Objective To compare the contents of guanosine and adenine in peels of three strains of Trichosanthes kirilowii Maxim.;To provide references for germplasm resources evaluation and breeding of the Chinese medicinal materials. Methods HPLC method was used to determine the contents of guanosine and adenine with Waters AtlantisT3-C18 column (4.6 mm×150.0 mm, 5 μm). The mobile phase was methanol and water, and the detection wavelength was 254 nm. Results The contents of guanosinein in peels of three strains of Trichosanthes kirilowii Maxim. were 0.159 4, 0.159 6, 0.134 1 mg/g, respectively;the contents of adenine were 0.097 1, 0.127 9, 0.093 4 mg/g, respectively. Conclusion There were significient differences in the contents of adenine among three strains of Trichosanthes kirilowii Maxim.. Strain Ⅱ is with higher level of guanosine and adenine, which implies that the breeding of the Chinese medicinal materals is one way to improve the quality of Trichosanthis Exocarpium.

As one important part of external conditions,a harmonious social environment is necessary precondition for human health.This article inquires the function of a harmonious social environment to human health from internal demand,basic prerequisite and post-disaster social environmental support of human health.

Endostatin is an antitumor molecular targeting angiogenesis of tumor and plays an inhibitory role in tumorigenesis through inhibiting pathological angiogenesis, proliferation and metastasis of tumor. As increasing drugs for targeting therapy aim in clinic, endostatin has become one of hot spots of research on combined treatment of cancer in recent years.

Anti-Lipid-Peroxidation of Hovenia dulcis Thunb. in mice was studied. Results showed that H. dulcis Thunb. had the following functions: 1. decrease MAD contents of serum and tissucs of liver and brain. 2. increase SOD activity in tissues of liver, kidney and brain. 3.impart tolerance toward cold and heat, and prolong swimming time of mice. The results indicate tLat H. dulcis Thunb. has function of Anti-Lipid-Peroxidation and may be valuable for the retardation of senility.

Hypoxia inducible factor-1α (HIF-1α)-vascular endothelial growth factor (VEGF)-vascular endothelial growth factor receptor-2 (VEGFR-2) signaling pathway plays a vital role in the regulation of neovascularization,and the activation of this pathway is closely related with tumor invasion and metastasis.

Radiotherapy is the principle method of non-surgical treatment for esophageal cancer.The 5-year survival rate ranges from 20％ to 40％.In recent years,the studies have found that anti-angiogenesis therapy can not only directly inhibit tumor nutritional intake,but also turn around tumor hypoxia by normalizing tumor vessels so as to enhance the radio-sensitivity of tumor ceils,which is expected to become an effective way to improve the curative effect of esophageal cancer radiotherapy.

According to teaching outline and clinical training characteristic of psychophy-laxis speciality(including Psychiatry and Medicopsychology),we explored how to strengthen the clinical thinking ability and clinical skill training for clinical medicine students based on cultiva-tion of elementary knowledge and theory,and we also established a set of more appropriate item system of clinical skills assessment so as to improve the practice quality in psychiatry and medical psychology departments.

Objective To investigate the radiosensitizing effect of tetrandrine in human esophageal carcinoma cells (TE1) in vitro and its related mechanisms. Methods The cell proliferation was assessed by MTT assay. Colony formation was used to analyze radiosensitivity enhancement by tetrandrine in TE1cells. Western blotting was preformed to measure the cyclin B1 protein levels. Results Tetrandrine inhibited cell growth in a concentration and time depedant manner. The inhibition of proliferation was observed when cells were treated by 1.0, 5.0 and 10. 0 μg/ml tetrandrine for 24 h after irradiation ( P ＜0. 05;F= 3.09, 10.43 and 24. 00, respectively). The inhibition was more significant when cell were treated by 0. 1, 1.0, 5.0 and 10. 0 μg/ml tetrandrine for 48 h than 24 hours after irradiation (F =4. 12,12. 77, 44. 28, and 48.53 respectively ,all P ＜ 0. 01 ). The D0, Dq and SF2 decreased with the increase of the tetrandrine concentration. The maximal sensitizing enhancement ratio was 1.60 with 0. 5 μg/ml tetrandrine. Tetrandrine upregulated the expression of cyclin B1 and removed G2 / M arrest . Conclusions Tetrandrine can enhance radiosensitivity of TE1 cells. This effect may be associated with the increase of cyclin B1 expression to remove G2/M arrest.

Objective To discuss the value of Valsalva experiment combined with improved MRI tilt tomography applying on the pharyngeal ostium of eustachian tube opening function of patients with nasopharyngeal carcinoma.Methods 1 7 cases with patholog-ically proved NPC underwent conventional MRI plain and enhanced scan.In the eustachian tube area oblique transection T2 WI and T1 WI scanning was done before and after the Valsalva experiment.The invasion of eustachian tube and its surrounding structures was analyzed and the eustachian tube function was evaluated.Results Valsalva experiments combined with improved MRI trans-verse oblique scan could clearly display the whole structure of the eustachian tube and surrounding tissue invasion compared with conventional scanning MR scan.And it could better evaluate the eustachian tube opening function for patients suffered nasopharynge-al carcinoma.Conclusion Valsalva experiments with improved MRI oblique transverse imaging could achieve individualized scan of the eustachian tube,and could be used for evaluating the pharyngeal opening function of eustachian tube in patients with nasopharyn-geal carcinoma.

Objective To investigate the influence of atorvastatin on tumor necrosis factor - α (TNF- α) of sepsis rats.Methods Sepsis models were established using male SD rats by cecal ligation and puncture (CLP).A total of 100 healthy rats were divided into 4 groups ( n =25) randomly ( random number):sham- operation group,CLP group,low- dose atorvastatin group and high -dose Atorvastatin group.Blood samples of 5 rats in each group were collected at postoperative 0,3,6,12 and 24 hours,to detect tumor necrosis factor - α (TNF - α) levels in plasma.Observe and compare the mobility of rats in each group and specimens of small intestine were taken for histopathological examination by optical microscope.Results At 0 hour,plasmic TNF - α levels in 4 groups were statistically equal (P ＞ 0.05 ).In plasma of sham - operation group,changes of TNF - α levels were not obvious.Compared with CLP group,TNF - α levels in low - dose and high - dose Atorvastatin groups were both significantly lower ( P ＜0.01 ) at postoperative 3,6,12 and 24 hours.And TNF-α levels in high -dose Atorvastatin group were significantly lower than those in low - dose group ( P ＜ 0.01 ) at the 4 time points.The mortality of sepsis was higher in CLP model group than other groups,That of Atorvastatin group was significantly lower than CLP model group but higher than control group and high - dose group was lower than low - dose group.Conclusions Atorvastatin can inhibit the expression of TNF - α in blood plasma of sepsis rats and reduce inflammatory reaction.