Objective To investigate the mechanism of the toxic effect of N-methyl-N-nitrosourea (MNU) on photoreceptor cell apoptosis of rat′s retina. Methods Thirty 50-day-old female Sprague-Dawley ( SD ) rats were intraperitoneally injected with MNU (60 mg/kg) and were put to death by dislocation of cervical vertebra 12, 24, 48, and 72 hours and 7 days after the injection, respectively. The photoreceptor cell apoptosis was detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and transmission electron microscope. The expression of proliferating cell nuclear antigen (PCNA), vimentin and glial fibrillary acidic protein (GFAP) was detected at different time after injection by immunohistochemical methods. Results The apoptotic index of the retina in the posterior pole was (33.6?2.3)%, (46.5?5.7)%, (20.1?5.3)%, (8.2?3.6)% and (2.5?1.3% at the 12th, 24 th, 48 th, and 72nd hour and on the 7th day, respectively, after injection. Karyopyknosis was found in most photoreceptor cells 24 hours after injection. The expression of PCNA was found in internal granular layer and between internal granular layer and choroid 24 hours after injection, reached the peak after 72 hours, and reduced obviously after 7 days. The positive expression of GFAP and vimentin was found in internal and external granular layer 24 hours after injection, reached the peak after 72 hours, and reduced obviously after 7 days. Conclusion MNU may selectively lead the photoreceptor cell apoptosis and proliferation of M?ller cells.

Objective To study the expression of high mobility group box chromosomal protein 1(HMGB-1) in the skin lesions of patients with lupus erythematosus and investigate the role of HMGB-1 in the pathogenesis of lupus erythematosus. Methods Immunohistochemical assay and Western-blot were used to test the expression of HMGB-1 in skin lesions from 20 discoid lupus erythematosus (DLE) patients, 25 systemic lupus erythematosus (SLE) patients and 20 healthy controls. Results In healthy controls, H MGB-1 was mainly expressed in the nucleus of keratinocytes. In skin lesions of DLE patients, HMGB-1 was mainly expressed in mononuclear cells of dermis, but the percentage of positive keratinocytes in epidermis of lesion area was decreased than that of healthy controls (t=11.315, P<0.01). In skins that were not involved,HMGB-1 was also expressed in the nucleus of keratinocytes and there was no difference in the percentage of positive keratinocytes between DLE and healthy controls (P>0.05). By Western-blot, there was no stati-stical significance in total protein between DLE and healthy controls (t=0.681, P>0.05). In SLE, besides the mononuclear cells, HMGB-1 could be detected both in the cytoplasmic and extracellular space in dermis,while the HMGB-1 nuclear expressions in keratinocytes'of epidermis were decreased than those of DLE (t=6.821, P<0.01), and in un-involved skin, HMGB-1 was also expressed in the nucleus of keratinocytes and there was no difference in the percentage of positive keratinocytes with healthy controls (P>0.05). The total protein was increased in SLE than that of healthy controls and DLE patients (t=15.494, P<0.01 ; t=13.221, P<0.01, respectively). The intensity of HMGB-1 was con'elated with SLEDAI and proteinuria (r=0.565, P<0.01,OR=1.027, P<0.05, respectively). Conclusion Compared with healthy controls, there is translocation and alteration of HMGB-1 expression in patients with lupus erythematosus, which indicates that HMGB-1 may be involved in the inflammation of lupus erythematosus.

OBJECTIVE: To explore the curative effect of nasal type extranodal NK/T-cell lymphoma by sequential chemotherapy combined radiotherapy compared with chemtherapy. METHOD: Fifty-seven cases of nasal type extranodal NK/T-cell lymphoma confirmed by pathological morphology and immuno-histochemistry were divided into chemotherapy combined radiotherapy group (34 cases) or chemotherapy group (23 cases). Twenty-three patients in the chemotherapy group alternately applied CHOP, VDLP and MEOP regimen after each two treatments into the clinical observation; Chemotherapy combined radiotherapy group of 34 patients, in addition to the above chemotherapy, applied three-dimensional conformal radiation therapy of the primary site by linear accelerators. Then all of patients were ceased treatment and followed up 3-5 years. RESULT: (1) After treatment, effective rate of two groups was 91.2% and 87.0%, there was no obvious difference (P > 0.05); After follow up about 1 year, effective rate of two groups was 76.5% and 73.9%, there was no obvious difference (P > 0.05); (3) After follow up about 3 years and 5 years, disease free survival (DFS) of two groups was 61.3%, 47.6% and 43.5%, 21.4%, there was obvious difference (P < 0.05). (4) Long-term survival is closely related to treatment mode. (5) B symptoms, advanced (III, IV) stage, the International Prognostic Index (IPI), KPS scores were correlated with prognosis, and were independent prognostic factors. CONCLUSION Treatment with chemotherapy and radiotherapy for nasal type extranodal NK/T-cell lymphoma had obvious curative effect and may improve long-term survival efficiently compared with chemotherapy alone.
Adult ; Antineoplastic Combined Chemotherapy Protocols ; Combined Modality Therapy ; Female ; Humans ; Lymphoma, Extranodal NK-T-Cell ; drug therapy ; radiotherapy ; therapy ; Male ; Middle Aged ; Radiotherapy ; methods ; Treatment Outcome ; Young Adult