Objective To explore the effect of electroacupuncture plus rehabilitation on shoulder-hand syndrome (SHS). Method Eighty SHS patients were randomized into an experimental group and a control group, 40 cases in each group. The experimental group was intervened by electroacupuncture plus rehabilitation training and basic Western medicine treatment, while the control group was by rehabilitation training and Western medicine treatment, 3 sessions a week, 4 weeks in total. The pain intensity of the affected shoulder and upper-limb motor function were observed before and after the treatment. The therapeutic efficacy was also evaluated. Result The Visual Analogue Scale (VAS) score of the shoulder pain and Fugl-Meyer Assessment (FMA) score of the upper-limb motor function were significantly improved after the intervention in the two groups (P<0.01), and the improvements of VAS and FMA in the experimental group were more significant than that in the control group (P<0.01). Conclusion Based on the basic treatment of Western medicine, electroacupuncture plus rehabilitation training can markedly improve the pain intensity of the affected shoulder and upper-limb motor function in post-stroke shoulder-hand syndrome.

Objective To investigate the clinical features,etiology and related clinical factors in neonatal anemia.Methods Two hundreds and sixty four infants admitted to the Neonatal Intensive Care Unit between January 2009 to March 2012 were retrospectively analysed with different levels,different age,different gestational age.Results Among 264 patients,the occupancy of blood loss anemia was 54.5 ％.Mild and medium neonatal anemia were more than severe anemia (172 vs 92).Fetomaternal hemorrhage syndrome occurred commonly in severe anemia (16.3％,15/92).Early anemia was more than late anemia(182 vs 82).Hemorrhagic anemia in early anemia (64.3％,117/82)occurred mainly.Late anemia with infection mainly (67.1％,55/82).Fetomaternal hemorrhage syndrome were all early anemia and term to account for a higher proportion (94.4％,17/18);twin-to-twin transfusion syndrome occurred commonly among preterm term infants (96.7％,29/30).Conclusion Blood loss anemia was the main etiological factor resulting in neonatal anemia.The incidence of mild and medium neonatal anemia was quite high.Early anemia was more than late anemia.There might be some differences in the etiology between term and perterm infants.

AIM To observe effects of IBMX on cGMP and GFAP production in gerbil hippocampus after recirculation following ischemia. METHODS Bilateral occlusion of common carotid arteries and immunofluorescent stain methods in gerbil hippocampal tissue slice were used. RESULTS Recirculation following ischemia leads to a rise in hippocampus cGMP and GFAP concentration. cGMP and GFAP positive cells mainly distributed in rediatum layer and molecular layer in the CA 1 subfield, Most of cGMP positive cells are astrocytes under double immunofluorescent stain. Most of cGMP strong positive cells are also those of GFAP strong positive cells. IBMX increased cGMP and GFAP production. CONCLUSION cGMP is possible to play an important role in the activation or regulation of GFAP in the hippocampus.

Objective To analyze the clinical effects of continuous epidural anesthesia and psychological intervention in painless labor. Methods From June 2016 to December 2016110 cases of maternal treatment in Ningbo city town of dragon longsai hospital as the research object, randomly divided into control group and observation group, control group of women to provide continuous epidural anesthesia, the observation group in continuous epidural anesthesia combined with psychological intervention measures. Results The two groups of pregnant women at different time scales VAS score, duration and other indicators, the data into the SPSS software, given the corresponding analysis and draw conclusions. Results The two groups of pain VAS score had no significant difference, the observation group each time after analgesia VAS score decreased than the control group(P<0.05); the first stage of the observation group was shorter than the control group (P<0.05), there was no significant difference between the 2 groups of second, third labor time. Conclusion Conclusion Epidural anesthesia and psychological intervention combined with painless labor have good analgesic effect and shorten the first stage of labor. .

