Objective:To prepare Pingwei dispersible tablets and establish a method for the determination of atractylodin. Meth-ods:HPLC was performed on a Kromasil C18 column(250 mm × 4. 6 mm,5 μm)using methanol-water(75:25)as the mobile phase at a flow rate of 1 ml·min-1 . The detection wavelength was at 340nm. The column temperature was 30℃ and the injection volume was 10 μl. Results:The optimized formula of the tablets was as follows:the proportion of inner disintegrant microcrystalline cellulose,out-side disintegrant carboxymethyl cellulose sodium and HPMC was 40%,9% and 2%,respectively. The adhesive was 2% HPMC in 70% ethanol,and 1. 5% magnesium stearate was used as the lubricant. The linear range of atractylodin was 0. 100 6-0. 503 0 μg(r=0. 999 9),and the average recovery was 99. 3%(RSD=0. 9%,n=6). Conclusion:The formula and the preparation process of the tablets are reasonable and the content determination method is simple,accurate and specific,which can be used in the quality control of Pingwei dispersible tablets.

Hyponatremia is the most common electrolyte abnormality in hospitalized patients,which could be attributed to many factors.Antidiuretic hormone plays an important role in the development and progression of hyponatremia.It has been reported that estrogen affects antidiuretic hormone level,the number and sensitivity of related receptors,and effects of antidiuretic hormone,resulting in gender difference in occurrence and outcome of hyponatremia.

Objective To evaluate the value of strain rate imaging(SRl) in quantitatively detecting regional right ventricular function in hypertension(HBP) patients with different mode of left ventricle.Methods Thirty-eight patients with HBP were divided into left ventricular nermal (LVN) and left ventricular remodeling(LVR) groups according to left ventricular mass index(LVMI) and relative wall thickness(RWT).Twenty-three controls were performed echocradiography examination.Tissue velocity imagings of all the patients and controls were accepted in apical four chamber views.The indies included:peak systolic velocity(Vs),peak early diastolic velocity(Ve),peak late diastolic velocity(Va) and Ve/Va of triscular annulus,peak systolic strain rate(SRs),peak early diastolic strain rate (SRe),peak late diastolic strain rate(SRa) and SRe/SRa of right ventricular free wall.Results Compared with controls,Ve/Va of LVN group decreased significantly,but there were no difference in Vs and Ve.Compared with LVN group and controls,Ve and Ve/Va of LVR group decreased significantly,but there were no significantly difference in Vs.Compared with controls,Ss,SRe and SRe/SRa of LVN and LVR group decreased significantly,but there was no significantly difference in SRs;compared with LVN group,Ss,SRe and SRe/SRa of LVR group decreased significantly,but there was no significantly difference in SRs.Conclusions The systolic and diastolic function of RV in patients with HBP damaged with or without left ventricular remodeling.SRI can evaluated regional right ventricular funciton in these patients accurately.

Objective To evaluate the right ventricular function in patients with chronic obstructive pulmonary disease (COPD) by strain rate imaging. Methods Echocardiography were performed in 41 patients with COPD and 20 controls. Right ventricular frontal thickness (RVFT), right atrial end diastolic diameter(RAEDd) , right ventricular end diastolic diameter (RVEDd), right ventricular end diastolic area (RVEDa) ,right ventricular end systolic area (RVESa) (fraction of right ventricular area change (RVFA) and E/A of tricuspid valve flow were measured in 4-chamber apical view. Tissue velocity imaging (TVI) were accepted in apical four chamber apical views. The indies included peak systolic strain rate (SRs) ,peak early diastolic strain rate (SRe) ,peak late diastolic strain rate (SRa) and SRe/SRa. Results According the pulmonary pressure, the patients with COPD were divided into 2 groups: group PAH (pulmonary artery hypertension) and group NPAH (non-pulmonary artery hypertension). Compared with controls, RVFT, RAEDd and RVEDd of group PAH increased significantly, RVFA and E/A decreased significantly, the upper indices of group NPAH had no significant difference, SRs, SRe and SRe/ SRa of right ventricular free wall and interventricular septal in group PAH and group NPAH decreased significantly, but there was no significant difference of SRa. Compared with group NPAH,SRs,SRe and SRe/SRa of right ventricular free wall and interventricular septal in group PAH decreased significantly,but there was no significant difference of SRa; there was significant positive correlation between SRs of right ventricular free wall and FEV1 /FVC, DLco/VA in patients with COPD,but there was no significant correlation between SRe of right ventricular free wall and RVFA, E/A,FEV1 /FVC and DLco/VA. Conclusions Function of right ventricle in patients with COPD can be damaged with or without pulmonary hypertention; SRI can detect right ventricular function in these patients easily and noninvasive.

