By use of FITC-Con A made in China, the quantity of receptors on mouse thymocytes was determinated by saturation method and its affinity ocnstant was estimated. Afted the conditions of quantitative analysis with fluorescence method were defined, the quantity of receptors on thymocytes from 12 mice by two lots was carried out. The result demonstrated that the quantity of receptors per mouse thymocyte was 1.82?10~7 and its affinity constant K was 4.56?10~5M~(-1). This result was similar to radio-quantitative analysis of receptors, but without the disadvantage of radio-active contamination.

15 cases of malignant tumours of gastrointestinal tract and ovary were treated by chemotherapy through intraperitoneal puncture and perfusion under B-ultrasound guidance.The results were satisfactory.The success rate of intraperitoneal puncture under B-ultrasound guidance was 100%.The method is introduced in detail and the advantage of the application of B-ultrasound guid-ance was discussed.The authors considered that it is safe,reliable,less in complication,easy to oper-ate:less in agopy and easy to be used clinically.

Objective By analyzing the correlation between Doppler spectrum of extracranial cerebral vessels ECV and intracranial arterial stenosis IAS to identify the value of hemodynamic change of ECV in predicting IAS Methods One hundred and four patients with moderate to severe IAS or intracranial arterial occlusion were enrolled in the case group while 32 patients without obvious cerebrovascular disease in the same period were enrolled in the control group Ultrasound examinations of ECV were performed for all patients the hemodynamic parameters of bilateral common carotid artery CCA internal carotid artery ICA and vertebral artery VA were recorded and compared between case and control group Results Compared with control group the ECV of patients with IAS had lower velocity and higher resistance index RI Of all the parameters end diastolic velocity EDV of ICA and EDV of VA were most accurate in predicting IAS with best cut off value of 21 3 cm/s 9 6 cm/s and accuracy of 76 1 % 81 0% respectively Conclusions The ECV of IAS patients tend to have lower velocity and higher RI Analysis of hemodynamic parameters of ECV might be useful in predicting IAS.

Objective To compare the distance of left ventricular along long axis measuring under real-time movement and Doppler tissue imaging.Methods Titanium nips were implanted into anterior wall and anterior septum of left ventricule of 7 open-chest canine models.Quantitative study was made on time velocity integral(TVI) of the implanted myocardium and left ventricular long axis in 7 canine models,and 7 patients with replaced metal mitral valve.Digital subtraction angiography was adopted to record the real-time movement of the titanium nips in 7 canine models and the metal valve in 7 patients.And comparison was made between the results.Results The maximum values of displacement measuring with TVI were close to the distance under real-time movement. Conclusions The displacement measuring with TVI can reflect the character of the myocardial displacement truly.

OBJECTIVE To investigate the sterilizing effect of Avagard surgery wasting liquid.METHODS All the staff on operation were divided into two groups at random,each no less than 50 persons.Both groups were sterilized by 4% Avagard surgery washing liquid.In one group,hands and forearms were smeared by 0.5% Avagard fast surgery washing liquid(smearing group),the other group was not smeared by 0.5% Avagard fast surgery washing liquid(no-smearing group).In both groups the samples were derived from fingers and cultured right after sterilization and at intervals of 1h,2h,3h,4h,5h and 6h during operation,and to investigate the sterilization effect.RESULTS The bacteriological detections of both groups in all times showed that the number of colony was0.05).CONCLUSIONS The results of the experiment show that 4% Avagard surgery washing liquid is reliable for its sterilizing effect and easy to use.The duration of antibacterial effect is no less than 6 hours,this method could reduce the cost of the operation and improve the efficiency of work.