OBJECTIVE:To observe clinical efficacy and safety of Carboxymethylstarch sodium solution in auxiliary treatment of pediatric bronchial asthma. METHODS:Totally 102 children with bronchial asthma selected from our hospital during Mar. 2015-Mar. 2016 were divided into observation group and control group according to admission order,with 51 cases in each group. Control group was given aerosol inhalation of Budesonide suspension for inhalation 0.5-1 mg,tid+ aerosol inhalation of Ipratropi-um bromide solution for inhalation 1-2 mL+Ambroxol hydrochloride and clenbuterol hydrochloride oral solution 2.5-15 mL,po, bid. Observation group was additionally given Carboxymethylstarch sodium solution 7 mL(1-<4 years old),10 mL(4-12 years old),po,tid. Both groups received treatment for 3 months. Clinical efficacy,the time of cough and wheezing relief,the time of lung wheezing disappearance,hospitalization time and the levels of immune indexes (IgG,IgM,IgA) were observed in 2 groups, and the occurrence of ADR was recorded. RESULTS:Total response rate of observation group(90.2%)was significantly higher than that of control group(70.6%),and the time of cough and wheezing relief,the time of lung wheezing disappearance and hospitaliza-tion time of observation group were significantly shorter than those of control group,with statistical significance (P<0.05). There was no statistical significance in the levels of IgG,IgM and IgA between 2 groups before treatment(P>0.05). After treatment,above immune indexes of observation group were significantly higher than before treatment and control group,with statistical significance (P<0.05). No obvious ADR was found in 2 groups during treatment. CONCLUSIONS:Carboxymethylstarch sodium solution in aux-iliary treatment of pediatric bronchial asthma shows good therapeutic efficacy and improves immunity of children with good safety.

Objective To evaluate the therapeutic effects of autolngous bone marrow mesenchymal stem cells transplantation in the early phase of acute renal ischemia-reperfusion(I/R)injury in rabbits and their impact on tubular epithelial cell apoptosis.Method Bone marrow mesenchymal stem cells(MSCs)were isolated and cultured in vitro.Thirty rabbits were divided randomly into transplanted group,control group and sham-operated group,10 in each group.Rabbits of transplanted group were induced acute renal I/R injury by clamping bilateral renal arteries for 90 minutes,then transplanted MSCs labeled with BrdU immediately after the resumption of circulation in kidney.The rabbits of control group were induced acute renal I/R injury and then infused with saline instead.The sharn operated rabbits were transplanted autologous MSCs labeled with BrdU after sham operation.Fortyeight hours after transplantation,all rabbits were sacrificed.Renal functional and structural damage were evaluated.MSCs derived BrdU positive cells were determined by immunostalning.Tubular epithelial cell apoptosis was arialyzed by usign TUNEL.The SPSS version 11.3 Software was used for statistical analysis.ANOVA were used to ana lyze the data of renal function and tubular epithelial cell apoptesis.Data of renal structure darnage were analyzed by using the nonparametric Kruskal-Wallis test.Results Compared with control group,the renal functional and struc tural darnage were significantly ameliorated(24 h,BUN:F=7.483,Ser:F:15.091;48 h,BUN:F:17.741.Scr:F=61.865;P<0.05),tubular epithelial cell apoptods Wsa reduced significantly in transplanted group(F=135.495,P<0.01).MSCs derived BrdU positive cell were detected in the renal tissues of transplanted rabbits.Conelusiu Autologous bone marrow mesenchymal stern cells transplantation is curative for acute renal I/R injury in rabbits.Bone marrow MSCs might ameliorate renal I/R injury by reducing tubular epithelial cell apoptosis.

