Objective To evaluate the role of miR219‐2‐3p in gastric cancer and the underlying mechanisms .Methods Real time RT‐PCR was employed to quantify the expression level of miR219‐2‐3p in matched tissues of 82 patients .In vitro cell proliferation , the expression of tumorgenesis related protein ERK1/2 was performed by overexpressing miR‐219‐2‐3p in gastric cancer cell line MGC803 .Results The expression level of miR‐219‐2‐3p significantly decreased in late stage gastric cancer (P< 0 .05) .The cell proliferation rate was significantly reduced in gastric cancer cell lines overexpressing miR‐219‐2‐3p .Furthermore ,the expression level of p‐ERK that activated form of ERK was significantly decreased whereas the total level of ERK was unchanged after overex‐pressing of miR‐219‐2‐3p in MGC803 cell lines .The expression level of p‐ERK was also increased in gastric cancer tissues .Conclu‐sion miR‐219‐2‐3p may acts as a tumor suppressor in gastric cancer by decreasing the activity of ERK 1/2 signaling pathway .

Objective To observe the effect of calcitonin on senile osteoporosis. Methods Eighty-six osteoporotic patients diagnosed by double-energy X-ray absorptiometry and manifested clinical symptoms and with spinal bone mineral density (BMD) 2 or more were divided into two groups: 46 patients in the observed group were administered 100 IU calcitonin once a day for three days, and changed to 50IU calcitonin once every other day for one month; repeat the administeration method after one month rest and plus oral calcium carbonate vitamin D 600 mg every day for a period of 6 months; another 40 patients in the control group only took calcium carbonate vitamin D 600 mg every day for 6 months. The BMD of L1-4 and hips was examined before and after treatment. Results Bone pain relieving was more obviously in the observed group than that in the control group. BMD of the lumbar spine in the observed group showed remarkable change after treatment(P

This article describes the physical, technical, and dosimetric aspects of total body irradiation (TBI) that was carried out by using 6MV X-Ray from Varian 2300 C/D Linear Accelerator at a distance of 450 cm from target to the treatment table and at a gantry angle of 270°.The dose to lung tissue was limit by setting the individual lead compensators customized before, and using DPD-510 to monitor the absorbed dose of the reference point the absorbed dose in depth of half of body will be (Din+Dout)/2 after taking treatment in both AP position and PA position.

Objective To establish the platelet antigen panel cells and to apply them in the detection and identification of platelet alloantibody.Methods Human platelet antigen(HPA)1-16 genotyping from 1 500 un-related blood donors in Nanning area were performed by the polymerase chain reaction-sequence specific primers(PCR-SSP)technique,platelet antigen cells with O blood type were chosen to establish the panel cells of platelet antigen.The phenotype of the panel cells were verified by the reference sera from the 14th platelet immunology workshop of the International Society of Blood Transfusion(ISBT).And then these panel cells were used in clinic to detect the platelet alloantibody and the samples from the 14th and 15th platelet immunology workshop of ISBT.Re-sults Six platelet cells with consistent phenotype and genotypes and covering the HPA 1-5 and 1 5 systems were selected to estab-lish the platelet panel cells and successfully applied them in the clinical and scientific sample detection and identification.Conclusion Platelet antigen panel cells are established successfully,which provides the experimental basis for the diagnosis and research of platelet allogenic abnormal immunity diseases.

0.05). The blood glucose, glycosylated hemoglobin and oxidized low density lipoprotein degrade, insulin resistance were improved in probucol group after treatment, while the adiponectin level was increased(P

Objective To conduct eugenics inspections for preparing pregnant couples,to find out risk factors causing fetal birth defects,to provide the scientific basis of eugenic and superior nurture.Methods A total of 2 1 63 preparing pregnant couples were conducted eugenics detection before childbirth to assess pregnant risk.Results A total of 1 547 cases were checked out with preg-nancy risk with the positive rate as 35.76%(1 547/4 326).Among them,the positive rate of male was 26.81% (580/2 1 63).the positive rate of women was 44.71% (967/2 1 63).Women positive rate was significantly higher than the male.Conclusion The eu-genics detection before pregnant could found risk of pregnancy as soon as possible,in order to take corresponding preventive meas-ures in time,has important significance for prevention of birth defects.

