Objective To summarize our experience in microsurgical treatment of dumbbell tumors. Methods The clinical features,surgical approaches,operative techniques and surgical results of 21 cases of dumbbell tumors were analyzed retrospectively.Four surgical approaches were adopted according to the size and location of tumors,including,posterior midline approach(in 7 cases),modified far lateral approach(in 5 cases),posterior midline supplied by anterior cervical approach(in 2 cases)and paramidline approach(in 7 cases). Results In this series,10 cases of tumors were situated in the cervical,6 in thoracic,4 in lumbar and 1 in sacral segment of the spine.Total removal were achieved in 95% (20/21),and subtotal removal in 5%(1/21).Postoperative pathological diagnosis revealed 15 neurinomas,5 spinal meningiomas and 1 metastatic adenocarcinoma.14 cases of meningiomas and 1 metastatic adenocarcinoma were followed up for 1 to 48 months.11 of the 14 patients recovered completely,with 3 of them living daily life independently,4 maintainly radical hypoesthesia,and one having decubital ulcer unhealed.No postoperative malformation and dysfunction of the disposed spine occurred in these patients.Magnetic resonance imaging scans in 11 of the 14 follow-up cases revealed no tumor recurrence,of which inhanced MRI scans were performed in 8 cases. Conclusions dumbbell tumors should be treated by one-stage operation.Microsurgical technique could improve the rate of total removal,and decrease postoperative complications.

Objective Effect of preventing recurrent pterygium excision combining amnion transplant by using recombinate interferon aIb drip eyes. Methods 105 cases of pterygium excision and combining amnion transplant patients were divided two groups,one group was treated by interferon aIb dripping eyes for 50 pterygium, another group was treated by 0.1%fu mi long drip eyes liquid to drop eyes as a contrast group. Results 50 patients in treatment group were followed up a case by regular visits to months after the operation and none of them had not been seen pterygium recurrent after passing 12～24 months and average time was 15 months;55 patients in contrast group had 6 recurrents,recurring rate was 10.9%,the statistic difference was significant( P

Current situation and existed problems on bilingual teaching of molecular biology in medicine in our university were described in brief in present paper.According to actual teaching conditions of our university,and the developmental tendency on bilingual teaching in our state,some suggestions on promoting bilingual teaching of molecular biology in medicine,such as,bilingual teaching materials selection,course arrangement,teaching methods reformation,teachers training etc,were provided.

Objective: To observe the effects of linezolid on the levels of inflammatory factors and T lymphocytes in patients with severe tuberculosis. Methods: Sixty patients with severe tuberculosis treated in Jinhua Guangfu Hospital from April 2016 to November 2017 were selected and divided into control group(n=30) and observation group(n=30) according to different treatment options.The control group was treated with personalized anti-tuberculosis regimen.The observation group was treated with linezolid on the basis of the control group, and both two groups were treated for 9 months.The changes of interleukin-1(IL-1), tumor necrosis factor alpha(TNF-α), IL-6, IL-10 and CD₃⁺, CD₄⁺, CD₈⁺ and CD₄⁺/CD₈⁺ were observed before and after treatment.The incidence of liver injury, thrombocytopenia, myelosuppression, nausea and vomiting, diarrhea were observed. Results: Before treatment, there were no statistically significant differences in IL-1, TNF-α, IL-6, IL-10, CD₃⁺, CD₄⁺, CD₈⁺, CD₄⁺/CD₈⁺ between the two groups(all P>0.05). At 9 months after treatment, the levels of IL-1, TNF-α, IL-6 and IL-10 in the control group were (10.94±1.31)ng/L, (3.03±0.49)ng/L, (183.43±13.24)ng/L, (134.93±34.51)ng/L, respectively, which in the observation group were (6.89±1.29)ng/L, (2.49±0.45)ng/L, (129.48±10.74)ng/L, (189.35±43.27)ng/L, respectively, the differences between the two groups were statistically significant(t=12.195, 11.214, 8.414, 11.291, all P<0.05). The CD₃⁺, CD₄⁺, CD₈⁺, CD₄⁺/CD₈⁺ in the control group were (47.61±7.16)%, (15.49±6.64)%, (20.58±5.61)%, (0.79±0.19) , respectively, which in the observation group were (65.46±8.31)%, (30.23±7.85)%, (34.59±7.41)%, (0.87±0.24) , respectively, the differences between the two groups were statistically significant(t=10.497, 7.865, 12.128, 10.291, all P<0.05). There were no statistically significant differences in the incidence of liver function impairment, thrombocytopenia, myelosuppression, nausea and vomiting, and diarrhea between the two groups(all P>0.05). Conclusion Linezolid in the treatment of severe tuberculosis can reduce the level of inflammatory factors, improve the cellular immune level of patients, and has a lower incidence of adverse effects.

