Objective To explore the changes of glutathione and nicotinamide adenine dinucleotide phosphate levels and their significances among elderly patients with acute myocardial infarction,elderly patients with angina pectoris and elderly people with normal coronary artery.Methods Totally 64 elderly patients with acute myocardial infarction were selected according to the clinical manifestation,electrocardiogram and myocardial markers.68 elderly patients with angina pectoris and 66 subjects with normal coronary artery (control group) were enrolled according to the coronary artery radiography.Peripheral venous blood in all subjects were taken and plasma glutathione (reduced form GSH and oxidized form GSSG) and nicotinamide adenine dinucleotide phosphate (reduced form NADPH and oxidized form NADP+) were measured.The GSH/GSSG ratio and NADPH/NADP+ redox potentials were calculated according to Nernst equation.Results Compared with control group,GSH level in acute myocardial infarction group and angina pectoris group significantly increased(4.04 ± 0.77 μmol/L,5.89 ± 0.85 μmol/L,7.55 ± 0.93 μmol/L; P＜0.05 or 0.01),GSSG decreased(0.70±0.05 μmol/L,0.61±0.04 μmol/L,0.53±0.03 μmol/L;P＜0.05 or 0.01),GSH/GSSG ratio increased(5.18±1.06,9.76±1.67,12.80±1.93; P＜0.05 or 0.01).The GSH/GSSG redox potentials decreased(-123.49 ± 1.18 mV,-126.21 ± 1.01mV,-128.71 ±1.29 mV;P＜0.05 or 0.01).Compared with control group,NADPH in acute myocardial infarction group increased(5.72 ± 0.44 nmol/L,6.83± 0.55 nmol/L ; P＜0.05),NADPH/NADP ratio increased (2.29±0.10,2.58±0.26,P＜0.05),the NADPH/NADP+ redox potentials significantly decreased (-306.3±2.44 mV,-312.1±2.53 mV; P＜0.05).Conclusions The imbalance of plasma redox status migrating to oxidization may have close relationships with atheromatous plaque formation,plaque rupture and thrombosis.

Objective To investigate the epidemiological characteristics of hand -foot -mouth disease (HFMD in Weihai of Shandong province,during 2015,and provide reference for HFMD control and prevention. Methods We analyzed data of HFMD cases in Weihai during 2015 by descriptive epidemiology.Results A total of 8 526 HFMD cases were reported in Weihai during this period,including 64 severe cases but no deaths.The reported morbidity was 304.57 /lakh.The vast majority of patients were 0 -5 years old,representing 89.16% of the patients. Most patients were in preschools or scattered home care environments,accounting for 59.60% and 35.74%, respectively.There were clearly more boys than girls,with a male to female ratio of 1.39:1,which was statistically significant(χ2 =15.013,P <0.001).Compared with the incidence of the national level,all districts in Weihai are with high incidence of HFMD,especially in Huancui district(584.02 /lakh).The cases mainly occurred during June, July.The clinical presentation was characterized by fever and various kinds of vesicles or papules at the hand,foot, mouth or buttocks.Conclusion HFMD is one of the serious health hazards communicable disease to the children under 5 years old in Weihai.According to HFMD epidemiology characteristic,immunization and treatment strategies of HFMD should be further improved.

PURPOSETo evaluate the effects and toxicites of combination chemotherapy of taxol, cisplatin and 5-flu-ouracil for the patients with advanced cancer. METHODS 7 cases /19 courses were administered by systematic chemotherapy, Taxol was given 150 mg-180 mg by intravenous (i. v.) infusion on day 1. cisplatin 40 mg i. v. on days 2 - 4. 5-fluouracil 750mg i. v. on days 2 - 4. 5 cases /13 courses were administered by intraperitoneal chemotherapy . Taxol was given 150-180 mg by intraperitoneal(i. p.) infusion on day 1. cisplatin 60 mg-100mg i. p. on day 1, 5-fluouracil 1000 mg i. p. on day 1. Course was repeated every four weeks. RESULTS Two patients showed complete response (16. 67%), eight patients showed partial response (66. 66%). the overall response rate was 83. 33%. The major toxic effects were myelosuppression, alopecia, arthralgra and myalgia. CONCLUSIONS Taxol, cisplatin. 5-fluouracil regimen is an effective treatment for patients with advanced cancer with acceptable side-effects.

