OBJECTIVE:To study the effects of baicalin on human osteosarcoma MG63 cells apoptosis and the expression of MMPs. METHODS:Treated with 0(blank control),5,10,20,40,80,160,320 μg/ml baicalin for 24 h,the survival rate,the expression amount of MMP-2 and MMP-9 were detected,and IC50 was calculated. Cell apoptosis was observed. RESULTS:Com-pared with blank control group,after treated with baicalin,survival rate of MG63 cells decreased,while apoptotic amount in-creased,the expression amount of MMP-2 and MMP-9 decreased;there was statistical significance in the expression amount de-crease of MMP-2 and MMP-9 in MG63 cells after treated with 320 μg/ml baicalin(P<0.05);IC50 was(40.21±9.20)μg/ml,all responses were in concentration-dependent manner. CONCLUSIONS:Baicalin can inhibit the proliferation of MG63 cells,induce cell apoptosis,and inhibit the expression of MMP-2 and MMP-9 in cells under high concentration.

Pachychoroid spectrum diseases includes central serous chorioretinopathy (CSC),pachychoroid pigment epitheliopathy,pachychoroid neovasculopathy,and polypoidal choroidal vasculopathy,which share common characteristics,including focal or diffused increased choroidal thickness,choroidal hyperpermeability,and dilated choroidal vessels.These diseases are likely to represent a continuum of the same pathogenic process.Similar features and association among them suggest that they may have similar etiology.It is of great clinical significance to understand the composition and typical morphological changes of pachychoroid-related diseases and to explore its possible pathogenesis.

Objective To analyze molecular classification and male breast cancer (MBC) triggers,clinical characteristics in patients with MBC and evaluate the prognosis.Methods 36 MBC patients enrolled in the past 5 years were retrospectively analyzed.100 patients with women breast cancer (WBC) in the same period were randomly selected as control group.The primary pathological type of MBC was the infiltrating ductal carcinoma.According to the expressions of estrogen receptor (ER),progesterone receptor (PR),and the epidermal growth factor receptor 2 (Her-2),the MBC can be divided into 4 kinds of molecular subtypes,Luminal type A,Luminal type B,Her-2 type,and Basal-like type.Results The ratio of MBC patients with grade Ⅲ was lower in the Luminal group A than that in the Luminal group B.There were significant differences (x2 =1.197,P ＜ 0.05) between these two groups.Conclusion The incidence rate of MBC is low but the prognosis is poor.The primary pathological type is the infiltrating ductal carcinoma.The ratio of MBC patients with grade Ⅲ is lower in the common Luminal group A than that in the common Luminal group B.

Objective To observe the effects of shenfu injection (SFI) on the awakening quality of the patients with hepatitis B cirrhosis undergoing splenectomy under general anethesia. Methods Forty patients with hepatitis B cirrhosis and hepatic insufficiency (ASA classⅡ~Ⅲ) underwent splenectomy by general anesthesia. Patients were all sent into the post-anesthesia care unit (PACU) shortly after the operation with unconscious and no spontaneously breathing. They were randomly divided into two groups: SFI treatment group (Group SFI, n =20) and normal saline controlled group (Group NSC, n = 20). SFI group were treated with SFI (1 mL/kg, i. v.) in 10 minutes, and NSC group were treated with normal saline (1 mL/kg,i.v.). The time of eyes opening, extubation of tracheal catheter and the detention time of PACU were recorded. The heart rate (HR) and the average artery presses (MAP) were monitored at 4 time points: before SFI and normal saline administration, 5 min, 15 min, and 45 min after administration. The incidence of restlessness during the patients awakening period was also recorded. Results The time of eyes opening, extubation and the detention time of PACU of SFI group show no significant difference compared with the NSC group (P > 0.05). SFI and normal saline intravenous injection did not cause significant changes on HR and MAP at the time of 5 min , 15 min and 45 min compared to the time of before administration (P > 0.05). The incidence of restlessness during the patients resuscitation period in SFI group were lower than in NSC group (P < 0.05). Conclusion Shenfu injection can effectively improve the awakening quality and decrease the incidence of restlessness of the patients with hepatitis B cirrhosis undergoing splenectomy under general anesthesia during the awakening period in PACU.

0 05), respectively.Conculsion Transduction of NKG 5SV into NK cell can augment its cytotoxic activity by enhancing its cytolytic ability.

BACKGROUND: Traditional cancellous bone porosity detection methods such as direct method,gas expansion method,mercury injection method,density and osmotic suction measurement have many disadvantages,such as low efficiency,labor-intensive and complexity.OBJECTIVE: To process cancellous bone chips using MATLAB image processing method to calculate cancellous bone porosity.METHODS: Using MATLAB image processing method,the stained cancellous bone images were subjected to uneven lighting removal,image enhancement,binarization,removing interference objectives to obtain binary two-dimensional matrix.RESULTS AND CONCLUSION: Slice image was composed of bone and pore space.The slice image was segmented to calculate its porosity.This solves the problem of the inequality of light intensity and other interferences in image information that affect the statistics.The image processing and analysis methods of bone slices were performed using MATLAB,which can be used for cancellous bone porosity measurements and be used to explore the distribution and variation of porosity.Selecting different regions to calculate the different porosity of each area can be used to analyze the distribution of the pore.This method provides a method for the study of trabecular bone structure.

