Objective To investigate the relationship between the expressions of Twist and clinical pathological parameters in gastric carcinoma,to explore its significance in judging the prognosis of patients,and to analyze the expression of Twist proteins in the epithelial-mesenchymal transition (EMT) phenomenon.Methods Immunohistochemistry was used to detect the expressions of Twist and EMT relative proteins E-cad,N-cad in 120 cases of gastric carcinoma specimens and corresponding adjacent normal tissue.Analysis The relationship between Twist expression and clinical pathological parameters,and the Twist expression and E-cad,N-cad were analyzed.Results In gastric carcinoma,Twist,E-cad,N-cad positive rates were 59.1％(71/120),36.6 ％ (44/120) and 47.5 ％ (57/120),and in adjacent tissues,the positive rate were 17.5 ％ (21/120),93.3 ％ (112/120) and 11.6 ％ (14/120).There were significant differences in each index between tumor tissues and adjacent tissues (P ＜ 0.05).Patients with Twist positive expression showed higher incidence rates of lymph node metastasis (P ＜ 0.05) and distant metastasis (P ＜ 0.05) than those with Twist negative expression.The expression of Twist was significantly correlated in gastric carcinoma accompanied by low expression of E-cad and abnormal high N-cad expression (P ＜ 0.05).Conclusions Compared with the adjacent tissue,the expression of Twist,E-cad and N-cad have significant differences,and the expression of Twist was closely related to the biological characteristics in gastric carcinoma.The expression of Twist in gastric cancer have negative correlation with the EMT relative factor E-cad,while positive correlation with the N-cad.

Objective:To study the differential expression of VEGF, SDF-1 and CXCR4 in colorectal cancer tissue and paracancerous tissues, and analyze the correlation between the three and the clinic pathological parameters of gastric cancer;to investigate the significance of the expression of VEGF,SDF-1 and CXCR4 in the progression of colorectal cancer.Methods:Immuno-histochemistry streptavidin-biotin complex( SABC) method and real-time Q-PCR were used to detect the operation resection of colorectal carcinoma and tumor-adjacent specimens of SDF-1,CXCR4 and VEGF expression.Results: On the mRNA level,compared with the adjacent tissues,the expression of SDF-1, CXCR4 and VEGF in colorectal cancer tissues was significant higher .The IHC′s results indicate that the positive rate of SDF-1,CXCR4 and VEGF in colorectal cancer were 78.7%,60% and 66.3%,the positive rate of adjacent tissues was 37.5%,42.5% and 28.8%, and there was statistically significant between expression and carcinoma adjacent tissue differences( P<0.05).There was statistically significant difference in the expression of SDF-1 with tumor metastasis,lymph node metastasis,TNM staging(P<0.05-P<0.01),while had no difference with gender,size and depth of invasion.There was statistically sig-nificant difference in the expression of CXCR4 with node metastasis,TNM in tumor staging ( P<0.05-P<0.01).It showed a significant negative correlation in SDF-1 expression and CXCR4,VEGF expression(P<0.01).Conclusion: It has a certain relationship between biological characteristics and SDF-1, CXCR4 and VEGF expression in colorectal cancer tissues.These results indicate that the expression of SDF-1,CXCR4 and VEGF play an important role in the tumorigenesis,progression,invasion and metastasis of colorectal cancer.

Objective To explore the distribution of inflam m atory cells and positive expression of P-se-lectin glycoprotein ligand-1 (PSG L-1) in infant brainstem tissue from hand-foot-m outh disease related fatal brainstem encephalitis. Methods Tw enty brainstem sam ples from infants suffered from brainstem en-cephalitis w ere collected as the experim ental group. Ten brainstem sam ples from infants died of non-brain diseases and injuries w ere collected as the control group. The distribution of inflam m atory cells and the expression of PSG L-1 in the tw o groups w ere exam ined by im m unohistochem ical m ethod. The characteristics of the positive cells w ere observed. Results In brainstem tissue of the experim ental group, there w ere sleeve infiltrations of inflam m atory cells around the vessels and in the glial nodule. Microglia was the m ost and following was neutrophils around the vessels and in the glial nodule. There was a significant statistical difference am ong m icroglias, neutrophils and lym phocytes (P<0.05). There was no sleeve infiltration in the control group. PSG L-1 protein was expressed w idely in inflam m atory cells in the experim ental group, especially in the inflam m atory cells around the vessels and in the glial nodule. B ut PSG L-1 positive staining could be observed significantly less in the control group com paring with the experim ental group (P<0.05). Conclusion Microglia is the m ain type of inflam m atory cells involved in the progress of the fatal disease. Moreover, PSG L-1 could participate in the pathogenesis of hand-foot-m outh disease related fatal brainstem encephalitis.

