Wound healing in the human cornea has distinctive characteristics in comparison to most other tissues in the body.The corneal wound healing response is a remarkably complex cascade mediated by cytokines,growth factors,and chemokines.The interactions in the epithelial,stromal,neural,lacrimal gland,immune cells are interwoven in the corneal response to injury.Corneal epithelial cells and basement membrane play important roles in this process.However,both of them are usually impaired during the procedures of excimer laser refractive surgery for myopia,hyperopia,or astigmatism,and complications such as overcorrection,undercorrection,regression,refractive instability,and haze may occur after surgery.A complete corneal wound healing process,the biologic and molecular processes that contribute to the healing response,and anomalies that lead to complications are critical for improving the efficacy and safety of excimer laser refractive surgical procedures.

Objective To investigate the corneal posterior surface topography characteristics of keratoconus at early stages applying the Pentacam anterior segment analysis system. Methods The present study included 43 eyes of 43 patients with keratoconus at the subclinical stage (group A), 40 eyes of 40 suspected keratoconus patients (group B), and 143 normal eyes of 143 controls (group C). Based on an examination of the Pentacam anterior segment analysis system of each subject, a series of data, including the posterior surface refractive power, the posterior surface elevation were collected. The differences among the three groups, the correlation among indices, and the area under the receiver operating characteristic (ROC) curves were analyzed. Results The mean values of the posterior surface maximum refractive power in 3 groups were -6.2 D, Q=0.5;-5.6 D, Q=0.3;and -5.5 D, Q=0.3, respectively. The mean values of the posterior surface maximum elevation were 23 μm, Q=14;11 μm, Q=8.5;and 7 μm, Q=6, respectively. The posterior surface maximum refractive power and maximum elevation among three groups were statistically different. The area under ROC (AUR) of posterior maximum refractive power and the AUR of posterior maximum elevation were greater than that of other indices in the diagnosis of early keratoconus. Conclusions Based on the results from the Pentacam anterior segment analysis system, the changes of the posterior surface refractive power and elevation are important characteristics of early keratoconus.

Objective To study the roles of TLR2 and TLR4 in the progress of invasive pulmonary aspergillosis(IPA) in experimental mice.Methods The mice were divided into three groups including the group of normal mice,the group of normal mice infected with A.fumigatus and the group of IPA mice.The mice were sacrificed at four time points(8 h,24 h,48 h and 72 h) after infection.The lung tissues from each group were collected for pathological analysis and RT-PcR for detecting the expression level of,TLR2,TLR4 and β-tublin.The ratio of density value of band of each PCR product on electrophoresis to the density value of β-tublin was used to evaluate the expression level of each gene like TLR2.TLR4 and TNF-α.Re-suits The pathological analysis showed the normal structure and no inflananatory reaction in the lungs in the group of normal mice.The infiltration of inflammatory cells,weak injuries and no germination of spore into hypha in the lungs of normal mice infected with A.fumigatus,and serious injuries like destruction of alveolar structure,bleeding,infiltration of inflammatory cells and germination of spore into hypha in the lungs of IPA mice.The expression level of TLR4 at 8 h,24 h,48 h and TNF-α at 24 h and 48 h were lower in IPA mice than that in healthy mice with infection(P<0.05).Conclusion There was low expression of TLR4 and TNF-α in IPA mice lung tissues.Typical pathological injuries in the lungs and germination of spore into hy-pha in IPA mice were observed by the microscope.

Objective　To evaluate the effect of modified suction ring in laser in situ keratomileusis(LASIK) for special eyeballs.Methods　Those eyeballs that couldn't be sucked successfully with regular suction ring were performed with modified suction ring, and the characteristic of those eyes was analyzed.Results　Fifteen eyes of 9 cases were included in this study and were sucked successfully with modified suction ring with intact corneal flap.Eight eyes of 5 cases had small corneal power especially in the peripheral section. The corneal power in the peripheral section of 7 eyes was less than 41D. Corneal diameter of 1 eye was 9.5mm and 6 eyes of 3 cases had small palpebral fissure.Conclusions　The modified suction ring is safe and effective for those eyes with flat cornea, small corneal diameter and small palpebral fissure.

