Objective To explore the impact of TIMP 3 regulated by miR-181b as a target gene on invasion and migration of hepatocellular carcinoma (HCC) in vitro.Methods The expressions of miR-181b were detected using SYBR Green real-time fluorescence quantitative polymerase chain reaction on liver cancer specimens and on HCC cell lines.The protein expression of TIMP 3 in HCC was detected using westen blot,and SKHep-1 as a cell line expressing high miR-181b was chosen through reporter gene experiment.TIMP-3 as a target gene regulated by miR-181b and its effect on invasion and migration treated by anti-miR-181 b were studied using transwell and cell scarification test,respectively.Results The expression of miR-181b in HCC was higher than cancer-adjacent tissues and normal liver tissues.The differences among them were significant.There was a correlation between the high expression of miR-181b and invasiveness and metastasis in HCC.The protein expression of TIMP-3 in HCC was significantly lower than normal liver tissues and cancer-adjacent tissues.Expression of miR-181b mRNA was detected in various HCC cell lines such as Hep3B,HepG2,Huh 7,SKHep-1,SNU182,SNU449 and hepatocyte,with the expression of miR-181b in SKHep-1 being the highest (P＜0.01).TIMP3-3UTR was low when the expression of miR-181b was high (P＜0.05).The invasion and migration abilities of SKHep-1 were significantly inhibited by anti-miR-181b (P＜0.05).Conclusion The data suggested that miR-181b promoted invasion and migration of SKHep-1 by down-regulating TIMP-3 in HCC.

Objective:To analyze the impact of multi-color non-woven packaging in surgical instrument management on surgical turnover efficiency,surgical instrument preparation,and satisfaction of surgical medical staff and patients.Methods:31,589 surgical instruments from Beijing Friendship Hospital Capital Medical University from March 2016 to December 2021 were Selected.According to different management methods,71,007 surgical patients and corresponding 16,224 surgical instruments from March 2016 to January 2019 were included in the control group,which adopted traditional gauze-cotton bag packaging surgical instrument management method;62,548 surgical patients and the corresponding 15,365 surgical instruments from February 2019 to December 2021 were included in the observation group,which adopted multi-color non-woven packaging surgical instrument management method.The surgical turnover efficiency,surgical instrument preparation and satisfaction of surgical staff and patients were compared between the two groups.Results:The room entry time,room entry to skin incision time,the following surgery room entry to skin incision time,and surgical delay rate of surgeries of patients in the observation group were lower than those in the control group,the difference was statistically significant(t=52.681,37.167,72.524,x2=9.605,P<0.05).Compared with the control group,the number of outgoing deliveries,assembly errors and loss of instruments decreased significantly,the number of intact devices was increased significantly,the difference was statistically significant(x2=107.345,11.782,8.727,66.223,P<0.05.The satisfaction of patients and medical staff in the observation group was significantly higher than that in the control group,the difference was statistically significant(t=100.723,6.756,P<0.05).Conclusion:The application of multi-color non-woven packaging in the management of surgical instruments can help to improve surgical turnover rates,improve the efficiency of the central sterile supply department by reducing the number of outgoing deliveries,assembly errors and instrument losses,and improve the satisfaction of surgical medical staff and patients with multi-color non-woven packaging of surgical instruments.