High mobility group box-B1 (HMGB1)is a non histone DNA-binding protein and extracellular molecule related to damage.Normally it is located inside the cell and exerts a variety of biological effects. It has been demonstrated that the expression of HMGB1 presents in almost all gastrointestinal tumors and is closely correlated with the cell growth, proliferation, invasion and metastasis of gastrointestinal tumors. It has been discovered that HMGB1 inhibitor inhibits growth and metastasis of gastrointestinal tumors, which might become a new approach to the treatment of gastrointestinal tumors.

Objective To evaluate the immune reconstruction of patients with advanced gastric cancer treated by autologous peripheral blood stem cells following chemotherapy. Methods 54 patients with advanced stage of gastric cancer were accepted arterial chemotherapy under the guidance of DSA with the regimen of high dosage of EAP (VP16 100 mg/m 2, topmycine60 mg/m 2 and carplatine200 mg/m 2). Among them 20 patients underwent autologous peripheral blood stem cell transplantation after 48 hours of the chemothrapy. Results Effective rate of EAP arterial chemotherapy combined with autologous peripheral blood stem cell transplantation was up to 85.0% (17/20) in advanced gastric cancer, 29.3% (10/34) in control group ( P

Objective To study the effect of an novel angiogenesis inhibitor, NM-3, on primary tumor growth of human gastric cancer in vivo, and to examine apoptosis of gastric cancer cells induced by NM-3. Methods We established xenotransplanted model of a human gastric cancer cell SGC-7901 in the dorsal skin of nude mice. NM-3 was injected peritoneally at dose of 10 mg/kg, 20 mg/kg or 40 mg/kg every other day for 5 weeks, combined with carboplatin (5 mg/kg) every third day for 4 weeks. As controls of combined treatment, the another two groups of mice were injected with NM-3 or carboplatin alone. Seven weeks after implantation, tumor size, tumor growth inhibition rate, and apoptotic index (AI) were evaluated respectively after sacrifice of the mice. Results Compared with the untreated controls, growth of the tumor implanted subcutaneously was significantly reduced in size in the mice treated with NM-3 10 mg/kg, 20 mg/kg, or 40 mg/kg , with an inhibition rate of 23.0%,40.6% and 56.2%, respectively. The AI increased significantly in the mice treated with NM-3 combined with carboplatin (1.37%?0.19% versus 6.96%?0.65%, 10.65%?1.43%, and 21.66%?2.96% ). Conclusions NM-3, as an angiogenesis inhibitor, can induce apoptosis in gastric cancer and has strong inhibitory effect on tumor growth of human gastric cancer implanted in nude mice.

Objective To study the relationship between contents of duodenogastric reflux and Helicobacter pylori (H.pylori) infection in patients with peptic ulcer. Methods Seventy patients with peptic ulcer diagnosed by endoscopy entered the study. Contents of duodenogastric reflux was detected by 99M TC- EHIDA and H.pylori status was determined by both histology with Giemsa staining and serum anti-H.pylori-IgG level using ELISA method. Results In patients without contents of duodenogastric reflux, H.pylori infection rate was 83.3%(35/42), significant higher than 39.3%(11/28) in those with contents of duodenogastric reflux (P

Objective:To study the influence of matrine on serum levels of IL 6,IL 8,IL 10 in patients with liver fibrosis.Methods:60 patients with liver fibrosis were divided into two groups,hepatic function of 34 patients was aggravated and 26 cases were restored or near to normal levels after oral matrine therapy for three months.serum levels of IL 6,IL 8,IL 10 were measured by ELISA methods in all these patients before and after therapy and 60 healthy subjects.Results:The serum levels of IL 6,IL 8,IL 10 of patients with liver fibrosis were significantly higher than healthy subjects.The serum levels of IL 6,IL 8 in aggravated hepatic function group after therapy were significantly higher than the levels before the therapy,the serum levels of IL 10 was higher than the level before therapy,but there was no significant difference between them.and there was no significant difference of serum levels of IL 6,IL 8 in restored normal hepatic function group after therapy,but the serum level of IL 10 was significantly higher than the level before therapy in this group.Conclusion:Serum levels of IL 6,IL 8,IL 10 may be correlated with the prognosis of patients with liver fibrosis.They may be taken as one of markers to judge the prognosis of patients with liver fibrosis,matrine may have an important inhibitive role on liver fibrosis.

