Objective To test whether murine bone marrow-derived stem cells (BMSCs) can contribute to the regeneration of renal tubular epithelial cells after ischemia-reperfusion (I/R) injury. Methods The female mice with bilateral I/R injured kidney received transplantation of bone marrow mononuclear cells isolated from homologous male mice via the tail vein after sublethal irradiation. After bone marrow transplantation (BMT), Sry expressions in the bone marrow of the recipient mice were detected by PCR. Serial-section analysis of Y chromosome fluorescence in situ hybridization and immunohistochemistry with CK18 or RCA were performed to observe the differentiation of bone marrow-derived stem cells in the I/R injured kidney. Results At 15 d after BMT, PCR analysis confirmed the presence of Sry gene expression in the bone marrow of the recipient mice. AT 30 d after BMT, Y +/CK18 + and Y +/RCA + cells were detected in the renal tubules of the recipients. Conclusion The microenvironment of the injured kidney can induce BMSCs to differentiate into renal tubular epithelial cells which express CK18 or RCA and participate in the regeneration of the injured renal tubule. The results suggest the clinical potential of BMSCs in the cell replacement therapy for renal failure.

Objective To study the physiological properties of a potassium channel in the basolateral membrane (BLM) of proximal tubule. Methods Nephrons were dissected from rat kidney under a stereoscopic microscope. The standard configuration for single channel tight seal patch clamp technique was used to record channel currents from the BLM. Results A kind of potassium channel with low conductance and inward rectification was easily recorded in the BLM of the proximal tubule. The channel current occurred spontaneously in 81% seals made on the BLM. In the patches, with no spontaneous channel activity, the channel current occurred when the pressure increased. In the inside out configuration, run down was present in channel activity. The open dwelling time constants of the potassium channel were 0.524 ms and 5.087 ms, while the closed dwelling time constants were 1.029 ms and 16.500 ms. Conclusion A type of potassium channels with low conductance, stretch sensibility and chemistry sensibility, presenting kinetic properties as two opening and two closing states, are widely distributed in the basolateral membrane of proximal tubule.

Objective To explore the effect of protease-activated receptor 1(PAR-1) activation on the expression of CXCR4 mR-NA of mouse endothelial progenitor cells (EPCs) and proliferation ,migration of EPCs .Methods Mouse EPCs were activated by different concentration of SFLLRN (one agonist of PAR-1) ,or transfected by small interfering RNA of PAR-1 .The expressions of PAR-1 and CXCR4 mRNA of EPCs were detected by fluorescent quantitative real-time PCR .Proliferation ,migration of EPCs were detected by MTT ,Transwell chambers respectively .Results The expressions of PAR-1 and CXCR4 mRNA in SFLLRN group were higher than that in other groups(P< 0 .05) .The expression of CXCR4 mRNA was highly positive correlation with PAR-1 mRNA(r=0 .991) .The proliferation ,migration of mouse EPCs were induced by activation of PAR-1 .Conclusion Activation of PAR-1 promotes cell proliferation and migration of mouse EPCs ,this effect may be depended on CXCR4 .

Objective To evaluate the feasibility of plasma NT-pro brain natriuretic peptide guided therapy of β1-blocker to patients with moderate and severe heart failure.Methods A total of 1 95 patients with moderate and severe heart failure were ran-domized to the clinical group and the BNP group.The use of β1-blocker was guided by monitoring clinical representation and the changes of BNP values respectively.The duration of initiative use ofβ1-blocker,the recurrence of heart failure,the mortality due to heart failure and the mean dosage ofβ1-blocker were observed and analysed.Results Compared with the clinical group,the time of initiative use ofβ1-blocker was significantly shorter in NT-proBNP group[(5.89±1.76)d vs .(7.03±2.08)d,P <0.01].The mean dosage ofβ1-blocker is significantly higher in BNP group than that in clinical group[(47.65 ± 13.09 )mg/d vs .(35.08 ± 1 1.08) mg/d,P <0.01].The recurrence and mortality of heart failure were similar in two groups(P >0.05).Conclusion NT-proBNP-guided therapy ofβ1-blocker might contribute to early use and tolerance of higher dosage ofβ1-blocker in patients with NYHA Ⅲ-Ⅳ class heart function,with no extra adverse event.

Traumatic rib fractures are the most common injury in thoracic trauma. Previously，the patients with traumatic rib fractures were mostly treated non-surgically，of which 50%，especially those combined with flail chest presented chronic pain or chest wall deformities and over 30% had long-term disabilities，being unable to retain a full-time job. In the past two decades，thanks to the development of internal fixation material technology，the surgical treatment of rib fractures has achieved good outcomes. However，there are still some problems in clinical treatment，including inconsistency in surgical treatment and quality control in medical services. The current consensuses on the management of regional traumatic rib fractures published at home and abroad mainly focus on the guidance of the overall treatment decisions and plans，and relevant clinical guidelines abroad lacks progress in surgical treatment of rib fractures in recent years. Therefore，the Chinese Society of Traumatology affiliated to Chinese Medical Association and Chinese College of Trauma Surgeons affiliated to Chinese Medical Doctor Association，in conjunction with national multidisciplinary experts，formulate the Chinese Consensus for Surgical Treatment of Traumatic Rib Fractures（2021）following the principle of evidence-based medicine，scientific nature and practicality. This expert consensus puts forward some clear，applicable，and graded recommendations from aspects of preoperative imaging evaluation，surgical indications，timing of surgery，surgical methods，rib fracture sites for surgical fixation，internal fixation methods and material selections，treatment of combined injuries in rib fractures，in order to provide references for surgical treatment of traumatic rib fractures.