Objective To observe the efficacy and safety of safflower yellow injection in the treatment of sta-ble angina pectoris.Methods 70 patients with stable angina pectoris were selected and divided into observation group and control group by random number table method,35 cases in each group.The observation group used injection of safflower,and the control group used Xuesaitong.The effect was compared between the two groups.Results The total effective rate of the observation group was 94.3%,which was higher than 74.3% of the control group,the differ-ence was statistically significant(χ2 =5.285,P <0.01).The clinical indexes of the two groups after treatment were significantly improved than before treatment,the differences were statistically significant (t =13.021,25.407,3.376, 3.930,7.119,10.163,18.044,3.765,3.124,6.096,all P <0.05).The angina attack times of the observation group was less than that of the control group ,the dosage of nitroglycerin was lower than that of the control group ,the differences were statistically significant(t =4.686,6.568,all P <0.05).The incidence rate of adverse reaction was 5.7% in the observation group,compared with the control group of 8.6%,the difference was not statistically signifi-cant(χ2 =0.729,P >0.05).Conclusion The injection of safflower yellow pigment in the treatment of stable angina pectoris has high safety.

Objective To study the effects of hypoxia on repair of radiation damage and the possible mechanism. Methods Rat jejunal epithelium cell was taken as study object and divided into control group, irradiation group and irradiation-hypoxia group. The irradiation group was irradiated only once by 60 Co-? ray at the dose of 12 Gy and the irradiation-hypoxia group was treated with hypoxia (5% oxygen) for 6 h after irradiation. MTT was used to detect the survival rate of the irradiated cells, 3H-TdR incorporation was used to detect cell proliferation, and unscheduled DNA synthesis and single cell gel electrophoresis were used to detect DNA repair and flow cytometry was used to detect cell cycle. Results As compared with the irradiation group, the cells in the irradiation-hypoxia group repaired rapidly, proliferated obviously and the survival rate was increased about 10% (P

Objective To investigate the preventive effect of psychological intervention combined with cod liver oil ointment on radiation nasal inflammation in nasopharyngeal carcinoma patients. Methods 90 patients with nasopharyngeal carcinoma treated in our hospital from January 2015 to December 2016 were randomly divided into study group and control group. The control group was treated with nasopharyngeal irrigation. The study group was given psychological intervention combined with cod liver oil ointment. Results The treatment effect of the study group was significantly better than that of the control group, and the incidence of radiation rhinitis was significantly lower than that of the control group. There was no significant difference between the two groups before the endoscopic score, the CT score of the sinus and the cilia transmission rate Improved, the study group was significantly better than the control group(P<0.05); before treatment, the two groups of patients with no significant differences in clinical symptoms, after treatment have been improved, the study group was significantly better than the control group(P<0.05). Conclusion The application of psychological intervention combined with cod liver oil ointment in radiotherapy of nasopharyngeal carcinoma is beneficial to prevent radiation rhinitis and improve the function of sinus function.

Obejective To evaluate the accuracies of ten commercial total 25-hydroxyvitamin D [25(OH) D] immnoassays.Methods NIST 25 (OH) D reference material SRM 972a,which consisted of four vials of frozen serum with different concentration levels of different 25 (OH) D types,and two human serumsamples provided by our lab (BIMT),which had different concentration levels of 25 (OH) D3,were analyzed by ten total 25-hydroxyvitamin D immnoassays from Biomerieux,Mindray,Maccura,Chemclin,Abbott (2),Siemens,SNIBE (2),Roche,and by isotope-dilution liquid chromatography-tandem mass spectrometry(ID-LC/MS/MS) founded by BIMT.For the measurements of SRM 972a,the biases between tested values and certified values were calculated as a evaluating indicator,meanwhile the test biases between immnoassays and ID-LC/MS/MS were used as a evaluating indicator for the measurements of BIMT 25(OH) D3 serum samples.Test bias lower than 10％ was deemed acceptible.Results The ID-LC/MS/MS exhibited low biases at (1.6％-2.8％) in the measurements of all levels of SRM 972a.8 immnoassays showed low biases at(1.5％-3.5％) in the measurements of level 1 of SRM 972a,which had a high 25(OH) D3 concentration level,but only 2 immnoassays gave low biases at (3.6％-3.7％)in the measurements of high 25 (OH)D2 concentration sample (level 3).While,5 immnoassays gave low biases at (-0.3％-9.0％) in the measurements of high 3-epi-25 (OH) D3 concentrationsample (level 4).It seems that,when SRM 972a were analyzed,only one of the ten commercial total 25 (OH)D immnoassays were in good accuracy and analytical specificity agrements with ID-LC/MS/MS.When two human serum25(OH) D3samples from BIMT were tested,most immunoassays were overall in relative good agreement with ID-LC/MS/MS at high 25 (OH) D3concentration level.Conclusion The test biases in the total 25 (OH) D measurements are differences between differentimmnoassays and ID-LC/MS/MS,and the specificities of current commercial total 25 (OH) D immnoassays should be improved,especially the performance on the equivalent recognition of 25 (OH) D2 and 25 (OH) D3.

