Objective To develop autosomal SNP fluorescent-multiplex PCR system,and investigate the allele frequencies of these 13 SNP loci in Southern Liaoning Han population.Methods After selected 13 autosomal SNP loci,SNP fluorescent-multiplex system was developed based on the principle of fragment length discrepant allele specific PCR,and the allele frequencies of these SNP loci in Southern Liaoning Han population were investigated using the established system.Results For the same SNP locus,the homozygote showed a single product peak,and the heterozygote showed two product peaks with different length.For the different SNP loci,the lengths of their PCR products were different.Therefore,we could genotype the 13 SNP loci simultaneously according to the length of products and the amount of product peaks,and the results were in accordance with that of direct sequencing.In the meanwhile,the allelic frequencies of these 13 SNP loci in Southern Liaoning Han population were obtained.Conclusion The SNP fluorescent-multiplex system based on the fragment length discrepant allele specific PCR strategy is simple and economical,and is of a high application value in forensic medicine.

Objective To establish a method for determination of copper, manganese and cadmium in human urine by transversely heated GFAAS.Methods Matrix modifiers were used and background absorption was deducted, copper, manganese and cadmium in human urine were determined by transversely heated GFAAS after digestion.Results 5 g/L Mg(NO3)2 was taken as the matrix modifier when copper and manganese were determined and 0.3 g/L Mg(NO3)2 and 5 g/L NH4H2PO4 was taken as the matrix modifier when cadmium was determined.Under the designed standard conditions, the detection limits of copper, manganese and cadmium were 0.028-0.060 ?g/L;RSDs were 1.1%-5.1%;Recovery rates were 94.2%-107.5%.Conclusion The method is simple, accurate and sensitive, and is applicable to the determination of copper, manganese and cadmium in human urine.

Objective To summarize the experience of laparoscopic operation in the treatment of ovarian cysts. Methods Clinical data of 386 patients with ovarian cysts treated laparoscopically from July 1999 to December 2004 in this hospital were retrospectively analyzed. Results Laparoscopic operation was successfully performed in 384 cases, while a conversion to open surgery was required in 2 cases because of previous operation history or pelvic adhesions. The mean intraoperative blood loss was 20.6 ml (range, 10~60 ml) and the mean operating time, 55 min (range, 20~140 min).The mean postoperative hospital stay was 3 days(range,2~5 days).In a follow-up for 1~60 months (mean, 24 months) in 194 cases, pelvioscopy and B-ultrasonography examinations revealed normal findings. Conclusions Laparoscopic operation for ovarian cysts is an effective and safe method. It is characterized with minimal invasion, quick recovery and short hospitalization.

Objective To explore the relationship of autoantibodies against G protein coupled angiotensin Ⅱ-1 receptor (AT1 R),α1-adrenergic and β1-adrenergic receptors (α1 R and β1 R) with thyrotoxicosis heart disease (THD).Methods The epitopes of the second extracellular loop ofAT1 R (165-191),α1 R (192-218),and β1 R(197-222) were synthesized for screening autoantibodies from 277 participants by ELISA.237 patients with thyrotoxicosis were subdivided into thyrotoxicosis treatment group (n =148) and thyrotoxicosis recovery group (n =89),or into THD group (n =46) and simple hyperthyroidism group (n =191).40 healthy subjects were served as control group.Results (1) The positive rates of autoantibodies against AT1 R,α1 R and β1 R in thyrotoxicosis patients were higher than those in the control subjects (31.6％ vs 12.5％,27.8％ vs 10.0％,and 23.6％ vs 7.5％,all P＜0.05).The positive rates of the three autoantibodies in the patients with Graves' disease were higher compared with thyrotoxicosis caused by other reasons (36.3％ vs 19.7％,32.2％ vs 16.7％,and 28.1％ vs 12.1％,all P＜0.05).(2) In thyrotoxicosis treatment group,the positive rates of autoantibodies against AT1 R and α1 R were higher than those in the hyperthyroidism recovery group (40.5％ vs 16.9％ and 33.1％ vs 19.1％,both P＜0.05).(3) The incidence of autoantibodies against AT1 R and α1 R in THD were significantly higher compared with simple hyperthyroidism (52.2％ vs 26.7％ and 43.5％ vs 24.1％,both P＜0.05).Conclusions Autoantibodies against AT1 R,α1 R,and β1 R may play important roles in the pathogenesis of hyperthyroidism,which may be involved in the progression of THD.

Aim To investigate the effect of plumbagin on invasion and apoptosis of human hepatocellular car-cinoma ( HepG2 ) . Methods HepG2 cells were cul-tured in vitro with different concentrations of plumba-gin, then cell proliferation was observed by MTT as-say; cell invasion was observed by transwell invasion assay; cell apoptosis was detected by flow cytometry, and the protein expression of Bax, Bcl-2 was detected by immunocytochemistry. Results MTT results showed that plumbagin could significantly inhibit cell proliferation compared with the control group, and in a dose-dependent manner ( P <0. 05 ) . Transwell inva-sion assay showed that cell invasion was significantly decreased with increasing concentrations of plumbagin ( P < 0. 01 ) . Flow cytometry showed that apoptosis rate was significantly higher in 4 , 8 μmol · L-1 group of plumbagin compared with that of the control group ( P<0. 01 ) . Immunocytochemistry showed that, with the increasing concentration of plumbagin, Bax protein expression increased, Bcl-2 protein expression was de-creased, both in a dose-dependent manner ( P <0. 01 ) . Conclusion Plumbagin can inhibit HepG2 cell proliferation and accelerate apoptosis of HepG2 cells, but also has the ability to inhibit HepG2 cell in-vasion.
( P < 0. 01 ) . Flow cytometry showed that apoptosis rate was significantly higher in 4 , 8 μmol · L-1 group of plumbagin compared with that of the control group ( P<0. 01 ) . Immunocytochemistry showed that, with the increasing concentration of plumbagin, Bax protein expression increased, Bcl-2 protein expression was de-creased, both in a dose-dependent manner ( P <0. 01 ) . Conclusion Plumbagin can inhibit HepG2 cell proliferation and accelerate apoptosis of HepG2 cells, but also has the ability to inhibit HepG2 cell in-vasion.

