Objective: To study the changes in the expression of lung resistance protein(LRP) mRNA after multiple hyperthermia and the sensitivity to Adriamycin in HepG2 cell. Methods: HepG2 cell were treated by ten repeated cycles of exposure at 42 degree for 60 minute once a day, and stable HepG2 cell was obtained after hyperthermia. Compared with control HepG2 cell without hyperthermia, Real- time PCR and Western Blot were facilitated to observe the expression of LRP gene, Growth inhibition of Adriamycin to two groups was studied by MTT assay. The sensitivity of cell to Adriamycin was analyzed. Mean fluorescence intensity of ADM were detected by flow cytometry, and the changes of hyperthermia on intracellular concentration of ADM were analyzed. Results: Compared with control HepG2 cell without hyperthermia, the mRNA and protein levels of LRP in HepG2 cell increased 4.01?0.23 and 4.67?0.36 times respectively. This difference was of statistical significance(P

Objective To investigate the relationship between microvessel density (MVD), clinicophathological characteristics and the prognosis in brain astrocytoma. Methods To determine the generation level of microvessel in 52 cases brain astrocytoma with immunohistochemistry. Results MVD in brain astrocytoma tissues was higher (71.8?27.8) than that in normal brain tissues (25.8?6.2) (P

Objective To describe the relationship between ambient air pollutants(TSP,SO2,NOx) and the level of children’s lung function (FVC,FEV1,MMEF) in China. Methods The papers on ambient air pollution and children lung function published from 1985 to 2006 were collected and 11 of them were selected according to the following criteria: children aged 7-15; local air quality monitoring results were reported; strict quality control were taken when testing children lung function; the results were expressed by the average of measure. Results The selected papers included the results of 7 cities in China. Among them,the result of 6 cities showed that the levels of children lung function were significantly lower in the heavy air pollution areas compared with that in the light pollution areas. According to the papers,the average levels of ambient air TSP were in the range of 0.084 mg/m3-0.835 mg/m3,SO2(cSO2) were 0.013 mg/m3-0.929 mg/m3,NOx were 0.044 mg/m3-0.229 mg/m3,there was a significant negative correlation between the levels of TSP and SO2 and children FVC and FEV1,as well as the levels of NOx and children MMEF,the correlation coefficients were as follows,-0.797(t=-4.384,P=0.001) between TSP and FVC,-0.693(t=-4.190,P

Hepatic resection has been the main form of the treatment of liver benign and malignant tumors. With the popularity of applications of minimally invasive techniques in surgery in recent years, laparoscopic hepatectomy(LH) application has also achieved a rapid development. It' s advantages are known as a small open incision, light systemic response and rapid recovery, meanwhile it can also attain the same efficiency as the open surgery does. However, there also exist disadvantages, such as complicated conduct, expensive instruments, and bleeding problems. This article reviews the process of development of laparoscopic liver resection, and elaborates its current status, existing problems, and evaluation of its treatment and efficiency.

Lead is a kind of poisonous heavy metal which is widely existed in our environment. It is so harmful for children’s health. The whole society has paid much attention to this problem all the time. Blood lead level is an important index which can measure how seriously children have been exposed to lead. By consulting the papers on the studies of children's blood lead levels,the sources and influencing factors of blood lead levels of children in China were reviewed in the present paper,meanwhile,some suggestions were provided to decrease the lead contact probability and improve children's health.

Objective To describe blood lead levels (BLLs) and the distribution character of children in China as a whole. Methods The research papers on children's BLLs published in 1994-Mar,2004 were collected through Chinese Biomedical Disc(CBMDisc) or other ways, and 32 papers were selected into the present analysis according to the four main criteria: (1) BLLs were measured by graphite atomic absorption spectroscopy (GFAAS) or inductively coupled plasma-mass spectrometry(ICP-MS); (2) high quality guarantee and quality control were reported, standard substance using was necessary; (3)there were no local lead pollution resources in the areas where the involved children lived in; (4)the samples were more than 100. Results The mean BLL of Chinese children was 92.9 ?g/L(37.2-254.2 ?g/L)and 33.8%(9.6%-80.5%)of children, as for individual BLL, were higher than 100 ?g/L. It indicated that BLLs in boys were significantly higher than that in girls(P

In recent years, neurobehavioral end point was often used in environmental epidemiological researches to assess and characterize the adverse effects of environmental pollutants. The applications of neurobehavioral evaluation not only improve the ability to identify the early, sub clinic adverse effects of environmental pollutants exposure, but also help to screen out the victims for further protection. At present, there are many neurobehavioral evaluation methods have been applied in the environmental epidemiological researches at home and abroad, some methods that are considered applicable in China were reviewed in this article.

