Objective To evaluate the dynamic change of the antithrombase and fibrinolytic function in the critical patients.Methods Eighty-seven patients hospitalized in ICU were studied. According to the clinical manifestation, all patients were divided into systemic inflammatory response syndrome (SIRS) group ( n = 68) and non-SIRS group ( n = 19), or multiple organ dysfunction syndrome (MODS) group ( n = 37) and non-MODS group (n = 50) respectively. Thirty-one healthy volunteers were selected for control in the same period. The intravenous blood samples were taken 1,3 and 5 days after ICU admission to measure platelet,count (PLT) , and the plasma levels of antithrombase activity (AT: A), plasminogen activity (PLG: A), fibrinogen (FIB) and D-dimer (D-D) concentrations.Results AT: A, PLG: A and PLT were lower and FIB and D-D levels higher in all patients than control levels (P

AIM: To observe the changes of inflammatory factors and blood coagulatory factors and effects of antithrombin Ⅲ (ATⅢ) on activated inflammatory factors and blood coagulatory factors in rats with hemorrhagic shock. METHODS: The rat model of hemorrhagic shock was set up. 40 SD rats were randomized into four groups: sham operation, shock, routine dose ATⅢ and high dose ATⅢ groups, each group was composed of 10 SD rats. Shock group was administered common resuscitation fluid, routine dose ATⅢ group was administered ATⅢ 20 U/kg, high dose ATⅢ group was administered ATⅢ 100 U/kg everyday for successive three days. Plasma NF-?B, 6-Keto-prostaglandinF1?, E-selectin, sICAM-1, thrombin-ATⅢ complexes, thrombinogen fragment F1+2 (PF F1+2), D-dimer and TMD levels were detected. RESULTS: Plasma NF-?B, sICAM-1, E -selectin levels were significantly lower in high dose ATⅢ group than those in shock group and routine dose ATⅢ group (P

Objective:To explore the protective effects and mechanisms of salidroside on cerebral ischemia-reperfusion injury in middle cerebral artery occlusive(MCAO)rats.Methods:Focal cerebral ischemia in rats was produced by 1.5 h occlusion of the middle cerebral artery and 24 h reperfusion.Salidroside was administered intravenously immediately after occlusion and reperfusion respectively.The neurologic deficit scores were investigated according to Zea-Longa's Standard.The infarct areas were assessed with software Imagepro Plus 6.0 after TTC staining.And the activities of inducible nitric oxide synthase(iNOS)as well as glutathione peroxidase(GSH-Px)were determined by specific kit.The levels of B cell lymphoma/lewkmia-2(Bcl-2)and Bcl-2 assaciated X protein(Bax)in central neutral system were assessed by western blot.Results:Compared with the model group,salidroside could decrease the neurologic deficit score,reduced the infarct areas of the brain in MCAO-I/R rats,increased the activity of GSH-Px and decreased the activity of iNOS,enhanced the expression of Bcl-2,depressed the expression of Bax.Conclusion:Salidroside has a protective effect on cerebral ischemia-reperfusion injury and this effect is related to attenuating lipid peroxidation,enhancing the expression of Bcl-2 and diminishing Bax expression.

Objective To study the clinical characteristics、risk factors and preventive measures in the patients of hospital-acquired septicemia in ICU.Methods Retrospective survey was carried out in 76 patients with hospital-acquired septicemia from 2002 to 2005.Results The hospital-acquired septicemia was related to the underlying disease、aggressive procedure and long duration of combined antibiotics.The most bacteria were Gram-negative bacilli.Fungemia must be given reconstruction.Conclusions It is important to reduce the aggressive procedures and reasonably use antibiotics in the prevention of the hospital-acquired septicemia for patients.

Objective To investigate the clinical effect of treatment of severe carbon monoxide poisoning with Naoxingjing injection combined with naloxone.Methods Emergency department of affiliated theorem clinical college, hospital 70 patients diagnosed with severe carbon monoxide poisoning from emergency department in June 2014 to December 2016, were randomly divided into observation group and control group 35 cases.In the control group were treated with naloxone treatment, observation group were treated based on the use Naoxingjing injection treatment.The two groups were observed and recorded recovery time, mortality, the incidence of delayed encephalopathy, Glasgow Coma Score(GCS)and serum levels of IL-1β.Results The patients in the observation group total effective rate of 97.14% was significantly higher than 77.14%, and the difference was statistically significant(P<0.05);the recovery time was observed in patients(2.25 ± 0.88)h, significantly shorter than the control group(3.46 ± 1.10)h, and the difference was statistically significant(P<0.05);and follow-up observation group mortality rates were delayed encephalopathy(1.43%, 8.57%)than the control group(17.14%, 25.71%), and the difference was statistically significant(P<0.05);④ observation group were GCS score was (12.77±1.89), significantly better than the control group, and the difference was statistically significant(P<0.05);⑤ observed in patients IL-1β is(65.41±9.93)ng / L was significantly lower than the control group(89.86±10.74)ng / L, and the difference was statistically significant(P<0.05).Conclusion Naoxingjing injection combined with naloxone treatment of severe carbon monoxide poisoning exact clinical effect, can effectively reduce patient recovery time, reduce mortality and morbidity.

