Objective: To study the changes in the expression of lung resistance protein(LRP) mRNA after multiple hyperthermia and the sensitivity to Adriamycin in HepG2 cell. Methods: HepG2 cell were treated by ten repeated cycles of exposure at 42 degree for 60 minute once a day, and stable HepG2 cell was obtained after hyperthermia. Compared with control HepG2 cell without hyperthermia, Real- time PCR and Western Blot were facilitated to observe the expression of LRP gene, Growth inhibition of Adriamycin to two groups was studied by MTT assay. The sensitivity of cell to Adriamycin was analyzed. Mean fluorescence intensity of ADM were detected by flow cytometry, and the changes of hyperthermia on intracellular concentration of ADM were analyzed. Results: Compared with control HepG2 cell without hyperthermia, the mRNA and protein levels of LRP in HepG2 cell increased 4.01?0.23 and 4.67?0.36 times respectively. This difference was of statistical significance(P

Hepatic resection has been the main form of the treatment of liver benign and malignant tumors. With the popularity of applications of minimally invasive techniques in surgery in recent years, laparoscopic hepatectomy(LH) application has also achieved a rapid development. It' s advantages are known as a small open incision, light systemic response and rapid recovery, meanwhile it can also attain the same efficiency as the open surgery does. However, there also exist disadvantages, such as complicated conduct, expensive instruments, and bleeding problems. This article reviews the process of development of laparoscopic liver resection, and elaborates its current status, existing problems, and evaluation of its treatment and efficiency.

In recent years, neurobehavioral end point was often used in environmental epidemiological researches to assess and characterize the adverse effects of environmental pollutants. The applications of neurobehavioral evaluation not only improve the ability to identify the early, sub clinic adverse effects of environmental pollutants exposure, but also help to screen out the victims for further protection. At present, there are many neurobehavioral evaluation methods have been applied in the environmental epidemiological researches at home and abroad, some methods that are considered applicable in China were reviewed in this article.

Objective To describe the relationship between ambient air pollutants(TSP,SO2,NOx) and the level of children’s lung function (FVC,FEV1,MMEF) in China. Methods The papers on ambient air pollution and children lung function published from 1985 to 2006 were collected and 11 of them were selected according to the following criteria: children aged 7-15; local air quality monitoring results were reported; strict quality control were taken when testing children lung function; the results were expressed by the average of measure. Results The selected papers included the results of 7 cities in China. Among them,the result of 6 cities showed that the levels of children lung function were significantly lower in the heavy air pollution areas compared with that in the light pollution areas. According to the papers,the average levels of ambient air TSP were in the range of 0.084 mg/m3-0.835 mg/m3,SO2(cSO2) were 0.013 mg/m3-0.929 mg/m3,NOx were 0.044 mg/m3-0.229 mg/m3,there was a significant negative correlation between the levels of TSP and SO2 and children FVC and FEV1,as well as the levels of NOx and children MMEF,the correlation coefficients were as follows,-0.797(t=-4.384,P=0.001) between TSP and FVC,-0.693(t=-4.190,P

Lead is a kind of poisonous heavy metal which is widely existed in our environment. It is so harmful for children’s health. The whole society has paid much attention to this problem all the time. Blood lead level is an important index which can measure how seriously children have been exposed to lead. By consulting the papers on the studies of children's blood lead levels,the sources and influencing factors of blood lead levels of children in China were reviewed in the present paper,meanwhile,some suggestions were provided to decrease the lead contact probability and improve children's health.

Objective To describe blood lead levels (BLLs) and the distribution character of children in China as a whole. Methods The research papers on children's BLLs published in 1994-Mar,2004 were collected through Chinese Biomedical Disc(CBMDisc) or other ways, and 32 papers were selected into the present analysis according to the four main criteria: (1) BLLs were measured by graphite atomic absorption spectroscopy (GFAAS) or inductively coupled plasma-mass spectrometry(ICP-MS); (2) high quality guarantee and quality control were reported, standard substance using was necessary; (3)there were no local lead pollution resources in the areas where the involved children lived in; (4)the samples were more than 100. Results The mean BLL of Chinese children was 92.9 ?g/L(37.2-254.2 ?g/L)and 33.8%(9.6%-80.5%)of children, as for individual BLL, were higher than 100 ?g/L. It indicated that BLLs in boys were significantly higher than that in girls(P

