Objective To explore the effect of Shengmai injection combined with chemotherapy in treating advanced tumor and its mechanism. Methods 65 patient with malignant tumor, who having the syndrome of yin and qi deficiency, were randomly recruited into a control group and a treatment group. The control group was treated with routine chemotherapy, and the treatment group was treated with routine chemotherapy plus Shengmai injection (60 ml every day). The total effective rate was observed. Results Compared with control group (71.0%), the effective rate of the treatment group (91.2%) showed significant difference (χ~2=4.399, P<0.05). Conclusion The effect of treating malignant tumor with Shengmai injection combined with chemotherapy is good. The therapy can obviously improve symptoms and reduce side effects of chemotherapy.

Objective To study the factors leading to intramedullary pressure (IMP) change during openintramedullynailing (OIMN) of fresh femoral fractures and the relation between fat intravasation and IMP. MethodFifty patients with femoral fractures were submitted to OIMN in early stage after injury. Before operation, a hollow probe was screwed into the medullary cavity and linked with a monitor to record the dynamic pressure change during the OIMN process. The patients were divided into 2 groups according to their IMP. 5ml blood was taken from femoral vein of the affected limb individually before operation and just after fixation of the intramedullary nail. The blood clots were made into frozen sections and stained by oil red-O to find the free fat.The positive rate of free fat in pathological detection between patients with increased IMP and those without were compared.Result(1) Increased IMP could be recorded in some patients with "piston effect" resulted from a certain condition during OIMN. (2) For patients with increased IMP, the positive rate of free fat through pathological detection during the operation was significantly higher than that of before (p(o. ol),but that of patients without increased IMP did not change significantly. The positive rates of the two groups did not have significant difference than those of before operation. During operation, the positive rate of patients with increased IMP was significant higher than that of patients without pressure imcreasing(P

Well-designed operation and management system of research laboratories is essential for scientific innovations and talent training.The reform of the traditional laboratory management to encourage science innovation and development is key to promoting the laboratories to develop in scale,level and efficiency.On the basis of profiling traditional lab management and its setbacks,the paper described the management mode and operation mechanism of Capital Medical University.Such mode and mechanism feature lab project Principal Investigation responsible system,and shared platform for large equipments and services.The paper also discussed lab operation and management system for universities at large.

Objective To investigate the expression and its clinical significance of adisintegrin and metalloprotease-17 (ADAM17) in human glioma.Methods The expressions of ADAM17 in human glioma were detected by immunohistochemical staining.The correlation between ADAM17 expression and the malignance of glioma was analyzed.Results Expression of ADAM17 in human gliomas increased.With the malignant degree,ADAM17 expression has also further increased.However,there was a less ADAM17 expression in normal human brain.The positive expression of ADAM17 in low malignant group was 75％ (18/24),of which 1 case were strong positive.The strong positive expression of ADAM17 was the majority (82.1％,23/28) in malignant group.Conclusion The expression of ADAM-17 was higher in human brain gliomas.With malignance degree increased,of the ADAM-17 expression has gradually increased,suggesting that ADAM-17 might play a certain role in the occurrence and development of glioma.

Objective To investigate the relationship of TCM constitution and syndrome elements based on patients with hyperhomocysteinemia. Methods A survey was conducted among 1316 patients with diabetes, coronary disease, hypertension and cerebral infarction. Totally 326 patients with hyperhomocysteinemia were incorporated in diagnostic data and their constitutions were determined. At the same time, syndrome elements identification method for syndrome elements was applied for judgment, and consistency of the constitution and syndrome elements was analyzed. Results The survey showed that prevalence rate of population with hyperhomocysteinemia was 24.77%;there were 6 cases with mild nature, and 320 cases with biased constitution;phlegm dampness, blood stasis, qi deficiency of constitutions 110 cases, 73 cases and 59 cases, respectively;blood stasis, phlegm, qi deficiency, and dampness were the main syndrome elements of hyperhomocysteinemia, of 100 cases, 98 cases, 73 cases and 59 cases, respectively;the consistency of phlegm dampness constitution and syndromes of phlegm was the most obvious (Kappa=0.89, P<0.05). Conclusion Phlegm dampness, blood stasis, and qi deficiency were the most common constitutions of hyperhomocysteinemia;blood stasis, phlegm, qi deficiency and dampness were the main syndrome elements of hyperhomocysteinemia patients. The two results were consistent, and the consistency of phlegm syndrome and phlegm dampness constitution was the most obvious.

he herpes simplex virus type1 (HSV 1) was used for study on tracing neuronal connections of the cerebellum in the rat. The anterior or posterior lobe of the cerebellum in total 23 rats was injected by HSV 1 (HK 2 strain, from Institute of Virology, Chinese Academy of Preventive Medicine) with the titer of 10 -7 in a volume of 10 15?l for each case. Following a postoperative survival period of 1 5 or 10 days, the animals were perfused and their brains and spinal cords were sectioned and stained immunohistochemically by polycolonal antibodies raised in rabbit against HSV 1. Under the LM, the HSV 1 labelled neurons were shown to be a Golgi like appearance, with clearly labelled cell body and dendrites. It showed that the distributions of the labelled neurons in the CNS depended on the postoperative periods and injection sites. 1. After posterior lobe injection, the labelling was limited in the cerebellum, especially in Purkinje cells and the deep nuclei in 1 day of survival time. Two days later, the labelling could be seen in the vestibular, pontive nuclei and inferior olive nucleus. Anterogradely transneuronal labelling in the ventrolateral thalamic nuclus became apparant 3 days after injetions; 2. After anterior lobe injection, the red nucleus, cuneate nucleus, cuniform nucleus and interstitial nucleus of cajal were labelled after 3 days, in addition to the labelling as shown in those cases with injections in the posterior lobe. The results proved that HSV 1 (HK 2 strain) could be transported both retrogradely and anterogradely in CNS, while the transneuronal transport would mainly occur anterogradely. The distances of HSV 1 transport in neuronal pathways would depend upon the postoperative survival times. This indicates that HSV 1 (HK 2 strain) is a powerful tool for demonstrating the chains of synaptically connected neurons in CNS.