Objective To evaluate Chinese children’s blood lead levels(BLLs) and identify the distribution features with time and to provide the data for developing the policy to control environmental lead pollution.Methods The papers on children BLLs published from 2004 to Aug.2007 with sampling time since 2001 were collected,by searching Chinese Biomedical Disk(CBMDisk),Chinese Journal Full-test Database(CJFD) and other ways.Thirty-five articles eligible for inclusion criteria were reviewed.Also,the data were compared with our former study carried out in 2004 with articles studying time between 1995 and 2003.Results The mean BLL of Chinese children between 2001 and 2007 was 80.7 ?g/L(45.5-165.3 ?g/L),and 23.9%(3.2%-80.7%)were higher than 100 ?g/L,both of which were lower than the levels in 1995 to 2003.Four of 24 provinces or cities reported had average BLLs≥100 ?g/L.Of the 24 provinces or cities,only four had higher BLL and prevalence rates of lead poisoning(BLL ≥100 ?g/L) than that in 1995 to 2003.The mean BLL of children living in industrial areas was higher than that of children in urban and suburbs areas,and the mean BLL in suburbs was higher compared with urban areas.Boys BLL was 79.3 ?g/L,significantly higher than girls(76.9 ?g/L)(P

Objective To analyze the relationship between children's respiratory health and ambient air pollution in China.Methods The studies on ambient air pollution and children's respiratory health in China published from 1980 to 2008 were collected and 12 of them were selected.Pearson correlation and linear regression analysis were used to find the correlations between levels of air pollutants and children's history prevalence of respiratory diseases and symptoms.Results Strong associations between the levels of TSP and the history prevalence of cough, long-term cough, sputum, long-term sputum, bronchitis for children were found, and 0.50%,0.12%,0.43%,0.09% and 0.51% increased respectively as per 10 ?g/m3 increased for TSP;there were significant associations between the levels of PM10 and the history prevalence of cough, sputum and bronchitis for children, and 2.64%, 2.27%,2.17% increased respectively as per 10 ?g/m3 increased for PM10.Significant associations were also found between the levels of PM2.5 and the history prevalence of cough, sputum and bronchitis for children, and 4.56%,3.49%, 3.74% increased respectively as per 10 ?g/m3 increased for PM2.5.Pearson correlation analysis indicated a significant increase of the history prevalence of wheeze for children with increase of levels of SO2;there were significant associations between the increase of the levels of SO2 and increase of the history prevalence of cough and sputum for children as the levels of SO2 lower than 0.15 mg/m3 , and 1.65% and 1.50% increased respectively as per 10 ?g/m3 increase of SO2.Significant associations between the levels of NOx lower than 0.10 mg/m3 and the history prevalence of long-term cough, long-term sputum, bronchitis for children were found, and 0.86%, 0.51% and 3.21% increased respectively as per 10 ?g/m3 increase of NOx.Furthermore, the associations between air pollutants and the history prevalence of children's respiratory health in north China were more significant.Conclusion The air pollutants in China are risk factors of children's respiratory system health, and impacts of air pollutants on children's respiratory system health in north China is more significant than that of the whole regions.

Objective To evaluate Chinese rural children's blood lead levels (BLLs) and identify its distribution features and to provide data for policy development to the prevention of rural environmental lead pollution. Methods The papers on rural children's BLLs published from 1994 to Oct, 2008 were collected by using CNKI's (China National Knowledge Infrastructure) Chinese Journal Full-test Database and other ways. The papers which were eligible for the following criteria were reviewed:(1) BLLs measured by atomic absorption spectroscopy (graphite or others) or inductively coupled plasma-mass spectrometry; (2) strict quality control; (3) no local lead pollution sources in the areas where the screened subjects live in; (4) children aged from 0 to 14 years old; (5)sample size more than 40. Results Analysis on the included 32 papers indicated that, the mean BLLs of Chinese rural children between 1994 and 2008 was 74.93 ?g/L ( range:41.14-193.54 ?g/L)and 19.32%( range:2.2%-43%) of the subjects had BLLs higher than 100 ?g/L. The rural children’s BLLs changed from 87.53 ?g/L to 71.16 ?g/L after the use of lead free gasoline in 2000 in China, which were both lower than the general children's BBLs before 2000 and after 2001. The children in Beijing city and Shandong province showed the highest mean BLLs , with 99.16 ?g/L and 92.13 ?g/L respectively; while the children in Jilin province and Hebei province showed the lowest levels, with 41.14 ?g/L and 56.14 ?g/L respectively. The comprehensive analysis of 18 papers indicated that the mean BLLs in rural areas and urban areas were 77.90 ?g/L and 87.24 ?g/L respectively ( u=3.73, P