A method was developed for the determination of 39 polybrominated diphenyl ethers(PBDEs) from mono- to hepta-brominated in coastal sediment by gas chromatography/mass spectrometry. The sample was extracted with a solution of dichloromethane: n-hexane( 1:1,V/V) ,followed by ultrasonic extraction for 60 min at 25℃. The clean-up step was optimized using silica gel and alumina. The detection limit of the method was in the range of 0.003 - 0.10μg/kg. The results indicate that the average recovery of the internal standard was from 66.2% to 118. 6%. The relative standard deviation RSD( % ) was from 0. 8 % to 18. 2%. The method for the detection of 39 PBDE congeners in coastal sediment was sensitive,high reproducible with satisfactory recoveries.

Objective To explore the influence of contrast agent injection rate and sequence time resolution on the dynamic contrast-enhanced MR iamging of brain glioma.Methods Fifty eight cases with suspected cerebral glioma were scanned on a Verio 3.0T MRI with the regular head sequences and DCE sequences.All the cases were divided into two groups.There were 41 cases in the regular group in which the injection rate was 3 mL/s and the time resolution was above 5.36 s.Another 1 7 cases were in the optimal group in which the injection rate was 4 mL/s and the time resolution was about 4 s.The DCE images of two groups were Postprocessed by the Tofts model analysis.The curve shape of AIF were evaluated and divided in to the excellent or the secondary level.Chi-square test was used to compare the results of two groups.Results The curve shape of optimal group was much better than that of regular group(P<0.05).Conclusion Speeding up the injection rate and the time resolution of DCE sequences would improve the image quality of DCE MRI of glioma.

【Objective】 To explore the intention of voluntary blood donation in students from a middle vocational school in Jinhua and its influencing factors. 【Methods】 A total of 400 students were enrolled from a middle vocational school in Jinhua by convenience sampling, and a self-designed questionnaire was issued to each student to investigate the cognition, intention and attitude of voluntary blood donation. The influencing factors of voluntary blood donation intention were analyzed by Logistic regression. 【Results】 A total of 394 valid questionnaires(98.50%) were collected. For 394 surveyed students, the average score of cognition of voluntary blood donation was (7.27±2.69) points, and the overall rate of intention to voluntary blood donation was 21.32%. Logistic regression analysis equation consisted of family support, better cognition, feeling happy about blood donation and worrying about blood donation, with risk ratios(OR) as 31.78, 188.69, 26.27 and 0.01, respectively(all P<0.05). 【Conclusion】 Students from a middle vocational school showed poor congnition and intention of voluntary blood donation.Family support for voluntary blood donation, better cognition of voluntary blood donation and happiness for blood donation were positive factors, and the fear of blood donation was a negative factor.It is necessary to strengthen the publicity of blood donation among students in middle vocational school to improve their congnition and intention to voluntary blood donation.

Objective To investigate the effects of ramipril on nitric oxide(NO) concentration and endothelial nitric oxide synthase(eNOS) activation in lung of rats with pulmonary arterial hypertension induced by monocrotaline(MCT).Methods Sixty Sprague-Dawley rats were randomly divided into three groups: normal control group,MCT group and ramipril group.Rats in MCT group and ramipril group were subcutaneously injected with 60mg/kg of MCT.Then the rats in ramipril group received ramipril gavage and rats in MCT group received normal saline gavage for 4 weeks,respectively.Rats in control group were subcutaneously injected with normal saline first and then received normal saline gavage for 4 weeks.Right ventricular systolic pressure(RVSP) and right ventricle hypertrophy index(RVHI) were measured.The ratio of arteriol wall thickness/vascular external diameter(WT%) and wall area percentage(WA%) were evaluated.NO concentration in lung was determined.eNOS,P-Ser1177-eNOS and Akt phosphorylation were analyzed by Western blotting.Results The RVSP,RVHI,WT%,WA% were significantly increased,and NO concentration,the level of eNOS,P-Ser1177-eNOS,the state of Akt phosphorylation were significantly decreased in MCT group compared with that in control group(P

The purpose of this study was to reseach the relationship between the upper gastroin- testinal endoscopy and heart rare and blood oxygen saturation (SaO2 ) , 62 patients were examined by (esophagogastroduodenoscopy , EGD ) and monitored with a transcutaneous pulse oximeter simultane- ously. The mean age was 44. 5 years (range 21 - 68) , 30 males and 32 females. No sedative drugs and at- ropin were used. The mean pulse and SaO2 value before endoscopy were 94. 12/min and 97. 68%. During the ECD SaO2 was slightly decreased by a mean of 1 . 61% ( P 100/min accounted for 61 . 3%, it was more than preendoscopy (P

