AIM:To investigate the changes of apoptosis and activation of I??-? induced by vinblastine via the blockage of caspase-3 signal transduction pathway, and to explore the possible mechanism of signal transduction pathway involving in the vinblastine-induced apoptosis. METHODS: The breast cancer cell lines Bcap37 were treated with different concentrations of vinblatine dissolved in dimethyl sulphoxide (DMSO) or caspase-3 inhibitor (DEVD-CHO, 100 ?mol/L) for 3 h. The changes of the proliferation were detected by MTT methods. The apoptosis was determined by observing the internucleosomal DNA cleavage and PI staining, and the proteins of pro-caspase-3 and I??-? were detected by Western blotting methods. RESULTS: The results showed that vinblastine induced the pro-caspase-3 degradation. The significantly attenuation of vinblastine-induced apoptosis in breast cancer cell line by caspase-3 inhibitor DEVD-CHO was verified by MTT assay, internucleosomal DNA cleavage and flow cytometry PI staining analysis. The IC50 was 56.8 ?mol/L and 87.4 ?mol/L respectively for two groups. The inhibition of vinblastine-induced phosphorylated degradation of I??-? was also observed by DEVD-CHO. CONCLUSION: Based on these finding, vinblastine induces apoptosis in breast cancer cells via NF-??/I?? signal transduction pathway, which is co-operated by caspase signal pathway. Through the blockage of caspase pathway with caspase-3 inhibitor, vinblastine-induced apoptosis and the phosphorylated degradation of I??-? in breast cancer cells are suppressed greatly.

AIM: To investigate the expression of drug resistance genes, MDR1 and MRP, in patients with primary breast cancer after neoadjuvant chemotherapy. METHODS: MDR1 and MRP gene expression were detected by semi-quantitative RT-PCR in 20 patients with primary breast cancer before and after chemotherapy. RESULTS: Before chemotherapy, MDR1 and MRP expression could be detected in 15 cases (75%) and 18 cases (90%), respectively. After chemotherapy, expression of MDR1 was not significantly different from that before chemotherapy, but expression of MRP was significantly different from that before chemotherapy. CONCLUSION: Drug resistance gene MRP, but not MDR1 expression is enhanced in patients with primary breast cancer subjected to neoadjuvant chemotherapy.

AIM: To investigate the expression of angiopoietin-Ⅱ(Ang-Ⅱ)in primary gastric cancer and the pathological factors that influences it. METHODS: Expression of Ang-Ⅱ and VEGF were studied in 72 primary gastric cancer and adjacent normal tissue by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry. RESULTS: The significant difference of Ang-Ⅱ expression between primary tumor and adjacent normal tissue samples was observed. The correlationship between Ang-Ⅱ and VEGF expression in tumors was statistically significant. The expression of Ang-Ⅱ was related to tumor stage and vascular involvement. CONCLUSION: The results manifested that Ang-Ⅱ may play a role in regulating tumor angiogenesis.

AIM: To construct pcDNA3.1+/MAGE-3 DNA vaccine and investigate the antigen-specific antitumor immune responses induced by pcDNA3.1+/MAGE-3 DNA vaccine in vivo. METHODS: C57BL/6 mice challenged with B16/MAGE-3 cells were immunized by intramuscular injection of pcDNA3.1+/MAGE-3 DNA vaccine every 10 days. pcDNA3.1+ plasmid and PBS were used as controls. After three cycles of immunization, murine splenic lymphocytes, serum, and tumor were obtained for cytotoxity assay, detections of cytokines (IL-2 and IFN-?), measurement of MAGE-3 antibody, and tumor inhibitory rates, respectively. RESULTS: The pcDNA3.1+/MAGE-3 DNA vaccine immunized murine lymphocytes induced specific cytotoxicity against B16/MAGE-3 cells. Significantly increased secretions of IL-2 and IFN-? were detected. The titres of antibody against MAGE-3 were 1∶1 and 1∶20, while controls were negative. The tumor inhibitory rate in pcDNA3.1+/MAGE-3 group was significantly different from that in controls. CONCLUSION: The pcDNA3.1+/MAGE-3 DNA vaccine was constructed successfully. pcDNA3.1+/MAGE-3 DNA vaccine activates both cellular and humoral immune responses, and induces antigen-specific antitumor immune responses in vivo. [