Objective To investigate the expression of Ki-67,Survivin,Livin in dysplasia,colon carcinogenesis and para-carcinoma tissues,and to discuss the variation of cell proliferous capability in colon carcinogenesis and antiapoptosis factors.Methods 219 specimens were composed of mild,moderate and severe atypical hyperplasia,well,moderately,poorly differentiated adenocarcinoma,para-carcinoma tissues of 36,34,18,35,27,35 and 34 cases.Detected the expression of Ki-67,Survivin and Livin with tissue microarray and immunohistochemical methods.All data were statistically analyzed by SPSS 17.0.Results In mild,moderate and severe atypical hyperplasia,well,moderately,poorly differentiated adenocarcinoma and para-carcinoma tissues the expression of Ki-67 were (21.56 ± 19.20)％,(37.44 ± 17.41)％,(36.17 ± 17.41)％,(55.29 ± 16.13)％,(44.89 ± 29.67)％,(45.11 ± 29.24)％,(43.94 ± 28.84)％,Survivin were 13.8 ％,44.1％,77.8 ％,85.7 ％,85.1％,91.4 ％,91.1％,and Livin were 2.7 ％,38.2 ％,55.6 ％,100.0 ％,77.8 ％,80.8 ％,79.4 ％.The differences of Ki-67,Survivin and Livin expression in each group were statistically significant (F =6.796,X2 =81.754,X2 =95.200,all P ＜ 0.05).Ki-67 was significantly correlated with expression of Livin (r =0.360,P ＜ 0.05) and no correlated with expression of Survivin (r =0.044,P ＞ 0.05).Conclusion Colonic epithelium from mild atypical hyperplasia to proliferation of well-differentiated adenocarcinoma cells formed a peak,and gradually down to poorly differentiated adenocarcinoma formed a platform.When the colon epithelial cells to become cancerous,the capability of cell proliferation will significantly enhance,apoptosis inhibition will reach the peak and the tumor cell will happen the changes of malignant biological behavior.Tumor microenvironment may promote the cell proliferation in para-carcinoma tissues and the development of colon cancer.Livin may inhibit apoptosis and promot the progression in synergistic mechanism importing with Survivin,which play a role in the development of colonic adenocarcinoma.

Objective To study nursing countermeasures for the brain tumor surgery patients with intracranial hypotension syndrome (IHS).Methods A total of 68 IHS patients in our hospital after brain tumor operation were divided into study group (n =34) and control group (n =34) with random number table method.Control group took routine nursing intervention,and comprehensive nursing intervention was given to study group on the basis of control group.Intracranial pressure recovery time,length of hospital stay,and the changes of quality of life after 6 months of two groups were compared.Results The intracranial pressure recovery time and hospital stay time were lower than that in control group (P ＜ 0.05).Psychological function,life condition,social function and physical function score after 6 months were higher than that in control group (P ＜ 0.05).Conclusion Comprehensive nursing intervention can effectively shorten intracranial pressure recovery time for IHS patients and hospital stay time,and improve the quality of life,so it is suitable for clinical application.

Objective To study nursing countermeasures for the brain tumor surgery patients with intracranial hypotension syndrome (IHS).Methods A total of 68 IHS patients in our hospital after brain tumor operation were divided into study group (n =34) and control group (n =34) with random number table method.Control group took routine nursing intervention,and comprehensive nursing intervention was given to study group on the basis of control group.Intracranial pressure recovery time,length of hospital stay,and the changes of quality of life after 6 months of two groups were compared.Results The intracranial pressure recovery time and hospital stay time were lower than that in control group (P ＜ 0.05).Psychological function,life condition,social function and physical function score after 6 months were higher than that in control group (P ＜ 0.05).Conclusion Comprehensive nursing intervention can effectively shorten intracranial pressure recovery time for IHS patients and hospital stay time,and improve the quality of life,so it is suitable for clinical application.

Rhodiolae Crenulatae Radix et Rhizoma is a precious traditional Chinese medicine and has extensive pharmacological effects, such as anti-hypoxic, anti-fatigue, anti-aging, anti-inflammatory, anti-tumor, lowing blood sugar, heart and brain protection. It has been applied to the treatment of cardiovascular diseases, nervous diseases, respiratory diseases, endocrine diseases, fracture problems, emotional stress, and skin diseases. The present review summarized the literature about its clinical use, which would provide a reference for further utilization.

Objective To explore effect of Yiqi-Huoxue decoction in combination with exercise rehabilitation therapy on functional recovery of hemiplegic limbs and surface electromyography (Surface electromyography,sEMG) during cerebral infarction convalescence.Methods According to random number table,80 cerebral infarction patients in convalescence stage who met the inclusion criteria were divided into the combination therapy group and rehabilitation therapy group,40 in each group.Rehabilitation therapy group received hemiplegia rehabilitation therapy;combination therapy group took Yiqi-Huoxue decoction on the basis of rehabilitation treatment.The 2 groups were treated for 3 months.In hemiplegic upper limb,Fugl-Meyer Assessment (FMA) was scored and Root Mean Square (RMS) and Median Frequency (MDF) of sEMG on biceps brachii were tested before and after each course of treatment respectively.Results After 1-,2-,or 3-month treatment,FMA score of combination therapy group was significantly higher than that of rehabilitation therapy group (t=2.702,4.595,4.444,respectively;all P＜0.01).At 2 or 3 months after treatment,the RMS of combination therapy group (t=7.188,5.405,respectively;all P＜0.01) and the MDF (t=3.336,4.584,respectively;all P＜0.01) were significantly higher than that of rehabilitation therapy group.Conclusions The Yiqi-Huoxue decoction in combination with exercise rehabilitation therapy can improve motor function and muscle tension of hemiplegic limbs during cerebral infarction convalescence.