Objective\ To prepare a digoxingen labelled cRNA probe for human tyrosine hydroxylase(hTH 2).Methods\ Using molecular cloning techniques the recombinant plasmid pGEMTH 2 was constructed.According to analysis of restriction endonucleases,the pGEMTH 2 contained the DNA fragment of hTH 2 gene and inserting sit preparation was correct for cRNA probe.From recombinant plasmid pGEMTH 2 prepared cRNA probe was identified by dot blot hybridization.Results\ The dot blot hybridization showed that the positive reaction sites were purple blue.Conclusion\ This probe prepared in present study was sensitive and reliable.This probe might provide a tool for identifying the effect of gene therapy in animal model of Parkinson disease.

Objective To analyze the distribution characteristics and risk factors of extended stay over 30 days at tertiary comprehensive hospitals, and explore methods of shortening average length of stay(LOS).Methods Homepage of all inpatients discharged from October 2014 to October 2015 of 31 tertiary comprehensive hospitals in Shanxi province were collected.39 234 cases of these inpatients stayed over 30 days and their case information was complete.A generalized linear model was used for risk factors analysis.Results With the extension of LOS, most of the inpatients with extended stay were found during their 30th-40th days, accounting for 50.21% of all and constituting the highest daily costs as well.The ratio of inpatients decreases with their LOS, and its distribution presents a positive skew in entirety.Distribution of inpatients over 30 days identified urban employees under medical insurance accounting for the highest ratio among all payment categories,and those from the department of orthopedics for the highest ratio among all departments, while those of diseases from injury and poisoning accounting for the highest ratio among all disease categories.The findings indicated ten important factors for extended stay, namely gender, medical payment category, ICD, inter-department transfer, nosocomial infection, and clinical pathway.Conclusions To strengthen management of specific departments and inpatients, focus on key process of medical quality, and accelerate medical insurance payment reform can effectively control extended stay.

Objective To investigate the protection effect of cyclosporine A on spatial memory following chronic cerebral hypoperfusion in rats and its possible mechanism.Me,ods Sixty SD rats were randomly divided into sham operation,vehicle,low-dose cyclosporine A,medium-dose cyclosporine A,and high-dose cyclosporine A groups.A chronic cerebral hypoperfusion model was prepared by permanent bilateral ligation of bilateral common carotid arteries.From 46 days after modeling,olive oil 1 ml/d was used for intragastric administration in the sham-operation group and the vehicle group.Cyclosporine A 3 mg/kg,6 mg/kg,and 12 mg/kg were administrated intragastrically in the low-dose,medium-dose and high-dose cyclosporine A groups,respectively,once a day for 14 days.The spatial memory was assessed using Morris water maze test.Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of NADPH oxidase 4 (NOX4) mRNA in the cerebral cortex.Immunohistochemical staining and Western blot were used to detect the expression of NOX4 protein in the cerebral cortex.Results The Morris water maze test showed that the escape latencies in all cyclosporine A groups were significantly shorter than the vehicle group (all P ＜0.05).Immunohistochemical staining showed that the percentages of the NOX4-positive cells in the sham-operation,vehicle,low-dose,medium-dose,and high-dose cyclosporine A groups were4.43％ ±0.37％,37.44％ ±4.76％,18.05％ ±2.91％,12.51％ ±3.4％,and 11.06％ ±1.74％,respectively (F =262.021,P ＜ 0.001),the vehicle group was significantly higher than the sham-operation group (P ＜ 0.01),and all cyclosporine A groups were significantly less than the vehicle group (all P ＜ 0.01).RT-PCR showed that the expression levels of NOX4 mRNA in cerebral cortex in the sham-operation,vehicle,low-dose,medium-dose,and high-dose cyclosporine A groups were 0.36 ± 0.03,1.04 ± 0.04,0.58 ± 0.02,0.49 ± 0.01,and 0.40 ± 0.02,respectively (F =1 324.941,P ＜ 0.001),all cyclosporine A groups were significantly lower than the vehicle group (all P ＜ 0.01).Western blot showed that the expression levels of NOX4 protein in cerebral cortex in the sham-operation,vehicle,low-dose,medium-dose,and high-dose cyclosporine A groups were 0.02 ± 0.01,0.27 ± 0.04,0.09 ± 0.02,0.06 ± 0.02,and 0.06 ± 0.01,respectively (F =222.692,P ＜ 0.001),all cyclosporine A groups was significantly lower than the vehicle group (all P＜0.01).Conclusion Cyclosporine A may improve spatial memory following chronic cerebral hypoperfusion in rats by down-regulation of NOX4.

Fourteen adult and immature rabbits were used for the study of laminar scheme of the gray matter in the spinal cord. The spinal sections were cut transversally or sagitally into 80 ?m, 60 ?m, 15?m, and 2?m-thick sections. The sections were stained with toluidine blue or crecyl fast violet for cell body and with Luxol fast blue for myelin sheath. 2?m-thick sections were only stained with pphenylenediamine. In addition, the spinal sections from 2 cases of the animals were treated histochemically for demonstrating AChE activity. According to the Rexed's principle lamination for the cat, we have found that the cytoarchitectonic organization of the rabbit spinal cord was found to be basically similar to that of the cat except for some differences about the extension and structures of the laminae.