An intravenous immunoglobulin preparation(IVIG)in solution was pasteurized,or heated at 60℃ for 10 hours in the presence of 50g/dl xylitol as stabilizer.The results show- ed that the antibody levels against diphtheria,measles viruses and HBs Ag,of the pasteurized IVIG,were not changed,the Fc function of IgG molecule was not damaged,the anti- complement activity was reduced significantly,and its stability was improved.Both model viruses,the cytomegalovirus and the simian retrovirus D1,added in the prepara- tion,were inactivated rapidly under the above-mentioned conditions as those of IVIG pasteurization.It was suggested that the pasteurized IVIG preparation not only fully saved its biological activity,but also would be better tolerance for intravenous injection and the safety in preven- tion of viral disease transmission.

The ?_2-macroglobulin(?_2M)pre- paration was pasteurized,using 40g/dl of xylitol as a stabilizer,to reduce the risk of transmission of the viral diseases such as AIDS and hepatitis during the clinical trans- fusion.The ?_2-M preparation,treated by heating at 60℃ for 10 hours, can still preserve fully its trypsin- binding biological activity and im- munological reactivity,and no new antigens were detected,which had the behavior in eleetrophoresis and the catabolic rate in rabbit that were not different from those of the un- heated samples,indicating that the pasteurized ?_2-M preparation still keeps its good native properties.

The increase in the maximum time of collected whole blood storage at room temperature from 8 to 24 hours should bring about the versatile benefits. For making the mentioned above change it was necessary not only to demonstrate the blood components prepared from the whole blood stored at room temperature for 24 hours should still keep their functions, but also to indicate that the increase in the time of whole blood storage at room temperature couldn't accelerate the bacterial proliferation. Therefore, the two units of whole blood were pooled,inoculated with bacteria, split again into two units,stored at room temperature for 8 and 24 hours, respectively,and then the blood components were separated. The levels of the bacteria found in the blood components were measured in the process of their storage, indicating that the whole blood stored at room temperature for 24 hours couldn't accelerate the proliferation rate of 5 common contaminating bacteria existed in the blood components in their storage process.

Objective To research the effect of Hydroxyl radical(type Ⅰ mechanism)and singlet oxygen(type Ⅱ mechanism)on virus inactivation and red cell cytotoxicity induced by DMMB and M007 phototreatment.Methods Hydroxyl radical quenchers mannitol and glycerol,singlet oxygen quenchers sodium azide and tryptophan were employed to investigated their ability to inhibit the inactivation of bacteriophage Phi6 and red cell cytotoxicity by induced DMMB and M007 phototreatment.Results Singlet oxygen quenchers could inhibit the inactivation of bacteriophage Phi6 obviously, whereas hydroxyl quenchers hardly affect the inactivation of bacteriophage Phi6.Both type of quenchers could reduce the hemolysis caused by CMMB or M007 phototreatment.Conclusion Viral inactivation of DMMB and M007 phototreatment is mainly through type Ⅱ mechanism, both type I and type Ⅱ mechanisms contribute to red cell cytotoxicity.

Objective To observe the efficacy of bloodletting therapy plus narrow band ultraviolet B (NB-UVB) in treating prurigo nodularis.Method According to the randomized controlled principle, the enrolled patients were divided into a treatment group and a control group. The treatment group was intervened by bloodletting cupping at the selected acupoints and the topical areas plus NB-UVB once every other day; the control group was by orally taking Mizolastine sustained release tablets and external application of Halometasone cream.Result The total effective rate was 85.7% in the treatment group versus 61.9% in the control group, and the difference was statistically significant (P<0.01).Conclusion Bloodletting therapy plus NB-UVB can produce a content efficacy in treating prurigo nodularis, with few adverse reactions.

9.9Log10 bacteriophage R 17 Conclusion Alkylation of the phenothiazine ring increased the potential of inactivating virus and decreased the damage to red blood cells? M007 might be an alternative phenothiazine dye that can be used to inactivate viruses in red blood cells?

Virus inactivation with photochemistry is being suitable for blood or blood products, methylene blue (MB)/light treatment has been used for viral inactivation of cellular blood components. Twelve new phenothiazines derivatives were designed and synthesized, and were used to test viral inactivation and red cell damage preliminary. Results showed that compound YWW-7 has a satisfactory activity, it could be developed as a new viral inactivation agent for blood products.

