Objective To evaluate the effects and possible mechanism of endovascular irradiation using liquid 32 P-filled ballon catheter on restenosis after interventional therapy.Methods 54 Wistar rats were randomly divided into injured group, which received the balloon injury of thoracic aorta, and irradiated group, which received the balloon injury of thoracic aorta followed immediately by ionizing radiation using 20 Gy or 28 Gy liquid 32 p-filled balloon catheter. The expressions of proliferation cellular nuclear antigen(PCNA) in vascular cells and smooth muscle actin(?-SM actin) in the vascular adventitia were detected by immunohistochemical method, and were quantified by computer image analysis. The morphologic changes of thoracic aorta were analyzed by computer image analysis . Results ⑴14 days after injury, both the lumen area and external elastic lamina (EEL) area of thoracic aorta in the irradiated group were significantly larger than those in injuried group, but the neointima area was significantly smaller in the irradiated group. The above chanages were negative relation with the irradiation doses. ⑵At third day after injury, the cell proliferation activity in the adventitia and media of thoracic aorta in the irradiated group obviously decreased in dose dependent manner compared with the injured group. At the 7th day after injury,there was not significant difference in the cell proliferation in the adventitia and media of the vessels between the irradiated group and injury group. ⑶At 7th and 14th days after injury. The ?-SM actin expression level in the adventitia of thoracic aorta in the irradiated group was significantly lower than that in the injured group, which was negatively related with the irradiation dose.Conclusion To some extent, there was a correlation between the irradiation dose of using liquid 32 p-filled balloon catheter and the areas of lumen, EEL and neointima. The endovascular irradiation could contribute to inhibiting the neointima and improving the vascular remodeling by inhibiting vascular cell proliferation and adventititial ?-SM actin expression.

Objective To discuss the influential factors of postoperative pulmonary complications in elder patients accepted abdominal operation. Methods The clinical data of 95 cases accepted abdominal operation were analyzed retrospectively,and all the patients aged over 60. These patients were divided into two groups based on whether they had pulmonary complications or not. Seven relative factors including the age,sex,smoking history,surgery duration ,operation place,pulmonary function,anesthesia were analyzed and the relationship between these factors and the incidence of postoperative pulmonary complications were explored,too. Results Among the 95 patients,19 cases had pulmonary complications. The surgery duration in patients with pulmonary complications ((3.4 ± 1.1)h)were significantly higher than in those without pulmonary complications ((2. 6 ± 0. 9)h)(P ＜0.05). We also found significant differences in the comparison of FEV1%(59.2 ±9.5),FEV1/FVC(61.3 ±7.7)% ,MVV%(59.3 ±8.6)% in the group with pulmonary complications,with FEV1%(79.7 ± 11.5)%、FEV1/FVC(73.2 ±8. 3)% 、MVV%(74. 2 ±6. 4)% in the group without pulmonary complications. The epigastric operation and general anesthesia before operation were risk factors for post-operative pulmonary comphcations(P ＜0. 05). Conclusions Surgery duration,operation place,pulmonary dysfunction and anesthesia may be the risk factors of pulmonary complications after abdominal operation.

Objective To investigate the depression in stroke patients with broca aphasia.Methods Aphasia Depression Rating Scale (ADRS) and Zung's self-rating depression scale (SDS) were applied in patients on the first stroke with broca aphasia (Aphasia group,66 cases).The results were compared with stroke patients without aphasia(control group,66 cases).Results Compared to control group,the incidence of depression in Aphasia group was significantly higher(62.12% vs 25.76%,P

Objective To investigate the related factors of postoperative pulmonary complications with abdominal operation. Methods 193 cases after abdominal operation were reviewed. The age, smoking history, surgery duration, surgical site, pulmonary function, artery blood gas analysis, and the incidence of post-operative pulmonary complications were analyzed. Results Pulmonary complications were found in 29 cases. The age was higher and surgery duration was longer in patients with pulmonary complications than those without pulmonary complications (P<0.05). The patients with abnormal forced expiratory volume in the first second (FEV1)%, FEV1/ forced vital capacity (FVC)%, maximum ventilatory volume (MVV)% and smoking history before operation were in higher risk for post-operative pulmonary complications (P<0.05). There was significant difference in artery blood gas analysis between the 2 groups (P<0.05). Conclusion The age,smoking history, surgery duration and pulmonary dysfunction may be the risk factors of pulmonary complications after abdominal operation,while it is important to monitor the blood gas analysis.

