Objective To observe the protective effect of magnesium gluconate on myocardial apoptosis by ischemia reperfusion injury in isolated rat hearts, and study the possible mechanism. Methods The hearts of 48 Sprague-Dawely rats were isolated, linked to Lange-ndorff perfusion apparatus, and randomly divided into 3 equal groups(n = 16 each) : Control group, ischemia/reperfusion (I/R) group and magnesium gheonate group. 8 rats in each group were perfused. Control group was pedused with modified KH buffer for 110min. I/B group was perfuesd with modified KH buffer for 20 min, then exposed to iscbemia for 30 min, and then reperfused with modified KH buffer for 60 min. Magnesium gheonate group was perfumed with modified KH buffer with magnesium gluconate for 20 min, then exposed to isohemia for 30 min and then reperfused with modified KH buffer with magnesium glueonate for 60 min. Lacate dehydrogenase (LDH) and ereatine kinase (CK) in the effluent liquid from the heart were measured after reperfusion. The concentration of Ca2+ and NO in the left ventricle were determined. The other 8 rats in each group were reperfused for 120 minutes as the method described before. After repeffusion, the myoeyte apoptosis was examined by Annexin-V-FITC/PI. After the two experiments the incidence of ventrieular arrhytlunias during reperfusion was assessed. Results Compared with I/R, magnesium glueonate decreased the incidence of ventricular an'hythmias(P <0. 01). The contents of CK and LDH in the effluent liquid from the heart in magnesium glueonate group was lower than that of I/R group (P <0. 01). The contents of Ca2+ and NO in the left ventricle in magnesium gluconate group was decreased than that of I/R group (P <0. 01). The index of myocyte apoptosis were significanfly lower in magnesium glueonate group than that of I/R group (apoptosis index :27.79±1.59 vs 33.61±2.10, P < 0. 01) . Conclusion Magnesium glueonate has protective effect on myocardial isohemia reperfusion injury in rats. The protective effect may be related to decreasing myocyte apoptosis by increasing the content of NO and relieving calcium overload.

Objective:This study investigates the expression of long non-coding RNA termed amine oxidase, copper containing 4, pseudo-gene (AOC4P) in hepatocellular carcinoma (HCC) and its relationship with clinicopathological factors and patient prognosis. Methods:The expression of AOC4P in 106 HCC tissues and adjacent non-tumor tissues were detected using qRT-PCR. The relationship between AOC4P expression and clinicopathological factors, as well as patient prognosis, in HCC was analyzed. Results:The relative expression of AOC4P in the cancer tissues was 0.66±0.34, which was significantly lower than the relative expression in adjacent non-tumor tissues, i. e., 1.45±0.63 (P<0.05). Univariate analysis showed that low AOC4P expression was correlated with tumor size, lymph node metastasis, and TNM stage (P<0.05). The group with high AOC4P expression levels has longer survival time compared with the group with low AOC4P expression levels. Cox multivariate regression analysis showed that AOC4P expression, lymph node metastasis, and vascular in-vasion were all independent factors that affect prognosis of patients. Conclusion:AOC4P expression was reduced in HCC tissues, and AOC4P may play crucial roles in the occurrence and development of HCC. For patients with poor prognosis, low AOC4P expression is an independent risk factor for patients with HCC. Thus, AOC4P expression may be used as a molecular marker for HCC.

Objective To discuss the value of multi-slice spiral CT angiography (MSCTA)and digital subtraction angiography (DSA)in lower extremity arteriosclerosis obliterans.Methods MSCTA and DSA of lower extremity arteries were performed in 41 patients with lower extremity arteriosclerosis obliterans sequentially.The interval of these two examinations was no more than 2 weeks.DSA served as the reference standard and compared with MSCTA in terms of grading of stenoocclusive lesions,visualization of collaterals in lower extremity arteries.Results No significant differences of grading of stenoocclusive lesions,visualization of col-laterals were observed between MCTA and DSA in lower extremity arteries.Compared with the results of DSA,the sensitivity,spe-cificity,accuracy,positive and negative predictive value of MSCTA in the detection of arterial stenosis (≥ 50%)were 97.2%, 98.2%,93.8%,94.6%,98.2%,respectively.Conclusion MSCTA shows relatively high sensitivity and specificity in the diagnosis of lower extremity arteriosclerosis obliterans,can provide effective guidance of planning treatment in lower extremity arteriosclerosis obliterans.

Objective To explore the reproductive outcomes of different management strategies of ectopic pregnancy .Methods A retrospective cohort study was performed for 648 consecutive patients with a diagnosis of ectopic pregnaney between June 2011 and september 2013.The follow-ups were conducted telephone interviews of pregnancy outcomes after various treatments .Results The subsequent intrauterine pregnancy rates after surgical , medical and expectant managements were 89(65.0%), 35(79.6%) and 6 (54.5%) the intrauterine pregnancy was higher for medical treatment than that for surgical treatment (P<0.05).The subsequent infertility rate after surgical , medical and expectant management were 4.5%, 36.4%, 19.0%.The subsequent infertility rate after medical treatment was lower than that for surgical and expectant managament ( P<0.05) .The re-ectopic pregnancy rates of laproscopic versus abdominal operation were [11 (11.7%), 13(30.2%)] with significant difference (P<0.05).And abdominal operation was higher than laproscopic operation .Conclusion For younger ectopic pregnancy patients , medical treatment shold be as possible as we can .If operation is necessary , laproscopic operation is preferred .