ObjectiveTo study the efficacy of immunosuppressant IL-1RA on the rat model of penetrating keratoplasty.MethodsCorneal transplantation were performed orthotopically from Lou rats to F344 rat's recipients.Animals were randomly assigned to the following groups:1.Control.2.CsA(3mg/kg/2d,subconjunctival injection).3.IL-1RA(0.5mg/kg/2d,subconjunctival injection).Treatment began at the 1st day postoperatively and continued until the 14th day postoperatively.Mean survival times and rejection index were determined.ResultsThe mean survival time of the control group was 12.235±1.674 days,but the mean survival time for CsA and IL-1RA were 17.676±1.528 days and 19.250±1.456 days,respectively,which was statistically significant compared with the control group(P<0.01).Vascularization index in IL-RA group was markedly lower when compared with the control and CsA groups(P<0.01).ConclusionIL-1RA can markedly prolong the survival time of corneal allografts by inhibiting graft rejection,and prevent vascular growth of corneal allografts

Objective To investigate the relationship of uteroglobin gene polymorphism to the sus-ceptibility to, clinical type and pathological type of Henoch-Schsnlein purpura (HSP) and Henoch-Schonlein purpura nephritis (HSPN). Methods Totally, 118 patients with clinically diagnosed HSP, including 80 cases of HSPN and 38 cases without renal involvement were recruited in this study together with 100 normal human healthy controls. Genomic DNA was isolated from peripheral blood leucocytes of all subjects. The uteroglobin G38A polymorphism was determined by PCR-restriction fragment length polymorphism (RFLP). Results The frequencies of genotypes 38GG, 38GA and 38AA in normal human controls did not differ from those in patients with HSP, patients with HSP but without nephritis, patients with HSPN, patients with HSP and joint involvement, patients with HSP and gastrointestinal involvement (all P > 0.05). Also, no sig-nificant difference was observed between patients with HSPN and patients with HSP but without nephritis (P > 0.05). Furthermore, the frequency of genotypes 38GG, 38GA and 38AA had no significant correlation to the clinical phenotype of HSP, the occurrence of gross hematuria and nephrotie syndrome or the degree of renal damage (all P > 0.05). A significant increase was observed in the frequency of genotype 38AA in patients with HSP with elevated serum IgE compared with those with normal serum lgE (58.82% vs 8.43%, χ2 = 21.946, P < 0.05, OR = 15.51, 95% CI range: 4.93% - 48.84%), whereas the frequency of genotype 38GG was significantly increased in patients with HSPN and hypertension than in those with HSPN but without hypertension (75.68% vs 18.60%, χ2 = 26.172, P < 0.05, OR = 13.61, 95% CI range: 5.01% -37.01%). Conclusions The uteroglobin G38A polymorphism seems unrelated to the susceptibility to and degree of renal damage in patients with HSP and HSPN. The genotype 38AA may be associated with elevated level of serum IgE In patients with HSP, while genotype 38GG is associated with a high incidence of hyper-tension in patients with HSPN.

Objective To study the value of 3D-CT reconstruction in the diagnosis of surface involved lesions of bone for its nature and location,comparing with plain film and 2D-CT.Methods Surface-involved lesions of bone revealed by plain-film were performed spiral CT examination.Multi-planar reconstruction(MPR)and surface shading display(SSD)were performed among 20 cases which had pathologic results.The diagnosing correct rate of nature and location in all the techniques were compared.Results The correct rate of nature diagnosing of plain film,2D-CT,MPR and SSD was 75%,80%,65%,60% respectively;correct rate of location diagnosing was 90%,75%,85%,95% respectively.The highest nature diagnostic correct rate was found in 2D-CT.The highest location diagnostic correct rate was found in SSD.Conclusion Spiral CT stereoscopic reconstruction is of great value in the location diagnosing of surface-involved bone lesion.

Objective To discuss the efficiency and safety of transjugular intrahepatic portosystemic shunt(TIPSS) in treatment of cirrhotic patients with gastroesophageal variceal bleeding.Methods52 patients who had gastroesophageal variceal bleeding were treated with TIPSS,with 11 patients of them being active bleeding,23 patients being moderate to massive ascites.Results50 patients were treated successfully with the successful rate of 96.2%.The pressure of potal dropped from 3.87?0.50kPa to 2.45?0.40kPa(P

Objective To examine the change of TGF-?1 in the bloods of rats which were induced by tetrachoride during the different times and to explain the mechanism of the change.MethodsThe experimental liver fibrosis induced by tetrachloride in rats was treated by matrine compound injection.Serum TGF-?1 and pathological changes in liver tissue were observed in control group,model group and therapy group during the time of the 4th,6th and 9th week.ResultsThere was an obvious change of TGF-?1 in the blood in different time and different groups.ConclusionMatrine compound injection has an antifibrogenetic action and TGF-?1 can reflect the change of liver fibrogenesis of different time as an index.