1.Engraftment syndrome after allogeneic hematopoietic stem cell transplantation: a case report and literature review
Feng NING ; Jingwen WANG ; Lei YANG ; Xin LI
Journal of Leukemia & Lymphoma 2009;18(7):415-416,418
Objective To study the engraftment syndrome (ES) and the possible etiology after haploidentical allognneic hematopoietic stem cell transplantation (al]o-HSCT). Methods The clinical manifestation, treatment and outcome of a chronic myeloid leukemia patient who after allo-HSCT were presented. Results The patient started to cough on day 2 after allo-HSCT, dry cough at beginning, white foam sputum, non-infectious fever, fluid retention, weight gain, transient encephalopathy on day 4. The clinical symptoms were improved after treatment with corticosteroids, in which ES was diagnosed. Conclusion ES is a series of clinical syndromes following transplantation which is different from acute graft versus host disease. It occurred after HSCT, and corticosteroids are often effective. Therefore early recognition and accurate diagnosis is very important.
2.Resveratrol inhibits high glucose-induced hypertrophy in myocardial cell line H9C2
Xiao WEI ; Jianming LEI ; Jingwen GUO ; Hua XIAO
Basic & Clinical Medicine 2017;37(3):320-324
Objective To investigate the effect of resveratrol on high glucose-induced oxidative stress and cardiomyo-cyte hypertrophy and possible mechanism .Methods H9C2 cardiomyocytes were divided into normal glucose group , high glucose group , resveratrol group and resveratrol +DAPT group respectively .The cell surface was measured by rhodamine-labeled phalloidin .Reactive oxygen species generation was measured using DCFH-DA.The contents of SOD and MDA were evaluated by colorimetry .The mRNA and protein levels of Notch 1, Hes1, ANP and BNP were evaluated by RT-qPCR and western blot .Results High glucose exposure significantly increased cell surface area ( P<0.01) and enhanced ROS and MDA generation ( P <0.01 ) with concomitant decrease of SOD activity ( P <0.01).It also decreased the mRNA and protein expressions of Notch 1 and Hes1(P<0.05), and increased the ex-pression levels of ANP and BNP ( P<0.05 ) .Resveratrol treatment inhibited cell surface expanding ( P<0.01 ) and decreased ROS and MDA generation (P<0.01) with concomitant enhance SOD activity (P<0.01), as well as in-creased the expression level of Notch 1 and Hes1 ( P<0.05 ) , and decreased the expression of ANP and BNP ( P<0.05).Conclusions Resveratrol may inhibit high glucose induced H9C2 cardiomyocytes hypertrophy by improving oxidative stress and activating Notch 1/Hes1 signaling pathway .
3.An information management system of scientific research funds to be established and applied
Xiangyu WANG ; Lei QIAN ; Ping PING ; Jingwen DUAN ; Hong JIANG
Chinese Journal of Medical Science Research Management 2015;28(4):334-336
Following the increasing of amount and improving of management year by year,the management of scientific research funds must be informationized.We established an information management system of scientific research funds,according to the management standards of different departments which were the sources of funds.The system including two main modules.:budget management and expenditure management.The main functions of the system as follows:establishing the budget,expensing according to the budget,inquiring the feedback data,etc.The system increased the accuracy and effectiveness of management of scientific research funds.
4.Changes of aryl hydrocarbon receptor in cardiac hypertrophy induced by high glucose in vitro
Xuejiao TANG ; Hua XIAO ; Lei ZHANG ; Xiao WEI ; Jianming LEI ; Jingwen GUO
Chinese Journal of Pathophysiology 2016;32(10):1744-1749
AIM:To investigate the changes of aryl hydrocarbon receptor (AhR) in the process of cardiomyo-cyte hypertrophy induced by high glucose , and to explore its potential mechanisms .METHODS: The rat cardiomyocytes (H9c2 cells) were divided into normal glucose group , high glucose group, DMSO group and resveratrol (an AhR antago-nist) group.The content and distribution of AhR were observed with immunofluorescence staining .The myocardial cells were stained with rhodamine-labeled phalloidin to visualize cytoskeleton , and the cell surface area were determined after im-aging by fluorescence microscopy .The generation of reactive oxygen species ( ROS) in the cardiomyocytes was measured u-sing a fluorescent probe DCFH-DA.The mRNA expression of AhR , CYP1A1, atrial natriuretic peptide ( ANP) and brain natriuretic peptide ( BNP) were evaluated by real-time quantitative PCR ( RT-qPCR).The protein levels of AhR, CYP1A1, ANP and BNP were assessed by Western blot .RESULTS:AhR was constitutively presented in the cytosol un-der normal-glucose condition and was translocated to the nuclei under high-glucose condition .High glucose induced cardiac hypertrophy , and increased ROS generation .Significant reductions in the cell size and ROS generation were observed after treated with resveratrol.The expression of AhR, CYP1A1, ANP and BNP at mRNA and protein levels in high glucose group was increased as compared with normal glucose group and resveratrol group , and the above-mentioned indexes signifi-cantly decreased in resveratrol group as compared with DMSO group .CONCLUSION: High glucose-induced cardiac hy-pertrophy increases AhR expression , which may be involved in the maintenance of glucose homeostasis in the cardiomyo-cytes.AhR translocation to the nucleus induced by high glucose results in the increases in CYP 1A1 expression and ROS generation, which may be an important mechanism of high glucose-induced cardiomyocyte hypertrophy .
