1.Chemical studies on root bark of Lycium chinense
Chinese Traditional and Herbal Drugs 1994;0(07):-
Object To study the chemical constituents of the root bark of Lycium chinense Mill Methods Various chromatographic techniques were used to separate and purify the constituents Their structures were elucidated on the physico chemical properties and spectral data Results Five compounds were isolated from the root bark of L chinense and identified as vanillic acid (Ⅰ), apigenin (Ⅱ), linarin (Ⅲ), glucosyringic acid (Ⅳ) and digupigan A (Ⅴ) Conclusion Digupigan A is a new compound. Except vanillic acid, others were isolated from this plant for the first time
2.Expression and significance of Ki-67 antigen in bladder cancer
Wei CHENG ; Jingyu WANG ; Dongwen WANG
Cancer Research and Clinic 2009;21(11):748-749
Objective To investigate the expression of Ki-67 in bladder transitional cell carcinoma and its relationship with grade and stage. Methods Immunohistochemistry method were used to detect the expression of Ki-67 in bladder transitional cell carcinoma and control groups. Results The positive expression rate of Ki-67 antigen in 60 cases of bladder cancer, 20 cases of benign bladder disease and 10 cases normal bladder mucosa were 25.9 %, 10.3 % and 1.1 %. There were significantly difference among each group. The more grade and stage, the greater expression of Ki-67 antigen. Conclusion Ki-67 antigen is related to cell proliferation. The expression of the Ki-67 antigen in bladder cancer is closely related to the grade extent of tumor, and it is an important cell proliferation indicator. As a signal, Ki-67 antigen reflects cell proliferation, measurement of the expression of the Ki-67 antigen could reflect the condition of tumor cell proliferation. It might be an important prognostic factor for judging the occurrence, development and prognosis of the tumor.
3.Expression of GRKs in synovial tissue from rats with collagen-induced arthritis and the effect of total glucosides of paeony
Jingyu CHEN ; Huaxun WU ; Yin CHEN ; Lingling ZHANG ; Wei WEI
Chinese Pharmacological Bulletin 1986;0(06):-
Aim To investigate the expression of GRKs in rats of synovial tissue with collagen-induced arthritis(CIA) and the effect of total glucosides of paeony.Methods SD rats were divided into six groups including normal,model,TGP(25,50,100 mg?kg-1)groups,GTW(40 mg?kg-1)group.Chicken type Ⅱ collagen was used to induce CIA in rats.The expression of GRKs was detected by Westernblot.Results The expression of GRK2,5,6 increased in model group than that in normal group.Compared with the model group,the expression of GRK2,5,6 decreased in TGP groups.Conclusion In rats of synovial tissue with CIA,the expression of GRKs was abnormal,and TGP could change the variation of GRKs which may be one of the mechanisms of TGP improvement CIA.
4.Research advance of tumor necrosis factor receptor-associated factors in inflammatory immune regulation
Ying LI ; Jingyu CHEN ; Lingling ZHANG ; Wei WEI
Chinese Pharmacological Bulletin 2015;(9):1206-1210,1211
The tumor necrosis factor receptor (TNF-R)-associated factor (TRAF )is an important of multifunctional intracellular signal transduction factors.TRAFs involve in signal transduction of many receptor families,including TNF receptor family (TN-FR),Toll-like receptors interleukin-1 receptors (TLR-IL-1R) family and so on.TRAFs play important roles in innate immunity and acquired immunity.TRAFs could directly or indirectly re-cruit the intracellular domains of receptors in the condition of ac-tivated receptor,which leads to the activation of transcription factors,such as NF-κB and interferon-regulatory factor (IRF), through signaling pathway.And TRAFs ultimately induce im-mune and inflammatory responses and involve in the development of inflammatory autoimmune diseases.
5.THE NUTRITIONAL EVALUATION OF AN ELEMENTAL DIET FROM HYDROLYSATE OF SWINE BLOOD
Qishou XU ; Hanmin CHEN ; Jingyu WEI ; Siying SUO ; Yuzhang GE ;
Acta Nutrimenta Sinica 1956;0(02):-
An elemental diet, commercially named Elementalio, contains an amino acid mixture and many other essential nutrients. The amino acid mixture is prepared from hydrolysis of swine blood and supplemented with certain crystalline L-amino acid. The animal experiments suggest that it has a better nutritional effect than other domestic elemental diets in terms of body weight gain, nitrogen balance and correction of hypoproteinemia.
7.Assessment for early embryo quality improves the sensitivity and specificity to predicting embryonic development potential
Nan LI ; Jingyu LI ; Yongmei TANG ; Jihong WEI
Chinese Journal of Tissue Engineering Research 2014;(42):6849-6855
BACKGROUND:Embryo viability assessment is directly related to the selection of embryo transplantation and clinical outcome of assisted reproduction.
OBJECTIVE:To summarize the assessment methods for early embryonic development.
METHODS:The first author searched PubMed database for relevant articles published from January 1990 to
December 2013 using the keywords of“assisted reproductive technology, art;pre-implantation embryo;embryonic development viability;evaluation methods”in English. Final y, 63 articles were included in result analysis.