BACKGROUND:Studies have shown that human umbilical cord mesenchymal stem cel s can improve pulmonary ventilation function by reducing inflammations. OBJECTIVE:To observe the therapeutic effect of human umbilical cord mesenchymal stem cel transplantation on acute lung injury. METHODS:Thirty Sprague-Dawley rats were randomized into normal group, model group and experimental group. Rats in the latter two groups were used to establish animal models of acute lung injury by intratracheal instil ation of lipopolysaccharide. One hour after modeling, rats in the experimental group were intratracheal y administered human umbilical cord mesenchymal stem cel suspension (0.1 mL, 1×106 cel s), and those in the other two groups were given normal saline in the same dose intratracheal y. Twenty-four hours after treatment, the pathological changes of lung tissue were observed using hematoxylin-eosin staining;the wet and dry weight ratio of the lung tissue and the levels of serum interleukin-1 and interleukin-8 were detected. RESULTS AND CONCLUSION:Compared with the normal group, the wet and dry weight ratio of the lung tissue and the levels of serum interleukin-1 and interleukin-8 were significantly increased in the model group (P<0.05), while compared with the model group, these levels were significantly decreased in the experimental group (P<0.05). Hematoxylin-eosin staining results showed clear alveolar space structure with complete alveolar septum in the normal group. In the model group, the alveolar septum was markedly thickened, and there was visible pulmonary capil ary hyperemia, edema, as wel as a large amount of inflammatory cel infiltrations in the pulmonary capil aries and alveolar space. Edema fluid rich in proteins was observed in a part of the pulmonary alveoli, and an extensive transparent membrane formed in the alveolar space. In the experimental group, the alveolar structure was clear, but the alveolar septum became thickened, and red blood cel s and a smal amount of infiltrated inflammatory cel s were leaked from the pulmonary interstitial tissue. In conclusion, human umbilical cord mesenchymal stem cel transplantation for treatment of acute lung injury can reduce inflammatory factor levels and al eviate lung injury.

Objective To evaluate the left ventricular mechanical dyssynchrony in patients with chronic heart failure(CHF)by two dimensional speckle tracking imaging(2D-STI).Methods 37 CHF patients were enrolled in this study.According to left ventricular ejection fraction(LVEF),the patienst were classified into two subgroups:group A,LVEF≤ 35 ％;group B,35 ％＜LVEF＜50 ％.35 healthy volunteers constituted the control group(group C).Standard 2D images were acquired in the 2-,3-and 4-apical views as well as the parasternal short-axis views at the level of the mitral valve and papillary muscles.The time to peak-systolic strain and strain rate were measured for each segment in the level of the mitral valve and papillary muscles in Qlab software.Dyssychrony parameters were defined as the standard deviation and the maximal time delay of the time to peak-systolic strain and strain rate for 12 segments.Parameters were compared among the three groups respectively.The correlation between dyssynchrony parameters and LVEF were analysed in CHF patients.The cut-off value of each parameter were derived from group C based on statistical principles.Dyssychrony rate of each parameter were calculated in group A and B,and compared within each group respectively.Results Compared with group C,all the dyssychrony parameters in group A were significant higher(P＜0.05),and part of the parameters(Tsl-12SD,Tsl-12Dif,Tslr-12SD,Tslr-12Dif and Tsr-12SD)in group B were remarkable higher(P＜0.05).Dyssychrony parameters in CHF patients correlated closely with LVEF(P＜0.05).Significant differences among dyssychrony rates were noted in both group A and B(P＜0.05),and Ts1-12SD showed the highest value in both subgroups.Conclusions 2D-STI is useful in quantifying left ventricular systolic dyssychrony in CHF patients,and the longitudinal parameters are more efficient to detect mechanical dyssychrony than the radial parameters and circumferential parameters.

