, and then it was locally implanted to a 20 mm-long sciatic nerve defect in rabbits. Results In the GDNF group,the nerve continuity was restored in seven rabbits and the functional amelioration of the wounded limb was apparent. While there found no restoration of the nerve continuity in the control groups. The migration distance of GDNF secreting MSCs was 20 mm, suggesting a good integration of the transplanted cells with host cells. The glial fibrillary acidic protein (GFAP) + green fluorescent protein (GFP) and -100 protein GFP accounted for 60% and 70% respectively in the experimental group, while it was only 40% and 30% in gel matrix plus MSCs group. Conclusions Local implantation of GDNF secreting MSCs can promote the regeneration and functional recovery of the sciatic nerves. GDNF seems to play an important role in inducing transdifferentiation of MSCs to Schwann cell lineage.

0.05).The pregnancy rate was 41.5%(17/41) in the(Non-cystectomy) Group,33.3%(23/69) in the Laparoscopic Group,and 25.5%(14/55) in the Open Group,respectively,without significant differences(?~2=2.754,P=0.252).A total of 86 patients(90 cycles) were given a unilateral cystectomy,including 37 cycles in the Open Group and 53 cycles in the Laparoscopic Group.In the 37 cycles of the Open Group,the number of dominant follicles was less in affected ovary(4.41?4.02) than in contralateral one(6.14?4.37)(t=-2.364,P=0.024),whereas in the 53 cycles of the Laparoscopic Group,the number of dominant follicles was significantly less in affected ovary(3.33?3.50) than in contralateral ovary(6.40?3.61)(t=-5.358,P=0.000).Conclusions Both laparoscopic and open cystectomy of ovarian endometrioma may cause damage to ovarian response of COH.

Objective To study the correlation between TSH decrease and post‐stroke depression .Methods 101 patients with acute brain infarction in our hospital from October 2012 to July 2014 were collected as the study subjects and divided into the TSH decrease group and the TSH normal group according to whether serum TSH levels less than 0 .27 uIU/mL .The two groups were evaluated on admission by the National Institute of Health Stroke Scale (NIHSS) ,at 3 months after discharge by modifies Rankin′s Scale(mRS)and the Hamilton Depression Scale(HAMD)respectively .Results The HAMD scores at 3 months in the TSH decrease group were significantly higher than those in the TSH normal group (16 .04 ± 3 .34 vs .14 .03 ± 3 .47 ,P=0 .000)and the mRS scores NIHSS score were significantly higher than those in the TSH normal (3 .2 ± 1 .1 vs .2 .1 ± 1 .5 ,P<0 .05)) .The ra‐tio of poor prognosis in the TSH decrease group was higher than that in the TSH normal group (89 .3% vs .38 .4% ,P<0 .05) ,in‐dicating that the prognosis in the TSH decrease group was poor .Conclusion The correlation could exist between the decrease of TSH level with post‐stroke depression .In acute brain infarction ,the patient with low TSH level could have the poor prognosis .

Objective To investigate the indoor air formaldehyde pollution in the human anatomy laboratory and to study the control measures and their effects on prevention and control of formaldehyde pollution. Methods The levels of formaldehyde in the air of the human anatomy laboratory with exhaust ventilatary conditional system and without such a system were determined respectively according to the method recommended in the Sanitary Standard of Public Place. Results Without the exhaust ventilatory conditional system, the level of formaldehyde of indoor air reached to 8.349 mg/m3, while the level of formaldehyde was decreased significantly to 0.501 mg/m3 with the system. Conclusion Without the exhaust ventilatory conditional system, the formaldehyde pollution in the air of the human anatomy laboratory was very serious, which might have adverse effects on teachers' and students' health, while with the system, the formaldehyde pollution could be controlled effectively.

Objective To investigate the effects of inhibition of nuclear factor-kappa B (NF-?B) by pyrrolidine dithiocarbamate (PDTC) on tissue pro- and anti-inflammatory cytokine expression in rats with postburn Staphylococcus aureus sepsis and its potential regulating mechanism. Methods Thirty-four male Wistar rats were randomly divided into normal control group (n=6), scald control group (n=6), postburn sepsis group (n=12),and PDTC treatment group (n=10). Tissue samples from the liver, kidneys and lungs were collected to determine tumor necrosis factor-? (TNF-?) and interleukin-10 (IL-10) mRNA expression as well as their protein levels. Results Both TNF-? mRNA and protein expressions in the liver, kidneys and lungs of postburn septic animals were up-regulated rapidly at 0.5-2 hours (P

Objective To investigate the effects of advanced glycation endproducts (AGEs) on proliferation of pericytes of bovine retinal capillary vessels and expression of transforming growth factor ?(TGF-?). Methods [WTBZ]The proliferation of pericytes detected by methyl thiazolyl tetrazolium (MTT) colorimetric assay, cellular cycle of pericytes was analyzed by flow cytometry was used to analyze cell, and TGF-? protein expression of pericytes was observed by immunofluorescent staining. Results AGEs inhibited the proliferation of pericytes of bovine retinal capillary vessels, stopped the cellular cycle of pericytes in synthesis phase (S phase), increased the number of apoptotic cells obviously (P

Theprospectofliverdiseasetreatmentbyhepatocytetransplantationisbroad ,butitis difficulttoapplyitinclinictherapyduetotherestrictionofsourceandproliferationofdonorhepatocyte .The hematopoieticstemcellplasticityoftrans -differentiationtohepatocyteprovidesanewsourceofseedcellsfor hepatocytetransplantation .Inthisreview ,wefocusonadvancesintheseedcellsforhepatocytetransplanta tion .

Objective To compare the stray-light effects caused by different parts of optic edge of 7 intraocular lens(IOL)edge designs.Methods Monochromatic laser beam was used to illuminate the upper edge and complex of three-piece and single-piece IOLs at several angles of incidence.Light images produced in the retinal plane were photographed with a digital camera.The differences in the light images were compared between three-piece and single-piece IOLs.Results There were differences in light images between 2 groups,which may be due to different shapes of edge design when the upper edge of IOL was illuminated.Three-piece IOL exhibited line images or dense patch,which differed with single-piece IOL when the complex of IOL was illuminated.Conclusion Both edge shape and irregular structure in three-piece IOL complex may be important factors for postoperative glare in pseudophakic patients.