KAI1 is a recently identified metastasis suppressor gene located at human chromosome 11 p11.2. KAI1 protein is a member of the structurally distinct family of cell surface glycoprotein and belongs to transmembrane 4 protein superfamily. KAI1 expression is down-regulated in many metastatic tumor cells. Down-regulation of KAI1 expression also plays an important role in the genesis and development of gynecological tumor.

Objective: To build a relationship model of perceived academic self-efficacy, learning stress, and learning burnout of high school students. Methods: 674 high school students were surveyed with Learning Stress Scale of High School Students, The Perceived Academic Self -efficacy Scale, Scale of Learning Burnout of High School Students. Results: Learning stress had a direct effect on a low level of learning efficacy, perceived alienation to teacher and physical exhaustion (The direct effect value were -0.22,0.12,0.27). At the same time, perceived basic ability and perceived control partially mediated the impact of learning stress on the low level of learning efficacy, perceived alienation to teacher and physical exhaustion (the mediated effect value were -0.155 and-0.264, 0.066 and 0.299, 0.089 and 0.233). Perceived basic ability and perceived control fully mediated the relationship between learning stress and emotional exhaustion (the mediated effect value were 0.033 and 0.387). Conclusion: The students with a higher level of stress reported suffering more learning burnout than those with a lower level of stress, and vice versa.

Objective To evaluate the infection of high-risk human papillomavirus (HPV) and the expression of tumor metastasis suppressor protein KAI1 in cervical cancer.Methods The expression of KAH,HPV16/18E6 and HPV16E7 proteins were analyzed by immunohistochemistry SP assay in 117 cases of paraffin-embedded cervical tissue,including 20 normal cerival tissue as control,58 intraepithelial neoplasia (CIN) and 39 cervical cancer.Results In normal cervical tissue,CIN and cervical cancer,the positive rate of KAI1 protein were respectively 90.0 ％ (18/20),72.4 ％ (42/58),25.6 ％ (10/39).There was significant differience among three gruop (P ＜ 0.01).The positive rate of HPV16/18E6 and HPV16E7 proteins were respectively 0 (0/20),31.0 ％ (18/58),41.0 ％ (16/39); 0 (0/20),34.5 ％ (20/58),64.1％ (25/39).There were significant differience among three gruops (P ＜ 0.01).There was no correlation between the expression of KAI1 and the infection of HPV (P =0.429).Expression of KAI1 was correlated to grade of differentiation,clinical stage and lymph node metastasis (P ＜ 0.05),but was not correlated to age (P ＞ 0.05).HPV infection was not correlated to the age,clinical stage,cell differentiation and lymph node metastasis (P ＞ 0.05).Conclusion The expression of KAI1 protein is down-regulated in cervical cancer,which is not associated with the infection of HPV.

OBJECTIVE:To evaluate the clinical efficacy of Xuebijing injection in treating acute paraquat poisoning.METHODS:35 patients with acute severe parquat poisoning who were admitted into ICU of our hospital from Jan.2002 to Apr.2008 were enrolled in our study:15 cases were assigned to control group,20(treatment group)received Xuebijing 100 mL bid for 7～10 d in addition to the therapy as in control group.RESULTS:Liver enzyme and creatinine levels in both group were reduced,with the level in the treatment group significantly lower than in control group(P

Objective To study the effects of glimepiride and short-term intensive therapy with insulin on plasma glucose and beta-cell function in newly diagnosed type 2 diabetic patients.Methods 80 newly diagnosed type 2 diabetic patients were divided into two groups of 40 patients each and randomly treated with insulin or glimepiride plus metformin for 8 weeks.The FBG,2hPBG,HbA_1c,improvement of beta-cell function were measured before and after intensive therapy in each group.Results After the treatment,FBG,2hPBG,HbA_1c were significantly decreased (all P<0.001) in each group;FCP and 2hPCP were increased(P<0.05)in each group.Conclusion Glimepiride or short-term intensive therapy with insulin plus metformin could effectively improve glycemic control and beta-cell function in newly diagnosed type 2 diabetic patients.