BACKGROUND:Fluoride treatment of osteoporosis has been controversial.Literatures addressing the effect of fluoride on bone bio-mechanical parameters of femur in young rats are few.OBJECTIVE:To study the effects of fluoride on bone biomechanical parameters of femur in young rats.METHODS:Ninety 2-month-old SPF Sprague Dawley rats,half male and female,were randomly divided into 9 groups:control group(young,adult and long-time)and drug-administered group(young high-fluoride,young low-fluoride,adult high-fluoride,adut low-fluoride,long-term high-fluoride and long-term low-fluoride).Rats in the control group were orally administered with physiological saline,while in the drug-administered group were given orally with different dose fluoride at the corresponding times.After experiment,rats were sacrificed under anaesthesia.Three-point bending test was performed at the left femur.The effects of fluoride on maximum load and rigidity of femur were measured.RESULTS AND CONCLUSION:Compared with young control group,the maximum load and the rigidity of femur in the young high-fluoride group were decreased by 13.18%and 13.61%,respectively(P＜0.05),which had no dramatically difference in the young low-fluoride group.Compared with long-term high-fluoride group,the maximum load and the rigidity offemur in the young high-fluoride were decreased by 17.22%and 17.17%(P＜0.05),which were obvious increased in the long.term low-fluoride grou by 18.33%and 19.15%,respectively(P＜0.05).The maximum load and the rigidity of femur were strengthened in the adult high-fluoride and adult low-fluoride groups(P＜0.05).The results suggested that young rats are more sensitive to high-dose fluoride,which can reduce bone quality in rats.The negative effects on bone quailty of rats were gradually displayed as the prolongation of the period of fluoride.

AIM: To evaluate the influence of radiofrequency (RF) hyperthermia on immunity function in mice. METHODS: The expression pattern of T helper type 1 (Th1) and T helper type 2 (Th2) cytokines in splenocyte culture supernatants, mainly the expression levels of IFN-?, IL-2, IL-4 and IL-10 in splenocyte culture supernatants of mice in tumor-bearing group, surgical resection group, RF therapy group and normal control group were detected by enzyme-linked immunoadsordent assay (ELISA). RESULTS: IL-2 concentration in two weeks after RF therapy group was higher than that in two weeks after surgical resection and normal control groups (P0.05). CONCLUSION: RF hyperthermia may activate the transformation from Th2 to Th1 and facilitate the excretion of Th1 type cytokines that play an important role in the anti-tumor immunity.

[Summary] A 32 year-old woman in the third trimester of pregnancy was admitted for severe acute pancreatitis due to hypertriglyceridemia . During hospitalization she developed multiorgan dysfunction , infected pancreatic necrosis , abdominal compartment syndrome and intrauterine fetal death . She was successfully treated by multidisciplinary team including department of emergency medicine , ICU, gastroenterology, obstetrics, endocrinology, ultrasonography, radiology, infectious disease, nutrition and surgery.

AIM:To observe the effect on NF-?B pathway and cell motility in breast cancer cell lines after transfection of dominant negative I?B? plasmid.METHODS:After stable transfection of mutant I?B? plasmid into highly metastatic breast cancer lines MDA-MB-231 and MDA-MB-435,we detected NF-?B binding activity by EMSA,cell growth ability by cell growth curve,colony forming test,and cell motility by millicell-PCF chamber.RESULTS:Constitutive activities in MDA-MB-231 and MDA-MB-435 were observed.Stable transfection of a dominant negative Ⅰ?B? resulted in downregulation of NF-?B binding activity,thus inhibited cell mobility without significant effect on cell growth.CONCLUSION:Cell migration ability is inhibited in highly invasive breast cancer cells by inhibition of NF-?B pathway in vitro.

Objective To investigate the effects of crude extracted proteins from lesions of condyloma acuminata on immunophenotypes of dendritic cells.Methods Plastic-adherent mononuclear cells(MNCs)were isolated from umbilical cord blood or peripheral blood and cultured in media containing cytokines(GM-CSF,IL-4and LPS).The morphology and phenotypes of these cells were analyzed by flow cytometry and microscopy in12day's culture.Cells on the fourth day were incubated with crude extracts from lesions of condyloma acuminata,foreskin proteins,and PBS,respectively,followed by phenotypic analysis after9-12days' culture.Results Expression of antigens CD1a,CD80,CD86,MHC-I,MHC-II,CD14,CD54was detected after12days' cul-ture.It was shown that MNCs could be induced to differentiate to mature dendritic cells in our culture system.After incubation with crude extracts from lesions of condyloma acuminata for another9-12days,expression of CD86and HLA-DR was increased on dendritic cells.Conclusions Compared to foreskin and PBS pulsed dendritic cells,expression of CD86and HLA-DR is upregulated on dentritic cells after pulsing with condyloma acuminata lesion proteins.The data suggest that crude extracts from lesions of condyloma acuminata might enhance antigen-pre-senting capacity of dendritic cells and strengthen activation of T lymphocytes.

Objective To evaluate the clinical significance of CK20 mRNA expression in the blood of gastric cancer patients. Methods Expression of CK20 mRNA was detected by FQ-PCR in preoperative peripheral blood, tumour drainage blood and postoperative peripheral blood in 55 gastric patients and 60 blood donors.Results There was no positive expression of CK20 mRNA of peripheral blood in 60 healthy blood donors.The expression rate of CK20 mRNA was 45.45% in preoperative peripheral blood.The expression rate was higher in stage Ⅲ Ⅳ than stage ⅠⅡ patients ( P