Objective:To observe effects of Panax pseudo-ginseng saponins on protein expression of VEGF, bFGF in myocardium in acute myocardial infarction rats. Methods: The acute myocardial infraction (AMI) model was established in one hundred and forty Wistar rats, and the rats were randomly divided into five groups: the western medicine group mobilized by subcutaneous injection of G-CSF50?g.kg-1.d-1, sham-operated group and model group treated by subcutaneous injection of normal saline 50?g.kg-1.d-1, the Chinese medicine group mobilized by intraperitoneal injection of Xuesaitong (ingredients of Panax pseudo-ginseng saponins) 150mg.kg-1.d-1, the integrative group mobilized by subcutaneous injection of G-CSF 50?g.kg-1.d and intraperitoneal injection of Xuesaitong 150mg.kg-1.d-1. Except for the sham-operated group, each group was divided into three sub-groups by three time points of 1d, 7d and 14d. G-CSF was used once a day for 7 days. Xuesaitong was injected once a day until the rats were killed. The parameters cardiac function in rats with myocardial infarction were detection by color doppler ultrasonic diagnostic apparatus in different times,the expression of VEGF, bFGF in Marginal zone of myocardium in rats with myocardial infarction were detected by immunohistochemistry in different time. Pathological and ultrastructural changes of marginal zone in rats with acute myocardial infarction were observed by electron microscopy and light microscopy. Results:Panax pseudo-ginseng saponins can improve the level of left ventricular systolic function such as EF,FS,it can inhibit the increase of left ventricular LVDD and LVDS,it can improve expression levels of VEGF,bFGF of marginal zone in acute myocardial infarction rats. It also can reduce the damage to cell ultrastructure and promote revascularization in the site around MI area. Panax pseudo-ginseng saponins can protect the myocytes in rats with myocardial infarction. Conclusion:Panax pseudo-ginseng saponins can protect myocardium from ischemic injury in rats after AMI by way of improving expressions of VEGF and bFGF in myocardial cells and promoting angiogenesis in the infarcted of myocardium.

Objective To investigate the correlations between chlamydia pneumoniae (Cpn) infection and acute myocardial infarction(AMI). Methods Specific antibodies and DNA to Cpn were measured in 51 patients with AMI(AMI group),42 patients with elderly myocardial infarction (OMI group) and 31 normal controls (NC group), 33 patients(UC group)who had no angiographic evidence of coronary artery disease. Moreover,the levels of fibrinogen(Fg),6-ketoprostaglandin f1alpha(6-Keto-PGF1?),thromboxane B2(TXB2) and P-selection(GMP 140) were determined in the four groups. Results The prevalence of Cpn IgG antibody was associated with AMI(OR 3.65).Adjustment for the major cardiovascular risk factors did not attenuate this correlation(OR 3.17). Those who were Cpn positive in the AMI group had significantly higher levels of Fg,TXB2 and GMP 140, and a significantly lower level of 6-Keto-PGF1? than those who were Cpn negative in the same group.Correlation analysis revealed that the average concentration of IgG antibody to Cpn was positively associated with the levels of Fg,TXB2 and GMP140 but negatively associated with the level of 6-Keto-PGF1? in the Cpn positive patients of the AMI group. Conclusions There is a significant correlation between Cpn infection and AMI. Cpn infection is associated with the levels of Fg,TXB2, 6-Keto-PGF1? and GMP140 in plasma.

From 1988 — 1990, the plasma 6 — keto —PGF_1. and TXB_2 were detected with radioimmunoassay in 100 cases with various types of viral hepatitis. The results showed thatTXB_2 and 6—keto— PGF_1. increase and PGF~./TXB_2 ratio decreased. There was clear difference as compaied with the normal. The value and PGF_1./TXB_2 ratio were correlated with jaundice, ascits, spontaneous peritonitis,and hepatorenal syndrome.

To investigate the effects of TGF?antisense oligodexynucleotides(ASODN) and EGFR ASODN on human rectal carcinoma cell line HR8348. Cationic liposome, lipofectamine, were selected as delivery vector HR8348 cells were treated with ASODN in vitro. HR8348 cells with or without pretreatment were inoculated into nude mice. The proliferation and DNA synthesis of HR8348 cells treated with TGF-? ASODN and EGFR ASODN were greatly inhibited, and tumorigenic rate was also greatly reduced. TGF-? ASODN and EGFR ASODN could inhibit the proliferation of human colon cancer cell line in vitro as well as in vivo, the results implicate. The potential value in colorectal cancer gene therapy.

patients with chronic alcoholic polyneuropathy (CAPN ) were described. They were all male with age rainging from 33 to 70 years (mean age 53. 7). The cardinal symptoms are. pain and numbness in the extremities, stocking and glove decreased of pain and temperature sense in the legs and arms, the loss of vibration sense and muscule atrophy. The motion nerve conducting velocity (MCV) and sensory nerve conduction velocity (SCV) all are slowed in varying degrees. Superficial peroneal nerve biopsy identified myelin degeneration of myelinated nerve fibers, axonal degeneration or disappearance, degeneration of Schwann cell and also involved perineurium. The change was due to avitaminosis for long-term drinking.