Objective To measure serum levels of 25-hydroxyvitamin D (25HVD) in patients with chronic urticaria (CU),and to explore its role in the occurrence of CU.Methods Peripheral blood samples were obtained from 50 patients with CU and 40 healthy controls.The urticaria activity score (UAS) was used to assess the severity of CU and to group the patients with CU.Enzyme-linked immunosorbent assay (ELISA) was performed to measure the serum levels of 25HVD,interferon-γ (IFN-γ),interleukin-4 (IL-4) and immunoglobulin E (IgE).Statistical analysis was carried out by t test,rank sum test and linear correlation analysis.Results The serum level of 25HVD was significantly lower in the patients with CU than in the healthy controls (15.20 ± 7.72 vs.21.54 ± 8.31 μg/L,t =3.75,P ＜ 0.05).Moreover,there was a significant difference in the distribution of serum 25HVD levels between the patients and healthy controls (H =17.9,P ＜ 0.05).However,no significant difference was observed in the serum level of 25HVD between severe and mild CU groups (15.57 ± 7.38 vs.14.86 ± 6.28 μg/L,t =0.37,P ＞ 0.05).Compared with the control group,the patient group showed significantly decreased serum levels of IFN-γ (t =15.34,P ＜ 0.05),but increased serum levels of IL-4 and IgE (t =6.54,4.88,respectively,both P ＜ 0.05).Among the patients with CU,the serum level of 25HVD was positively correlated with that of IFN-γ(r =0.738,P ＜ 0.05),but negatively correlated with that of IL-4 (r =-0.689,P ＜ 0.05),and uncorrelated with that of IgE (r =-0.271,P ＞ 0.05).Conclusion The serum level of 25HVD evidently decreased in patients with CU,and it may participate in the occurrence of CU by mediating the Th 1/Th2 imbalance.

Objective To summary and explore the indications,safety and effectiveness of no touch ostial cardioplegia delivery method during aortic valve replacement(AVR) for severe aortic insufficiency(AI),in order to provide guidance for clinical application.Methods Between September 2012 and February 2015,63 cases were randomly divided into two groups,in study group we used this technique in 31 patients with a mean age of(58.0 ± 15.0) years and a range of 31 to 74 years old.The degree of AI averaged (3.5 ± 0.5) according to a semiquantitative 5-grade scale[1].32 patients were enrolled in control group with a mean age of(60.4 ± 9.4) years and a range of 22 to 73 years old.The degree of AI averaged (3.6 ± 0.5).The abnormal aortic cusps were sutured though the aortic incision,which would be closed temporarily.Blood cardioplegia was infused repeatedly into the coronary osita through the closed aortic incision during the remainder of the procedure except AVR,when the closed aortic incision was reopened.Results In the study group,spontaneous resumption of nodal and normal sinus rhythm shortly after the removal of aortic cross-clamp was achieved in all patients,no hearts had to be electrically defibrillated and electrocardiogram showed no obvious S-T segment elevation.The time from cross-clamp removal to sinus rhythm was(12.3 ± 7.7) minutes in the study group,versus (18.6 ± 8.2) minutes in the control group(P =0.037).No operative mortality occurred in both groups.There was a significant increase in left ventricular ejection fraction[from(0.54 ± 0.09) preoperatively to(0.62 ± 0.06) postoperatively] (P =0.005) in patients of the study group,which was not seen in patients of the control group.Conclusion Preliminary results have shown that the no touch ostial cardioplegia delivery method is a feasible method for cardioplegia infusion during aortic valve replacement in patients with severe AI.

Objective To observe expression level of serum vascular endothelial growth factor-C (VEGF-C),VEGF-C receptor-2 and VEGF-C receptor-3 in patients with acute leukemia (AL) and to explore its clinical significance.Methods Enzyme-linked immuno sorbent assay (ELISA) was used to detect the serum expression levels of VEGF-C,VEGFR-2,and VEGFR-3 of 51 patients diagnosed with acute leukemia,43 patients under medical treatment and 16 healthy blood donors.Results (1) Serum VEGF-C,VEGFR-2,and VEGFR-3 expression levels in AL patients were significantly higher than those in normal control group.(2) Serum VEGF-C and VEGFR-2 expression levels in complete remission (CR) group significantly declined after treatment.Serum VEGF-C and VEGFR-2 expression levels in non-complete remission (NR) group slightly declined after treatment but no significant difference was found (P＞0.05).(3) No significant difference was found in serum VEGFR-3 expression levels both in CR group and NR group after treatment (P＞0.05).(4) Serum VEGF-C,VEGFR-2,and VEGFR-3 expression levels in NR group were significantly higher than those in CR group before treatment (P＜0.08).Conclusions Observing serum expression level of VEGF-C,VEGFR-2,and VEGFR-3 of AL patients may be helpful in monitoring curative effects and prognosis of acute leukemia.