1.Target volume delineation for glioblastoma multiforme: current practice and advice
Minghuan LI ; Li KONG ; Jinming YU
Journal of International Oncology 2013;(3):193-196
The use of adjuvant extemal-beam RT is well established in the postoperative treatment of glioblastoma multiforme (CBM).It is consensus that target volume should be determined based on the fusion images of MRI and CT,but the inclusion of peritumoural edematous is controversial.The vast majority of recurrences occur within 2 cm of the original tumor site orin radiation field.There is no inevitable relation between the degree of peritumoral edema and recurrence model.The clinical and pathological characteristics may be as predictive and prognostic factors for the treatment of GBM.Target volume delineation for CBM tend to individual,which can maintain known outcomes and reduce treatment toxicity.
2.A Novel Biomimetic Porphyrin Biosensor Based on Poly (3,4-ethylenedioxythiophene) and 1-Pyrenebutanoic Acid Decorated with Hematin and Its Application in Bioelectrical Catalysis of Catechol
Xuehua YU ; Jinming KONG ; Lianzhi LI ; Xueji ZHANG
Chinese Journal of Analytical Chemistry 2014;(10):1400-1407
A new biosensor was prepared based on conductive polymer poly ( 3 , 4-ethylenedioxythiophene ) ( PEDOT) and 1-pyrenebutanoic acid ( PBA) through π-π stacking, as well as hematin associated with PBA through coordinate bonds of Zr4+ and carboxyl group. Its stability and sensitivity were examined by cyclic voltammetry ( CV) , electrochemical impedance spectroscopy ( EIS) and current-time ( i-t) method. A pair of well defined and quasi-reversible redox peaks was observed when GCE/PEDOT/PBA/hematin was tested by CV in PBS without oxygen. The electron transfer rate constant was estimated to be 4. 8 s-1 . Results indicated that the PEDOT film enhanced the electron transfer process of hematin. When the i-t method was used to detect the response of biosensor to catechol with different concentrations, it displayed a linear response for the reduction of catechol in the range of 0. 5-200 μmol/L. The linear equation was i=0. 018C+0. 006 ( R=0. 9998), and the detection sensitivity was 0. 258 μA (μmol/L· cm2) with a detection limit of 0. 33 nmol/L (S/N=3). The results illustrate that the GCE/PEDOT/PBA/hematin biosensor is very sensitive and stable.
3.Insulin enhances glucose transporter 1 gene expression of low-flow ischemic myocardium
Renfu YIN ; Jinming CHEN ; Zonggui WU ; Shaohua QIU ; Yongmei WANG ; Ruimei WU ; Xiantao KONG
Chinese Journal of Pathophysiology 2001;17(6):531-533
AIM: To investigate the mechanism underlying insulin-stimulated increase in glucose uptake during low-flow myocardial ischemia. METHODS: The expression of myocardial GLUT1 polypeptide was determined by semiquantitative immunoblotting. The expression of GLUT1 mRNA was determined by semiquantitative Northern blotting. RESULTS: After infusing insulin during low- flow myocardial ischemia for 8 h,the expression of both GLUT1 mRNA and GLUT1 polypeptide was significantly higher in experimental myocardium than that in normal myocardium. The glucose uptake was upregulated at the same time in the exprimental myocardium. CONCLUSION: Insulin enhances the expression of GLUT1 mRNA and GLUT1 polypeptide in ischemic myocardial regions. GLUT1 expression may be an important mechanism by which myocardial cells enhance glucose uptake and metabolism during low-flow myocardial ischemia.
4.Value of regular and dual-time-point FDG PET/CT imaging in detecting recurrence and metastasis after a curative esophagectomy in esophageal carcinoma
Wenjian LIU ; Li KONG ; Man HU ; Jinming YU ; Anqin HAN ; Li MA
Chinese Journal of Radiation Oncology 2011;20(1):36-39
Objective To evaluate the clinical value of regular and dual-time-point 18-fluorodeoxyglucose positron emission tomography-CT(FDG PET/CT)imaging for recurrence and metastasis in esophageal carcinoma(EC)after curative esophagectomy. Methods A retrospective study was done on 48 patients received curative esophagectomy, who underwent FDG PET/CT scans to detect doubtful recurrent or metastatic lesions. The diagnostic accuracy of FDG PET-CT was assessed with the help of pathological findings as well as clinical or follow-up data. Using Fisher's Exact Test from SPSS 11.5 to analyze the data.Results Of the 48 patients, after a median follow-up of 21.5 months, 61 sites of local and regional recurrence or metastasis were finally confirmed in 34 patients. The sensitivity, specificity and accuracy of regular FDG PET/CT imaging in detecting recurrence of all sites were 93.44%, 74.29% and 86.46%respectively. The specificity and accuracy of local recurrence and regional metastasis were 57.14% ,78.95% and 77.78% ,84.62%, respectively. The sensitivity, specificity and accuracy of dual-time-point FDG PET/ CT imaging in detecting local and regional recurrence(96.97% ,96.00% and 96.55%)were higher than those of regular FDG PET/CT(90.90%, 72.00% and 82.76%)and there were significant differences of specificity and accuracy(P = 0.049, P = 0.029). Conclusions Regular FDG PET/CT imaging is highly effective in detecting recurrence and metastasis in EC patients after curative esophagectomy despite the low specificity and accuracy. Dual-time-point FDG PET/CT imaging can elevate the specificity and accuracy.