Objective To analyze the application value of multislice spiral CT in diagnosis of children malformation of large blood vessels outside the heart.Methods The diagnosis results were compared between echocardiography and multislice spiral CT in 110 children with congenital heart disease,and the children had been confirmed by operation.Congenital heart disease was divided three kinds according to the region:heart,connections between heart and large blood vessels and large blood vessels outside the heart.Results One hundred and ten children with congenital heart disease were selected,and they had 366 malformations.The accuracy rates of echocardiography and multislice spiral CT were 90.7％ (332/366) and 95.6％ (350/366) respectively,and there was no statistical difference (x2 =3.284,P =0.070).In 366 malformations,160 malformations were in heart region.The accuracy rates of echocardiography and multislice spiral CT were 98.1％ (157/160) and 97.5％ (156/160) respectively,and there was no statistical difference (x2 =0.776,P =0.378).There were 55 malformations in connections between heart and large blood vessels.The accuracy rates of echocardiography and multislice spiral CT were 94.5％ (52/55) and 100.0％ (55/55) respectively,and there was no statistical difference (x2 =3.083,P =0.243).There were 151 malformations in large blood vessels outside the heart.The accuracy rates of echocardiography and multislice spiral CT were 81.5％(123/151) and 92.1％ (139/151) respectively,and there was statistical difference (x2 =7.377,P =0.01).Conclusion Multisltce spiral CT has great application value in diagnosis of children congenital heart disease,especially in malformation of large blood vessels outside the heart.

Seat lumbar rotation technique is a commonly used technique,and the clinician is also a kind of clinical curative effect more apparent bone-setting reduction technique.However,the power of spinning technique,direction and position selection is based on the personal experience of the clinician,the lack of a unified,standardized scientific basis for support.Now the article reviews the studies of the rotation of the seat,including lumbar anatomy,biomechanical characteristics,clicks,mechanism of action,and safety.It will provide new ideas for standardizing operations research techniques.

Objective To assess the association of subclinical thyroid dysfunction with fractures. Methods Medline, Embase, Pubmed, Cochrane Library, CBM, CNKI, Wan Fang, and VIP databases were systematically searched from January 1990 to August 2015 to identify prospective cohort studies which have studied the risk of fracture in patients with subclinical thyroid dysfunction. The relative risks ( RR) of cohort studies were pooled respectively, depending on the result of heterogeneity test among the individual studies search. The Stata (version 13. 0) software was used for meta-analysis. Results Nine prospective cohort studies including 292460 participants were identified as eligible for the meta-analysis. RR of subclinical hyperthyroidism for fracture was 1. 39(95%CI 1. 24-1. 55);for hip fracture, RR was 1. 24(95%CI 1. 10-1. 40);for nonspine fracture, RR was 1. 32 (95%CI 1. 09-1. 60). Different gender for subclinical hyperthyroid was associated with higher fracture rates:for females, RR was 1. 15(95%CI 1. 04-1. 27); for males, RR was 1. 31 (95% CI 1. 08-1. 59). The incidence of fracture in patients with subclinical hyperthyroidism was higher during the follow-up. For subclinical hypothyroidism, the RR was 1. 21(95% CI 1. 03-1. 42). Subgroup analysis indicated that there were significant differences between endogenous/exogenous subclinical hyperthyroidism and euthyroid, but no differences between endogenous/exogenous subclinical hypothyroidism and euthyroid were found. Conclusion Subclinical hyperthyroidism is associated with an increased risk of fracture in the population, especially hip fracture and nonspine fracture. During the course of subclinical hyperthyroidism, the incidences of fracture should be noticed both in females and males. However, there is no evidence which could prove a definite association between subclinical hypothyroidism and the risk of fracture.

AIM: To observe the effect of Huangqi Danggui Mixture on diabetic nephropathy. METHODS: Using Huangqi Danggui Mixture to treat 32 patients with diabetic nephropathy,In control group 22 patients were treated with Losartan.The therapeutic course was 3 months for both groups.The changes of clinical symptoms,blood glucose,lipid metabolism and urinary albumin were observed and compared. RESULTS: The total effective rate in the treated group was 80.9% while in the control group 63.3%.The symptoms,urinary protein and albumin as well as lipid metabolism in the treated group all significantly improved after treatment (P

OBJECTIVE To provide the investigative data for timely changing medical gloves according to the study on the dynamic change of physical property of the gloves during abdominal open procedure.METHODS The gloves used in the abdominal open procedures were collected.The tensile strength,break elongation and the morphological values of three types of the gloves(No.6.5,7 and 7.5) were measured and recorded before and after their using at the time point of 0.0h,1.5h,2.5h,3.5h,4.5h,5.5h and 6.5h during operation.RESULTS The tensile strength decreased significantly(P