Objective To evaluate the inhibitory effect of 2-(8-hydroxy-6-methoxy-1-oxo-1-H-2-benzopyran-3-Y1) propionic acid (NM-3) on lymphangiogenesis in gastric cancer using orthotopic implantated tumor models of BALB/C nude mice. Methods A BALB/C nude mouse model of transplanted in situ human gastric cancer was established. Twenty-eight nude mice were divided into four groups with 7 each: control group, NM-3 treated group, carboplatin (10 mg/kg) treated group,and NM-3 combiantion group injected with normal saline, 5 mg/kg of NM-3, 10 mg/kg of carboplatin or 5 mg/kg of NM-3, + 10 mg/kg carboplatin, respectively, twice a week for 8 weeks. At the end of the 8th week, all mice were sacrificed for detection of lymphatic microvessel density (LMVD),lymphatic vessel endothelial hyaluranic acid receptor 1 (LYVE-1), podoplanin and Prox-1 byimmunohistochemistry with staining. Results In comparison with control group, the LYVE-1 level in other three groups was decreased with no significant difference (P＞ 0.05). The concentrations of podoplanin and Prox-1 in NM-3 group and combination group decreased significantly than those in control group and carboplatin group (P ＜ 0.05). The number of LMVD in NM-3 group and combination group was 4.72±0.50 and 4.78± 0.38, respectively, which was significantly lower than that in control group (7.35±0.55)and carboplatin group (6.98i0.35, P＜0.05). Conclusion The NM-3 can inhibit the growth of gastric cancer by interfering lymphangiogenesis of gastric cancer.

Objective To investigate the expression changes of nuclear factor(NF)-κB and activator protein (AP)-1 in oxazolone induced colitis in mice and their mechanisms. Methods Twenty-four mice were randomly divided into normal group and model group with 12 each. Experimental colitis was induced with skin sensitization of 3% oxazolone for 5 days, then rectal administration of 0.15 ml of 0. 5% oxazolone solution in mice. All mice were sacrificed on day 3. Peripheral blood mononuclear cells (PBMC), spleen mononuclear cells (SMC) and lamina propria mononuclear cells (LPMC) were isolated from the colon tissues. Expression of NF-κB and AP-1 in SMC, LPMC and PBMC were determined by fluorescence quantitative polymerase chain reaction (PCR). The colitis was evaluated histologically. Results The expressions of NF-κB and AP-1 in SMC, LPMC,PBMC of model groupwere significantly higher than those in normal group(NF-κB : 5.62±0.78 vs. 3.16±0.59,5.46±0.38 vs. 3.18±0.58, 5.65±0.56 vs. 3.36±0.59, P＜0.01; AP-1; 5.61±0.54 vs. 3.22±0.50, 5.50±0.69 vs. 3.19± 0.40,5.67±0.44 vs. 3. 27±0.41, P＜0.01). Conclusion The activation of NF-κB and AP-1 are involved in the mechanisms of ulcerative colitis.

Objective: To study the effects of supplemented Yang Supplementing Five Returning Decoction on the blood lipid and immune function of the model mouse. Methods: Supplemented Yang Supplementing Five Returning Decoction is composed of Yang Supplementing Five Returning Decoction, Herba Epimedii and propolis. The effects of two formulae, Yang Supplementing Five Returning Decoction and Supplemented Yang Supplementing Five Returning Decoction on the blood lipid, the functions of nonspecific immunity, humoral immunity and cellular immunity were investigated on the normal and hyperlipemia rat models and immunosuppressive mouse model. Results: Supplemented Yang Supplementing Five Returning Decoction could not only maintain the original formula functions of lowering TC. TG and LDL C of rats ( P

Objective · To investigate the effects of simo decoction combined with mannitol on the intestinal preparation of magnetically guided capsule endoscopy (MGCE). Methods · A total of 120 patients were enrolled in this randomized controlled trial and randomly divided into two groups according to the random number table. The observation group (n=60) was pretreated with simo decoction 20 mL each time, 3 times a day combined with mannitol 500 mL for oral administration before MGCE examination, while control group (n=60) only was done with mannitol 500 mL for oral administration. Then the intestinal cleanliness and occurrence of adverse reaction were observed. Results · The intestine bubbles in observation group were less than that of control group based on evaluation criteria (Z=-1.976, P=0.048), and the intestine juices in observation group were also less than control group (Z=-2.489,P=0.013) suggesting that the intestinal cleanliness in observation group was superior to control group. Simultaneously, the score of nausea and vomiting in observation group was lower than control group according to the evaluation criteria (Z=-2.215, P=0.027), and abdominal pain, distension and anal stenosis in observation group were less than control group (Z=-2.158, P=0.031), meanwhile, the overall incidences of adverse reaction were 8.33% and 36.67%, respectively (χ2=13.811, P=0.000) , all of which implied that the occurrence of adverse reaction was lower in observation group compared to control group. Conclusion · Simo decoction combined with mannitol could enhance intestinal cleanliness and reduce the occurrence of adverse reaction so as to improve the effect of intestinal preparation.

To investigate the effects of oxymatrine injection (OI) combined with low-dose paclitaxel on expressions of mRNAs and proteins of vascular endothelial growth factor (VEGF) and CXC chemokine receptor 4 (CXCR4) in human gastric carcinoma SGC-7901 cells.