Objective To investigate the risk factors of post-operative cardiovascular or pulmonary complications in patients with gastrointestinal tumor. MethodsThe data of 352 cases with gastrointestinal tumor were analyzed retrospectively. These patients were divided into the two groups based on with or without cardiovascular or pulmonary complications. Seven relative factors including the patients' age, sex, smoking, duration of surgery, pulmonary function, ECG, UCG, and relationship between these factors and the incidence of post-operative cardiovascular or pulmonary complications were analyzed.ResultsForty-two cases occourred cardiovascular or pulmonary complications in 352 patients. The mean age and surgery duration were (61.2±7.9) years and (3.0±0.9) hours in patients with cardiovascular or pulmonary complications, and (53.5±4.6) years and (2.7±0.7) hours in patients without cardiovascular or pulmonary complications respectively. There was a significant difference between the two groups ( P<0.05). The patients with abnormal FEV1%, FEV1/FVC%, MVV% and cardiac function before operation had higher risk for post-operative cardiopulmonary complications ( P<0.05).ConclusionAge, duration of surgery and cardiac and pulmonary dysfunction may be risk factors of cardiovascular or pulmonary complications after gastrointestinal surgery.

Objective To understand the status and influencing factors of Shenzhen resident population′s inpatient hospital medical institutions selection .Methods Conducting site investigation of Shenzhen resident population ,to analyze that w hich hospi‐tal to choose ,willingness ,satisfaction and trust of resident population need to be hospitalized .Results The municipal hospital was the first choice for the Shenzhen resident population need to be hospitalized ,the degree of willingness ,satisfaction and trust of mu‐nicipal hospital were higher than the district hospital;health status and insurance coverage were the influence factors of hospitaliza‐tion medical institutions ,the worse health conditions and insured residents to choose the municipal hospital more likely .Conclusion Positive education ,guide residents reasonable medical treatment ,improve the whole service level of district hospitals ,to further improve the medical security system of Shenzhen city .

Purpose　To inquire into the clinical and pathological features of subcutaneous panniculitis-like T-cell lymphoma. Methods　The histopathology and immunophenotypes of subcutaneous panniculitis-like T-cell lymphoma in 8 cases were observed. Results　Clinically one or more subcutaneous nodules were first found, then the lesion infiltrated up to the epiderm or down to the fascia. Half of the cases were stable for long time without systematic symptoms. The others got high fever with bone marrow, lymphonode involvement, had tumescent liver, and spleen, and decreasing of the counts of thrombocytes or whole blood cells. A few were accompanied with lethal hematophage syndrome. Some rapidly advanced and died less than 3 years. Pathologically, atypical large, midium-size and small lymphocytes with variable ratio rounded the lipocytes like rosettes and were confluent to small patchs or foci or formed a reticular pattern. There were many karyokineses and karyorrhexis made of distinct karyorrhexis bodies. The histocytes reactively proliferated accompanied with phagoerythrocytic phenomena, and polynuclear giant cells and granulomatoid reaction were found. The tumor cells infiltrated in the lipolubules. They expressed T-cell immunophenotype. Conclusions　This tumor is a special type of skin lymphoma involving subcutaneous fat tissue and special clinicophathological features.

To evaluate the effect of problem based learning(PBL)in clinical education of pacemaker implanting,30 postgraduates of clinical medicine were arranged into the PBL education group and 60 into the traditional education group.The results showed that the scores of theory and clinical skill of the PBL group were significantly better than those of the traditional education group. It also showed that PBL teaching method is more helpful and more effective than traditional education in clinical education of pacemaker installing.

Objective The primary culture of synovial fibroblasts is a convenient tool to study the pathology and physiology of synovial tissues .An improved method was constructed in this study by C57BL /6 mice to study the mechanism of rheumatoid ar-thritis(RA) .Methods The synovium around the hip joints were collected .Attention should be paid to eliminate the egg-yolk like yellow oval substance in the middle of the synovium .The synovium was transferred into a 1 .5 mL Eppendorf tube containing 0 .5%type Ⅳ collagenase and cut into 1 mm3 blocks or so .The Eppendorf tube was placed in 37 ℃ Constant temperature orbital shaker incubator for 60 min .After digestion ,the tube was placed on the Vortex for a high-speed oscillation for 1 .5 minutes to guarantee the separation of cells .Results Within about 1 week ,the first passage was performed by the trypsin digestion method .On day 10 , the number of synovial macrophages reached the maximum and then decreased gradually .After the third generation (day 15 to 20) , the synovial macrophages generally disappeared .Vimentin was suitable for the immunofluorescence cytochemical staining for the synovial fibroblasts .The cell purity was indicated as > 95% .The cytometric analysis indicated that purity of Vimentin and CD90 .2-labelled cells was over 95% ;the purity of CD54-labelled cells was 80% approximately .Conclusion It is a simple and effective method for primary culture of synovial fibroblasts in mice .