Objective In the early life period when eyes grow rapidly,visual experience can play an important role in axial growth and refractive development. For instance, depriving the eye of form vision during infancy will accelerate axial growth, resulting in substantial amounts of myopia, called form deprivation myopia (FDM). Similarly, imposing the eye with a negative lens produces compensating myopic growth in many species, called defocus induced myopia (DIM) . As one of the important visual experiences,color vision and its effects on eye growth deserve to be investigated. The purpose of this study was to investigate the effect of 530 nm monochromatic light and establish an innovative model of myopia in guinea pig by exposing to this monochromatic light. Methods Twenty male guinea pigs at 2 weeks old were randomly assigned to two groups (n = 10) . The experimental group was raised under the condition of 330 nm monochromatic light illumination. The control one was bred under white light illumination with 5000 k color temperature. These guinea pigs were raised in a specially designed cage. The light source was provided by specially made LEDs (green: peak value 530 nm and half bandwidth 30 nm; white: color temperature 5000 K) . The illumination parameters of the two groups were identical and the light quantum number was 3 x 10~(-4)μmol·cm~(-2)·s~(-1) . Through measuring,the irradiance value was 0.150 mW·cm~(-2) for green light and 0.247 mW·cm~(-2) for white light approximately. All animals were kept under a 12/12 h light/dark cycle (light: 8 a.m. - 8 p.m. ) ,in the temperature of 22℃ - 26℃ and a relative humidity of 55% -65% . Both groups underwent biometric measurement including refraction,corneal curvature and axial length,etc. before and after twelve weeks treatment. The refraction was examined using a streak rednoscope and trial lenses in a dark room one hour after topically administering a cycloplegic eye drop. The radius of comeal curvature was measured with a keratometer (Topcon OM-4, Japan) and axial components was measured by A-scan ultrasonagraphy ( Opticon Hiscan A/B).Repeated measurements were undertaken. Only the right eye's parameters of each guinea pig were used for analysis. Unpaired (-tests were used in all comparisons between the two groups of eyes with a statistical analysis software (Stata, version 7.0) . Results Before treatment, the refraction of experimental group was 4.6 ± 0.59 D.and that of the control group was 4.63 ± 0.48 D. The axial and vitreous body length was 7.48 ±0.11 mm and 3.16 ±0.07 mm in the experimental group,respectively,and 7.55 ±0.16 mm,3.21 ±0.09 mm in the control. The differences between the biometric parameters of the two groups including refraction, comeal curvature and axial components were not significant ( P > 0.05) . However,after a twelve-week exposure, the variation of refraction in the experimental group was -3.125 ± 0.76 D,and - 1.075 ± 0.71 D was observed in the control group. There was a 2.0 D myopia in the experimental group compared with the control. Axial length grew 0.98 ± 0.13 mm in the experimental group and 0.77 ± 0.22 mm in the control. Vitreous body extended to 0.33 ± 0.14 mm and 0.13 ± 0.14 mm in the two groups, respectively. The refraction of the experimental group shifted more towards myopia ( P < 0.0001) accompanied with a more accelerated speed of axial growth and vitreous body's extension ( P < 0.05) compared with that of the control group. There were no significant differences in the radius of comeal curvature, the depth of anterior chamber and the lens thickness between the eyes of the two groups at the end of the experiment ( P > 0.05) . Conclusion The 530 run monochromatic light can accelerate the prolongation of axial length and vitreous body inducing axial myopia in guinea pigs.

Objective To compare the high risk factors, complications, treatment and prognosis of respiratory distress syndrome (NRDS) in neonates at different gestational age (GA). Methods Between August 2012 and July 2013, 156 neonates with RDS were selected and distributed into 3 groups, 42 early preterm (GA<34weeks), 52 late preterm (GA 35 to 36 weeks), and 62 in term group (GA≥37 weeks). Retrospectively analysis was performed for high risk factors, complications, treatment and prognosis of the three groups. Results In 156 neonates with RDS, the male and female proportion was 2.25:1. All groups had more males, but the gender difference has no statistical signiifcance in three groups (P=0.923). The onset time of RDS and the hospitalization time both show an increasing trend of statistical signiifcance (P<0.05). Comparing the difference of high risk factors for RDS of the 3 groups, birth asphyxia, placental abnormalities, multiple pregnancy, premature rupture of membranes was most common in early preterm group, and followed by late preterm group, and C-section was most common in term group and unexplained preterm was more common in early preterm group than that in late preterm group (all P<0.05). Among the three groups, the ratio of pulmonary surfactant application was the lowest in the term group, the ratio of X-ray grade over II was high-est in early preterm group, oxygen and hospitalization time were the longest in early preterm group (P<0.05). The risks of com-plicated with pulmonary infection, intracranial hemorrhages and bronchopulmonary dysplasia were the highest in early preterm group and the risk of complicated pneumothorax was the highest in term group. Among three groups, the recovery rate was the lowest in the early preterm group (P<0.01). Conclusion The clinical characteristics, high risk factors, complications and treat-ment responses of RDS in neonates with different GA were different, so GA should be considered for diagnose and treatment. For the term infants, the elective caesarean section should be strictly controlled, in order to reduce the incidence of RDS.

Objective:To observe the lens zonule infrastructure changes in experimental myopia. Methods; The eye lids were sutured to induce the myopia in guinea pigs and the zonule ultrastructure changes were observed. Results: There were significant differences in the eye axial length and diopter between lid-sutured eyes and controls(P

0.05),but the numbers of CD41+ cells and platelets were increased significantly(P

Objective To analyze the association of miR-326 mRNA expression level on regulatory T cells between clinical manifestations of patients with systemic lupus erythematosus (SLE), in order to inves-tigate the role of miR-326 on Treg cells and pathogenesis as well as its association with disease activity of SLE. Methods Twenty milimeter anti-coagulated peripheral blood was obtained from 52 SLE patients and 20 healthy controls. Treg were purified by MACS from peripheral blood, in which the quantity of miR-326 and Ets-1 mRNA were assessed by real-time polymerase chain reaction (PCR). Data were analyzed by using Mann-Whitney U test and Spearman correlation analysis. Results Compared with healthy controls [0.921(0.345, 1.879)], the expression of miR-326 mRNA level was significantly higher in Treg from SLE patients [2.927 (0.518, 8.662) (Z=-3.756, P<0.05). The difference between new-onset SLE patients [8.878(5.922, 12.466)] and recurrence group [3.512(0.582, 11.223)] with healthy controls was significant (Z=-2.135, Z=-4.928, P<0.05). The expression level of miR-326 in new-onset SLE patients was higher than inactive SLE patients (Z=-4.928, P<0.05). Significant difference of the expression level of miR-326 mRNA was found between new-onset SLE patients with serous cavity effusion [7.606(0.656, 16.795)] and new-onset SLE patients without it [1.840(0.576, 13.025)](Z=4.263, P<0.05). Our analysis showed that significant positive correlation was found between the expression of miR-326 mRNA in Treg with CRP (rs=0.481, P<0.05) and anti-C1q antibody (rs=0.729, P<0.05) from new-onset SLE patients. Conclusion The expression level of miR-326 is upregulated in Treg from SLE patients and is associated with disease active index, which suggests that miR-326 in Treg may participate the pathological process and disease activity in SLE.