1.Diagnosis and treatment of blunt renal injuryin children
Yi LIU ; Xiangtie LI ; Aimin ZHANG ; Jianzhong CHEN ; Jingqin LI
Chinese Journal of Trauma 2008;24(9):733-735
Objective To summarize the characteristics of blunt renal injury in children and re-port experiences in its diagnosis and treatment. Methods A comparison was done on medical data of 30 children and 36 adults with blunt renal injury. The renal injuries were classified according to the organ inju-ry scaling committee of the American Association for the Surgery of Trauma. Results The rate of severe renal injury in the children group was 50%, significantly higher than 31% in the adult group(P<0.05).Surgical exploration rate in the children group was 40%, lower than 73% in the adult group(P<0.05).In the meantime, the kidney conservation rate of 93% in children group was higher than 64% in adult group(P<0.01). Conclusions The kidney of children is susceptible to severe injury but has sound serf-repairing capability. Therefore, surgical interventions and nephrectomy should be considered cautiously.
2.Researches and Thinking to Train Students' Innovative Spirit and Ability in Pharmaceutics Teaching
Hua HUANG ; Liangke ZHANG ; Jingqin ZHANG ; Wanyu LI
Chinese Journal of Medical Education Research 2005;0(05):-
This article manages to find some methods to improve students' innovative spirit and ability with some pharmaceutical teaching practice
3.Detection of hepatitis E virus RNA by real-time fluorescent RT-PCR
Yan YAN ; Chenyan ZHAO ; Zhuo LI ; Jingqin NIU ; Baoshan YAN ; Wa HAO ; Jiming YIN ; Youchun WANG
Chinese Journal of Laboratory Medicine 2009;32(2):175-178
Objective To investigate the clinical significance of detection of hepatitis E virus (HEV) RNA in sera from patients with acute hepatitis E using real-time reverse transcription (RT)-PCR to detect hepatitis E virus RNA in sera from patients with acute hepatitis E.Methods A real-time RT-PCR assay, which can amplifies and detect the conserved region on ORF3, was used in this study. 434 outpatients and hospitalized patients with acute HEV infection was enrolled into this study.Simultaneously,the serum samples from 40 patients with HAV infection, 100 patients with HBV infection and 110 healthy blood donors were collected as the control The real-time RT-PCR was performed to detect HEV RNA in all these sera.Results 232 sera (53.5%) were positive for HEV RNA by real-time RT-PCR and all of the control were negative.The results of real-time RT-PCR and anti-HEV IgM (ELISA) were concordant in 67.1% samples.There was significant difference between the two methods ( Kappa = 0.308, P = 0.000 ).The first serum sample from five serum samples of the patients was positive for HEV RNA and negative for anti-HEV IgM.Follow-up studies showed all the five sera samples were positive for anti-HEV IgM.HEV RNA in serum could be detected between 2 and 10 days.Conclusions The real-time fluorescent RT-PCR method has high specificity, and can be applied to the qualitative detection of the serum with genotypes Ⅰ and Ⅳ of hepatitis E virus.Its clinical use can improve the early diagnosis of HEV.
4.Observation on the effect of self-made Chinese herbal medicine in preventing the occurrence of aspiration pneumonia in elderly patients with nasal feeding
Qinya ZHU ; Xiaoying YU ; Qinye JIANG ; Yanhong LU ; Jianlan LI ; Jingqin HU
Chinese Journal of Biochemical Pharmaceutics 2017;37(9):96-97
Objective To observe the clinical effect of Chinese herbal medicine gargle on the prevention of aspiration pneumonia in elderly patients with long-term nasal feeding. Methods 93 elderly patients over 60 years old with indwelling nasogastric tube for 30 days were randomly divided into 48 cases of the experimental group and 45 cases of the control group. We compared incidence of aspiration pneumonia of the elderly patients with long-term nasal feeding in two groups of oral care solution by using the self-made traditional Chinese medicine gargle for oral nursing in the experimental group and treated with saline in the control group. Results The oral bacterial colony count and the incidence of aspiration pneumonia in the experimental group were lower than those in the control group, with two different kinds of nursing fluid. Conclusion Using the self-made traditional Chinese medicine gargle for oral care, could effectively reduce the number of oral bacteria, reduce the incidence of aspiration pneumonia, the application effect is good, and could be in clinical promotion and application.
