Object To study the anthraquinone constituents in rhizoma and radix of Rheum sublanceolatum C Y Cheng et T C Kao Methods The constituents were isolated through column chromatography, and their structures elucidated through physicochemical and spectral analysis Results 6 anthraquinones were isolated and identified as chrysophanol (Ⅰ); physcion (Ⅱ); emodin (Ⅲ); emodin 8 O ? D glucopyranoside (Ⅳ); aloe emodin 8 O ? D glucopyranoside (Ⅴ) and citreorosein (Ⅵ) Conclusion All these compounds were isolated from R sublanceolatum for the first time

Objective To understand the virus-carrying status and infection condition of Culicoides in Yunnan province.Methods Culicoides, cattle serum samples and pig serum samples were collected in Qujing City in Yunnan province from Sep.to Nov.in 2011; the supernatant of Culicoides was inoculated in C6/36 cells to isolate virus.Suspected isolates were identified by molecular biology techniques and titers of virus antibodies in pigs and cattles serum samples were tested by VN.Results 8 300 short tarsal Culicoides (8 300/18 160) and 7 100 Ryukyu Culicoides (7 100/18 160) were identified among 18 160 Culicoides specimens.Two suspected virus strains were isolated and designated as SC093 and SC233.The nucleotide sequence homology of VP2, VP4 and VP8 sequences with Banna virus Chinese strain ( Accession number:AF134526) is up to 99%.1 out of 200 (1/200) cattle serum samples was antibody positive against Banna virus with 0.5%positive rate.And the neutralizing antibody titers of SC093 and SC233 with above Banna virus antibody positive cattle serum were 160 and 40.10 out of 535 ( 10/535 ) pig serum samples were antibody positive against Banna virus with 1.7%positive rate.And the neutralizing antibody titers of SC093 and SC233 with above Banna virus antibody positive pig serum samples were 80-320 and 20-80 respectively.Conclusions The SC093 and SC233 virus strains isolated from Shizong were identified to be Banna virus and it could cause infection in pigs and cattles.This is the first report that Banna virus was isolated from Culicoides.

Objective:To compare the assay and related substance detection methods of erythromycin lactobionate in the Chinese Pharmacopoeia 2020(ChP),USP 2023(USP),JP18(JP),BP2023(BP)and EP 11.0(EP),investigate the differences between the test results obtained from 7 batches of erythromycin lactobionate for injection samples by using ChP and BP methods and thus provide a reference for the improvement of specification of erythromycin lactobionate.Methods:The differences of test methods and limits under the items of assay and related substances of erythromycin lactobionate in the above five pharmacopoeias were listed and compared.The related substances and contents of erythromycin lactobionate for injection samples from different manufactures were tested with methods stated in ChP and BP,and then compared and analyzed.Results:The items of related substances were determined by high performance liquid chromotographic methods in the ChP,EP and BP.The test methods and limits in the EP and BP were the same,which were different from that in ChP.The related substances were not determined in the USP and JP.There are obvious differences between the chromatographic methods and limits for the items of related substances in the ChP and BP,i.e.,BP contains 7 specific impuri-ties,while ChP contains only 2 specific impurities.The limits for any other impurity and the total amount of impurities were lower in the BP than those in the ChP.The antibiotic microbiological assay was used as the test method for the item of assay in the ChP,USP and JP based on different bacterial strains,which was different from the chromatographic method used in the BP.Based on the different methods of the ChP and BP,the related substances and content determination results of 7 batches of erythromycin lactobionate for injection samples met the acceptance criteria.The detection efficiency of BP related substances inspection method for specific impurities and total impurities were significantly higher than that of the ChP method.Conclusion:The BP method is superi-or to the ChP method in the detection of erythromycin lactobionate and erythromycin lactobionate for injection related substances.In terms of content determination,the external standard method adopted by BP is feasible to replace the antibiotic microbiological assay adopted by the ChP.Related substances and content determination methods adopted by the BP can provide an important reference for the revision and improvement of erythromycin lactobionate standard in the ChP.