1.A STUDY OF THE PATTERNS OF THE ARTERIAL DISTRIBUTION OF SPINAL CORD
Baochun JIN ; Jinku LI ; Jingde WANG
Acta Anatomica Sinica 1953;0(01):-
The arteries of the spinal cord on surface have been observed in 62 Chinese adults and 39 children.Anteriorly, the spinal cord was divided into 3 areas, according to the arterial supply.The superior cervical area(segments of C_(1~3) or C_(1~4))was mainly supplied by the anterior spinal arteries; the cervical enlargement area (segments of C_4 or C_5 to T_5 or T_6) was mainly supplied by the arteria intumescentia cervicalis and the lumbar enlargement area (below the segments of T_6 or T_7) was mainly supplied by the arteria intumescentia lumbalis. In the intermediate places between these areas (segments of C_(3~4) and T_(5~6)), the anterior median artery was often narrow or interrupted. Usually the anterior spinal artery united with its fellow of the opposite side to form a single trunk (46.53%). The anterior radicular arteries averaged 5.2 (2~10) in number. The arteria intumescentia cervicalis frequently existed at C_7 and C_8 (C_5~T_3). The arteria intumescentia lumbalis mostly located at T_9 (T_6~L_2), and 72.28% of them being on the left side.Posteriorly, the arteries developed well on the two enlargements. The posterior spinal arteries arised frequently from the vertebral artery (76.41%). The posterior radicular arteries averaged 9.3 (5~21) in number.The authors have raised an objection to the view that the segments of T_4 and L_1 of spinal cord are poor supplied with blood.
2.A PARALLEL SWITCH FABRIC BASED ON CROSSBAR
Jinku LI ; Deyun ZHANG ; Lei GAO
Journal of Pharmaceutical Analysis 2005;17(2):122-126
With the increase of link rate, the arbitrator of centralized switch fabric becomes too complicated to implement. A parallel switch fabric based on crossbar, named as PSFBC (Parallel Switch Fabric Based on Crossbar), has been proposed in this paper. PSFBC is composed of k switches whose rate is 1/k of link', these switches exchange cells in parallel; this increases the arbitrator's period and make it easy to implement. Load is evenly distributed to each switch with FCFS (First Come First Serve) rule, it can keep the order of cells in one stream. A multi-class queue scheduling policy is used in PSFBC to ensure the quality of realtime streams. Experiments show that the load on each switch in PSFBC is well balanced, its average delay of cells is little and its performance is very close to centralized switch; and with the increase of number of parallel switches, the loss of PSFBC's performance keeps very small, it becomes easier to implement.
3.Expression of S100A7 in basal-like breast carcinoma and its clinical significance
Li JIANG ; Wenming ZHAO ; Jirui SUN ; Bingjuan ZHOU ; Jinku ZHANG
Journal of International Oncology 2014;41(3):229-232
Objective To detect the protein expressions of S100A7 and CD34 in basal-like breast carcinoma and analyze the clinical relationship between S100A7 and basal-like breast cancer and the connection with CD34 markers microvessel denisity (MVD).To study the function of S100A7 in basal-like breast cancer angiogenesis,invasion and metastasis.Methods Immunohistochemistry (IHC) was used to detect the expressions of S100A7 and CD34 in 60 cases of basal-like breast cancer,40 cases of non basal-like breast cancer and 20 cases of normal breast tissues.Results The expressions of S100A7 in basal-like breast cancer,non basallike breast cancer and normal breast tissues were 83.33%,62.50% and 35.00% respectively.There were significant differences among them (x2 =5.556,P < 0.051; x2 =17.107,P < 0.05; x2 =4.051,P < 0.05).There was positive correlation between S100A7 and lymph node metastasis,TNM staging and MVD of basal-like breast cancer patients.But there was no significant correlation between S100A7 and patient's age and tumor sizes.Conclusion The up-regulation of S100A7 expressions may be associated with the occurrence and development,invasion and metastasis,angiogenesis of basal-like breast cancer.The effects of S100A7 promoting angiogenesis may be an important factor in the acceleration of the local invasion and distant metastasis in basallike breast cancer.
