Ubiquitylation is a highly versatile post-translation modification that controls virtually all types of cellular events by changing the molecular landscape,the three-dimensional structure,the location or activity of the substrate,influencing the interactions of substrate with other proteins.Many cellular activities of ubiquitin and its functions are deciphered by specific ubiquitin binding domains (UBDs).UBD interacts with the different type of Ubiquitylation with a variety of lengths and linkage patterns based on the specific functional surfaces.So far over twenty distinct UBD families have been identified,and the known members of this group have expanded rapidly.The UBD families,the decoder of ubiquitylation network,are build-ing a foundation for in-depth understanding the ubiquitination proteomics “ubiquitome”.

Objective To investigate the effect of cobalt chromium alloy onlay on the direct repair of cracked vital pulp teeth with the resin compostie filling and porcelain-fused-to-metal (PFM ) crown repair as the control .Methods 201 cracked vital pulp teeth from 201 cases were randomly divided into 3 groups according to the clinical symptoms :mild discomfort at occlusion (group A) ,pain at occlusion accompanied with transient pain to cold or hot stimulation (group B) and pain at occlusion accompanied with continuous pain to cold or hot stimulation(group C) .The cracked teeth directly were restored by the resin composite filling ,cobalt chromium alloy onlay and PFM crown repair and the effective rate in each group was observed after 2-year follow up .Results The effective rate of the group A ,B and C were statistically significant different from each other (P<0 .05) .The effective rate of the co-balt chromium alloy onlay and the PFM crown was statistically significant higher than that of resin composite (P<0 .05) .Conclusion The direct repair with cobalt chromium alloy onlay is an effective repair method for the cracked vital pulp teeth with mild discomfort at occlusion and mild pain accompanied with transient pain to cold or hot stimulation .

BACKGROUND:Distal femoral fractures are mainly treated with less invasive stabilization system (LISS) plate or retrograde interlocking intramedul ary nail fixation, but choosing which method is controversial, and studies on their biomechanical properties are few. OBJECTIVE:To compare the biomechanical stability of retrograde interlocking intramedul ary nail and LISS plate fixation in the treatment of distal femoral fractures. METHODS:Twelve male cadaveric femurs were col ected, and the injured, with abnormal bone density and osteonosus specimens were excluded through X-ray examination, fol owed by randomly divided into two groups. Models of AO type A3 supracondylar fracture were prepared, and were fixed with LISS plate and retrograde interlocking intramedul ary nail, respectively. The compressive stiffness and displacement values under axial compression and loading of 100, 300 and 500 N, as wel as the bending strength of the specimens under bending load were observed. RESULTS AND CONCLUSION:(1) The resistance to axial deformation capacity (compressive stiffness) of the LISS plate was superior to the retrograde interlocking intramedul ary nail (P<0.05);while the resistance to bending deformation capacity (flexural strength) of the retrograde interlocking intramedul ary nail was not significantly greater than that of the LISS plate (P>0.05). (2) Under axial compressive loading of 100, 300 and 500 N, the displacement values of LISS plate were significantly less than those of the retrograde interlocking intramedul ary nail (P<0.05). (3) In conclusion, in the distal femoral fracture fixation, the stiffness of the retrograde interlocking intramedul ary nail is low. While the LISS plate not only has certain deformation, but also has strong rigidity with firm internal fixation, which provides excel ent biological environment for fracture healing;thus it is a reliable treatment for distal femoral fractures.

Objective To evaluate the long term curative effects of radiotherapy combined with photodynamic therapy on the advanced esophageal cancer.Methods From Oct 1991 to Dec 1992, 60 patients with advanced esophageal cancer were randomly divided into two groups, 30 patients received conventional radiotherapy alone (CRT), the other 30 cases received radiotherapy combined with photodynamic therapy (PRT). After PRT the total dose of 40Gy,for 4 weeks the hematoporphyrin derivate(HpD) was given using 5mg/kg body intravenously and after 48 and 72 hours,the location of lesion were irradiation by 630-nm red light with 400～500mW/cm 2. Laser irradiation was given for 15 minutes to each part of tumor.Results The 5,10-year survival rates were 16 7% ,10 0% respetively;for CRT group and 29 9%, 16 7% respectively; for PRT group, The difference was significant (P

0 05).The survival rate of 1,2,3-year of SG and CRG respectively were 72 58%,51 61% and 37 10%,and 58 10%,32 26% and 20 97%.There was significant difference in the survival rate of 2,3-year between two groups(P

Less consciousness for science research,lower level of research work,shortage of research team,lack of research institution and so on are the mean problems in clinical hospitals.In recent years,we take the measures of carrying out research training,perfecting research equipment,strengthening the research team,establishing encourage and punish rules.these measures promote the research work of our hospital,change the research sense of hospital staff therefor we get notable progress in research work.