5.Study on genotype and subgenotype distribution of hepatitis E virus among patients with acute sporadic hepatitis E in Beijing
Jiming YIN ; Chenyan ZHAO ; Zhuo LI ; Yan YAN ; Jinpin FAN ; Wa HAO ; Hongxia MA ; Jingqin NIU ; Youchun WANG
Chinese Journal of Laboratory Medicine 2009;32(9):989-992
s E in Beijing belong to HEV genotype Ⅳ.
6.RBP2 induces stem-like cancer cells by promoting EMT and is a prognostic marker for renal cell carcinoma.
Dahai ZHOU ; Vinodh KANNAPPAN ; Xiang CHEN ; Jingqin LI ; Xuefeng LENG ; Jinping ZHANG ; Shiying XUAN
Experimental & Molecular Medicine 2016;48(6):e238-
Renal cell carcinoma (RCC), one of the most common kidney cancers, has a poor prognosis. Epithelial to mesenchymal transition (EMT) is a hallmark of carcinoma invasion and metastasis. Several studies have examined the molecular regulation of EMT, but the relationship between histone demethylases and EMT is little understood. In this study, we investigated the role of retinoblastoma-binding protein-2 (RBP2), a histone demethylase that is highly expressed in RCC and is positively correlated with poor RCC prognosis in the regulation of EMT. We found that ectopic overexpression of RBP2 can induce cancer stem cell-like (CSC) phenotypes through EMT in RCC cells by converting them to a more mesenchymal phenotype. This results in increased resistance to apoptosis, which leads to enhanced tumor growth in xenograft models. Together, our data show that RBP2 is an epigenetic regulator that has an important role in the initiation of CSC phenotypes through EMT, leading to tumor progression. RBP2 is also a novel biomolecule for RCC diagnosis, and prognosis and may be a therapeutic target.
Apoptosis
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Carcinoma, Renal Cell*
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Diagnosis
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Epigenomics
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Heterografts
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Histone Demethylases
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Histones
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Kidney Neoplasms
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Neoplasm Metastasis
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Phenotype
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Prognosis
7.Brachytherapy with 125I seed strand for the treatment of implanted main portal vein tumor thrombus:an experimental study in rabbits
Yun TAO ; Wenhui LI ; Qingxin LIU ; Jianjun LUO ; Wen ZHANG ; Jingqin MA ; Minjie YANG ; Xudong QU ; Zhiping YAN ; Lingxiao LIU ; Jianhua WANG
Journal of Interventional Radiology 2017;26(8):727-731
Objective To evaluate the safety and efficacy of brachytherapy with 125I seed strand in treating implanted main portal vein tumor thrombus (MPVTT) in experimental rabbits.Methods VX2 tumor cell line was implanted in the main portal vein (MPV) of 32 New Zealand white rabbits to establish MPVTT models.The rabbits were randomly divided into the treatment group (group T,n=16) and the control group (group C,n=16).125I seed strand was implanted in the MPVTT of the rabbits of group T,while blank seed strand was implanted in the MPVTT of the rabbits of group C.After the implantation,the changes in general condition,body weight and laboratory testing results were recorded.Two weeks after the treatment,every 8 rabbits from each group were sacrificed,and the specimens were collected and sent for pathological examination.The remaining rabbits were fed till they died,and then autopsy was conducted.Multi-slice spiral CT manifestations,histopathological findings,Ki-67 labeling index and apoptosis index were used to assess the curative effect,and the results were compared between the two groups.Results At each observation time point after brachytherapy,the weight loss of the experimental rabbits was more obvious in group C than in group T.No statistically significant differences in liver functions and white blood cell count existed between the two groups (P>0.05).The mean MPVTT volume of group T and group C were (565.40±220.90) mm3 and (2 269.90±437.00) mm3 respectively (P<0.001);the Ki-67 labeling indexes were (4.14±1.84)% and (33.82± 6.07)% respectively (P=0.001);the median survival days were (39.50±2.37) d and (27.38±1.22) d respectively (P=0.001).Conclusion For the treatment of implanted MPVTT in experimental rabbits,brachytherapy with 125I seed strand is safe and effective.