Objective:To investigate the mRNA expression of the WIF-1 gene and the methylation of its promoter in breast can-cer, and to determine the correlation between the epigenetic aberrant WIF-1 DNA methylation and the clinicopathological significance of WIF-1 in breast cancer . Methods:RT-PCR and sensitive methylation-specific-PCR (MSP) were used to detect WIF-1 mRNA ex-pression and the methylation of the WIF-1 promoter in 30 breast cancer samples as well as in tumor-adjacent tissue samples and 9 be-nign breast tissues. Results:The WIF-1 mRNA expression in 30 breast cancer samples significantly decreased compared with those of the other two groups. In addition, WIF-1 methylation was more frequent in breast-tumor tissues compared with those in tumor-free tis-sues. Meanwhile, WIF-1 mRNA expression in breast cancer tissues involved the abnormal methylation of its promoter. Clinicopatholog-ical correlation analysis showed that the abnormal methylation of the WIF-1 gene promoter was not associated with age, TNM stage, histotype, or lymph node metastasis. Conclusion:WIF-1 mRNA expression loss due to abnormal methylation may be a crucial factor in breast cancer development and can thus be used in the prognosis and progression of the disease.

Objective To discuss the clinical features and related factors for the patients of lupus nephritis (LN) com-plicated with infection, and summarize relevant nursing intervention. Methods A retrospective analysis of clinical data for 231 hospitalized LN patients from June 2011 to June 2012 was completed and analyzed clinical features and related factors of 35 patients complicated with infection. Results The infection rate of LN patients was 15.2%. The most com-mon infections position was respiratory tract, followed by skin soft tissue and urinary system. Bacteria, viruses and fun-gi was the major pathogenic bacteria. The infection was associated with pathology classification, therapy and activity index. Conclusion LN patients are high-risk groups of infection, and strengthen the concept of sterile, strengthen en-vironmental management and health education can reduce the infection of LN patients in or out hospital.

Objective To study the characteristics of bone marrow hematopoietic recovery such as absolute neutrophil count (ANC) and platelet (PLT ) after accepting hematopoietic stem cell transplantation in the patients with inv (9) .Methods A total of 39589 cases of definitely diagnosed hematonosis in our hospital from January 2010 to October 2015 served as the research subjects . The R banding technique ,polymerase chain reaction(PCR) and flow cytometry instrument were adopted to check chromosome karyotype ,fusion gene and bone marrow hematopoietic recovery related indicators .Results PML-RARα,BCR-ABL1 ,AML-ETO , EVI1 ,CBFβ-MYH11 ,MLL-AF6 ,AML-AF4 ,SET-NUM214 ,SIL-TALI ,IgH rearrangement ,TCR rearrangement and BCL1-IgH and other fusion gene were detected in the patients with inv (9) hematonosis .The recovery situation after receiving hematopoietic stem cell transplantation in the patients with inv (9):ANC recovered to ＞0 .5 × 109/L on 12 d after transplantation ,PLT recovered to ＞20 × 109/L on 16 d after transplantation .The recovery situation after receiving hematopoietic stem cell transplantation in the patients with noninv(9):ANC recovered to ＞0 .5 × 109/L on 12 d after transplantation ,and PLT recovered to ＞20 × 109/L on 13 d after transplantation .Conclusion The time achieving ANC recovery ＞0 .5 × 109/L after hematopoietic stem cell transplantation in the patients with inv(9) and without inv(9) is almost similar ,while the time achieving PLT count recovery in the patients with inv(9) is slightly longer than that in the patients without inv(9) .

In this study, pregabalin controlled porosity osmotic pump tablets which are taken once a day were prepared. Single-factor tests were carried out to investigate the influence of excipients and manufacturing process. The formulation was optimized through orthogonal experiment on three levels of three significant factors including the amount of sodium citrate, and polyethylene glycol 400 and coating weight gain. On the basis of the results of the single-factor tests and the orthogonal experiment, optimal formulation and manufacturing process were obtained. The final tablet formulation contained pregabalin(82. 5 mg), microcrystalline cellulose(40%), sodium citrate(27. 5%), magnesium stearate(0. 5%)and 5% povidone K30 solution as the tablet binder; the coating formulation consisted of cellulose acetrate and 60% of polyethylene glycol 400 as a porogen; the coating weight gain was 3%. In vitro drug release kinetic study suggested that the drug release from controlled porosity osmotic pump tablets was mainly driven by osmotic pressure, which was barely affected by the pH of the release medium. The drug release behavior of the tablets within 12 hours complied with zero-order release rule and the linear correlation coefficient was 0. 991 6. The obtained porosity osmotic pump tablets could effectively slow the drug release rate, reduce concentration fluctuation and improve the safety and convenience for the patients, hence with broad prospects.