[Objective]To assess the accuracy and value of radiographic technique using computed tomography(CT) measurements in cadaveric specimens during anterior cervical corpectomy in order to define the safe lateral limit of dissection.[Method]The gutter of C4～6 corpectomy on 5 cadaveric samples was filled with radioopaque dye.An antero-posterior X-ray was used to measure the narrowest distance from dye column to the line of uncovertebral joints.A CT scan confirmed the distance from the borderline of the gutter to vertebral arteries.[Result]The distance between the X-ray dye column and the line of uncovertebral joints averaged 4.7 mm in the left and 4.2 mm in the right.While the distance from the borderline of the gutter to vertebral arteries with CT scan averaged 6.1 mm in the left and 5.9 mm in the right.The measured distance by radiographic technique underestimated the true distance.[Conclusion]Intraoperative radiographic technique can estimate the lateral distance between the gutter of corpectomy and vertebral arteries by measuring the distance from the dye column to the line of uncovertebral joints.This technique provides surgeons an additional margin of safety during anterior cervical corpectomy.

Primary myelofibrosis (PMF) is a chronic myeloproliferative disorder in human bonemarrow. Over 50% of patients with myelofibrosis have mutations in JAK2, MPL, or CALR. However,these mutations are rarely detected in children, suggesting a difference in the pathogenesis ofchildhood PMF. In this study, we investigated the response to drug treatment of a monozygotic twinpair with typical childhood PMF. The twin exhibited different clinical outcomes despite following the same treatment regimen. The transcriptomic profiles of patient samples after drug treatment (E2and Y2) were significantly different between the twin pair, which is consistent with the observationthat the drug treatment was effective only in the younger brother, despite the twin being geneticallyidentical. Bioinformatics analysis of the drug-responsive genes showed that the JAK-STAT pathwaywas activated in the cured younger brother, which is opposite to the pathway inhibitionobserved in adult PMF cases following treatment. Moreover, apoptosis and cell cycle processes wereboth significantly influenced by drug treatment in the sample of younger brother (Y2), implyingtheir potential association with the pathogenesis of childhood PMF. Gene mutations in JAK2,MPL, or CALR were not observed; however, mutations in genes including SRSF2 and SF3B1occurred in this twin pair with childhood PMF. Gene fusion events were extensively screened inthe twin pair samples and the occurrence of IGLV2-14-IGLL5 gene fusion was confirmed. The currentstudy reported at transcriptomic level the different responses of monozygotic twin brothers withchildhood PMF to the same androgen/prednisone treatment regimen providing new insights into thepotential pathogenesis of childhood PMF for further research and clinical applications.

Objective To prepare specific mouse monoclonal antibodies against Homo sapiens dynein,axonemal, heavy chain 2 (DNAH2). Methods Firstly, recombinant plasmid encoding His tagged immunogen, targeting N-terminal sequence of DNAH2 protein (1-300 aa), in E. coli was constructed. IPTG was used to induce the expression of His-immunogen, which was then purified and immunized in BALB/c mice. Hybridoma cells were obtained through the fusion between myeloma cells and splenocytes isolated from BALB/c mice. Finally, ELISA and Western blot assays were performed to screen the positive hybridoma. Results IPTG was used efficiently to induce the expression of DNAH2 immunogen in E. coli. DNAH2 protein bands were detected in screened positive hybridoma. Conclusion Mouse monoclonal anti-DNAH2 antibody is prepared successfully.