Bolar exception is a patent infringement exemption principle .The article explored the origin ( market failure ) of Bolar ex-ception from the view of government regulation theory , and introduced the production , development and application status of Bolar excep-tion in the world.At the same time, the Bolar exception clauses in our country were also introduced , and the relative regulations in time, place and application scope were discussed in order to provide reference for the perfection of Bolar exception in China .

OBJECTIVE:To establish the quality standards for Weianning granules.METHODS:Radix Astragali,Hedyotic Diffusa,Radix Sophorae in Weianning granules were identified with TLC;Astragaloside Ⅳ in the granules was determined by HPLC-ELSD.RESULTS:The TLC spots were clear with high resolution.The linear range of Astragaloside Ⅳ was 2.567～12.840 ?g(r=0.999 6),with average recovery rate at 99.63%(RSD=4.69%,n=6).CONCLUSION:The est-ablished standards are suitable for the quality control of Weianning granules.

Neoplasms of the small intestine have no specific features at early stage,and the prognosis is poor.The 5-year survival rate of all the patients is about 30％ and the middle survival time is 19 months.The retrospective analysis shows that adjuvant chemotherapy after surgical resection might improve the disease free survival while the overall survival is not improved obviously.The patients who are at advanced stage or have regional lymph node metastasis may obtain benefits from adjuvant chemotherapy.Palliative chemotherapy may improve progress free survival and overall survival of patients who are at advanced stage.The therapeutic effect of patients at advanced stage using FOLFOX regimen ( 5-fluorouracil,calcium folinate and oxaliplatin ) is better compared with other regimens.The molecular targeted therapy of small intestine cancer is still in the study process and advanced studies are also needed for chemotherapy.

Objective To discuss the influence of Yin yoga for Ⅰ, Ⅱ knee osteoarthritis patients'quality of life and anxiety. Methods Divided 98 patients of Joint department outpatient service with joint specializes into the experimental group and the control group by random number method, there were 49 cases in each group, two group patients all have routine drug therapy that according to the guidelines of diagnosis and treatment of knee osteoarthritis in 2015,and accept the routine health education of orthopaedic. On the basis of this, the experimental group accept Yin yoga exercise training by a professional yoga teacher and nurse, they also followed up the two group of patients for 1 year. And adopting health questionnaire (SF-36) to assess the patient's quality of life on the same day, which they see the doctor ,3 months, 6 months, 12 months, they also use Self-evaluation of the anxiety scale (SAS) to assess the patient's anxiety. Results SAS scores before and after the intervention of patients in the experimental group was (57.14 ± 5.92) and (37.92 ± 3.07) points respectively, the difference was statistically significant (t=15.64, P<0.05), while the difference in the control group was not significant, (57.03 ± 6.18) points vs (53.18 ± 3.16) points , P > 0.05. Physiological function, physiological function, body pain, general health, energy, social function, emotional function, mental health of experimental group patients after 12 months intervention was (79.23 ± 9.15), (56.79 ± 13.20), (78.32 ± 10.15), (44.50 ± 11.92), (64.85 ± 9.72), (83.90 ± 11.61), (62.13 ± 18.72), (67.33 ± 8.50) points respectively, while in the control group was (70.22 ± 10.13), (32.12 ± 11.07), (60.14 ± 9.56), (39.40 ± 10.27), (48.66 ± 7.52), (67.62 ± 12.13), (43.23±14.89), (57.00±8.13) points respectively, the difference between the two groups was significant(t=3.18-15.54, P<0.05 or<0.01). Conclusions Yin yoga can improveⅠ,Ⅱknee osteoarthritis patients quality of life and all ease anxiety. The method is feasible.

Objective To investigate the interaction between dermal papilla cells and hair follicle epithelial cells,to understand the regulation of hair follicle growth cycle, and to find out the pathway of hair follicle reconsitution in vitro and in vivo. Method Human dermal papilla cells and hair follicle epithelial cells were cultured in the same or separated compartments.The cells were counted in different phases and the cell growth pattern was observed. Results The two kinds of cells could stimulate the proliferation each other.Human hair follicle epithelial cells could induce dermal papilla cells to aggregate and form dermal papilla－ like structure. Conclusion There is a mutual interaction between the epithelial cells and dermal cells of the hair follicle.The growth and development of hair follicle is regulated by the interaction of these cells.

0 05), respectively.Conculsion Transduction of NKG 5SV into NK cell can augment its cytotoxic activity by enhancing its cytolytic ability.

Objective To evaluate the value of prenatal gene diagnosis for thalassemia. Methods 128 fetuses suspected with thalassemia were performed amniocentesis or cordocentesis for gene diagnosis. Results No severe complications occurred during all procedures. 32 fetuses had normal genotype. 38 were heterozygous and 27 were homozygous of ?- thalassemia; 18 were heterozygous, 4 were homozygous and 9 were double heterozygous of ?-thalassemia. The types and frequencis of ?-thalassemia mutation were CD 41-42(47.5%), IVS-Ⅱ-654(42.5%), 17(A-T)(7.5%) and -28 (A-G)(2.5%) in turn. Pregnancies of 40 fetuses with severe thalassemia were terminated in time. Conclusions The screening and prenatal diagnosis of high risk fetus for thalassemia is safe, effective and accurate. It should be used as an obstetrical routine examination at the region with high thalassemia occurrence.

A new fusion expression vector, pED-P8-Hi-lys was designed and constructed. It includes four parts, a 20 peptide sequence of hirudin that can maintain anticoagulant activity, the C-terminus of asparaginase as a fusion partner, basic octopeptide (KRKRKKSR) that makes the fusion partner easy to remove, and the unique acid-labile aspartyl-prolyl bond. It was transformed into E. coli BL-21 and the fusion protein (AnsB-C-P8-Hi-lys) was expressed effectively as inclusion bodies after inducing by lactose. The objective peptide Hi-lys was purified by means of cell disruption, washing, ethanol precipitation, acid hydrolysis, and DEAE-cellulose 52 column chromatography. The antithrombin activity of the purified Hi-lys peptide was about 50 ATU/mg by thrombin activity assays.

Objective To compare the contents of functional outcome measurements for stroke. Methods The concepts of 14 kinds of functional measurement tools were coded matching with comprehensive ICF Core Set for stroke. Results The difference of measurement tools was displayed after coded with ICF. Conclusion The content comparison based on the ICF can ease the choice of measurement tools.

Objective To evaluate the clinical effect of light cure Dyractflow (LCDF) in pit and fissure enamel-plasty sealant technique (EST). Methods A total of 400 first permanent molars in 200 patients aged from 6 to 10 years were treated with LCDF in occlusal pit and fissure enamel-plasty sealant technique. The opposite permanent teeth treated with LCDF in occlusal pit and fissure conventional sealant technique (CST) were employed as the controls. The patients were followed up for 3 years. Results Follow-up for 3 years showed that there was no significant difference in preservation rate between the EST and the CST groups in the first year, but in 2-3 years, there was significant difference in preservation rate (P