The effects of DMSO and nifedipine on experimental myocardial infarction induced by isoproterenol(ISP)were investigated.The results showed a significant reduction in myocardial FFA and MDA contents in DMSO and nifedipine-treated groups, as compared with the ischemic group, and a beneficial effect on activity of SOD,GSH-px and Ca~(2+)-Mg~(2+)-ATPase. The effects of OH. and Ca~(2+) in myocardialg infarction were further analysed in this study.

Objective: To observe the clinical efficacy of warm needling combined with iontophoresis of Chinese medicine for temporomandibular joint disorder (TMD). ＜br> Methods: A total of 100 TMD cases were randomly allocated into a treatment group and a control group, 50 in each group. Cases in the treatment group were treated with warm needling combined with iontophoresis of Chinese medicine, whereas cases in the control group were treated with Western medication. The therapeutic efficacies in two groups were assessed after three courses of treatment. ＜br> Results: The recovery rate and total effective rate were 54.0% and 98.0% respectively in the treatment group, versus 30.0% and 82.0% in the control group, showing significant differences (bothP＜0.05). The recovery rates in the treatment group were higher than those in the control group respectively after 1, 2 and 3 courses of treatment, indicating that warm needling combined iontophoresis of Chinese medicine could substantially shorten the course of treatment. ＜br> Conclusion: Warm needling combined with iontophoresis of Chinese medicine is remarkably effective for TMD and deserves further clinical application.

AIM: To observe the pathologic changes in lung and the role of p38 MAPKinase signal pathways in pulmonary alteration in diabetic rats. METHODS: Diabetic rats were induced by intraperitoneally injected streptozotozin (STZ). After 4 weeks,we observed the pathologic changes in lungs,tested protein kinase C (PKC) activities by isotope in lungs of model rats,tested transforming growth factor (TGF-?_1) by Western blotting and immunohistochemical analysis,and determined the expression of p38 MAPKinase mRNA using in situ hybridization.RESULTS: After STZ administration for 4 weeks,we observed thickened pulmonary capillary basal lamina and increased number of fibre in Diabetes mellitus (DM) rats. TGF-?_1 levels,PKC and p38 MAPK activities were also found increased. CONCLUSION: The increased activities of TGF-?_1 and p38 MAPK suggeste that TGF-?_1 may play an important role in diabetic lung,and hyperglycemia-PKC-p38 MAPK signal pathways may be involved in the pathogenesis of diabetes.

Objective To explore the protection effects of the reduced form of nicotinamide-adenine denucleotid(NADH) on cardiac fibroblasts apoptosis induced by hydrazine in vitro.Methods Cardiac fibroblasts isolated from neonate rats were divided into four groups.In NADH pretreatment before hydrazine treatment group,the cardiac fibroblasts were cultured first in the medium containing 400?g/ml NADH for 2 hours,and then cultured in the medium containing 400?g/ml NADH plus 2mmol/L hydrazine for 72 hours;in hydrazine treatment group,the cardiac fibroblasts were cultured first in normal medium containing 400?g/ml NADH for 2 hours and then cultured in the medium containing 2mmol/L hydrazine for 72 hours;in control group,the cardiac fibroblasts were cultured only in normal medium for 72 hours;and in NADH pretreatment control group,the cardiac fibroblasts were cultured in medium containing 400?g/ml NADH for 72 hours.After the treatments mentioned above,the changes in nuclear of apoptotic cardiac fibroblasts as stained by Hoechst33258 were observed under fluorescence microscope,the apoptotic and necrosis rates of cardiac fibroblasts stained by AnnexinⅤ/PI were assayed by flow cytometry,the mitochondria membrane potential of cells stained by rhodamine123 was also assayed by flow cytometry.Results Hydrazine could induce apoptosis of the cardiac fibroblasts.After NADH pretreatment,the number of apoptotic cardiac fibroblasts with pyknosis of nuclear was lowered,the rates of apoptosis and necrosis decreased,and the mitochondria membrane potential of cells was elevated.Conclusion NADH pretreatment could reduce the apoptosis induced by hydrazine by improving the function of mitochondria.