4.Expression of Cripto-1 in basal-like breast carcinoma and its clinical significance
Li JIANG ; Hong CHEN ; Wenming ZHAO ; Bingjuan ZHOU ; Jinku ZHANG
Cancer Research and Clinic 2014;26(6):406-408,412
Objective To detect the protein expression of Cripto-1 and CD34 in basal-like breast carcinoma and study the functions of Cripto-1 in basal-like breast cancer angiogenesis,invasion and metastasis.Methods Immunohistochemistry (IHC) was used to detect the expression of Cripto-1 and CD34 in 60 cases of basal-like breast cancer,40 cases of non basal-like breast cancer and 20 cases of normal breast tissues.The clinical relationship between Cripto-1 and basal-like breast cancer were analyzed as well as its connection with CD34 markers MVD.Results The expression of Cripto-1 protein in basal-like breast cancer (42/60,70.00 %) was significantly higher than that of nonbasal-like breast cancer (19/40,47.50 %) and normal breast tissue (4/20,20.00 %) (both P < 0.05).The expression of Cripto-1 protein was positively correlated with the tumor size,clinical stage,lymph nodes metastasis and MVD value in patients of basal-like breast cancer (all P < 0.05),but there was no corelation with the age of the patients (P > 0.05).Conclusions The up-regulation of Cripto-1 expression may be associated with angiogenesis and development in basal-like breast cancer.The effect of Cripto-1 promoting angiogenesis may be an important factor in the acceleration of the local invasion and distant metastasis in basal-like breast cancer.
5.Expressions of Apaf-1 and Caspase-9 in ovarian serous carcinoma and their clinical significance
Xinlei WU ; Jinmei LI ; Bingxin ZHANG ; Jinku ZHANG
Clinical Medicine of China 2017;33(8):673-677
Objective To investigate the expressions of Apaf-1 and Caspase-9 protein in ovarian serous carcinoma(OSC) and their clinicopathological significance.Methods The expressions of Apaf-1 and Caspase-9 protein in 45 cases of OSC,60 cases of ovarian serous cystadenomas and 32 cases of ovarian borderline serous cystadenomas were detected by immunohistochemical SP method.The relationship between the expressions of these two proteins and the clinicopathological features of OSC and the correlation between Apaf-1 and Caspase-9 expressions in OSC were analyzed.Results The positive rates of Apaf-1 in OSC,ovarian serous cystadenoma and ovarian borderline serous cystadenoma were 24.4%(11/45),75.0%(45/60) and 46.9%(15/32),the difference was statistically significant (χ2=26.734,P<0.01).Apaf-1 expression was correlated with pathological grade,clinical stage and lymph nodes metastasis (χ2=6.318,7.565,5.554,P<0.05).The expression rates of Caspase-9 in OSC,ovarian serous cystadenoma and ovarian borderline serous cystadenoma were 28.9%(13/45),83.3%(50/60) and 56.3%(18/32),the difference was statistically significant (χ2=31.682,P<0.01).The expression of Caspase-9 was correlated with pathological grade,clinical stage and lymph nodes metastasis (χ2=5.750,4.391,5.466,P<0.05).In OSC,the expressions of Apaf-1 and Capase-9 were positively correlated (k=0.433,P=0.003).Conclusion The low expressions of Apaf-1 and Caspase-9 in OSC may be related to the occurrence and development of OSC.