5.Prognostic analysis of allogeneic hematopoietic stem cell transplantation in treating patients with malignant hematological diseases
Feng NING ; Jingwen WANG ; Lei YANG ; Jing CUI ; Yu LI ; Xin LI ; Xiuxiu YIN
Journal of Leukemia & Lymphoma 2010;19(6):338-340
Objective To explore the clinical related prognostic factors of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in treating malignant hematological diseases. Methods From September 1997 to August 2008,a total of 26 patients with hematological diseases were treated with allo-HSCT from HLA identical-sibling and haplo-identical donors in our hospital, including 14 patients with acute leukemias,10 with chronic myeloid leukemias,and 2 myelodysplastic syndromes. Results All patients achieved sustained full donor type engraftment. The cumulative overall survival (OS) was 63.9 %,and cumulative disease free survival (DFS) was 62.6 %. Fifteen patients had graft-versus-host disease (GVHD) (57.7 %),including 8 acute GVHD(aGVHD) (30.8 %) (grade Ⅲ-Ⅳ aGVHD was 15.4 %) and 7 chronic GVHD. GVHD between HLA identical-sibling and haplo-identical donors was different and there was statistic difference between the two groups (P=0.014). 4 patients relapsed,7 patients died. The univariate analysis showed OS were correlated with grade Ⅳ aGVHD (P=0.05) and CMV infection (P=0.027). Conclusion Allo-HSCT is effective for the cure of patients with malignant hematological diseases. The key to improve the efficacy of HSCT is to reduce the incidence of transplant-related complications,especially GVHD and infection.
6.Application of Problem-based Learning Mode in Clinical Pharmacist Teacher Training
Lei WANG ; Jingwen WANG ; Wei ZHANG ; Yan ZHANG ; Yi QIAO ; Aidong WEN
China Pharmacist 2016;19(5):1003-1005
Objective:To evaluate the effects of problem-based learning(PBL)mode in the clinical pharmacist teacher training. Methods:The specific implementation content,process and organizational methods of PBL mode were introduced in the clinical pharmacist teacher training. Results:Through the scientific analysis on the student marks,the results showed that the theoretical test scores of the students were increased by average 30 points when compared with those before the training,and the average score of clinical practice was more than 85 points. The achievement fully reflected the significant effect of PBL teaching courses and the reasonable design of teaching plan. Conclusion:The first application of PBL mode in the clinical pharmacist teacher training was welcomed by all the students. The teaching method not only improves the purpose and funny of learning,but also allows students to explore a targeted manner and apply theoretical knowledge in order to improve the analysis and problem-solving skills,and obtain more systematic and solid theoretical knowledge to closely integrate with the clinical needs, therefore,PBL mode is worthy of promotion.
7.Research progresses in cancer stem cell and its vascular microenvironment.
Jingwen WANG ; Lei DENG ; You LU
Journal of Biomedical Engineering 2013;30(3):675-678
Cancer stem cells (CSCs) and angiogenesis play important roles in generation and development of malignant tumours. The number of researches concerned both of them is increasing rapidly and many impressive conclusions have been achieved based on recent studies. It is indicated that the CSCs have complicated interaction with the adjacent vascular microenvironment and they act on the disease progression together. CSCs may enhance angiogenesis while the vascular microenvironment has effects on maintenance and even induction of stemness, and new illustrations of mechanisms are constantly obtained. In this review, we summarize the current research status of mutual actions between CSCs and the vascular microenvironment, and also overview the latest progresses about relevant targeted therapies, to provide advisable information for future preclinical and clinical explorations.
