Objective To compare the difference of clinical effect on the thoracic trauma patients between conventional thoracotomy(CTH) and video-assisted thoracoscopic surgery (VATS), and the possible mechanism.Methods Two hundred thoracic trauma patients were enrolled from 2007 January to 2010 March,and divided into VATS group( n =98) and CTH group( n = 102) according to the operative methods. The levels of serum high sensitive C-reactive protein(hs-CRP) ,interleukin-6 (IL--6) and tumor necrosis factor-α (TNF-α) were determined before and after operation. Intraoperative blood lost, operative time, postoperative analgesic time and hospitalization time were recorded and analyzed. Results There were no differences on the levels of inflammatory factors between the two groups before operation(P＞0.05) ,but the levels of inflammatory factors of VATS group were significantly decreased than that of CTH group after operation ( all P < 0. 05). Intraoperative blood lost, operative time, postoperative analgesic time and hospitalization time of VATS group were also lower than that of CTH group ( all P < 0. 05 ). Conclusion Compared with conventional thoracotomy, video-assisted thoracoscopic surgery had the characteristics of less intraoperative blood lost, shorter operative and postoperative analgesic time, and rapid recovery, could be relative to less inflammatory reaction.

Objective:To optimize the extraction process of total flavonoids in Gynostemma pentaphyllum by the method of response surface analysis ( RSM) . Methods: Based on the choice of factors and levels according to the results of single factor test, Box-Be-hnken response surface experiment was designed with the extraction rate of total flavonoids as the index to obtaln the best operation con-ditions and two equations of the response surface model. Results: The optimal extraction conditions of total flavonoids in Gynostemma pentaphyllum were as follows:the volume fraction of ethanol was 71%, the ratio of solid to liquid was 1∶14, and the ultrasonic time was 32 min. Under the conditions, the model predictive value of extraction rate of total flavonoids in Gynostemma pentaphyllum was 4. 676%, and the extraction rate in the verification test was 4. 641%. Conclusion:The fit of the regression model is good, and the ex-traction technology is feasible and reliable.

This study aims to explore the effects of alginate-poly ornithine-alginate (A-PLO-A) and barium alginate-poly ornithine-alginate (B-PLO-A) microcapsules as cells carriers during implantation. Mice hepatocytes coated in A-PLO-A and B-PLO-A microcapsules were implanted into rats with acute liver failure caused by intraperitoneal injection of D-galactosamine. The rat survival rate, liver cell growth, proliferation and metabolism within the microcapsules were investigated, as well as its effect on the improvement of rat acute liver failure. The influence of A-PLO-A-free microcapsules, B-PLO-A-free microcapsules, isolated liver cells, A-PLO-A microcapsule-coated and B-PLO-A microcapsule-coated liver cells was studied. It was found that the chemical-free microcapsules showed no positive effect on the rats with liver failures, with a death rate of 100% in both groups 3 days after the implantation. The ALT, AST and ALB levels were all improved in the isolated liver cell group, the A-PLO-A microcapsule-coated and the B-PLO-A microcapsule-coated groups. The survival rate of both microcapsule-coated liver cell groups was significantly higher than that of the chemical-free microcapsule group and the isolated liver cells group. The microcapsules were retrieved after 4 weeks' implantation, which were observed to be smooth with no cells attaching to the surface. No apparent fibrosis was observed. This research demonstrated the physical stability and the biocompatibility of the PLO-based alginate microcapsules and therefore they could be used as liver cell carriers during implantation.
Alginates ; administration & dosage ; Animals ; Glucuronic Acid ; administration & dosage ; Hepatocytes ; transplantation ; Hexuronic Acids ; administration & dosage ; Liver Failure ; therapy ; Mice ; Ornithine ; administration & dosage ; Rats

The classification messages of non-powered suction apparatus device (NPSAD) intended for negative pressure wound therapy by CFDA have been analysis and generalized. A set of classification regular patterns of NPSAD have been generalized from its intended use, composition, mechanism, contact area and resorbable characteristic. It is helpful to draw a more consistent classification in NPSAD.
Humans ; Negative-Pressure Wound Therapy ; classification ; Suction ; instrumentation

Objective: To observe the effect of distraction osteogenesis and synostosis following the placement of implant-distraction apparatus. Methods: The bilateral premolars were extracted in six adult hybrid dogs. After twelve weeks, a box-shaped osteotomy on the random side of the alveolar bone was carried out and two implant-distraction apparatus were placed. After one week, the alveolar ridge was vertically augmented at 1.05 mm/d by distraction for four days. Following 6-week-fixation histological and radiographical evaluations were carried out. Results: All dental implant-distraction apparatus obtained good healing except one failed for inflammation and was removed. The alveolar ridge was vertically augmented 4. 00 mm on average. The radiolucency in the distraction site disappeared in 6 weeks. Histologically, the lengthened bone consisted of mature lamellar and canellous bone, and good synostosis was obtained. The transport bone segment was vertical- ly augmented 4.2 mm, the maximum augmentation was 5 mm. Conclusions: The dental implant-distraction apparatus may be used for vertical augmentation of the alveolar ridge.