Objective To compare the differences of the four estimating equations about MDRD,CKD-EPI eGFRcr,CKD-EPI eGFRcys,and eGFRcr-cysin evaluating the renal function of kidney transplant recipients,and explore the optimal equations to estimate the renal allograft function.Method Between January 2000 to March 2015,a total of 213 renal transplant recipients with data integrity were enrolled.eGFR was calculated using the equations:Modification of Diet in Renal Dis-ease [eGFR (MDRD),the creatinine-based equation (CKD-EPI eGFRcr),the CKD EPI cystatin C equation (CKD-EPI eGFRcys),and CKD EPI creatinine + cystatin C equation (CKD-EPI eGFRcr-cys) drafted by Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) in 2009 and 2012.We assessed the performance of these four eGFR equations by Pearson correlation analysis,Bland-Altman plot,and Cox regression analysis.Result The incidence of reduced kidney function was 49.3％ using MDRD,43.7％ using eGFRcr,49.8％ using eGFRcys,and 52.1％ using eGFRcr-cys,respectively.In recipients with eGFR ≥60 ml·min· 1.73 m2 estimated by MDRD,19.5％ and 11％ had eGFR ＜60 ml·min·1.73 m2 re-estimated by eGFRcys and eGFRcr-cys respectively.Serum cystatin C was significantly correlated with serum creatinine,MDRD,eGFRcr,eGFRcr-cys with the correlation coefficient being 0.67,0.61,0.65,and 0.77 respectively.Only the CKD staging based on eGFRcr,and eGFRcr-cys was positively related to renal graft loss risk.eGFRcr,and eGFRcr-cys could not be replaced each other,and only eGFRcr-cys showed strong correlation with the other three equations.Conclusion As compared with others,eGFR (CKD-EPI-Creat + CysC) is more precise in estimating GFR and evaluating the renal graft loss risk.

Objective To elucidate the relationship between mandibular lateral displacement and mandible asymmetry by observing the morphological changes of condylar head and mandible during mandibular lateral displacement in growing rats.Methods Forty-eight male Wistar rats at the age of four weeks were divided at random into experimental and control groups,with 24 rats in each group.A super-hard resin plate was cemented to upper incisors to displace rat mandibles 2 mm to the left during closure(Ipsilateral side).A metal crown was fitted to lower incisors.The rats were killed 2,4,8 or 12 weeks after appliance attachment.The mandible was dissected out and halved.The length and width of condylar head were measured with a caliper.Radiographic films of the mandibles were exposed,and selected measurements were made.Results The length of condylar cartilage on the ipsilateral side was significantly larger than contralateral side in experimental group(P

Objective To probe the value of absent or reversed diastolic flow in hepatic artery shown by Doppler sonography of recently transplanted liver in predicting subsequent hepatic artery thrombosis.Methods The records of 54 liver transplantations performed were retrospectively reviewed.The first Doppler study of every case was done within 24 h after transplantation and subsequently reviewed.Findings were categoried into one of three patterns: forward flow,no flow and reversed flow throughout diastole.Meantime,the flow status of portal vein of every case was studied by Doppler sonography.Results Of 54 liver transplants, 12 had aberrant diastolic flow in the hepatic artery after liver transplantation.Four had reversed flow and 8 showed no flow in diastole.In this group of 12 transplants,hepatic artery thrombosis was not developed.Conclusions Reversed or absent diastolic flow in hepatic artery of a recently transplanted liver has no correlation with subsequent hepatic artery thrombosis.

Objective To evaluate the feasibility and clinical value of color Doppler flow imaging(CDFI) guided inferior vena caval filter insertion(IVCFI). Methods Thirty-one consecutive patients with unilateral lower extremity deep venous thrombosis were selected for IVCFI. Screening CDFI was performed in all the patients. Locations of renal veins (RV),maximum diameter of the inferior vena cava (IVC),and presence or absence of thrombus were documented. If visualization was adequate,IVCFI was performed under guidance of CDFI. CDFI and abdominal plain film of radiograph were used to document proper deployment,and circumferential engagement of the filter struts in the IVC wall. Also,CDFI was repeated each one month to assess IVC filter migration,thrombus adherent to the filter,and IVC patency. Results CDFI visualization was adequate in thirty-one patients(100%) and the average diameter of IVC was 19.7 mm. Thirty-one VenaTech IVC filters were placed without technical difficulty. No technical complication occurred in all the patients. Follow-up examination showed that no filter uncompleted opening and migration and no IVC thrombus were observed. Seven cases (29%) with embolus trapped by IVC filter were found. There had been no report of pulmonary emboli after IVCFI. Conclusions Placement IVC filter is feasible and safe with CDFI. CDFI-guided IVCFI substantially reduces the procedural cost and avoids the need for radiation exposure and intravenous contrast.