Objective Based on the result of evaluating the nutritional state of in-patients using the methodologies of European Nutritional Risk Screening 2002(NRS 2002),we judged the rationality of nutritional therapy,and the rate of accepting nutritional therapy in hospital patients.Methods Totally 3567 inpatients consisted with the requirement of NRS2002 were investigated nutritional state and the situation of nutritional therapy within 2 weeks in hospital.Except BMI used as the standard of China,all the other methods were from NRS2002.Nutritional therapy included parenteral and enteral nutrition.Results In all objects,834 patients' number score was≥3(23.39%),and 256 got nutritional therapy among them(30.70%).The number of accepting nutritional therapy was 661(18.53%),and only 38.73% of them had a patient number score≥3.Conclusion NRS2002 is a good method to evaluate the nutritional state of in patients.The nutritional therapy should be on the basis of patient's nutritional state,but there isn't one specification in clinical nutrition-support.Many malnourished patients were ignored,while a lot of nutritional therapies were not suitable.Now one of method to solve this question is to set up Nutrition Support Team(NST).

The paper discusses the experimental teaching reform of biochemistry by referring to the characteristics of biochemical development and special feature.The reform increases effectually the interests of medical students,and may contribute to their creative competence and the abilities of scientific research.

Objective To investigate the treatment of neonatal respiratory distress syndrome by less invasive surfactant administration(LISA)clinical efficacy,complications and influence on blood gas analysis.Methods A total of 100 cases of neonatal respiratory distress syndrome requiring pulmonary surfactant(PS)tracheal injection therapy and admitted to the Jinhua Central Hospital between December 1st 2019 and August 1st 2022 were randomly assigned into treatment group(50 cases)and control group(50 cases).The treatment group of children received PS treatment using LISA technology,while the control group of children received PS treatment using tracheal intubation tracheal instillation PS extubation followed by intubation-surfactant-extubation technology.The clinical treatment effect,blood gas analysis,non-invasive ventilation time,length of hospitalization,and incidence of complications were compared between the two groups.Results The total effective rate of treatment in the treatment group was higher than that in the control group;After 1 hour of PS treatment,the oxygen partial pressure and blood oxygen saturation of the treatment group were higher than those of the control group,while the carbon dioxide partial pressure was lower than that of the control group.The non-invasive assisted ventilation time of the treatment group was shorter than that of the control group.The incidence of bronchopulmonary dysplasia was lower than that of the control group.Their difference were statistically significant(P<0.05).Conclusion The use of LISA technology for PS treatment of NRDS can improve blood gas indicators,shorten non-invasive ventilation time,improve diagnostic and therapeutic effects,reduce the risk of bronchopulmonary dysplasia,and do not increase the incidence of complications such as tracheal mucosal injury and pulmonary hemorrhage in children.