Objective To testify the lung injury induced by cardiopulmonary bypass(CPB) in canine model and observe the influence of CPB on the aquaporin-1 ( AQP1 ) mRNA expression in canine lung. Methods 8 mongrel dogs were used to perform the cardiopulmonary bypass. The hearts arrested for 90 minutes with mild hypothermia and rebeated for 6 hours. The hemodynamics, the ratio of lung dry weight and wet weight, the plasmic osmotic pressure, and the characteristics of light and fine structure were analyzed. The retro-transcription polyase chain reaction ( RT-PCR ) was used to measure the expression of AQP1 mRNA during the CPB. Results The hemodynamic data were stable in different time point during the CPB (P ＞0.05 ). The ratio of lung dry weight and wet weight was getting lower ( P ＜0.001) and the plasmic osmotic pressure was getting higher due to the prolongation of the CPB time and reperfusion time ( P ＜0.01). The light and electron microscopy showed the prominent aggregation of the white blood cell, severe interstitial edema and mild tear of respiratory membrane after 3 hour and 6-hour rebeat. AQP1 mRNA expression in lung was downregulated, 78.4% after 3-hour reperfusion and 55.5% after 6-hour reperfusion respectively, comparing to the level before CPB. Conclusion We recognize that the lung injury and lung edema were severe following 3-hour and 6-hour rebeat in CPB and hypothesize that the down-regulation of lung AQP1 mRNA expression may be a sign of pulmonary interstitial capillary injury induced by CPB.

Objective:To examine the reliability and validity of the telephone version of Information-Memory-Concentration Test (T-IMCT) in screening dementia when administered by telephone To evaluate the relation of the face-to-face and the telephonic interview for assessing the cognitive status Methods:T-IMCT was adapted from Information-Memory-Concentration test for the telephonic interview 65 subjects with dementia and 67 normal control subjects were examined by an experienced clinician, including clinical, neuropsychological and instrumental examination (CT or MRI) T-IMCT was administrated by telephone after 2 weeks, comparing the score from face-to-face interview with telephonic interview 20 subjects were administered the test twice by the same doctor to measure the test-retest reliability, and another 20 subjects were administered the test by two doctors to measure the inter-rater reliability Informants supervised the subjects to insure the authenticity of assessment administered by telephone Results:T-IMCT achieved a sensitivity of 80% and specificity of 80 6% in differentiating demented from healthy control subjects There was a high correlation between face-to-face interview and telephonic interview, Pearson’s correlation coefficient was 0 92 (P

Aquaporins (AQPs) is a novel family of membrane protein which is in charge of the transportation of water across the membranes. It has been testified that AQPs may be related to many physiological functions and pathophysiological disorders. In lung, 6 subtypes of AQPs were found, 4 of which have been located in certain tissue. AQP1, AQP3, AQP4 and AQP5 are located in upper respiratory tract and may contribute to the mucus production, bronchial moisturizing and gland secretion. AQP1 and AQP5 are located in lower respiratory tract and may participate in directing the water movement across the blood-gas barrier. AQPs in natal lung may be an important factor to accelerate the reabsorption of the lung liquid. In adult, some diseases such as asthma, pulmonary edema, adult respiratory distress syndrome, which have been considered unbalanced water movement, may be also concerned with AQPs. The relationship between lung edema and AQPs have not been elucidated in details. It should be verified how AQPs work in more organs and tissues as well as in some diseases.

Objective To explore the effect of the combined therapy on tension-type headache after acute brain trauma.Methods Fourteen patients with mild brain trauma and complained severe headache diagnosed as tension-type headache were treated by nerve and pain points block with a mixture of 0.4% lidocaine, triamcinolone acetonide 10 mg accompanied by celecoxib and eperisone. After the block, the degree of pain severity and the duration of headache were observed and recorded. The therapeutic effects were evaluated with visual analogue scale (VAS).Results There was a significant decrease in the degree of pain severity and the duration of headache after treatment ( P<0.01).Conclusion The combined therapy mainly including nerve block has a significant effect on tension-type headache after acute brain trauma.

Objective To discuss the application value of the capillary electrophoresis in detecting the glycosylation hemoglobin (HbA1c)level.Methods By utilizing the uncoated fused silica capillary column(30 cm in length and 25 μm in diameter)and the specific adsorption peak,the HbA1c levels in the healthy people,patients with diabetes mellitus(DM)and high risk people were de-tected by the photodiode array(PDA)method at the wavelength of 415 nm.During the research process,the precision and accuracy were perfomred the accuracy analysis.Results HbA1c could be effectively separated from non-HbA1c,with a sharp peak.And the relative quantity of HbA1c could be determined.Conclusion The capillary electrophoresis is characterized by specificity,high precision,and high accuracy.Therefore,it should be widely applied in clinic.

Purpose　The aim is to obtain the cDNA sequence of encoding extramembrane human FL gene with high level expression in E.coli. Methods　The primers were designed based on the known FL cDNA sequence. The total RNA was isolated from fetal liver cells , and then RT-PCR was performed. The fragment was cloned into pUC-18T vector, and further sequenced by automatic sequence analyzer. The gene was inserted into GST fusion expression vector between BamH Ⅰ and EcoR Ⅰ sites. The recombinant plasmid was transformed into E.coli strain DH5 α and induced with 1mmol/L IPTG.Results　The 546bp DNA fragment was amplified by RT-PCR method from fetal liver cells and its sequence was identical to the published sequence encoding human FL. The expressed fusion protein, with molecular weight of about 22kD, was about 10% of the total bacteria protein by SDS-PAGE and densitometry analysis.Conclusion　cDNA was cloned successfully. This study provided a basis for the further fundamental research and clinical application of FL.