6.Changes of serum IL-6 and IL-8 levels in patients with gastric cancer and the clinical relationships with Helicobacter pylori infection
Sanjun DU ; Qibin LYU ; Duo LI ; Huibin GAO ; Jinku SHEN
Journal of Chinese Physician 2017;19(2):250-252,256
Objective To explore the changes of serum interleukin-6 (IL-6),and interleukin-8 (IL-8) in patients with gastric cancers and the clinical relationships with Helicobacter pylori (Hp) infection.Methods Totally 62 cases of patients who were diagnosed gastric cancer in our hospital from January 2013 to September 2015 were selected as the study group,who did not accept anti-tumor therapy.The patients were divided into the Hp positive group (with 53 cases) and Hp negative group (with 9 cases) according to whether the merger of Hp infection.Another 60 normal healthy volunteers of the same age in our hospital for health examination during the same period were selected as the control group.The serum IL-6 and IL-8 levels were detected,and the changes of the factors in the study group and control group were compared and analyzed,as well as the U values of Hp infection.The clinical relationships between the expression levels of the two in patients with gastric cancer and the U values of Hp infection were confirmed.Results The serum IL-6 and IL-8 levels and the U values of Hp infection of the study group were significantly higher than those of the control group (P < 0.05),which in the Hp positive group were significantly higher than those in the Hp negative group (P < 0.05).The levels of serum IL-6 and IL-8 in patients with gastric cancer showed positive correlations with the U value of Hp infection (r =0.457,0.531,P < 0.05).Conclusions The serum IL-6 and IL-8 levels in patients with gastric cancer are much higher than those in normal healthy people,and there are significant positive relationship with Hp infection.It can improve the curative effect of gastric cancer patients by improving the eradication rate of Hp.
7.Serum matrix metalloproteinase-9 and advanced glycation end-products in diabetic patients with or without diabetic foot problems
Zhihong LI ; Hui FANG ; Yunliang ZHANG ; Shuqin GUO ; Jinku ZHANG ; Haiyan LI ; Yanfeng ZHEN ; Yue YANG
Chinese Journal of Endocrinology and Metabolism 2010;26(12):1061-1062
Serum matrix metalloproteinase-9 (MMP-9)and advanced glycation end products (AGE)levels were measured in the diabetic patients with or without foot problems as well as in healthy retired people. In diabetic patients serum MMP-9 and AGE levels were significantly higher than those in healthy subjects. Median MMP-9 level in patients with diabetic foot was higher than that in diabetic patients without foot ulcers. Increased serum MMP-9 in diabetes might predict occurance of diabetic foot and poor wound healing of the foot ulcers.
8.Effects of C-pseudonucleosides bearing thiazolidin-4-one as immunostimulants on differentiations of human lymphocytes
Ming MENG ; Chunxiao LI ; Yang HONG ; Jinku ZHANG ; Hongmei LIU ; Dongzhi CHEN ; Hua CHEN ; Xiaoliu LI
Chinese Journal of Microbiology and Immunology 2012;32(6):486-490
Objective To explore the effects of C-pseudonucleosides bearing thiazolidin-4-one as immunostimulants on differentiation and activation of human lymphocytes. Methods Peripheral blood mononuclear cells (PBMC) were isolated from healthy adults,and then incubated with immunostimulants (CH1a,CH2a,CH1b,CH2b and pidotimod).After 48 h,we collected the supernatants and then detected the concentrations of IL-2,IL-4 and IFN-γ using ELISA.After 72 h,the proliferation was detected using MTT method.PBMC incubated with immunostimulants (CH1a,CH2a,CH1b,CH2b and pidotimod),after 72 h,the cultural cells were collected and CD expressions of lymphocytes were analyzed by flow cytometry.Results All samples could stimulate proliferation of T cells.Immunostimulants CH1a,CH2a and pidotimod could elevate the expressions of CD3,CD4,CD19 and CD16CD56,and stimulate the secretions of IL-2 and IFN-γ. Immunostimulants CH1b and CH2b could elevate the expressions of CD3,CD4,CD19 and CD16CD56,and stimulate the secretions of IL-2 and IL-4. Conclusion Immunostimulants CH1a and CH2a could differentiate Th0 into Th1 and promote the proliferation of B cells as well as NK cells.However,immunostimulants CH1b and CH2b could differentiate Th0 into Th2 and promote the proliferation of B cells and NK cells.
