Objectives To study the reliability,validity and feasibility of the Chinese version of memory alteration test(M@T).Methods Cross-sectional survey with a convenience sample was employed to interview 220 elderly people over 60 years old,39 patients with mild cognitive impairment(MCI),20 with Alzheimer's disease(AD),and 161 normal cognitive elderly.The survey was,then evaluated with internal consistency,content validity,criterion validity,principal component/factor analysis and influencing factors.Results A Cronbach's α coefficient of 0.818 was obtained in M@T. The correlation coefficients which were the score of the subtest and the total were 0.5-0.9.The correlation coefficient of the scores of the Mini.mental State Examination(MMSE)and the M@T Was 0.933.The 5 factors were extracted with the factor analysis,which could explain the total variance of 69.449%,and the corresponding factors of the proieets have a satisfied amount of factor loading(≥0.4).There were significant diffeFences in the score of M@T among the different cognitive level groups with good discriminant validity(cognitive normal group:39.0±3.7,MCI group:29.0±3.7,AD group:16.9±3.7;F=498.419,P<0.05).There were no significant differences in the score of M@T among the different gender,age,occupation and education level groups.Conclusions The Chinese version of M@T has good reliability and validity and feasibility.The score of the M@T is not affected by gender,age,occupation,education level and other factors.

Objective To observe the effect of sequential therapy of butylphthalide injection and soft capsules for acute cerebral infarction and its influence on plasma lipoprotein phospholipase A2.Methods 120 patients with cerebral infarction were selected in the study,and they were divided into observation group (64 cases) and control group(56 cases) according to the digital table.The control group received conventional therapy plus placebo,the observation group received the sequential administration of butylphthalide injection and butylphthalide soft capsule treatment based on the conventional treatment.Before and after treatment,the plasma lipoprotein phospholipase A2 of the two groups was detected,and the National Institutes of Health Stroke Scale (NIHSS) was evaluated,compared neurological deficit improvement between the two groups and recorded adverse drug reactions of the two groups.Results There were no significant differences in NIHSS score and plasma lipoprotein phospholipase A2 level between the two groups before treatment(all P ＞ 0.05).After treatment,the NIHSS score of neurological impairment,plasma lipoprotein phospholipase A2 level in the observation group were (6.40 ± 5.22) points,(203.26 ± 29.33) ng/mL,those in the control group were (8.59 ± 6.22) points,(253.10 ± 52.99) ng/mL,the differences were statistically significant(t =-1.36,-2.089,P =0.039,0.000).The total effective rate of the observation group was 90.6％,which was higher than 67.9％ of the control group,the difference was statistically significant (x2 =9.676,P =0.002).The incidence rate of adverse reactions was similar in the two groups (P ＞ 0.05).Conclusion Sequential therapy of butylphthalide for acute cerebral infarction can improve the neurological function,decrease the level of plasma lipoprotein phospholipase A2,inhibit the inflammation of blood vessels,improve the prognosis of patients.

Objective To study the influence of Nictinamide on the endotheline(ET-1)in patienta with pulmonary arterial hypertension(PAH).Methods 66 cases of PAH were divided randomly into two groups (n=33for each):the Nictinamide treatment group and the control group.The changes of endotheline were compared before and after treatment.Results After treatment serum endotheline was significantly lower in the treatment group(P＜0.01),but there was no difference in the control group before and aftertreatment(P＞0.05).The significant difference was observed between treatment group and control group(P＜0.01).Conclusion Nictinamide will reduce the level of endothellne and confers some degree therapeutic and preventive value for PAH.

For the purpose to seek the active components in the aerial parts of Verbena officinalis L.with anti-early pregnancy activity, the methanolic extract of this drug was repeatedly chromatographed onsilica gel column. Three iridoid glucosides were isolated and identified on the basis of physical, chemicalevidences and spectroscopic analysis (UV, IR, 1HNMR, 13CNMR, 1H-1HCOSY, 1H-13CCOSY and DEPT)as verbenalin (Ⅰ), 3, 4-dihydroverbenalin (Ⅲ) and hastatoside (Ⅲ).Ⅱ is a new natural compound.