5.Insulin facilitates glucose transporter 4 gene expression in canine heart in vivo
Renfu YIN ; Jinming CHEN ; Zonggui WU ; Shaohua QIU ; Yongmei WANG ; Ruimei WU ; Xiantao KONG
Immunological Journal 2001;(2):132-134
Objective To investigate the mechanism for that insulin facilitates increase of glucose uptake. Methods The expression of myocardial GLUT4 polypeptide was determined by semiquantitative immunoblotting. The expression of GLUT4 mRNA was determined by semiquantitative Northern blotting. Results After infusing insulin for 8 hours,the expression of GLUT4 mRNA and GLUT4 polypeptide was significantly higher in canine myocardium than in those found normal ones. The glucose uptake was upregulated at the same time.Conclusions Our findings suggest that insulin facilitates the expression of GLUT4 mRNA and GLUT4 polypeptide in canine hearts. Enhanced GLUT4 expression is one of the important molecular mechanism by which myocardial cells enhance glucose uptake by insulin stimulation.
6.Insulin enhances glucose transporter 1 gene expression of low-flow ischemic myocardium
Renfu YIN ; Jinming CHEN ; Zonggui WU ; Shaohua QIU ; Yongmei WANG ; Ruimei WU ; Xianta KONG
Chinese Journal of Pathophysiology 1989;0(06):-
AIM: To investigate the mechanism underlying insulin-stimulated increase in glucose uptake during low-flow myocardial ischemia. METHODS: The expression of myocardial GLUT1 polypeptide was determined by semiquantitative immunoblotting. The expression of GLUT1 mRNA was determined by semiquantitative Northern blotting. RESULTS: After infusing insulin during low- flow myocardial ischemia for 8 h,the expression of both GLUT1 mRNA and GLUT1 polypeptide was significantly higher in experimental myocardium than that in normal myocardium. The glucose uptake was upregulated at the same time in the exprimental myocardium. CONCLUSION: Insulin enhances the expression of GLUT1 mRNA and GLUT1 polypeptide in ischemic myocardial regions. GLUT1 expression may be an important mechanism by which myocardial cells enhance glucose uptake and metabolism during low-flow myocardial ischemia. [
7.Prognostic value of fluorodeoxyglucose positron emission tomography-computed tomography before and after radiotherapy in locally advanced nasopharyngeal carcinoma
Peng XIE ; Jinbo YUE ; Xindong SUN ; Rui FENG ; Li KONG ; Zheng FU ; Jinsong ZHENG ; Xianguang ZHAO ; Jinming YU
Chinese Journal of Radiation Oncology 2009;18(5):347-350
etabolic response to radiotherapy may predict the prognosis of paitents with locally advanced NPC. The prognosis is poor for patients with high FDG uptake before and after radiotherapy or SUV max-NSUV max-P .
8.Experimental study on preventive and therapeutic effec ts of 5 radioprotectants on combined radiation-burn injured mice
Chaohua GUO ; Peiyan KONG ; Zhongmin ZOU ; Yanhong ZHOU ; Jinming ZHOU ; Yong ZHANG ; Cunmeng SHI ; Xinze RAN ; Chengji LUO
Journal of Third Military Medical University 2001;23(5):544-546
Objective To compare the irradiation-protective and inter-synergestic effects of E838,WR-2721, Rubia cordifolia, cystamin e hydrochloride and ethinyl estradiol on radiation and combined radiation-burn injury. Methods Above-mentioned drugs were given to the mice i ntraperitoneally, or intragastrcally, then, the mortality and the average surviv al d for 30 d were observed before and after the administration of the drug s. Results ①When drugs were before injury , the survival rate and the average survival d of the radiation and combined radiation-burn injured mice were increased obviously with the best effect in E838 and WR-2721. ②When drugs were given after injury, E838 and R. cordifolia also kept the effect. ③Combined appling WR-2721(pre) and E838(post)displayed a significant syner gistic reaction. Conclusion E838 and WR-2721 are more e ffective than the others in the prevention of radiation.
