OBJECTIVE:To study the best preparation technology of Gross Saponins tribullu terrestris droping pill. METHODS: The ratio of Gross Spongines Tribullu terrestris to matrix, dripping temperature, dripping distance and dripping speed were investigated by preliminary test and orthogonal test with sphericity, the variation coefficient of weight of pill,time limit of dissolution and appearance of the dropping pill were taken as scoring indexes. Meanwhile, the technical conditions were optimized by orthogonal test on the basis of preliminary test.RESULTS: The optimal technical conditions were as follows: the ratio of matrix to Gross Saponins Tribullu terrestris was 5∶1;the dripping temperature was 85 ℃;the dripping speed was 8 d?min-1 and the dripping distance was 12 cm.CONCLUSION:The technology is simple, feasible and the evaluation indexes were reliable and reasonable and it can meet the requirement for dripping pill specified in China Pharmacopeia (2005 Edition).

Chronic high-salt diet-associated renal injury is a key risk factor for the development of hypertension. However, the mechanism by which salt triggers kidney damage is poorly understood. Our study investigated how high salt (HS) intake triggers early renal injury by considering the ‘gut-kidney axis’. We fed mice 2% NaCl in drinking water continuously for 8 weeks to induce early renal injury. We found that the ‘quantitative’ and ‘qualitative’ levels of the intestinal microflora were significantly altered after chronic HS feeding, which indicated the occurrence of enteric dysbiosis. In addition, intestinal immunological gene expression was impaired in mice with HS intake. Gut permeability elevation and enteric bacterial translocation into the kidney were detected after chronic HS feeding. Gut bacteria depletion by non-absorbable antibiotic administration restored HS loading-induced gut leakiness, renal injury and systolic blood pressure elevation. The fecal microbiota from mice fed chronic HS could independently cause gut leakiness and renal injury. Our current work provides a novel insight into the mechanism of HS-induced renal injury by investigating the role of the intestine with enteric bacteria and gut permeability and clearly illustrates that chronic HS loading elicited renal injury and dysfunction that was dependent on the intestine.
Animals ; Bacteria ; Bacterial Translocation ; Blood Pressure ; Drinking Water ; Dysbiosis ; Enterobacteriaceae ; Gastrointestinal Microbiome ; Gene Expression ; Hypertension ; Intestines ; Kidney ; Mice* ; Microbiota ; Permeability ; Risk Factors