Objective To investigate the dosage, efficacy and safety of intravitreal injection of plasmin in producing posterior vitreous detachment (PVD), and the possible role of plasmin in degrading adhesion glycoproteins of inner limiting membrane (ILM). Methods Twenty eyes of young human cadavers within 24 hours after death were divided into 4 groups that received 0 1 ml balanced salt solution (group 1) as control, 1 (group 2), 2 (group 3), or 3 (group 4) U of human plasmin. Optical and transmission electron microcopies were performed to examine the ultrastructure of the vitreoretinal interface. Electron immunocytochemical techniques were carried out on ILM to estimate the content of fibronectin (FN) and laminin (LN). Flow cytometry was used for cell viability analysis of variance (ANOVA) and Tukey test was employed for statistical analysis. Results Microscopy demonstrated that plasmin especially in group 4 cleaved the attachment of the vitreous collagen fibrils to the ILM with no evident damage to the inner retina. The content of LN, FN in ILM decreased with injection of plasmin (group 3 and 4 had statistical significance from control group for FN, P

Objective To discuss the clinical application of rotational DSA and its advantages. Methods 35 cases undergoing cerebral angiography and hepatic arteriography were performed with standard DSA, of which, 24 ones were performed with rotational DSA. Results Standard DSA could only display partial lesion at lateral side, while rotational DSA could display the pathological structure and surrounding structure. Conclusion Rotational DSA is valuable for the diagnosis and treatment of encephalic angioma and liver tumor.

Objective:To investigate influences of bicortical anchora ge on the values of natural frequency of dental implants. Methods: Using the commercial code of SolidWorks, 3-D models of a screw-shaped dental i mplant and a mandibular bone segment were generated. After the 3-D implant-bon e complex was meshed by ABAQUS software, effects of bicortical anchorage on the buccolingual and axial first-order natural frequencies of the implant were comp uted. Results:Bicortical anchorage increased the buccolingual na tural frequencies and the axial natural frequencies of the implants by a maximu m of 13.77% and 107.40% respectively. The apical anchorage depth in the cortical bone had an insignificant effect on further increase of implant stability. Conclusion:Bicortical anchorage can increase the stability of dental implants, especially in the axial direction.

Objective To evaluate the method and result of thyroid artery embolization as a new therapy for hyperthyroidism. Methods Twenty two patients with hyperthyroidism underwent selective thyroid artery embolization. Totally 52 thyroid arteries were embolized with microspheres. The indications to this therapy were following: hyperthyroid patients having indications to surgical and 131 I therapy, clinically being difficult to complete the preparation for subtotal thyroidectomy and having high risk for surgical process because of their huge thyroid gland. Results Serum level of thyroid hormones dropped significantly [median T3 from 8 8 nmol/L(4 3～ 43 0 nmol/L) to 4 0 nmol/L(1 1～9 2 nmol/L), median T4 from 206 4 nmol/L(77 4～748 2 nmol/L) to 144 5 nmol/L(25 8～279 9 nmol/L), P

OBJECTIVE:To prepare Koujunmie gargle,establish a quality control method and to observe the therapeutic effect.METHODS:Konjunmie gargle was prepared with method of dissolution;the contents of aciclovir,tinidazole and ciprofloxacin in the gargle were determined with HPLC and the stability test was performed.RESULTS:The method was simple,rapid and accurate.The assay of three ingredients in Koujunmie gargle could be simultaneously performed.The gargle was stable.The total effective rate was99.0%after10-day treatment(P

In order to enhance the students’knowledge of clinical medicine and lay the foundation for using clinical medicine,we adjusted the content and compiled pharmacognosy teaching materials,aiming at its professional features of clinical pharmacy of Xuzhou Medical College.The pharmacognosy teaching material has been applied in several rounds.Feedback from students suggested that using this teaching material was well-targeted,clear and practical.

BACKGROUND:Embryo viability assessment is directly related to the selection of embryo transplantation and clinical outcome of assisted reproduction.
OBJECTIVE:To summarize the assessment methods for early embryonic development.
METHODS:The first author searched PubMed database for relevant articles published from January 1990 to
December 2013 using the keywords of“assisted reproductive technology, art;pre-implantation embryo;embryonic development viability;evaluation methods”in English. Final y, 63 articles were included in result analysis.
RESULTS AND CONCLUSION:In the embryo quality evaluation, the most widely used method is morphological evaluation method which is characterized as rapid, non-invasive, and simple. With the development of assisted reproductive technology, the morphological evaluation combined with time-lapse imaging analysis system has been recognized in embryo selection. Recently, targeted-metabolic analysis has been proposed as a useful tool for assessment of embryo development potential, involving pyruvate acid, glycometabolism, amino acid, and embryo-derived cytokines (soluble human leukocyte antigen G1, platelet-activating factor, etc.). Furthermore, the pre-implantation genetic screening method targeting gene and chromosome abnormality is expected to find more effective markers for evaluating embryo developmental potential.