Objective To construct a plasmid expression vector coding for the short hairpin RNA(shRNA) targeting Twist mRNA.Methods Two plasmid expression vectors coding for shRNA targeting 777 and 845 of Twist gene sequence and a control vector containing random DNA fragment were constructed.The recombinant plasmids were identified by PCR,and then transfected separately into bladder cancer cell line T24.The Twist gene silencing effect was detected by RT-PCR and Western blotting.Results The expected band of 400 bp was amplified from the plasmids coding for shRNA by PCR.By DNA sequencing,it was the same with the insertion element as with the shRNA of synthetic.Transfection of T24 cells expressing Twist gene with the shRNA plasmids resulted in inhibition of Twist mRNA and protein expressions by 90% and 86%,respectively.The shRNA1 had the most obvious effect in Two types of plasmids interference.Conclusion The plasmid expression vectors coding for shRNA targeting Twist mRNA have been constructed successfully,of which pGenesil-shRNA1 most effectively silences Twist gene in T24 cells.

Introduction of problem-based learning (PBL) teaching is the demand of teaching reform.Teaching and research section of physiology in Chongqing medical university implemented PBL teaching throughout the whole process of clinical medicine undergraduate education.The overall quality of students and the teaching quality were improved by constructing a new mode suitable for physiology teaching,focusing on training students' active learning ability and stimulating students' enthusiasm for learning.

Objective To investigate the role of mitogen-activated protein kinases (MAPK)/extracellular signal regulated kinase1/2 (ERK1/2) signal transduction pathway in regulating the expression of aquaporin 3 (AQP3) in human amnion epithelial cells.Methods Primary cell culture of human amnion epithelial cells deriving from amnion of term pregnancy with normal amniotic fluid volume (AFV) or isolated oligohydramnios was conducted in the Second Affiliated Hospital of Wenzhou Medical College from January to November 2011.Either group included 10 elective cesarean cases.The primarily cultured cells were treated with different concentrations (0,5,10,20 and 40 mol/L) of ERK inhibitors (U0126) for 12 h,and then the optimal concentration of U0126 which resulted in the lowest expression of phospho~ERK1/2 (p-ERK1/2) was added for different durations(0,2,6,12 and 24 h).Immunocytochemistry was used to detect the localization of AQP3 and Western blot analysis was used to examine the expression of total ERK1/2,p-ERK1/2 and AQP3 in human amnion epithelial cells.Statistical analysis was performed by t-test and one-way ANOVA.Results (1) Compared with those in normal AFV group,p-ERK1/2 and AQP3 expression in human amnion epithelium cells decreased in oligohydramnios group,respectively (p-ERK1/2:3.46 ± 0.33 and 2.46±0.25;AQP3:2.34 ± 0.18 and 1.56±0.10,t=9.243 and 13.292,P＜0.01).(2) In oligohydramnios groups,after treated with different concentrations of U0126,the expressions of total ERK1/2 did not change (F=0.365,P＞0.05).The expression of p-ERK1/2 and AQP3 in 5,10,20,40 μmol/L U0126 (p-ERK1/2:0.96±0.16,1.12±0.13,0.98±0.17 and 1.02±0.26; AQP3:1.10±0.09,1.12±0.08,1.13±0.06 and 1.11±0.06) were all significantly lower than those in 0 μmol/LU0126 group (p-ERK1/2:2.46±0.25; AQP3:1.56±0.10,P＜0.05).However,the expression of p-ERK1/2 and AQP3 showed no significant difference among 5,10,20,and 40μmol/L U0126 groups (P＞0.05).The optimal concentration of U0126 was 5 μmol/L.After treated with 5 μmol/L U0126 for 2 h,the expressions of p-ERK1/2 and AQP3 (1.27±0.29 and 1.44±0.12)were lower than those after treated for 0 h (2.55±0.12 and 2.15±0.09,P＜0.05).However,there was no significant difference among groups treated for 2,6,12 and 24 h.Therefore,the optimal treatment time was 2 h.(3) The expression of AQP3 was distributed in both cell membrane and cytoplasm in amnion epithelial cells with normal amniotic fluid volume or isolated oligohydramnios,but mainly in cytoplasm.U0126 did not change the localization of AQP3 expression.Conclusions U0126 inhibits the phosphorylation of ERK1/2 and expression of AQP3 of women with oligohydramnios,indicating that the MAPK/ERK1/2 signal transduction pathway might regulate the expression of AQP3 in human amnion epithelial cells,and therefore affect the balance of amniotic fluid volume.