Objective To explore the feasibility and clinical significance of combined detection of human plasma and feces expression level of microRNA-92a-1 (miRNA-92a-1) as colorectal tumour screening marker.Methods From August to October in 2011,the feces and plasma samples of 60 colorectal cancer (CRC) patients,23 colorectal adenoma (CRA) patients and 30 healthy controls were collected.The expression level of miRNA-92a-1 was determined by quantitative reverse transcription-polymerase chain reaction.Mann-Whitney's U test was applied for the difference test between groups.Then,according to the receiver operating characteristic (ROC) curve analysis the cut-off point was determined and the sensitivity and specificity were analyzed.Results Compared with healthy controls,the expressions of miRNA-92a-1 in plasma of CRC and CRA patients increased (U=288.5 and 151.0,both P＜0.01).Compared with healthy controls,the expression of miRNA-92a-1 in feces of CRC patients increased (U=627.5,P=0.0199).According to ROC curve analysis,when the cut-off point was ＞1.22,the sensitivities of plasma miRNA-92a-1 in CRC patients and CRA patients were 85.0％ (51/60) and 73.9％ (17/23) respectively.The specificity of healthy controls was 76.7％ (23/30).When the cut-off point was ＞1.14,the sensitivities of feces miRNA-92a-1 in CRC patients and CRA patients were 31.7％(19/60) and 26.1％(6/23) respectively.The specificity of healthy controls was 90.0 ％ (27/30).Combined the detection results of plasma and feces,the sensitivities of miRNA-92a-1 in CRC patients and CRA patients was 88.3％ (53/60) and 82.6％ (19/23) respectively.The specificity in healthy controls was 73.3％ (22/30).The sensitivity was higher than that of single sample testing.Conclusions The sensitivity and specificity are high in the combined detection of plasma and feces miRNA-92a-1 expression level in CRC patients and CRA patients,miRNA-92a-1 may be a potential CRC screening marker.

Objective To analyze the clinical features and to explore the potential mode of genetic transmission of vitiligo vulgaris in Chinese Han population. Methods Information on the clinical features and the familial history of patients with vitiligo vulgaris was collected by a uniform questionnaire. The data were inputted into a data base, then analyzed by Epi Info 5.0 and SPSS 10.0 software packages. A complex segregation analysis was conducted using the REGTL program in SAGE 3.1 package in order to propose the putative mode of genetic transmission for vitiligo vulgaris. Results The mean age of onset of vitiligo vulgaris of the males and females was about 19 years old. In the severity of the disease and the season of onset, there was no significant difference between males and females. For the patients with vitiligo vulgaris, there was a female preponderance of complicating with autoimmune disorders, especially hyperthyroidism. The prevalence rates of vitiligo in patients′ relatives were significantly higher than that in general population. The mode of transmission of vitiligo vulgaris was one of the multifactorial inheritance according to the results obtained by the complex segregation analysis. Conclusions There are no differences in the prevalence and the age of onset between males and females patients. There is a marked familial aggregation in the patients with vitiligo vulgaris. The familial clustering might result from the polygenic additive genetic background and common environmental factors. It is suggested that vitiligo vulgaris be a multifactorial complex disease.

Children and adults differ significantly in physiology,biochemistry and immune function,which leads to sig-nificant differences in blood transfusion strategies between children and adults.To guide the clinical transfusion practice of pediatric patients and improve the prognosis of children,the National Health Commission organized the formulation and re-lease of the health industry standard Guideline for Pediatric Transfusion(WS/T 795-2022).This paper will briefly introduce some concepts that help understand of the Standard and the preparation process of the Standard,and explain and interpret the preparation of the"scope","general provisions"and"factors to consider"of the Standard,hoping to contribute to the understanding and implementation of the Standard.