9.The application value of HPV E6/E7 mRNA detection in paraffin-embedded samples for cervical cancer screening
Xing ZHOU ; Jinku ZHANG ; Wenming ZHAO ; Xue CHEN ; Bingjuan ZHOU ; Hong CHEN ; Jinmei LI
Cancer Research and Clinic 2014;26(4):243-245
Objective To explore the application value of detecting HPV E6/E7 mRNA in cervical cancer screening and prognosis.Methods bDNA technology was used to detect the HPV E6/E7 mRNA levels in 301 cases of paraffin-embedded cervical samples that were grouped based on pathological diagnosis.Statistic differece between multiple groups were assessed by chi-square segmentation method.Results The positive detection rate of HPV E6/E7 mRNA is higher in cervical cancer group than that in CIN1-3 groups and cervicitis group,which were 94.29 % (66/70),30.30 % (20/66),50.00 % (19/38),59.46 % (44/74),5.66 % (3/50),respectively (all P < 0.004 5).The HPV E6/E7-positive rates in all three CIN groups were higher than that in cervicitis group (P < 0.004 5).The positive rates in CIN2 and CIN3 group were higher than that in CIN1 group (P < 0.004 5).HPV E6/E7 mRNA levels in cervical cancer group were higher than those in CIN groups and cervicitis group (16 508.730±8 741.402,929:337±460.880,3 239.681±1 447.399,5 286.224±2 933.445,4.941±2.263,respectively,all P < 0.05).CIN groups had higher copy numbers of HPV E6/E7 mRNA than cervicitis group (P < 0.05).More copies of HPV E6/E7 mRNA were detected in CIN3 group than those in CIN1 group (P < 0.05).Conclusions The positive rate and expression levels of HPV E6/ E7 mRNA was related to the severity of cervical diseases.HPV E6/E7 mRNA detection in paraffin-embedded samples have a high clinical application value in predicting the risk of cervical cancer.It' s an effective measure for cervical cancer screening and follow-up.
10.Expression of HIF-1αand Glut-1 in molecular subtypes of breast carcinoma and their rela-tionship with basal-like breast carcinoma
Bingjuan ZHOU ; Jirui SUN ; Wenming ZHAO ; Xue CHEN ; Haizhi QIAO ; Bingxin ZHANG ; Jinmei LI ; Jinku ZHANG
Chinese Journal of Clinical and Experimental Pathology 2015;(9):991-995
Purpose To study the expression of HIF-1αand Glut-1 in the molecular subtypes of breast carcinoma and their correlation with basal-like breast carcinoma. Methods 803 cases of invasive breast carcinoma from our database were identified. The clinicopath-ologic findings and the biologic markers including estrogen receptor ( ER) , progesterone receptor ( PR) , and human epidermal growth factor receptor-2 (HER-2) status were reviewed. Immunohistochemical MaxVision stains for cytokeratin 5/6 (CK5/6) and epidermal growth factor receptor ( EGFR) were performed. All breast carcinomas were subclassified into Luminal A, Lumincal B, HER-2 over-expression, normal-like, and basal-like subtypes according to Nielsen criteria. Immunohistochemical stain was also used to detect the expression of HIF-1αand Glut-1. Results Positive expression rates of HIF-1αprotein in basal-like, HER-2 over-expression, normal-like, Luminal A and Luminal B substypes were 77. 89% (74/95), 56. 06% (37/66), 55. 76% (92/165), 31. 97% (141/441), 25. 00% (9/36), respectively. The positive expression rates of Glut-1 protein were 80. 00% (76/95), 57. 58% (38/66), 58. 18%(96/165), 34. 01% (150/441), 25. 00% (9/36), respectively. The positive expression rates of HIF-1α and Glut-1 in the basal-like, HER-2 over-expressing and normal-like subtypes were remarkably higher than that in Luminal A and Luminal B subtypes ( P<0. 004 5) and the expression of HIF-1a and Glut-1 was negatively correlated with the expression of ER (P<0. 01). In the ER-negative breast cancers, the positive expression rates of HIF-1a and Glut-1 in basal-like substype were much higher than that in the other sub-types (P<0. 004 5), and the expression of HIF-1α was positively correlated with expression of Glut-1 in basal-like breast carcinoma (P<0. 01). Conclusion The overexpression of HIF-1αand Glut-1 may be closely related to the ER-negative breast cancer and HIF-1α and Glut-1 might play an important role in the development of basal-like breast carcinoma.