Brain Computer Interface(BCI) is a direct information communication and control channel established between human and computer or other electronics devices and it is a wholly new communication system that does not depend on the brain's normal output pathways of peripheral nerves and muscles.The general constitutions and principles of BCI systems are introduced.In addition,research methods based on electroencephalograph are discussed and the existing problems and future trends of BCI are pointed out.

Objective To evaluate the therapeutic efficacy of YunKe and prednisone on moderate and severe thyroid associated ophthalmopathy(TAO).Methods 1 08 hyperthyroidism patients with moderately severe TAO were randomly divided into YunKe group,hormone group and the control group.All of the patients were given anti -thyroid medication(ATD)treatment.By thyroid hormone level upper limit every times higher than normal for a sulfhydryl imidazole 5mg/day to calculate daily dosage,based on thyroid hormone level adjusted a sulfhydryl imidazole dose every two weeks,make thyroid hormone levels in the normal range.YunKe set:intravenous push note YunKe per day A +B agent four groups (including cloud 20mg),each treatment medicine for five days in A row,interval of 25 days to the next period of treatment,medication six procedures.Hormone group:oral prednisone for 6 months,monthly dose in the order 1 mg·kg -1 ·d -1 ,0.75mg·kg -1 ·d -1 ,0.5mg·kg -1 ·d -1 ,0.25mg·kg -1 ·d -1 ,0.1 25mg·kg -1 ·d -1 , 0.062 5mg·kg -1 ·d -1 ,1 time a day,took at 8 o'clock in the morning.The control group treated ATD.Before and 6 months after treatment,all of the patients were checked the TNF -αand TRab,CT scan to measure degree of exophthalmus.Six months after treatment,respectively,on the basis of TAO classification (NOSPECS)and TNF -α, TRab level changes to determine the efficacy of treatment.Data entry Excel 2003 statistical software,used SPSS 1 9.0 statistical software to analyze data.Non parametric tests of three groups of data with Kruskal Wallis test.Not matching the two independent samples nonparametric test with the Mann -Whitney test,with P <0.05 for the difference was statistically significant.Results The markedly effective rate,effective rate and ineffective rate of YunKe group were 36.1 %,47.2% and 1 6.7% respectively,which of the hormone group were 20.0%,37.1 % and 42.9%,which of the control group were 1 3.9%,22.2% and 63.9%.The efficacy among the three groups had statistically significant differences (Kruskal -Wallis χ2 =1 0.736,P =0.005 ).The lighter exophthalmos,tge curative effect was better (Mann -Whitney Z =-4.599,P <0.001 ).The changes of TNF alpha,TRab levels among the three groups had significant differences(YunKe group,the TNF alpha,TRab levels markedly reduced 75.0% and 80.6%,respectively, which of the hormone group were 37.1 % and 34.3%,which of the control group were 1 9.4% and 27.8%,TNF χ2 =23.527,P <0.001 ;TRab χ2 =23.831 ,P <0.001 ).Conclusion The therapeutic efficacy of YunKeon TAO is better than prednisone,but the long -term effect deserves more investigation.

BACKGROUND: To prepare a novel biological material that can be implanted into the lesion of bone tuberculosis, keep sustained release of anti-tuberculosis drugs around bone tuberculosis tissues for a long time, and enhance the effect on bone repair has become a hot spot for clinical studies on bone tuberculosis.OBJECTIVE: To prepare the adipose-derived mesenchymal stem cells (ADMSCs)/sustained-release rifampin-loaded microsphere complex, and to preliminarily study its effects in a rabbit model of spinal tuberculosis.METHODS: Rifampin-chitosan-calcium alginate sustained-release microspheres were synthesized, and co-cultured with ADMSCs to prepare an anti-tuberculosis composite for bone tissue engineering. Forty New Zealand rabbits were used to make lumbar L6 tuberculosis models. Thereafter, rat models were randomly divided into four groups and given rifampin by gavage in rifampin group, ADMSCs by paravertebral injection+rifampin by gavage in stem cell group, rifampin by gavage+implantation of the anti-tuberculosis composite material in experimental group, and no treatment in control group.The duration time of rifampin administration was 8 weeks. The anti-tuberculosis effect of the composite material was evaluated by X-ray and CT scanning observation.RESULTS AND CONCLUSION: In the control group, obvious damage to lumbar vertebrae L5 and L6 was apparent; inflammatory granulation tissues formed; and the intervertebral space was narrowed. In addition, two rabbits in the control group showed obvious kyphotic deformity and five showed pasoas major swelling with low-density dark region in the psoas muscle. In the rifampin group, there were five rabbits with moderate damage of the lumbar vertebrae L5 and L6,and two rabbits with pasoas major swelling. In the stem cell group, there were two rabbits with moderate damage of the lumbar vertebrae L5 and L6, three rabbits with mild damage of the upper part of the lumbar vertebra L6, and three rabbits with pasoas major swelling. In the experimental group, only four rabbits suffered from mild damage of the upper part of the lumbar vertebra L6 but with no changes in the intervertebral space between the L5 and L6, and without pasoas major swelling. These results indicate that the composite material of ADMSCs combined with sustained-release rifampin-loaded microspheres can inhibit mycobacterial growth effectively, and reduce vertebral bone destruction,thereby giving some therapeutic actions for the animal models with spinal tuberculosis.

