Objective To study the effect of tubular stomach reconstruction on respiratory function and serum vascular endothelial growth factor(VEGF) level in patients with esophageal cancer.Methods 84 patients with esophagealcancer were randomIy divided into two groups.42 patients in the observation group were operated through tubular stomach reconstruction,while 42 patients in the control group were operated through full stomach and esophagus reconstruction of digestive tract.The respiratory function and serum VEGF were detected in all patients.Results The respiratory function was significantly declined after operation compared with before operation (P ＜ 0.05).The respiratory function was significantly improved postoperative two months compared with before operation (P ＜ 0.05,P ＜0.01),there was a significant difference between the two groups (P ＜ 0.05).Serum VEGF level was significantly decreased after operation in both two groups (P ＜ 0.0 1).Serum VEGF level of the observation group was significantly lower than that of the control group (P ＜ 0.05).Conclusion Tubular stomach reconstruction can improve respiratory function and prognosis of patients with esophageal cancer.

By adopting scoring method on the degree of senilization and methods of high cross frame labyrinthine experiment and shuttle box on the basis of systematic observation on behavior characteristics of rapid senilized dementia in SAM - P/8 model, the effect of acupuncture method for wakening the consciousness and opening of orifices was observed. Results showed SAM -P/8 revealed apparent senilized characteristics of brain, being in a low intelligent condition, which is a rather ideal model for the study of natural damentia in the elderly. The acupuncture methods revealed an action of delaying senility process, improving the condition of frightening and increasing the ability of learning and memory.

Objective To study carbapenem-resistant genes in a Myroides odoratimimus strain and clinical therapy.Methods A strain of Myroides odoratimimus was isolated from nephrostomy drainage fluid of a patient with urinary tract infection in Xinhua Hospital in May 2013.MicroflexTM MALDI-TOF MS and 16S rDNA sequencing were performed for strain identification.Vitek-2 Compact combined with E-test method was used for antibiotic susceptibility test.Modified Hodge test and imipenem/imipenem-inhibitor (IP/IPI)E-test were performed for drug-resistant phenotype screening.Carbapenemase genes blaMUS-1,blaVIM-1,blaVIM-2,blaIMP-1,blaIMP-2,blaNDM-1,blaOXA-48 and blaGESwere amplified by PCR,and the positive products were sequenced and analyzed.Results The isolated strain was identified as Myroides odoratimimus.The strain was resistant to 17 antibacterial agents,and the minimum inhibitory concentration (MIC) of imipenem was ≥ 16 μg/mL,while it was sensitive to minocycline (MIC =0.38 μg/mL) and intermediate to meropenem (MIC =6 μg/mL).It was negative in modified Hodge test but positive in IP/IPI E-test (IP/IPI≥ 8).blaMUS-1 gene was detected by PCR,and further confirmed by sequencing.Meropenem combined with minocycline was effective for the patient.Conclusion Carbapenem-resistance in this Myroides odoratimimus strain may be related to blaMUS-1 gene,and appropriate therapy for its infection should be based on the result of antibiotic susceptibility test.

Objective To investigate the prevalence and transmission route of Klebsiella pneumoniae carbapenemases ( KPC)-producing Klebsiella pneumoniae in Xinhua Hospital Affiliated to Shanghaijiao Tong University School of Medicine .Methods Carbapenem-resistant Klebsiella pneumoniae isolates from clinical samples were collected from Xinhua Hospital during January 2010 and December 2013.Vitek 2 Compact and disc diffusion method ( Kirby-Bauer method ) were used for identification of the strains and antibiotic susceptibility test . Modified Hodge test was performed for drug-resistant phenotype screening . Carbapenemase gene blaKPC was amplified by PCR, and the positive products were sequenced and analyzed . Enterobacterial repetitive intergenic consensus ( ERIC )-PCR was used to analyze molecular epidemiology of the KPC-producing strains .And clinical information of these isolates was analyzed .Results There were 77 carbapenem-resistant Klebsiella pneumoniae isolates in total , and 71 of them were positive in modified Hodge test.Sixty-nine isolates were identified carrying blaKPC-2 gene.All of the KPC-2-producing isolates were classified as the same genotype by ERIC-PCR. Among 12 patients with KPC-2-producing Klebsiella pneumoniae infection who were first identified in each departments , 7 were transferred from other departments, and 4 were treated in surgery intensive care unit (SICU).Conclusions Most of carbpenem-resistant Klebsiella pneumoniae strains isolated from Xinhua Hospital are KPC-2-producing .The outbreak of carbpenem-resistant Klebsiella pneumoniae infection in the hospital may be associated with the interdepartmental transfer of patients .

Objective:To study the HPLC fingerprint of Ningxinbao capsules, and establish a method for the simultaneous content determination of uracil, uridine, adenine and adenosine. Methods: The separation was carried out on an Agilent Zorbax SB-Aq C18 column(250 mm × 4. 6 mm, 5μm) with gradient elution using methanol-0. 05 mol·L-1 potassium dihydrogen phosphate at 30℃ and at a flow rate of 1. 0 ml·min-1 , the detection wavelength was 212 nm for fingerprint and 260 nm for the determination of the four nu-cleosines. Totally 10 batches of samples were analyzed with the developed HPLC fingerprint and the determination method, the data calculation was performed with similarity evaluation system in the chromatographic fingerprint of TCM. Results: In the fingerprint, 10 common peaks were marked and the separation of the four nucleosines was good. Conclusion:The method is simple and reliable. The HPLC fingerprint and contents of the four nucleosines in Ningxinbao capsules can be used for the quality control.