Objective To study the correlation among property, efficacy and target of herbal Chinese materia medica; To analyze the molecular mechanism of cold and heat property of Chinese materia medica; To provide references for explaination of microcosmic mechanism and scientific connotation of property of Chinese materia medica. Methods Recordings about property and efficacy of single medicine in 2015 edition of Pharmacopoeia of the People's Republic of China were sorted out. According to Pharmacological Research of New Ideas and New Targets and Pharmacology, 509 kinds of herbal Chinese materia medica were selected. Relevant articles about property, efficacy, pharmacologic action, and target in CNKI and Chinese Academic Journal Database (Wanfang Data) were searched by computers. The target information was screened and standardized, and the database was constructed by using MySQL5.7.13. The correlation between the property, efficacy and the target of the herbal Chinese materia medica was studied by using the frequency analysis and correlation rule algorithm of R software platform 3.3.1. Results 509 kinds of herbal Chinese materia medica were selected, including 227 kinds of cold-property medicine, 106 kinds of neutural-property medicine and 176 kinds of hot-property medicine. According to the result of data mining, efficacy of cold-roperty medicine was quenching thirst, clearing liver and treating stranguriaetc. The target was transforming growth factor β2 and liver microsome, etc. The efficacy of hot-property medicine was warming the middle, releasing cold and dissolving lumps. The target was bone morphology protein 2, rheumatoid factor, etc. The efficacy of neutural-property medicine was clearing lungs, diminishing distension and increasing energy. The target was β-amyloid and prostaglandin E2 receptors. Conclusion There is certain correlation in property, efficacy and target in Chinese materia medica.

Objective To investigate the clinical value of glypican-3 (GPC3) in the diaganosis of hepatocellular carcinoma (HCC),the contents of GPC3 in the serum and tissues of HCC patients were detected.Methods ELISA and immunohistochemical staining were applied to detect GPC3 expressing level in the serum and tissues in 79 cases with HCC,35 cases with post-hepatitis cirrhosis and 30 normal liver specimens and the resuits were compared.The influential factor of GPC3 content in the patients with HCC was analyzed by logistic regression model.Results The serum level of GPC3 in patients with HCC was (143.02±40.26) μg/L which was signifcantly higher than that in patients with post-hepatitis cirrhosis [(6.15±4.31) μg/L] and healthy controls [(4.47±3.22) μg/L] (all P ＜ 0.01).The expression levels of GPC3 was signifeantly higher in post-hepatitis cirrhosis tumor-adjacent tissue and tumor-distant tissue.The expression levels of GPC3 was significantly positively correlated with tumor presence of distant mestasis (x2 =13.182,P ＜ 0.0) and clinical stage (x2 =4.250,P ＜ 0.05),and not correlated with sex,age,tumor size and the level of AFP inserum (P ＜ 0.01).Conclusion GPC3 is specific the diagnosis of HCC.The joint diagnosis of GPC3 and AFP will improve the sensitivity of HCC.Therefore,GPC3 could as a biomarker for evaluating HCC condition and prognsis.

OBJECTIVE:To investigate the clinical efficacy and safety of Cinobufacin capsules combined with radiotherapy in the treatment of median and advanced nasopharyngeal carcinoma. METHODS:Ninety-five patients with median and advanced naso-pharyngeal carcinoma in cancer center of our hospital during 2011-2012 were analyzed retrospectively,and then divided into con-trol group (57 cases) and observation group (38 cases) according to therapy plan. Control group received radiotherapy alone, while observation group was additionally given Cinobufacin capsule 0.5 g,tid,till the end of radiotherapy. A treatment course last-ed for 4 weeks. The prolonged radiotherapy were compared between 2 groups. Clinical response rate was observed in 2 group,and 1-year and 3-year survival rate and the occurrence of ADR were followed up. RESULTS:The proportion of prolonged radiotherapy was 89.47% in control group,which was significantly higher than 52.63% of observation group,with statistical significance(P<0.05). At the end of therapy and one month after therapy,clinical total response rates of observation group were 81.58% and 63.16%,which were significantly higher than 70.18% and 45.61% of control group,with statistical significance (P<0.05). The incidence of white blood cell lowering,hemoglobin lowering,local side effect of radiotherapy, nasopharyngeal dysphagia/odyno-phagia in observation group were significantly lower than in control group,with statistical significance (P<0.05). There was no statistical significance in survival rate between 2 groups in 1st-year and 3rd-year follow-up (P>0.05). CONCLUSIONS:Cinobu-facin capsule combined with radiotherapy can significantly relieve the side effects of radiotherapy in median and advanced nasopha-ryngeal carcinoma patients.