This paper introduces the technical parameters,basic principle and investigation method of the multiparameter supervisor.The functions and implementation methods of its components are also presented.The supervisor can provide much physiological information of the patients to medical staffs.Thus,the level of medical supervision and analysis can be enhanced.The multiparameter supervision can be realized when the microcomputer system with PC-104construction is adopted to analyze the received signals and filter the interfering signals resulting from the random variations of frequency and amplitude.

Objective To evaluate the therapeutic effect of combined splenorenal shunt and portaazygous devascularization on bleeding esophageal varices.Methods We summarized 120 cases suffering from esophageal variceal bleeding treated by splenorenal shunt plus portaazygods devascularization,from April,1978 to April,1997.Results The operative mortality was 4.2%,no short-term postoperative bleeding,and long-term postoperative bleeding rate was 9.5%,postoperative hepatic encephalopathy rate was 4.8%.Five,ten and fifteen years survival rate were 83.6%,64.5% and 54.5% respectively. The portal hemodynamics was observed by means of Doppler flowmeter,DSA,and intraoperative portal pressure measurement in some of the patients.Conclusion The result showed that combined shunt and portaazygous devacularization not only preserve the advantage of devacularization but also decrease the portal pressure and blood volume,alleviating portal hyperhemodynamics while preserve hepatopetal blood flow to some extent.

ObjectiveTo investigate the correlations between Herpes simplex virus (HSV) infection and acute myocardial infarction(AMI).MethodsSpecific antibodies and DNA to HSV-1 were measured in 51 patients with AMI(AMI group),42 patients with old myocardial infarction (OMI group) and 31 normal controls (NC group) who had no angiographic evidence of coronary artery disease.Moreover,the changes of and relationships between fibrinogen(Fg),6-Keto-prostaglandin-F 1? (6-Keto-PGF 1? ),Thromboxane B 2 (TXB 2 ),P-selection(GMP 140 ) and HSV-1 infection were determined in the three groups.ResultsThe prevalence of HSV-1IgG antibody was associated with AMI(OR4.266).Adjustment for the major cardiovascular risk factors didn't attenuate this correlation(OR3.321).Those who were HSV-1 positive in the AMI group had significantly higher levels of Fg,TXB 2 and GMP 140 and a significantly lower level of 6-Keto-PGF 1? than those who were HSV-1 negative in the same group.Correlation analysis revealed that the average concentration of IgG antibody to HSV-1 was positively associated with the levels of Fg,TXB 2 and GMP 140 but negatively associated with the level of 6-Keto-PGF 1? in the HSV-1 positive patients of the AMI group.ConclusionThere is a significant correlation between HSV-1 infection and AMI.HSV-1 infection is associated with Fg,TXB 2 ,6-Keto-PGF 1? and GMP 140 .

Objective To explore the efficacy of craniotomy and aneurysm clipping in elderly patients with ruptured intracranial aneurysms.Methods Totally 75 elderly patients with ruptured intracranial aneurysms in our hospital who underwent craniotomy and intracranial aneurysm clipping:approaching into head by wing point based on CT image,selecting the appropriate position and angle for clipping the aneurysm.Surgical efficacy and postoperative complications were observed.Prognosis was assessed by Glasgow Outcome Scale (GOS).Results 77 aneurysms presented in 75 patients and 76 aneurysms were clipped.The main postoperative complications were intracranial hematoma (5 cases),pulmonary infection (4 cases),electrolyte imbalance (10 cases),intracranial infection (2 cases),arrhythmia (3 cases),gastrointestinal bleeding (5 cases),cerebral infarction (3 cases).GOS assessment demonstrated that prognosis in 48 cases was good (64.0％),16 cases was moderate disability (21.3％),6 cases was severe disability (8.0％),2 cases was vegetative survival (2.7％),3cases was in death (4.0％).The cure rate was 64.0％ and the overall good-prognosis rate was 85.3％.Conclusions Craniotomy and aneurysm clipping has a better surgical efficacy and a high cure rate in elderly patients with ruptured intracranial aneurysm.

Abstract: In order to investigate immune protection against swine-origin influenza virus (S-OIV) A H1N1, the helper-dependent adenovirus vector (HDAd) system was exploited to construct recombinant HDAd encoding hemagglutinin (HA). The HA gene was synthesized and cloned to the HDAd backbone. Then, the HDAd/HA DNA molecules were transfected into 293Cre4 cells with calcium phosphate. The cells were infected by helper virus 16 hours after the transfection. The 293Cre4 cells were coinfected with HDAd/HA and the helper virus for large-scale preparation of HDAd/HA. The HDAd/HA was obtained and purified twice with CsCI density ultracentrifugation and observed morphologically under transmission electron microscope, and the expression of HA protein was analyzed with RTPCR. Recombinant HDAd/HA expressing HA protein was successfully constructed which could pave the way for in vivo investigation on immunogenicity and efficacy against S-OIV A H1N1 infection.
Adenoviridae ; Cell Line ; Cloning, Molecular ; Genetic Vectors ; Helper Viruses ; Hemagglutinin Glycoproteins, Influenza Virus ; biosynthesis ; Humans ; Influenza A Virus, H1N1 Subtype