Objective Calcium overload has important effect on cell injury in the ischemic stroke MR DWI/PWI mismatch may display the ischemic penumbra We try to explain the relationship between the characters of MR DWI/PWI and intracellular Ca 2+ in the different ischemic region through the model of focal cerebral ischemia Methods Twenty-eight rabbits underwent permanent middle cerebral artery occlusion (MCAo) From 0 5 h to 36 h after the onset of occlusion, diffusion-, perfusion-, and T 2-weighted MR imaging were performed to generate ADC, CBF, CBV parameters and anatomic images After the MR scanning, the same brain slices were incubated with Fluo-3/AM followed by the examination using laser confocal scanning microscope (LCSM) At different ischemic time, fluorescence intensity (FI) of the intracellular Ca 2+ in the DWI/PWI match region and mismatch one was recorded FI match /FI contralateral and FI mismatch /FI contralateral were produced and analyzed Results (1) MR outcome: The DWI/PWI match was located in the lateral caudoputamen and the DWI/PWI mismatch was mainly located in the frontoparietal cortex At 0 5 h after MCAo, CBF and CBV of lateral caudoputamen and frontoparietal cortex in the affected side deceased marked and the rCBF and rCBV of lateral caudoputamen were less then that of frontoparietal cortex in all groups ( t =-11 74, P

The antioxidative capability and Na+-K+-ATPase α1-subunit mRNA expression in myocardium of hypothyroid rats induced by low-iodine diet were observed. The results showed that the antioxidative capability of myocardium decreased, resulting in lipid peroxidative damage, atrophy of myocardial cells and chondrification of tunica intima, along with decreased expression of sodium pump α1 subunit mRNA in hypothyroid rats.

Objective To analyze the clinical features and validation of Brighton criteria in Guillain-Barré syndrome (GBS) patients from southern China.Methods The clinical data of hospitalized GBS patients from 69 hospitals of 14 provinces/cities in southern China,the area south of the Huaihe River,between 1 January 2013 and 30 September 2016,were collected and analyzed retrospectively,and patients were classified according to the Brighton criteria of case definition,ranging from a highest (defined as level one) to a lowest (level four) level of diagnostic certainty.Results A total of 1 358 GBS patients were collected,including 51 cases with cranial nerve variants,157 with Miler-Fisher syndrome and 1 150 with classic GBS characterized by flaccid weakness of limbs.Among 1 150 cases of classic GBS,49.57％ (570/1 150) patients had antecedent events,with respiratory infection predominated (71.23％,406/570);83.74％ (963/1 150) presented limb weakness at onset,99.21％ (1 124/1 133) reached the peak within four weeks,with a score of 3.15 ± 1.16 for Hughes Disability Scale;99.56％ (1 128/1 133)developed bilateral weakness and 95.39％ (1 097/1 150) manifested flexia or hyporeflexia;the cerebrospinal fluid showed albuminocytologic dissociation in 80.58％ (772/958) patients whose lumbar puncture was performed;demyelinating GBS accounted for 48.14％ (401/833) and axonal subtype 18.01％ (150/833) respectively in patients with findings of nerve conduction studies available.According to Brighton criteria,the patients were stratified as level one in 44.09％ (507/1 150),level two in 45.74％ (526/1 150),level three in 7.57％ (87/1 150) and level four in 2.61％ (30/1 150) of all the patients,and 69.55％ (507/729),28.67％ (209/729),0％ (0/729) and 1.78％ (13/729),respectively in the patients with complete data (n =729).Conclusions In southern China,demyelinating subtype of GBS is predominant,whereas the proportion of axonal subtype is remarkably lower than that in northern China.The Brighton criteria have a high sensitivity for the diagnosis of GBS in southern China,and examination of cerebrospinal fluid and electrodiagnostic studies are necessary for stratified diagnosis.