Objective To observe the difference of sCD40L level in different subtypes of coronary heart disease,and to explore the clinical value of CD40 ligand in identification for the acute coronary syndrome(ACS).Methods The patients with coronary heart disease(CHD)and the heathy controls in the First Affiliated Hospital of Harbin Medical University from Jan,2006 to June,2006 were enrolled in the study.CHD group included ACS and stable angina patients.Enzyme-linked immunosorbent assay was used to measure the serum sCD40L level in three groups;t-test and receiver operateing characteristic curve were used to analyze the results.Results sCD40L level was significantly higher in patients with ACS[(2.39?0.37)?g/L]than stable angina patients[(1.89?0.16)?g/L,P0.05.The ROC curve demonstrated that using the concentration of sCD40L 2.01 ?g/L as the boundary to diagnose ACS,sensitivity of this marker was 0.91 and specificity was 0.80 respectively,and area under the curve(AUC)was 0.907.Conclusion sCD40L level which is more than 2.01 ?g/L has important significance for the diagnosis of ACS.

Objective To investigate the change of heart rate turbulence in patients with chronic heart failure and relationship to age、LVEF、LVED、heart rate before ventricular premature complex (VPC)、coupling interval、compensatory interval、the number and origin of VPC.Methods HRT was measured in 30 CHF patients and 30 healthy controls.HRT onset and slope were measured by the original definitions using Holter records and compared with the clinical factors.Results The HRT TS was significantly lower in patients wtih heart failure than in control (3.17?2.03vs9.64?6.47,P

dichloromethane,since the former four components account for more than 90% of the total HRV,which proposed as the target compounds in risk management of the VOCs.

Objective To evaluate the expression of Fas Ligand (FasL) on gastric T cell during H.pylori infection and its cytotoxicity to gastric epithelial cells. Methods FasL protein expressions on mono nuclear cells of gastric mucosa with and without H.pylori infection were examined by immunohistochemistry assay. FasL mRNA expressions were detected in gastric T cell lines isolated from H.pylori infected and uninfected gastric biopsies by RT PCR. The function of FasL expressed by gastric T cells to induce apoptosis in Fas bearing cells was determined by a co culture bioassay (JAM), while the Fas mediated apoptosis of gastric epithelial cells induced by gastric T cells were also evaluated by the same assay. Results FasL was expressed by the mononuclear cells accumulated in gastric mucosa with H.pylori infection, whereas the few mononuclear cells presented in uninfected gastric tissues were negative for FasL. FasL mRNA was detected in gastric T cells isolated from H.pylori infected gastric biopises, while that of uninfected gastric T cell lines was either negative or weak positive. Gastric T cells were capable of inducing apoptosis of Fas positive jurkat cells, while this could be blocked by Fas blocking antibody, ZB4. Conclusions T cells presented in gastric mucosa during H.pylori infection could damage gastric epithelium through Fas/FasL interaction.

Objective To investigate the depression in stroke patients with broca aphasia.Methods Aphasia Depression Rating Scale (ADRS) and Zung's self-rating depression scale (SDS) were applied in patients on the first stroke with broca aphasia (Aphasia group,66 cases).The results were compared with stroke patients without aphasia(control group,66 cases).Results Compared to control group,the incidence of depression in Aphasia group was significantly higher(62.12% vs 25.76%,P