9.The effect of pitavastatin on blood glucose and its efficacy in diabetic patients with hypercholesterolemia
Yong MAO ; Jinming YU ; Fen ZHANG ; Dayi HU ; Rongjing DING ; Yiqiang ZHAN ; Shechang LI ; Qunyu KONG ; Fanli LIN ; Gongxian JIA
Chinese Journal of Internal Medicine 2012;51(7):508-512
Objective To evaluate the effect of pitavastatin on blood glucose in patients with hypercholesterolemia,and to investigate the efficacy of pitavastatin in diabetic patients combined with hypercholesterolemia.Method This study was a 12-week,multi-center,open-label,without parallel-group comparison,phase Ⅳ clinical trail.Results Contrasting to baseline,the prevalences at week 4 and 12 post-treatment of abnormal fasting plasma glucose (FPG) and glycosylated hemoglobin Alc (HbA1c)( FPG:14.2% vs 14.1% and 11.0% ; HbA1c:14.3% vs 15.1% and 16.1% ) in the safety set subjects without diabetes mellitus (DM),as well as in those with DM but not taking glucose-lowering drugs (FPG:7/7 vs 4/7 and 5/7; HbAlc:5/5 vs 4/4 and 5/5) had no significant changes (all P vaules >0.05).Contrasting to baseline,the levels of TC [ (6.51±0.94) mmol/L vs (5.12 ±0.93) mmol/L and (4.54 ±1.00) mmol/L],LDL-C [(4.11 ±0.79)mmol/L vs (3.02 ±0.81) mmol/L and (2.51 ±0.70)mmol/L] and TG [2.10(1.53,2.54) mmol/L vs 1.62(1.26,2.00) mmol/L and 1.35(1.10,1.86)mmol/L]at week 4 and 12 post-treatment in the per protocol set 55 subjects with DM were significantly reduced (all P values < 0.05 ) ; 33.3% of subjects at high risk and 10.0% of subjects at very high risk had achieved a TC target value; 55.6% of subjects at high risk and 40.0% of subjects at very high risk had achieved a LDL-C target value.Conclusion Pitavastatin has a safe effect on blood glucose and it could be used to treat diabetic patients combined with hypercholesterolemia in China.
10.EMP3 Overexpression in Primary Breast Carcinomas is not Associated with Epigenetic Aberrations.
Wei ZHOU ; Zheng JIANG ; Xingang LI ; Fenghua XU ; Yanbing LIU ; Peie WEN ; Li KONG ; Ming HOU ; Jinming YU
Journal of Korean Medical Science 2009;24(1):97-103
Epithelial membrane protein 3 (EMP3) is a trans-membrane signaling molecule with important roles in the regulation of apoptosis, differentiation and invasion of cancer cells, but the detailed is largely still unknown. We analyzed the mRNA levels and methylation statuses of EMP3 in 63 primary breast carcinomas and assessed their correlations with clinicopathologic variables. The expression of EMP3 mRNA in primary breast carcinomas was significantly higher than the expression of 20 normal breast tissues (p<10(-7)). EMP3 overexpression in breast carcinomas was significantly related to histological grade III (p=3.9X10(-7)), lymph node metastasis (p= 0.003), and strong Her-2 expression (p=3.3X10(-6)). Hypermethylation frequencies of EMP3 were detected in 36.5% of breast carcinomas by methylation-specific polymerase chain reaction. However, no significant correlations were found between methylation status of EMP3 and mRNA expression levels as well as other clinical parameters. In conclusion, EMP3 may be a novel marker of tumor aggressiveness. Overexpression of EMP3 in primary breast carcinoma is not associated with DNA methylation.
Adult
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Breast Neoplasms/*genetics/pathology
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Carcinoma/*genetics/pathology
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DNA Methylation
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Epigenesis, Genetic
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Lymphatic Metastasis
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Membrane Glycoproteins/*genetics/metabolism
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Middle Aged
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Neoplasm Staging
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RNA, Messenger/metabolism
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Receptor, erbB-2/genetics/metabolism
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Severity of Illness Index