Objective To explore the role of cell proliferation and apoptosis, and the expression significance of involved protein as Bcl-2(B-cell lymphoma/leukemia-2) and Bax (Bcl-associate X protein) in the developing small intestine of human fetus. Methods The expression product of Bcl-2 and Bax was investigated with immunohistochemical methods in the 2nd, 3rd and 4th months of gestation respectively. Results In the second, the third and the fourth month of gestation, the Bcl-2 immunoreactive positive signals were found in the ganglion cells of intermuscular and submucous nerve plexus. Bax positive cells were observed in the cytoplasm of the simple columnar epithelium cells of the small intestinal mucous layer.Conclusion Bcl-2 and Bax proteins regulate the developing small intestine of human fetus.

Objective To study the ches t X-ray and CT appearance of pulmonary nontuberculosis mycobacteria (NTMB) Methods The chest X-ray findings of 22 cases with cultures positive for pulmonary nontuberculosis mycobacteria were reviewed, 10 cases had CT scans simultaneously All abnormalities and predominant lobar involvement were recorded The findings of chest X-ray and CT were compared Results The chest X-ray showed that air space consolidation ( n =19) and cavities ( n =18) were most frequently seen, nodules ( n =12) and linear disease ( n =14) were observed too The abnormalities involved bilateral multiple lobes, the right lung was more frequently involved than left, and upper lobe was involved more than lower ones Multiple manifestations were often co-existing ( n =19) On CT scans, nodule was the most frequent finding ( n =10), air space consolidation ( n =8) and cavity ( n =8) were other common findings In addition, bronchiectasis ( n =5), “tree in bud” sign ( n =3), and mediastinal lymphadenopathy ( n =2) were also seen on CT but not on chest X-ray Conclusion Air space consolidation, cavity, nodule, fibrosis, bronchiectasis, and “tree in bud” are major abnormalities of chest image The image abnormalities of pulmonary NTMB are indistinguishable from those of secondary pulmonary TB Multiple co-existing manifestations may be the characteristic findings of pulmonary NTMB infection

ObjectiveTo study the effect of exercise on expression of p38 after cerebral infarction in rats.MethodsThe right middle cerebral artery was occluded(MCAO) with electric coagulation in Wistar rats,and then were divided into three groups at random 48 hours later: training group which were given wheel-running exercise everyday(n=20);control group(n=20) and sham group(n=10).The expression of p38 in cortex of the three groups were detected on 7th,14th,21th and 28th day after the MCAO.ResultsIn the cortex around the infarcted focus,the expressions of p38 elevated much significantly after cerebral infarction(P<0.05).The expression of p38 in training group were higher than those in control group(P<0.05) on the 21th and 28th day.ConclusionThe cerebral infarction could induce the expression of p38.The exercise could enhance its expression.

Objective To evaluate clinical application of thyroid fine-needle aspiration biopsy (FNAB) and its diagnostic value.Methods FNAB was performed for 782 cases during 2005-2009 at China-Japan Friendship Hospital,Beijing and their clinical pathological data were retrospectively analyzed.Cytopathological diagnoses by FNAB were classified as unsatisfactory,benign,atypical cellular lesions,follicular neoplasm,suspicious malignancy and definite malignancy.Results of thyroid cytological and histological examinations of 76 patients with subsequent thyroidectomies were compared to those with FNAB to evaluate its diagnostic accuracy.Results Of 782 FNAB specimens,74.4 % (582/782) were classified as benign,2.6 % (20/782) as malignant,5.6 % (44/782) as follicular neoplasm,3.2 % (25/782) as atypical cellular lesion,2.9% (23/782) as suspicious malignancy and 11.3 % (88/782) as unsatisfactory.Among 76 patients with thyroidectomies,65.8 % of them (50/76) were histologically diagnosed as benign lesion,28.9 % (22/76) as definite malignancy and only 5.3 % (4/76) as benign follicular adenoma.Agreement between FNAB cytological diagnoses and histologically confirmed diagnoses for benign lesion and malignancy was 16/16 and 16/17,respectively.Conclusion FNAB is a reliable diagnostic method for evaluation of thyroid nodules.

0. 05) ; whereas Herrmann Ⅲ ,Ⅳand T3 ,T4(depth of invasion) were more often seen in advanced signet cell carcinoma (P 0. 05 ). Conclusion Compared with other pathological types, patients with advanced signet ring cell carcinoma of the stomach are with comparable long term survival.

Objective To explore the correlation between COX-2 and Ki-67 expression and microvessel density in primary gastric cancer tissue and clinicopathological parameters and to investigate their effect on postoperative gastric cancer recurrence. Methods Forty-eight postoperative recurrent gastric cancer patients were enrolled in this study, their clinicopathologic characters of primary tumors were analyzed retrospectively and compared with 48 cases without recurrence. Expression of COX-2 and Ki-67 and microvessel density ( MVD) in gastric cancer tissue were detected by immunohistochemical method. Risk factors correlated with tumor recurrence were studied by univariate and multivariate analysis. Results Expression of COX-2, Ki-67 label index (LI) and MVD were correlated with each other, and they were all correlated with depth of invasion and TNM stages in gastric cancer tissue. Patients with pelvic metastatic lymph node, cancer invasion to serosa, of Borrmann III or IV, high COX-2 and MVD expression are on high risk of recurrence (P