To explore the clinical features and common complications of fast resting heart rate (RHR) in hypertensionpatients. Methods: We retrospectively analyzed the entire rest electrocardiogram data of Qingdao study 2000 and Xinyang study2005 in community population elder than 18 years including hypertension patients and non-hypertension subjects. Clinical complications as diabetes, coronary artery disease, hyperlipidemia and stroke, laboratory findings, RHR in ECG, body mass index (BMI), waist to hip ratio and office blood pressure were collected in all participants. Results: A total of 18183 participants were enrolled including 61.6% male, the average age was (45.2±12.7) years including 6763 hypertension patients. Compared with normal BP subjects, hypertension patients had the faster RHR (73.5±11.6) times/min vs (70.6±9.6) times/min, P<0.001 and more hypertension patients combining fast RHR (14.5% vs 6.4%), P<0.001. In hypertension patients, compared with normal RHR patients, fast RHR patients had the elder age (53.9±12.2) years vs (51.8±11.2) years, lower BMI (25.8±3.6) kg/m2 vs (26.4±3.4) kg/m2 and higher ratio of grade 3 hypertension (68.2%vs 59.0%), all P<0.001; higher levels of fasting blood glucose (6.0±2.4) mmol/L vs (5.6±1.5) mmol/L and triglyceride (2.0±1.8) mmol/L vs (1.7±1.3) mmol/L, both P<0.001, higher LDL-C (3.2±0.9) mmol/L vs (3.1±0.8) mmol/L, P=0.001;more patients with diabetes (6.6% vs 3.9%), P=0.007 and stroke (11.1% vs 8.3%), P=0.005. Multivariate regression analysis indicated that with adjusted traditional risk factors, fast RHR was positively related to stroke occurrence in hypertension patients (OR=1.306, 95% CI 1.021-1.671). Conclusion: Fast RHR happened more in hypertension patients than in normal BP subjects; it had the increased risk for stroke occurrence in hypertension patients.

Objective To find whether CX3CR1 V249I and T280M are associated with CAD. Methods Database in English and Chinese, including PUBMED, EMbase, were searched to get the case-control studies on the associa-tion between V249I, T280M and CAD. Results Six studies were reviewed. The pooled OR of V249I compared to wild type allele was OR =0. 85(95% CI =0. 67 ～ 1. 08,P >0. 05). The pooled OR of T280M compared to wild type allele was OR =0. 82(95% CI =0. 70 ～ 0. 96, P < 0. 05). Conclusion The T280M is a protective factor of CAD, while the V249I is not associated with CAD.

Objective: To reveal intuitively the micro and macro structure of school health research in China, in order to lay a solid foundation for further study. Methods: Statistical analyses the current status of 864 high-level journal papers in the field of school health in China by EXCEL and SATI software, author cooperation and organization cooperation, research hotspots and frontier trend knowledge maps were drawn by Citespace. Results: The research in the field of school health in China shows a wavy growth trend, the contribution of Chinese Journal of School Health is the biggest(757). Ma Jun, Tao Fangbiao, Wang Zhiyong, Liu Wei, Duan Jiali, Xu Yong are high-yield authors, Peking University, Anhui Medical University, Dalian City and Beijing Center for Disease Control and Prevention are high-yield institutions. Author cooperation and institutional cooperation are relatively loose, and Duan Jiali et al. and Peking University institute of child and adolescent health, respectively, form the largest cooperation community. Status, supervision, rural area, management, surveillance, health education, epidemiology, nutrition, environmental health, infectious diseases, common diseases, and influencing factors, etc. are hot topics in the field. Conclusion Cross-boundary exchanges and cooperation should be strengthened to promote regional coordinated development. Health risk behaviors of children and adolescents, environmental health surveillance and epidemiology of infectious diseases of primary and secondary schools, the school health personnel present situation investigation, children nutrition health and common diseases prevention and control, rural school health supervision and health education countermeasures, and school health management model were the hot topics, prevention of child injury, Internet addiction, and physical and mental health promotion were the leading directions. The commnunication and cooperation between different fields should be strengthened to improve the reginal development.

OBJECTIVETo observe the effects of shoulder lift reduction for treatment of posterior dislocation of hip joint.

METHODSFrom July 2001 to June 2010, 14 cases of posterior dislocation of hip joint were treated with shoulder lift reduction involving 12 males and 2 females with an average age of 34.6 years ranging from 18 to 57 years. After recduction, all patients were assisted with the traction, exercise and traditional Chinese medicine. The mean duration between injured and treatment was 1.1 days (2 hours to 3 days). According to Harris scoring system the hip joint function were evaluated.

RESULTSAll patients were followed-up for 8 to 24 months (means 16 months). Fourteen cases were reset well in first without any complication. The Harris score was (97.14 +/- 4.90) in total, involving hip pain (42.86 +/- 1.88), articular activity (4.71 +/- 0.47), daily activity (45.57 +/- 9.26), deformity (4.00 +/- 0.00), the clinical outcome was excellent in 12 cases,good in 2. There were not complications such as avascular necrosis of the femoral head, and so on.

CONCLUSIONThe shoulder lift reduction can be used by one person, and the treatment of posterior dislocation of hip joint is effective. It established a good foundation for the rehabilitation of trouble hip.

Adolescent ; Female ; Hip Injuries ; therapy ; Humans ; Joint Dislocations ; therapy ; Lifting ; Male ; Musculoskeletal Manipulations ; methods ; Shoulder ; Treatment Outcome ; Young Adult

Humans ; Alzheimer Disease ; Hearing Loss

We isolated and examined a new Alternaria sp., which causes leaf spots on Peucedanum japonicum in Korea, by using molecular and morphological methods. Phylogenetic analysis based on a combined internal transcribed spacer region analysis and two protein-coding genes (gpd and Alt a1) demonstrated that the causal fungus was most closely related to A. cinerariae and A. sonchi, and relevant to A. brassicae. However, conidial morphology indicated that it is a novel species within the genus Alternaria, and therefore we have assigned the fungus a new name in this study.
Alternaria* ; Brassica ; Classification ; Fungi ; Korea

Objective To understand the ability of the CDC to detect ammonia nitrogen in drinking water in Hubei Province. Methods The blind samples of two concentration levels were tested in the laboratories of the disease control institutions in the province, and the test results were evaluated by double-sample Z score. Results The satisfaction rate was 76.29% (74/97), the problem rate was 15.46% (15/97), and the dissatisfaction rate was 8.25% (8/97). Conclusion The detection ability of ammonia nitrogen in drinking water in laboratories of disease control institutions in Hubei Province is generally high，however，some laboratories need to be further improved.