The vast majority of vertebral compression fractures are caused by osteoporosis and vertebral tumor,which lead to the pain of a fracture and intervertebral height lost.In recent 20 years,the use of percutaneous vertebroplasty and percutaneous kyphoplasty,a kind of minimally invasive surgery method intended for the vertebral compression fractures,are rising up for patients with vertebral compression fractures,which can quickly relieve patients' pain and restore injured vertebral deformities.Percutaneous vertebroplasty injects bone cement into fractures through a small incision.However,percutaneous kyphoplasty injects a balloon into a fracture to make a cavity by expending and shrinking,which is filled with filler material,and then removes the balloon,and injects bone cement.This article mainly reviews development,curative effect and safety,clinical application and future aspects.of the percutaneous vertebroplasty and percutaneous kyphoplasty.

Objective To evaluate the factors affecting the treatment of old persons diagnosed hypertension. Method A sample of 55566 persons from the community of Yanji area of Shanghai was recruited. Results Among the 19295 persons over sixty there are 5842 patients with hypertension, the morbidity was 30.3%. Among the 36271 persons aged fifteen to fifty-nine there are 3536 patients with hypertension, the morbidity is 9.7%. The ratio of non-treatment in the old age group is 3.1% and in younger group is 8.8%. However,the compliance to the treatment was better in the younger than in old group(73.7% vs 56.2%). The age,hypertension grade and sex were all accounted for the differences in the regularity of the hypertension treatment. Conclusion Further effort on the education of non-old hypertensive patients is essential to decrease the damage from hypertension.

Objective To measure the mandibular thickness relative to the osteotomy line of mandibular angle plasty so that to provide the anatomical basis for operation.Methods 37 youth women patients mandible were scanned by spiral CT,then the three-dimensional reconstruction was done,the thickness of the mandible around osteotomy line were measured on the planes corresponding to the posterior margin of mandibukar ramus,the middle part of the mandibular ramus,the posterior line of the third molar,the line between the second and third molar,the line between the second and first molar and the line between the first molar and the second premolar.The data were analyzed by Spss 11.5.Results The thichest bone around the osteotomy line was under the second and third molar,then the bone thickness became thinner forward and backward.The thinnest bony was on the middle part of the mandibular ramus.Conclusion The result of this study is of significant for guiding operation and reducing the complications.

Objective To investigate the changes of adhesion molecules and their effects on skeletal muscle ischemia/reperfusion injury. Methods 42 Wistar rats were divided into 3 groups: normal control group (Group Ⅰ, n=6), ischemia group (Group Ⅱ, n=6),ischemia/reperfusion injury group (Group Ⅲ, n=30). The level of malondialdehyde (MDA) in the plasma, myeloperoxidase (MPO) in the skeletal muscle, CD11b/CD18 on the leukocytes, intercelluar adhesion molecule-1 (ICAM-1) in the skeletal muscle and the histological changes were studied 1 h, 2 h, 4 h, 8 h, 12 h reperfusion after ischemia for 4 h. Results In group Ⅲ, the expression of CD11b/CD18, ICAM-1 and the injury of skeletal muscle increased with the lapse of reperfusion time. They reached the peak at 8～12 hours' reperfusion. The injury of skeletal muscle developed with the expression of adhesion molecules. Conclusion The expression of CD11b/CD18 and ICAM-1 are significantly associated with the skeletal muscle ischemia-reperfusion injury.

The aim of this study was to investigate the pulsatile-flow performance in vitro of a new heart valve prostheses implanted with minimally invasive techniques (HVPMIT). Three HVPMITs were tested valves and another three original biological heart valve prostheses acted as reference valves. The pulsatile-flow parameters (including mean pressure drop, regurgitant percentage of stroke volume, and effective orifice area) were tested in a pulse duplicator according to the methods listed in ISO5840-2005 and GB 12279-2008. The results demonstrated that the regurgitant percentage of stroke volume of tested valves was up to 13%. It was significantly higher than that of the reference valves. This result suggested that paravalvular leakage had occurred in the tested valves. It was found in the further analysis that because HVPMIT was not sewn into the heart tissue when the HVPMIT was implanted in vivo and there was not a sewing ring in the HVPMIT, when tested valves were fixed in the pulse duplicator, some gaps might exist between the stent of HVPMIT and the fix gasket, and the paravalvular leakage could therefore take place through these gaps. This study demonstrated that there are significant differences in the shape, structure, fixation in vivo and clinical operational methods between HVPMIT and original biological heart valve prostheses. It is necessary to establish new test methods which adapt for HVPMIT to evaluate its pulsatile-flow performance according to its own features.
Animals ; Cardiac Catheterization ; Heart Valve Prosthesis ; Heart Valve Prosthesis Implantation ; adverse effects ; instrumentation ; methods ; Minimally Invasive Surgical Procedures ; methods ; Prosthesis Design ; Pulsatile Flow