Objective　To explore the effects of treatment and mechanism of sodium salicylate on blast deafness. Methods　Guinea pigs were divided into blast group, blast plus sodium Salicylate group and sodium solicylate group. The animals in the blast group were exposed to blast average peak pressure 176.7 dB(SPL); sodium salicylate was injected IP 400 mg/kg,1/d. Monitoring ABR threshold, the value of MDA of and counting the number of hair cells on the cochlea preparation, SEM for hair cells was observed. Results　The ABR threshold shift in the blast group was 46.75±13.56 dB，38.0±12.4 dB,35.5±10.67 dB，41.5±12.57 dB,33.75±8.42 dB, post-blast 1 d，3 d，1 w，2 w,4 w respectively. And that of the blast plus sodium salicylate was 46.65±11.7 dB，38.25±9.52 dB,30.5±9.17 dB,30.0±9.17 dB,23.0±6.57 dB. The two groups differed significantly at 1 w，2 w，4 w(P＜0.05). The value of MDA differed significantly at 2 w,4 w(P＜0.05). And the hair cells lost also differed significantly. It was observed that sodium salicylated can raise the ABR thresbold temporarily 24 hrs during simple sodium salicylate adminastration. However it recovered 14 hrs after the injection.Conclusion　Sodium salicylate can partially attenuate the hearing loss caused by blast. It is probable that sodium salicylate can clear peroxicative produced during blast and therefore protect hair cells and make hearing loss partially recover.

ObjectiveIn order to investigate the effects of shock wave on atrial natriuretic peptides immunoreactivity in the stria vascularis and hair cells of cochlear and correlation of auditory threshold shift,to study the mechanism of deafness caused by blast trauma. MethodsAfter shock wave, different groups of guinea pigs were studied by ABC immunohistochemistry, image analysis and auditory brainstem response. ResultsANP - IR optic density in experimental group was higher than that of control in the stria vascularis and inner hair cells. The change of ANP- IR optic density was directy correlated with ABR threshold shift (r2 =0.9185. P2＜0.05). There was no change of ABR and ANP - IR optic density on outer hair cells. ConclusionThe change of ANP in the stria vascularis may be a compensatory mechanism to heating lose caused by shock wave; ANP in inner cells may be a pathological manifestation of shock wave. The changes of ANP - IR in outer cells may be a false appearance, it was the shock wave which lead to outer cells ANP running off.

Objective To evaluate the role of procalcitonin (PCT)in the early diagnosis for hand,foot and mouth disease (HFMD)with bacterial infection.Methods Clinical data of 234 HFMD children who were hospitalized be-tween January and July 2012 were analyzed retrospectively,according to discharge diagnosis,data were divided into simple viral infection group (n=178)and viral associated with bacterial infection group (n=56),and data of 20 healthy children were selected as the control group.Serum PCT,C-reactive protein (CRP)and peripheral white blood cell (WBC)count were compared.Results There was significant difference in the level of PCT,CRP and WBC among three groups (F=381.94,24.18,and 26.46,respectively,all P<0.05).The positive rate of PCT,CRP and WBC among three groups was significantly different(χ2=178.25,38.98,and 71.21,all P<0.05),PCT,CRP and WBC in bacterial infection group(92.86%[52/56],85.71%[48/56],and 87.50%[49/56]respectively)were significantly higher than those of simple viral infection group (3.93%[7/178],62.36%[111/178],and 30.90%[55/178]respectively)and healthy control group (5.00%[1/20],10.00%[2/20],and 5.00%[1/20]respectively).The sensitivity rate of PCT,CRP and WBC was 92.86%,85.71%,and 87.50% respectively,specificity rate was 95.00%,90.00%,and 95.00% respectively.Conclusion The level of PCT has important value for the early diagno-sis of HFMD with bacterial infection,and its accuracy rate and sensitivity are better than CRP and WBC levels.

Objective To investigate the effect of rice water on prevention of diarrhea in patients with traumatic head injury undergoing enternal nutrition. Methods 110 patients with traumatic head injury in neurosurgery department were selected and randomly divided into the observation group and the control group.The observation group was fed with rice water in the early two days of entemal nutrition and then begun with nutrient solution on the third day, as to the control group,patients were fed with nutrient solution at the beginning of enternal nutrition. Incidence of diarrhea in these two groups were viewed and underwent χ2 test. Results The number of samples with diarrhea in the observation group(3 cases) was significantly less than that of the control group (12 cases). Conclusions Feeding with rice water can clean the intestine, regulate the function of gastrointestinal tract and reduce the incidence of diarrhea obviously in patients with traumatic head injury during the early period of enternal nutrition.