OBJECTIVE:To evaluate the association between UGT1A1 gene polymorphism and irinotecan-induced 3-4 degree neutropenia,and to provide evidenced-based reference for clinical treatment. METHODS:Retrieved from CJFD,Wanfang data-base,VIP,PubMed,EMBase,Science direct and Cochrane library,related studies about UGT1A1*28 and UGT1A1*6 gene polymorphism and irinotecan-induced 3-4 degree neutropenia were collected. After data extraction and quality evaluation of included studies,Meta-analysis was conducted by using Review Man 5.3 software. RESULTS:A total of 29 studies were included,involv-ing 2408 patients. UGT1A1*28 includ wild genotype TA 6/6(UGT1A1*1/*1)and mutations genotype TA 6/7(UGT1A1*1/*28)、TA 7/7(UGT1A1*28/*28),UGT1A1*6 includ wild genotype GG and mutations genotype GA、AA. Results of Meta-analysis showed:the incidence of 3-4 degree neutropenia in UGT1A1*28 and UGT1A1*6 mutations genotype were significantly higher than wild genotype,with statistical significance [UGT1A1*28:OR=1.92,95%CI(1.52,2.44),P<0.001;UGT1A1*6:OR=2.49, 95%CI(1.46,4.26),P<0.001]. Using medium-dose and high-dose of irinotecan,the incidence of 3-4 degree neutropenia in UGT1A1*28 and UGT1A1*6 mutations genotype were significantly higher than wild genotype,with statistical significance [UGT1A1*28:OR=2.06,95%CI(1.57,2.70),P<0.001);UGT1A1*6:OR=1.92,95%CI(1.35,2.74),P<0.001]. Using low-dose of irinotecan,there was no statistical significance in the incidence of 3-4 degree neutropenia between UGT1A1*28,UGT1A1*6 mutations genotype and wild genotype [UGT1A1*28:OR=1.20,95%CI(0.70,2.08),P=0.51;UGT1A1*6:OR=3.19,95%CI (0.85,11.89),P=0.08]. CONCLUSIONS:Using medium-dose and high-dose of irinotecan,UGT1A1*28 and UGT1A1*6 muta-tions will increase the risk of severe neutropenia in cancer patients. Using low-dose of irinotecan,there is no clear correlation be-tween gene polymorphism and the neutropenia.

OBJECTIVE：To study the effects of Gushikangfuwan on osteoporosis caused by retinoic acid in rats. METHODS:The osteoporosis was induced by retinoic acid(70mg.kg-1,ig)daily in rats for two weeks. The pharmacologic effects of Gushikangfuvan was observed. RESULTS: Compared with the retinoic acid group Gushikangfuwan (6g.kg-1)enhanced the relative bone volume,average bone trabecula and bone Lacuna length in rats.Gushikangfuwan (3g.kg-1,6g.kg-1) also enhanced the content of calcium and phosphorus of thigh bone. CONCLUSIONS：Gushikangfuwan may ameliorate the bone quality and enhance the bone density in rats.