Objective To investigate the kinetic expression level of chemokines (MCP-1 and MIP-2) in vagina of a murine model of vulvovaginal candidiasis (VVC).Methods The estrogen-treated murine model of VVC was set up.Vaginal specimens were obtained in different duration after inoculation of C.albicans intravaginally.Semi-quantitative RT-PCR was applied to determine MCP-1 and MIP-2 mRNA levels in these tissues.Results Compared with the control mice treated with olive oil,persistent growth of C.albicans was found from the 2nd day to 21st day after inoculation in estrogen-treated mice.Significantly higher levels of MCP-1 mRNA were observed in vaginal tissues in infected estrogen-treated mice than those in other 2 groups,infected but non estrogen-treated mice and estrogen-treated but uninfected mice.The high level of MCP-1 mRNA maintained from the 4th day to 21st day in infected estrogen-treated mice.It was also found that levels of MIP-2 mRNA were significantly higher in the vagina in the 2nd day in 3 groups of mice than those in naive mice,however,no significant difference was shown among 3 groups throughout the study period.Conclusion High level of MCP-1,rather than MIP-2,may be associated with susceptibility to VVC.

Objective To investigate the correlation between inducible nitric oxide synthase (iNOS) gene polymorphism and ischemic stroke in Chinese Han population.Methods Patients with first-ever stroke and the age-and sex-matched healthy controls were enrolled in the study.Taqman probe fluorescence quantitative polymerase chain reaction technique was used to detect the genotype distribution of rs2779248 C/T and rs1137933 C/T polymorphisms.Results A total of 246 patients with ischemic stroke and 246 controls were enrolled.The distribution frequencies of rs2779248 CC,CT and TT genotypes in the patient group were 57.7％,36.6％,and 5.7％,respectively,and in the control group were 68.7％,28.0％,and 3.3％,respectively.The T allele frequency of the patient group was significantly higher than that of the control group (24.0％ vs.17.3％;P =0.015).Multivariate logistic regression analysis showed that after adjusting for the risk factors including age,sex,hypertension,and diabetes,the risk of ischemic stroke in the CT + TT genotype carriers was 1.64 times of the CC genotype carriers (odds ratio 1.64,95％ confidence interval 1.07-2.51;P=0.022).The distribution frequencies of rs1137933 CC,CT and TT in the patient group were 58.1％,37.8％,and 4.1％,respectively and in the control group were 68.3％,29.3％,and 2.4％,respectively.The T allele frequency of the patient group was significantly higher than that of the control group (23.0％ vs.17.1％;P =0.013).Multivariate logistic regression analysis showed that after adjusting for traditional risk factors,the risk of ischemic stroke of patients with the TT + CT genotype carriers was 1.60 times of the CC genotype carriers (odds ratio 1.60,95％ confidence interval 1.05-2.46;P=0.030).Conclusions The rs2779248 C/T and rs1137933 C/T polymorphisms in iNOS gene may be associated with the risk of onset of ischemic stroke in Chinese Han population.

Acupoints are surface reaction and treatment spots under the pathological conditions which have functions of diagnosis and treatment. For the study of acupoints, we can carry it out from the aspects of structure and function. The two aspects have a close relationship. Structure is the material basis of function, while function is the manifesta-tion of structure. Therefore, the study of acupoints is not only significant to reveal the essence of acupoint, but also has enormous value in the guidance of acu-moxibustion for clinical practice. Staring from the normality of structure and variability of function, we explored the essence of acupoints, and further revealed ordered regulation rule and its biological basis, so that the essence of acupuncture points will be illustrated with scientific evidences.

Objective To explore the effect of enteral nutrition applied to bowel preparation of diabetes patients before electronic colonoscopy. Methods A total of 172 diabetes patients were randomly allocated to the experimental group and the control group with 86 patients in each group. The experimental groups executed no dietary restriction within 3 days before the examination, and received Ensure 1h before colonoscopy. However,the control group was given low residue diet within 3 days before the examination, and received no Ensure before colonoscopy. The enteral cleanness and incidence of hypoglycemic reaction, abdominal pain and horror was compared between the two groups. Results 72 cases(83.72%) of the experimental group and 70 cases (81.40%) of the control group had better enteral cleanness, and there was no statistical difference in intestinal cleanness between the two groups. In the experimental group the incidence of hypoglycemic reaction, abdominal pain, horror was significantly lower than those in the control group. Conclusions Bowel preparation with unrestricted diet and ingested ensure before colonoscopy can not only ensure intestinal cleanness but also enhance endurance of colonoscopy.