ObjectiveTo investigate the neural mechanism of processing the salient target during the visual search task with spatiotemporal patterns of event-related potential(ERP).MethodsSixteen subjects were asked to judge the existence of a low or high salient target from 4 items,in which one distractor might be also salient or not.A two-way repeated-measure ANOVA was carried out for behavioral performance and ERP data( distractor saliency:YES,NO)*( target:none,low salient,high salient).ResultsSignificant interaction was found between target and distractor for the reaction time (F( 1.99,29.79) =21.56,P=0.00).For the low salient target,significant difference of reaction time was found between the YES (552.57 ± 82.32 ms) and NO (540.47 ± 75.68 ms)of distractor saliency( t (15) =- 2.489,P =0.025 ).However,such difference of reaction time was not significant for the high salient target.The statistical parametric mapping of F-value of ERP suggested the effect of the distractor saliency revealed in the right fronto-parietal-temporal areas related to stimulus novelty and the effect of the target saliency appeared in the parietal-occipital regions related to visual processing during the same stage ( 350 ～ 450ms ).The significant interaction occurred in the left temporal regions (450 ～ 600 ms ) related to stimulus familiarity and its classification.ConclusionDue to both mechanisms of the parallel processing for the target and the distractor during the perceptual stage and the effective classification of the familiar network within left hemisphere during the stage of pattern classification,the salient target can be processed quickly in spite of the interference from the salient distractor.

Objective To evaluate the clinical diagnostic value of a new nonlinear detection of index for coronary heart disease.Methods The inpatients suspected with coronary heart disease were randomly selected from March 2008 to February 2009 in the departments of The First Affiliated Hospital of Harbin Medical University,after routine examination.The time was fixed in the night before coronary angiography for data acquisition.Those whose number of cardiac RR interval (utilization) was less than 90% were removed and eventually a total of 124 cases were selected.Basing on gold standard (coronary angiography),the patients were divided into a group of 53 cases of coronary heart disease,middle group of 38 cases and the control group of 33 cases.In a stable state,the supine subjects were under the 12-lead ECG monitoring for 30 minutes to obtain RR interval data,and after careful examination data were blindly transferred to professionals who filtered the data,analyzed them with formula of 50-step chaometry(50SCM)and output.Finally,the date were analyzed with statistical methods.Results Between the three groups there was significant difference in 50SCM(P

To investigate the effects of Baoganning on injury of liver cells induced by hydrogen peroxide (H 2O 2) in rats.Rat models of injury of liver cells were induced by H 2O 2 at final concentration of 2 mmol/L. Six kinds of suspension of isolated rat liver cells were prepared according to the addition of serum. They were group A ( blank control), group B (H 2O 2 model), group C (normal control), group D (vitamin E), group E (low dosage of Baoganning) and group F (high dosage of Baoganning). Superoxide dismutase (SOD) activity, nitric oxide (NO) and malonyl dialdehyde (MDA) contents and glutamic pyruvic transaminase (ALT) level were detected.SOD activity and NO and MDA contents were increased and ALT level was decreased in Baoganning groups.[Conclusion]Baoganning exerts a protective effect on injury of liver cells induced by H 2O 2 in rats.

Objective: To investigate the protective effect of curcumin on carbon tetrachloridlde (CCl 4) intoxicated primary cultured rat hepatocytes. Methods:The rat hepatocytes were separated by perfusion with collagenase solution. Curcumin was added to the culture medium after incubating the rat hepatocyte for 36 hours. At the same time, CCl 4 was added to induce hepatocyte injury for 1 h. The levels of ALT, LDH, NO, SOD, MDA and cell viability after cultivation for 3 h were determined, respectively. Results:Curcumin significantly improved cell viability, inhibited the decrease of SOD activity and increase of the ALT, LDH, MDA, NO levels. Conclusion: Curcumin protected effectively the primary cultured rat hepatocytes against injury induced by CCl 4.

AIM:To investigate the role of Shengmai Powder (Radix Ginseng, Radix Ophiopogonis and Fructus schisandrae) (SMP) in treatmen of hemorrhagic shock in rabbits. METHODS: The concentrations of endothelin (ET), tumor necrosis factor (TNF), ? endorphin (? EP) and nitric oxide (NO), the activity of nitric oxide synthase (NOS) in plasma were respectively tested by different biochemical assays in hemorrhagic shock in rabbits. RESULTS: SMP could obviously decrease the ET,TNF,NO level and NOS activity ( P