Humans
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Neoplasms
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blood supply
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pathology
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Neoplastic Stem Cells
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pathology
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physiology
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Neovascularization, Pathologic
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pathology
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physiopathology
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Tumor Microenvironment
8.Role of ROS/PKC/p38 MAPK pathway in protective effects of polysac-charide from Fructus corni on rat cardiomyocytes against hypoxia-reoxy-genation injury
Lina LAI ; Lihua SONG ; Xiaojing ZHANG ; Xiaoyi ZHANG ; Jingwen LEI ; Fang LIU ; Chunhua GUO ; Xiaoliang SONG
Chinese Journal of Pathophysiology 2014;(12):2201-2205
[ ABSTRACT] AIM:To investigate the effect of polysaccharide from Fructus corni ( PFC) on cardiomyocytes against hypoxia/reoxygenation ( H/R) injury and its possible relationship with ROS/PKC/p38 MAPK pathway.METHODS:Prima-ry cardiomyocytes were isolated from neonatal SD rats and randomly divided into normal group, H/R group, PFC (20 mg/L, 100 mg/L and 200 mg/L) preconditioning+H/R groups, chelerythrine+PFC (100 mg/L)+H/R group and SB203580+PFC (100 mg/L)+H/R group.The cell viability was measured by inverted microscopic observation.Apoptosis in the car-diomyocytes was detected by Hoechst 33258 staining and fluorescence microscopy.The levels of lactate dehydrogenase ( LDH) and superoxide dismutase ( SOD) in the cell culture supernatants, and the reactive oxygen species ( ROS) in the cells were also measured by microplate reader.The protein levels of PKC, p-p38 MAPK and HSP70 in the cells were detec-ted by Western blotting.RESULTS:Compared with normal group, the cell viability and beating frequency were decreased in H/R group.LDH and ROS contents, apoptotic rate and p-p38 MAPK level increased significantly (P<0.01).Compared with H/R group, PFC preconditioning increased beating frequency, SOD activity and the protein level of PKC and HSP70, and decreased ROS production, the protein level of p-p38 MAPK and cell apoptotic rate.However, the effect of PFC was in-hibited by chelerythrine or SB203580.CONCLUSION:PFC may protect cardiomyocytes from hypoxia/reoxygenation injury. Its mechanism is possibly involved in the inhibition of ROS via increasing the activity of SOD and the activation of PKC, and suppression of excessive activation of p38 MAPK.
9.An in vitro mineralization study of hydrogen existence form and surface bioactivity of titanium
Xi LIN ; Lei ZHOU ; Xianglong DING ; Fang JIA ; Weizhen LIU ; Jingwen DING
Journal of Practical Stomatology 2014;(4):451-455
Objective:To investigate the relationship between surface hydrogen form and the bioactivity of titanium.Methods:Sandblast titanium was etched with the combination of sulfuric and hydrochloric acids(SLA group,n=3 ).Then etched titanium was heat at 450 ℃in air(SLA+HT group,n=3).Surface topography,roughness,hydrophility,surface chemical texture were observed. Finally,the titanium samples were soaked in body simulate fluid for 3 days,the mineral deposition properties were observed by X-ray diffraction.Results:Titanium hydride was formed on the titanium surface after etching.After heat treatment,surface texture and roughness were not changed,titanium hydride decomposed and hydrophility increased.More hydroxyapatite was found on the surface of the samples treated by SLA+HT and followed by SBF.Conclusion:Titanium hydride can not improve the bioactivity of titanium, heat treatment may increase the mineralization.
10.Patter and Method of Teacher Training for Clinical Pharmacists in Troops
Wei ZHANG ; Jingwen WANG ; Wei ZHANG ; Tingting FAN ; Mingming WANG ; Lei WANG
China Pharmacist 2015;(6):1049-1051
Objective:To explore the teaching mode and method of teacher training for clinical pharmacists in troops. Methods:Combined with the scheme and requirements of teacher training for clinical pharmacists issued by the Ministry of Health, the training mode and scheme with military characteristics were explored, and the points of innovation and experience were summarized. Results:The teacher training for clinical pharmacists in troops was different from that in the local, which should highlight the characteristics of military management and a full range of teaching. The health department of general logistics was responsible for the overall arrange-ment, multi-center and multi-department cooperated closely, and clinical pharmacy center implemented the project. With the improve-ment of clinical teaching skills as the purpose and the new PBL as the teaching mode, the entire military management was carried out, and the teaching plan and content suitable for the teacher training in troops were drawn up. Conclusion:According to the results of the 9-week comprehensive teacher training practice, it is proved that the scheme is effective and has achieved initial results, which lay good foundation for the future development of clinical pharmacist work and explore the new ideas and methods for the clinical pharmacist training. However, the project still needs further improvement.