BACKGROUND: In the tissue engineering studies, the common method of cell detachment is trypsinization. However, this method may decrease cell activity by breaking the extracellular matrix proteins. Therefore, it is important to develop a new cell detachment method which can avoid the damage from trypsinization.OBJECTIVE: Based on that poly N-isopropylacrylamide (PNIPAAm) could alter its hydrophobic property with the change of temperature, this study aimed to develop a novel method which can control cell detachment by simply changing the temperature. METHODS: IPAAm was dissolved in isopropanol with final concentration of 55% (w/v). IPAAm solution was evenly laid on the surface of TCPS dish, and PNiPAAm was then covalently grafted on the TCPS dish surface by electron beam irradiation. The micro.structure of the surface of TCPS dish was observed by atomic force microscopy (AFM) before and after PNIPAAm grafted. Water contact angle was determined by sessile drop method. And an inverted phase contrast microscope was used to observe and evaluate the cell detachment ability of PNIPAAm grafted TCPS dish by subjecting the same dish to different temperatures. RESULTS AND CONCLUSION: AFM images showed that a thin and compact PNIPAAm layer formed on the TCPS dish surface. In the water contact angle test, there was no statistic difference between PNIPAAm grafted and ungrafted surfaces at 37 ℃, and the water contact angle of PNIPAAm grafted surfaces was lower than that of ungrafted surface at 20 ℃. The cells adhered to the surface of the PNIPAArn grafted TCPS dish at 37 ℃, which would be detached at 20 ℃.It was successful to control cell detachment by simply changing the temperature with PNIPAAm grafted TCPS dish, and it would be promising to be applied in tissue engineering.

ObjectiveTo observe the clinical efficacy of electroacupuncture plus herbal-partitioned moxibustion in treating temporomandibular joint disorder.MethodNinety patientswith temporomandibular joint disorder were randomized into a treatment group of 45 cases and a control group of 45 cases. The treatment group was intervened by electroacupuncture plus herbal-partitioned moxibustion, while the control group was by medication. The therapeutic efficacy was evaluated after 3 treatment courses by a specially-assigned person.ResultThe recovery rate and total effective rate were respectively 64.4% and 97.8% in the treatment group versus 37.8% and 82.2% in the control group, andthe total effective rate and recovery rate in the treatment group were significantly higher than that in the control group (P<0.05); regarding the comparison of the efficacy and treatment duration of the recovered subjects, the recovery rates at the end of the 1st course, 2nd course and 3rd course in the treatment group were all markedly higher than that in the control group (P<0.05), indicating that electroacupuncture plus herbal-partitioned moxibustion can shorten the period of treatment for temporomandibular joint disorder. The therapeutic efficacy of the treatment group was significantly superior to that of the control group(P<0.05).ConclusionElectroacupuncture plus herbal-partitioned moxibustion can produce a significant efficacy in treating temporomandibular joint disorder.

0.99).The limits of detection were below 0.5 ?g/L.The rates of recovery for the spiked samples ranged from 70.1% to 117.9% and the relative standard deviations(RSD) were between 0.4%-16.1%.Conclusion The method is simple,rapid,accurate and is applicable to the simultaneous determination of multi-pesticide residues in drinking water.

Objective To investigate the effects of Topiramate(TPM) on activity of SOD and concentration of MDA in blood serum and the changes of the nerve function scores after cerebral ischemia/perfusion in rats. Methods Male Sprague-Dawley rats were randomly divided into ischemia/perfusion group, TPM group and sham-operation group. Rat models of transient focal cerebral ischemia were made by 2 h occlusion of right middle cerebral artery occlusion and reperfusion for 24 h.Rats in TPM group were injected TPM(8 mg/ml,80 mg/kg)in the beginning of the artery occlusion and the reperfusion. The rats were sacrificed after they were evaluated by the nerve function deficiency scores. The activity of SOD and concentration of MDA in blood serum were measured.Results The activity of SOD and concentration of MDA in blood serum in ischemia/perfusion group were (157.72?19.04)U/ml and (7.45?0.84 )nmol/ml, those in TPM group were ( 171.25?15.72)U/ml and (( 6.10?0.98) nmol/ml,those in sham-operation group were (179.74?7.95)U/ml and (5.90?0.72 )nmol/ml. Compaired with sham-operation and TPM groups, the activity of SOD in ischemia/perfusion group was significantly lower, the concentration of MDA was obviously higher (all P

Objective To investigate the neuroprotective effects of Topiramate (TPM) on rat brain with ischemia-reperfusion damage and its mechanisms.Methods The 30 male SD rats were randomly assigned to sham operated group, ischemia-reperfusion group and TPM treated group.The cerebral ischamia and reperfusion model was made by suture occlusion of right middle cerebral artery(MCAO).Rats in TPM treated group were intraperitoneally injected TPM (80 mg/kg) twice. At 24 h following onset of MCAO,the nerve function score was evaluated with Neurological Grading Scale. The infarction volume was measured with TTC staining. The contents of glutamate (Glu) and gamma-aminobutyric acid (GABA) of cerebral cortex were tested by the high performance liquid chromatography with fluorescent detection. GABAA receptors were observed by immunohistochemistry.Results (1) Compared with the ischemia-reperfusion group, the Neurological Grading Scale of TPM treated group was significantly higher(P