AIM: To investigate antitumor immune response induced by Dendritic cells (DCs) pulsed with antigen MAGE-3. METHODS: DCs were obtained from peripheral blood mononuclear cells induced with granulocyte/macrophage colony-stimulating factor (GM-CSF), interleukin-4 (IL-4), tumor necrosis factor-?(TNF-?), and pulsed with antigen MAGE-3. After cocultured with DCs for 7 days, T cells were collected and special CTLs were studied. RESULTS: T cells cocultured with MAGE-3 antigen pulsed DCs effectively killed the MAGE-3 positive MKN-45 tumor cells in vitro . CONCLUSION: DCs pulsed with MAGE-3 are able to present antigen effectively, activate antigen-specific CTLs and induce special antitumor immune response in vitro.

Objective To analyze the epidemiological characteristics of the severe cases of hand , foot and mouth disease ( HFMD) in Danzhou and to provide a scientific evidence for the prevention of severe HFMD.Methods Descriptive epidemiological analysis was used to analyze the characteristics of severe ca -ses of HFMD occurred from 2010 to 2014 .Results A total of 18 960 cases of HFMD were reported in Danzhou City from 2010 to 2014.The death rate (annual deaths/1000 persons) was 0.13%.One hundred and eighty-eight cases (0.99%) were diagnosed as severe HFMD with a male to female ratio of 2.4 :1 and 96.28%of them were scattered inhabiting children .Six cases were died from severe HFMD and all of them were under 2 years of age.In total 87.77%of the severe cases were occurred in children under 2 years of age.The severe cases were mainly occurred in June and July .Children form the countryside showed higher rates of severe HFMD than those from cities and towns .No significant differences in the time between the in-itial diagnosis and treatment for children with HFMD in countryside and urban areas were found .However , the differences in the duration from initial diagnosis to severe HFMD between children in countryside and ur -ban areas were statistically significant (M-W test, P<0.05).Among the 188 severe cases, 82.44% were initially diagnosed as common HFMD cases by the county-level medical institutions and 90.96%were diag-nosed as severe cases by the municipal and above medical institutions .The cases positive for EV71 strains accounted for 44.15%.Conclusion Most of the severe HFMD were developed in scattered inhabiting chil-dren under 2 years of age in the countryside of Danzhou during 2010 to 2014.In order to decrease the mor-bidity and mortality of severe HFMD in children , it is necessary to implement health education for residents , to improve the professional skills of medical staffs in the early diagnosis of HFMD , and to strengthen etiologi-cal surveillances and warning system for HFMD .