Objective To investigate the relationship between microvessel density (MVD), clinicophathological characteristics and the prognosis in brain astrocytoma. Methods To determine the generation level of microvessel in 52 cases brain astrocytoma with immunohistochemistry. Results MVD in brain astrocytoma tissues was higher (71.8?27.8) than that in normal brain tissues (25.8?6.2) (P

Hepatolenticular degeneration,namely Wilson's disease,an autosomal recessive disorder of copper metabolism.The author of this article gave a definition to toxin-pathogen,discussed the basic concept of collaterals disease briefly.And pointed out that hepatolenticular degeneration is a disease due to toxin-pathogen invading collaterals,which was caused by disorder of copper metabolism.This can result in toxin stagnating in collaterals and stagnation of blood stasis in collaterals in the initial stage,and collaterals injured at last.To improve QI-blood circulation and regulate the function of zang fu is important in order to accelerate elimination of toxin and prevent copper from stagnating. Specific therapy vary according to different clinical symptoms,such as promoting blood circulation for removing obstruction in collaterals and clearing toxin, clearing heat-fire and clearing toxin and dispersing stagnated liver-qi for promoting bile flow et al.

Objective To evaluate Chinese rural children's blood lead levels (BLLs) and identify its distribution features and to provide data for policy development to the prevention of rural environmental lead pollution. Methods The papers on rural children's BLLs published from 1994 to Oct, 2008 were collected by using CNKI's (China National Knowledge Infrastructure) Chinese Journal Full-test Database and other ways. The papers which were eligible for the following criteria were reviewed:(1) BLLs measured by atomic absorption spectroscopy (graphite or others) or inductively coupled plasma-mass spectrometry; (2) strict quality control; (3) no local lead pollution sources in the areas where the screened subjects live in; (4) children aged from 0 to 14 years old; (5)sample size more than 40. Results Analysis on the included 32 papers indicated that, the mean BLLs of Chinese rural children between 1994 and 2008 was 74.93 ?g/L ( range:41.14-193.54 ?g/L)and 19.32%( range:2.2%-43%) of the subjects had BLLs higher than 100 ?g/L. The rural children’s BLLs changed from 87.53 ?g/L to 71.16 ?g/L after the use of lead free gasoline in 2000 in China, which were both lower than the general children's BBLs before 2000 and after 2001. The children in Beijing city and Shandong province showed the highest mean BLLs , with 99.16 ?g/L and 92.13 ?g/L respectively; while the children in Jilin province and Hebei province showed the lowest levels, with 41.14 ?g/L and 56.14 ?g/L respectively. The comprehensive analysis of 18 papers indicated that the mean BLLs in rural areas and urban areas were 77.90 ?g/L and 87.24 ?g/L respectively ( u=3.73, P

Objective To estimate the impact of cold wave on daily death of cerebra-cardiovascular diseases in Beijing by using case-crossover design. Methods The data of death counts was provided by Beijing Center for Disease Control and Prevention while the meteorological data was provided by Institute of Urban Meteorology,China Meteorological Administration (CMA),Beijing. The impact of six cold waves from Jan 1,1998 to Jun 30,2000 on daily death of cerebra-cardiovascular diseases were analyzed by using 1∶1 one-directional retrospective design and 1∶2 bi-directional symmetry control design. The cases were the people who dead during winter. Controls were the seventh day before or after the date of cases (or the fourteenth day). Results There were six cold waves in the investigation duration. The third cold wave in which minimum temperature declined obviously with decreasing humidity and increasing air pressure had significantly statistical significance in OR and the lag time of this cold wave was 0 day,the OR values were 1.500 (95%CI:1.032-2.181) for cardiovascular disease (CVD),respectively 1.913 (95%CI:1.066-3.432) for acute myocardial infarction,1.679 (95%CI:1.139-2.474) for cerebra-vascular disease(CBD) respectively. The other five cold waves do not have significant impact on cerebra-cardiovascular diseases. Conclusion Most cold waves studied in Beijing do not impact the daily death counts caused by cerebra-cardiovascular diseases. The cold wave with significant increased air pressure may increase the risk of death of cerebra-cardiovascular diseases without any lag time in Beijing.