Objective To study the method and nursing measurements of the detection of portal venous pressure. Method The implantable drug delivery system was performed during the operation in 31 patients with liver cancer. The direct dynamic observation was performed by using the system and the corresponding nursing measurement was given. Result The measurement of portal venous pressure was successful performed 251 times in 31 cases with liver cancer and no complications were taken place. Conclusions The dynamic observation of portal venous of pressure by using the implantable drug delivery system was simple. Safety and could be repeated and could easily be receipted by the cases, the nurses skilled measured the key points of nursing and actively prevented the complications was taken implant role in increasing the rate of success of detection .

Objective To observe the changes of the skeletal development related cells after dihydroorotate dehydrogenase (DHODH) deficiency.Methods The DHODH expression in MC3T3-E1 cells derived from mouse calvaria osteoblast precursor cells was inhibited by specific small interfering RNAs (siRNAs),and cell proliferation,ATP production and expression levels of bone-related genes were investigated in these cells.Results After reducing the DHODH expression by using specific siRNAs,cell proliferation was inhibited and cell cycle was arrested at G1/S stage.In addition,the ATP production was reduced in whole cells,especially in mitochondria.Furthermore,the expression levels of Runt-related transcription factor 2 (Runx2) and osteocalcin (Ocn) mRNAs in the DHODH inhibition group were decreased compared with the control group.Conclusion Inhibiting DHODH protein affects the differentiation and maturation of osteoblasts.The mitochondrial dysfunction in osteoblasts may be one of causes leading to the abnormal bone formation in Miller syndrome.

Objective To investigate the mechanism of IL-1β in promoting glial scar formation after spinal cord injury.Methods The experimental model of SCI was created by extradural compression of the spinal cord using an aneurysm clip.Rats were randomly divided into model group, sham operation group, IL-1β inhibitor IL-1RA group, IL-1β group and IL-1β+JAK2-STAT3 inhibitor AG490 group, according to different interventions, then were given normal saline, IL-1RA, IL-1β and IL-1β+AG490 every 10 μL respectively, sham group received only laminectomy.The motion function of the hindlimbs of rats was measured by Basso Beattie Bresnahan(BBB) scores and the expression of GFAP, vimentin and p-STAT3 were detected by Western blot technique, immunofluorescence assay and immunohistochemistry technique at corresponding time points(at the 8th, 12th hour, 1st, 3rd, 7th and 14th day after SCI).Results The expression trend of p-STAT3(at the 8th and 12th hour after SCI),GFAP and vimentin(at the 7th and 14th day after SCI)was: the expressions of p-STAT3, GFAP and vimentin in the model group were significantly higher compared with the sham group(P<0.01), the expression of p-STAT3,GFAP andvimentin in the IL-1RA group were significantly lower compared with the model group(P<0.05) whereas significantly higher compared with the sham group(P<0.05);the expressions of p-STAT3, GFAP and vimentin in the IL-1β+AG490 group were significantly lower compared with the model group(P<0.05)whereas significantly higher compared with the sham group(P<0.05), the expressions of p-STAT3, GFAP and vimentin in the IL-1β group were significantly higher compared with the model group(P<0.05).Conclusions IL-1β can improve glial scar formation via JAK2-STAT3 signal.Inhibition of IL-1β or JAK2-STAT3 can reduce glial scar formation and promote functional recovery of spinal nerve.

Objective To determine whether fetal rats exposure to isoflurane will cause postnatal learning and memory deficits,and change Bcl-2/Bax ratio in the hippocampus CA1 and retrosplenial cortex in fetal brain of rats. Methods Twenty-eight Sprague Dawley pregnant rats at gestation day 21 (E21) were randomly divided into isoflurane treatment group(n=14) and sham control group(n=14). Rats in isoflurane treatment group were ex-posed to 1.3% isoflurane in a carrying gas of 30% oxygen, balance nitrogen for 6 h in a warmed, humidified cham-ber. For sham control group,animals were treated at the same condition with only carrying gas. In behavior study,the spatial learning and memory ability at juvenile ages was determined with the Morris Water Maze(MWM). In immunohistochemistry study,changes of Bcl-2 and Bax expression in hippocampus CA1 and retrosplenial cortex in the fetus brain after isoflurane treatment at 2 hours was performed by using immunofluorecence staining.Results In the MWM training, the escape latency to platform in the place trials showed no significant difference between the two groups,but the postnatal rats in 1.3% isoflurane group showed obviously improved retention of memory by spending more percentage of time swimming in the probe quadrant as compared to the control animals ((42.33±2.31) s vs (33.2±2.15) s, t=2.21, P<0.05) in the probe test. Compared to controls, 1.3% isoflu-rane exposure for 6 h to the pregnant rats increased the intensity of Bcl-2, decreased the intensity of Bax, and sig-nificantly increased the Bcl-2/Bax ratio in the fetal hippocampal CA1 region (4.40±0. 86 vs 1.31±0.32, t=3. 378, P<0.01) and the fetal retrosplenial cortex (5.07±1.27 vs 1.47±0.48, t=2.656, P < 0.05) respec-tively. Conclusion 1.3% isoflurane exposure in pregnant rats significantly improves the spatial retention memo-ry of their rat pups at a juvenile age and increases the Bcl-2/Bax ratio in the hippocampal CA1 region and the ret-resplenial cortex in the fetal rat brains.

OBJECTIVE:To establish the quality creteria of Dendrobium denneanum cultivated in Sichuan.METHODS: The reference substance had been isolated and identified.HPLC and TLC were applied for determination and identification respectively.RESULTS: The coumarin control had been successfully isolated and the TLC spots were clear.The linear range of coumarin was 0.096～0.480 ?g with an average recovery of 99.1%(RSD=1.44%,n=6).CONCLUSION: The results can overall reflect the internal quality of D.denneanum cultivated in Sichuan and provide basis for quality evaluation and development of D.denneanum from Sichuan.