Medical research is the core-competitiveness of hospitals.Research management in hospitals with weak scientific research capacity is different from others and has its own demands.People of the management neet to develop themselves to meet the special demands,and can then help develop the hospital.

In hospitals,the construction and development of key academic disciplines is the fundamental driving force for the ongoing improvement of healthcare competence and scientific research capacity.This paper draw on the experience of a recent 3-year disciplinary development program of a comprehensive hospital in Shanxi province,discussed measures that has been proven effective and set up guide line for future advancement of disciplinary development.

OBJECTIVE:To investigate the effects of rosuvastatin combined with berberine hydrochloride on related indexes in patients with acute cerebral infarction.METHODS:Medical information of 120 patients diagnosed as acute ischemic cerebral infarction were analyzed retrospectively,and they divided into observation group (65 cases) and control group (55 cases) according to route of administration.Both groups were given routine treatment;control group was additionally given Rosuvastatin calcium tablets 10 mg orally before bedtime,once a day;observation group was additionally given Berberine hydrochloride tablets 0.3 g orally,3 times a day,on the basis of control group.Treatment courses of 2 groups lasted for 14 d.The levels of TC,TG,LDL-C,HDL-C,hs-CRP,ox-LDL,NIHSS score,ADL score,TPA,IMT,Crouse score,the number of unstable plaque and ADR were observed and compared between 2 groups before and after treatment.RESULTS:Before treatment,there was no statistical significance in above indexes between 2 groups (P＞0.05).After treatment,the serum levels ofTC,TG,LDL-C,hs-CRP and ox-LDL in 2 groups were significantly lower than before,and the observation group was significantly lower than the control group,with statistical significance (P＜0.05).After treatment,NIHSS score of 2 groups were significantly lower than before,and ADL score,HDL-C levels were significantly higher than before,with statistical significance (P＜0.05);there was no statistical significance between 2 groups (P＞0.05).After treatment,there was no statistical significance in IMT,Crouse score and the number of unstable plaque in control group compared to before treatment (P＞0.05),but in observation group were significantly lower than before treatment,and also lower than control group at corresponding period,with statistical significance (P＜0.05).There was no statistical significance in TPA between 2 groups before and after treatment(P＞0.05).There was no statistical significance in the indcidence of ADR between 2 groups during treatment(P＞0.05).CONCLUSIONS:Based on routine treatment,rosuvastatin combined with berberine hydrochloride could effectively reduce blood lipid levels in patients with acute cerebral infarction,stabilize or reverse atherosclerotic plaque,and reduce inflammation and oxidative stress with good safety.

Aim To investigate the pharmacokinetics of dipfluzine hydrochloride,a novel piperazines calcium antagonist.Methods Eighteen Beagle dogs were randomly divided into three groups,which were administered with dipfluzine hydrochloride at iv single dose of 1.5,3.0 and 6.0 mg?kg-1,respectively.The blood was collected at different time.A RP-HPLC method was developed to determine the concentration of dipfluzine hydrochloride in plasma.The pharmacokinetic parameters were calculated by 3P97 software.Results The specificity,lowest limit of detection and quantification,extraction recoveries,the precision of intra-and inter-day and stability were qualified to the pharmacokinetic study.The concentration-time courses of dipfluzine hydrochloride were best fitted to a two-compartment open model at three doses.The main pharmacokinetic parameters at three doses were 24.7,24.2 and 29.6 h for T12?,0.44,1.12 and 2.86 g?min?L-1 for AUC,1.30,1.22 and 1.28 L?kg-1 for Vc,and 3.4?10-3,2.7?10-3 and 2.1?10-3 L?kg-1?min-1 for CL,respectively.Conclusions The developed RP-HPLC method for determination of dipfluzine hydrochloride in plasma can satisfy the requirement of pharmacokinetic study after iv dipfluzine hydrochloride.Analysis of plasma concentration-time curves indicates a biphasic decrease.There was a linear relationship between AUC and dosage.