RESULTS AND CONCLUSION:In the embryo quality evaluation, the most widely used method is morphological evaluation method which is characterized as rapid, non-invasive, and simple. With the development of assisted reproductive technology, the morphological evaluation combined with time-lapse imaging analysis system has been recognized in embryo selection. Recently, targeted-metabolic analysis has been proposed as a useful tool for assessment of embryo development potential, involving pyruvate acid, glycometabolism, amino acid, and embryo-derived cytokines (soluble human leukocyte antigen G1, platelet-activating factor, etc.). Furthermore, the pre-implantation genetic screening method targeting gene and chromosome abnormality is expected to find more effective markers for evaluating embryo developmental potential.
8.Conversion treatment with sirolimus in lung transplant recipients
Dong WEI ; Fei GAO ; Bo WU ; Min ZHOU ; Jingyu CHEN
Chinese Journal of Organ Transplantation 2015;36(8):490-494
Objective To explore the efficacy and safety of conversion from calcineurin inhibitor (CNI) to sirolimus (SRL) as major immunosuppressive therapy in lung transplant recipients.Method Retrospective analyses were conducted for the clinical data of all the patients undergoing lung transplantation in Wuxi People's Hospital between January 2011 and December 2014.Sixteen were given conversion treatment with Sirolimus in the postoperative irnmunosuppressive therapy.We analyzed the opportunity and reasons in the conversion treatment,and the safety,effectiveness and complications of the conversion treatment.Result The follow-up period was 8 to 25 months,and the median time of conversion was 6 months after operation (2-18 months).The indications of conversion concluded:malignant tumor (n =8),renal dysfunction (n =5),lymphangioleiomyomatosis (n =1) and intractable diarrhea caused by CNIs (n =2).Four cases suffered from interstitial pneumonitis associated with Sirolimus and one case suffered from spontaneous pneumothorax,and they all conversed back to CNIs.In those patients,cancer recurrence occurred in 4 cases (of them,there were 3 deaths),and 3 patients developed chronic rejection.Those recipients receiving the conversion treatment due to renal dysfunction showed recovery of renal function to some extent.Conclusion It's effective and safe to converse the immunosuppressive therapy based on Sirolimus.Sirolimus should be reduced or withdrawn when interstitial pneumonitis associated with Sirolimus occurred.
9.Expression of cadherin genes in chronic myeloid leukemia and its significance
Hongqin GAO ; Jingyu WEI ; Hua YAN ; Jianbin YANG
Journal of Leukemia & Lymphoma 2015;24(11):672-675
Objective To explore the expression of cadherin (Cad) genes in patients with chronic myeloid leukemia (CML),and to elucidate the significance of Cad genes in the development of CML.Methods E-Cad and N-Cad gene expression levels in bone marrow mononuclear cells (BM-MNC) from 48 CML patients (29 in chronic phase,19 in progressive phase) were detected by real time quantitative polymerase chain reaction (qRT-PCR).Results Gene expression of E-Cad and N-Cad was detectable in CML BM-MNC.E-Cad gene expression level was lower in progressive CML than that in chronic CML (0.20 ± 0.35 vs 1.19 ± 0.87,P < 0.01),while N-Cad was highly expressed in progressive CML than in chronic CML (0.89 ± 0.45 vs 0.57 ± 0.47,P < 0.05).E-Cad gene expression level was negatively corrclatcd with thc pcrccntagc of peripheral blood progenitor cells (r =-0.705,P < 0.01).Conclusion E-Cad and N-Cad gene expression correlates with the progression of CML,and might be used as an evaluation index for disease development.
10.Early respiratory infections in lung transplantation recipients from donation after cardiac death donors
Bo WU ; Ji ZHANG ; Dong WEI ; Jingyu CHEN
Chinese Journal of Organ Transplantation 2015;36(5):261-264
Objective To explore the epidemiology,etiology and prevention strategy of early respiratory infections (≤1 month) in lung transplantation recipients with donation after cardiac death donors.Method The clinical data of donors and recipients,particularly on early respiratory infections,were retrospectively analyzed in 17 lung transplantations.Result From Jan.2015 to Apr.2015,12 episodes of early respiratory infections (≤ 1 month) in 17 lung transplantation recipients occurred (12/17,70.6%).The organisms most frequently involved were bacteria:Pseudomonas aeruginosa (4/26,15.4%),Klebsiella pneumoniae (4/26,15.4%),Staphylococcus aureus (3/26,11.5%),and Acinetobacter baumannii (3/26,11.5%).Of 26 bacterial strains,3 were Methecillin resistant Staphlococcus aureus,3 were carbapenem resistant Acinetobacter baumanni,2 were carbapenem resistant Pseudomonas cepacia,2 were extended spectrum b-lactamase-producing Klebsiella pneumoniae,and one was carbapenem and quinolone resistant Pseudomonas aeruginosa.Conclusion The morbidity of early infections is high in lung transplantation recipients.In our experience,bacterial respiratory infections are most common in the early post-transplant period (≤ 1 month).Incidence of Aspergillus spp.and Cytomegalovirus pneumonia is lower than before lung transplantation,probably due to the spread of universal prophylaxis.