Objective To evaluate the significance of dual-color interphase fluorescence in situ hybridization (FISH) in the engraftment estimation and minimal residual disease (MRD) monitoring after sex-mismatched allogeneic hematopoietic stem cell transplantation (allo-HSCT).Method The fluorescence signal of samples from 35 cases in different periods after sex-mismatched allo-HSCT were detected by interphase FISH using chromosome enumeration probes (CEP) X and Y. Results All of 35 patients had been determined to obtain engraftment after allo-HSCT. When the disease relapsed,FISH showed that the percentage of donor chromosomes was decreased and when the disease got remission,the percentage of donor chromosomes increased. When conventional cytogenetics showed 100 % XX or 100 % XY,FISH showed different percentage of host chromosomes.Conclusions The test of dual-color interphase FISH is reliably sensitive and simple for engraftment evaluation and MRD monitoring post HSCT. It is a good complement method to cell morphology and traditional karyotype analysis.

With development of bio-technique, more and more proteins were applied as clinical approaches. However, the protein homogeneity, especially the N-glycosylation limited the further research and application of these protein drugs. The analysis method for N-glycans is believed to be critical in protein drugs development. To enhance the N-glycans isolation efficiency and accelerate the pretreatment, a new strategy was built on ultrafiltration-devices. New methods increased the isolation efficiency of N-glycans containing N-acetylglucosa mine with 10%-20%. The degrading of N-glycans containing sialic acids was also minimized with this method. 20%-100% more N-glycans with sialic acids were isolated. The pretreatment was finished within 30 min. Coupled with HPLC-HRMS, an effective and reliable strategy designed for protein drugs N-glycans analysis were developed.

Objective To investigate effect of the biological prosthesis on load properties of the proximal femur after hemiarthroplasty with partial resection of the femoral head in order to assess stability of the prosthetic implant.Methods Twenty-four young fresh normal femur (with preservation of femoral head) specimens were assigned to prosthesis group (n =8),control group (n =8) and fatigue group (n =8).Specimens in prosthesis group underwent artificial femoral head replacement using self-designed prostheses.Stress at the femoral head with the model standing on one leg was performed.Biomechanical machine was used to detect the difference of load-stress and load-displacement before and after femoral head replacement under the load of 2000 N.Detection points included lateral superior base of femoral neck (A),medial side of lesser trochanter of femur (B),and inferior side of greater trochanter of femur (C).Results At the load of 2 000 N,the load-stress at inferior side of greater trochanter of femur and medial side of lesser trochanter of femur revealed no significance differences between the normal femur and femur with artificial femoral head replacement (P ＞ 0.05),while the difference was significant at lateral superior base of femoral neck (P ＜ 0.05).There were no significant differences of load-stress at A,B,and C points among fatigue group,prosthesis group and control group (P ＞ 0.05).Conclusion After partial femoral head replacement,the biological prosthesis is effective in maintenance of the normal stress transfer in the proximal femur.

Sphingolipids, especially ceramide and S1P, are structural components of biological membranes and bioactive molecules which participate in diverse cellular activities such as cell division, differentiation, gene expression and apoptosis. Emerging evidence demonstrates the role of sphingolipids in hepatocellular death, which contributes to the progression of several liver diseases including ischaemia-reperfusion liver injury, steatohepatitis or hepatocarcinogenesis. Furthermore, some data indicate that the accumulation of some sphingolipids contributes to the hepatic dysfunctions. Hence, understanding of sphingolipid may open up a novel therapeutic avenue to liver diseases. This review focuses on the progress in the sphingolipid metabolic pathway with a focus on hepatic diseases and drugs targeting the sphingolipid pathway.

Fragmented QRS (fQRS) complex drew a large number of research interests recent years.It said to be prevalent in patients with coronary artery disease and myocardial infarction.Researchers also found fQRS was a sign of myocardial scarring,myocardial focal necrosis and substandard perfusion in patients suffered acute myocardial infarction,and the relationship of fQRS with malignant arrhythmia and cardiovascular events in these patients was also investigated lately,patients with fQRS were said to have an unfavorable prognosis.We summarized the definition,pathogenetic mechanism of fQRS and the relationship with ventricular arrhythmia in this article to figure out the cardiovascular event's predictive value of fQRS in patients suffered with acute myocardial infarction.