Objective To investigate the effect of Xinmailong injection combined with Qiliqiangxin capsule on serum BNP , NF-kappa B and cystatin C in the treatment of chronic heart failure in the elderly .Methods 78 cases of elderly patients with chronic heart failure were selected and randomly divided into two groups according to the random number table, 39 cases in each group.All patients received basic treatment, the control group received standard treatment, and the study group received more with Xinmailong injection combined with Qiliqiangxin capsule,and levels of serum B-type natriuretic peptide(BNP), cystatin C(CysC) and NF-κB were compared, left ventricular function and 6 min walking test were detected and the clinical curative effect were compared after the treatment.Results Compared with before treatment, levels of serum BNP, CysC and NF-κB in 2 groups decreased(P<0.01), left ventricular ejection fraction(LVEF) increased(P<0.05), left ventricular end-systolic volume(LVESV) reduced(P<0.05), 6 min walking distance increased(P<0.01), scores of symptom decreased(P<0.01), compared with the control group, levels of serum BNP, CysC and NF-κB in study group were lower, LVEF were higher ( P<0.05 ) , levels of LVEDV and LVESV were lower ( P<0.05 ) , scores of 6 min walking distance were higher(P<0.01), scores of symptom were lower (P<0.01), the effective rate was higher(P<0.05).Conclusion Xinmailong injection combined with Qiliqiangxin capsule in the treatment of chronic heart failure in the elderly was effective , and it can improve left ventricular function, the possible mechanism related to the decrease of BNP, CysC and NF-κB levels.

OBJECTIVE:To investigate the characteristics and regularity of ADR in secondary-level hospitals and above in Xi’an in order to provide reference for clinical drug use. METHODS:During Oct. 2012-Jun. 2014,1 372 ADR cases reported in 36 hospitals at secondary-level or above in Xi’an were analyzed retrospectively. RESULTS:Among 1 372 cases of ADR reports,the incidence of ADR in the above 60 age group occupied the highest percentage,being 29.67%(407 cases);ADR reports were most likely caused by antibacterial drugs,accounting for 45.77%,followed by TCM injections(15.82%)and circulatory system drugs(9.55%);the largest number of ADR reports was caused by injections(1 210 cases,88.19%);the main types of ADR were lesion of skin and its appen-dents,accounting for 44.16%. The most number of ADR was induced by Levofloxacin injection,involving 95 cases. CONCLU-SIONS:Mastering the characteristics and regularity of ADR help managers to standardize clinical rational drug use and ensure patients’ medication safety.

OBJECTIVE To evaluate the clinical characteristics of chronic severe-degree hepatitis B complicated by aspergillosis and its susceptive factors and prevention and treatment.METHODS The clinical data of 3 patients with chronic severe-degree hepatitis B complicated by aspergillosis were analyzed retrospectively.RESULTS From 23 patients with chronic severe-degree hepatitis B complicated by fungus infection,there were 3 patients complicated by aspergillosis and died.The susceptive factors included poor immunity,incorrect use of antibiotis and invasive operation.CONCLUSIONS Patients with chronic severe-degree hepatitis B complicated by aspergillosis have poor progress and prognosis. The effective preventive methods are treating underlying disease actively,using antibiotis correctly,reducing or avoiding invasive operation and disinfecting air regularly.

A new fusion expression vector, pED-P8-Hi-lys was designed and constructed. It includes four parts, a 20 peptide sequence of hirudin that can maintain anticoagulant activity, the C-terminus of asparaginase as a fusion partner, basic octopeptide (KRKRKKSR) that makes the fusion partner easy to remove, and the unique acid-labile aspartyl-prolyl bond. It was transformed into E. coli BL-21 and the fusion protein (AnsB-C-P8-Hi-lys) was expressed effectively as inclusion bodies after inducing by lactose. The objective peptide Hi-lys was purified by means of cell disruption, washing, ethanol precipitation, acid hydrolysis, and DEAE-cellulose 52 column chromatography. The antithrombin activity of the purified Hi-lys peptide was about 50 ATU/mg by thrombin activity assays.

With the gradual development of reformation,the traditional notion of service that only cares about the state of illness but ignores the state of patients is challenged by different factors violently in the hospital.Nowadays,with more concerns on People First,the situation,neglect of patients,must be accordingly changed in hospitals and more em?phasis should be put on the humane care during treatment in order to cure patients’diseases successfully.

AIM　The purpose is to construct D-hydantoinase genetic engineering strain for the purpose of the industrial production of D-p-hydroxyphenylglycine. METHODS　D-hydantoinase gene was created from Pseudomonas putida 9801 by PCR technique and inserted into pMD18-T vector. The recombinant plasmid was transformed into several Escherichia coli strains. The positive transformants with D-hydantoinase activity were obtained by the two step screening, digoxigenin DNA labeling in situ hybridization and D-hydantoinase activity assay. RESULTS　The D-hydantoinase activity of the genetic engineering strain E.coli BL21/pMD-dht was 1700 U*L-1 and increased as high as 8 times compared with those of wild-type strain Pseudomonas putida 9801. The subunit molecular weight of recombinant D-hydantoinase was about 53 kDa measured by SDS-PAGE. The amount of the recombinant D-hydantoinase was about 20 percent of total bacterial soluble proteins. CONCLUSION　The genetic engineering strain E.coli BL21/pMD-dht possesses the initial industrial production prospects.

Objective:To draw a mapping knowledge domains of research status and trend in counseling and psychotherapy field from 2001 to 2014 by Bicomb software.Methods:CNKI,Wan Fang,VIP were searched and re-trieval time ranged from January 1,2001 to December 31,2014.Bicomb and SPSS20.0 software were applied to conduct co-word matrix,similarity matrix and multidimensional scaling.Results:Setting 30 and more as the fre-quency standard,60 high frequency keywords were abstracted after analyzing 6546 articles.Cluster analysis showed that studies about counseling and psychotherapy mainly focus on seven categories including the effectiveness of counseling and psychotherapy,children and adolescents'mental health and counseling,the localization of counseling and psychotherapy,the process of counseling and psychotherapy,counseling and treatment principles,relationships of psychotherapy and ideological work in colleges as well as theories and practices in counseling and psychotherapy field.The mapping knowledge domain showed that four aspects of high frequency keywords in counseling and psy-chotherapy field basically correspond to the 7 categories made by cluster analysis.Conclusions:The present study demonstrates that psychological counseling and treatment development of our country is still in the stage of investi-gating effect.Future researches should attach importance to the integration research of psychological counseling and ideological and political education in colleges,the rise and development of internet psychological counseling and the development of children and adolescent mental health.

Objective To establish the rapid purification of Coxsackievirus A16 using ultracentrifugation .And To prepare and i‐dentify the neutralizing monoclonal antibody against CA16 .Methods The CA16 culture supernatant was harvested and then con‐centrated by 100K capsule .The concentration of CA16 was purified by cesium chloride ultracentrifugation .Purification of CA16 were identified by transmission electron microscopy .BALB/c mice were immunized with inactivated CA16 .Spleen cells were harves‐ted and fused with SP2/0 myeloma cells ,hybridoma cell strain secreting mAb against CA16 were objected to screening .Character‐ization of the prepared mAb were analyzed by ELISA and microneutralization assay .Results The purified CA16 method of cesium chloride gradient ultracentrifugation was established ,TEM analysis was showed that CA16 particles have icosahedral structure ,the diameters of the viral particles were approximately 20-30 nm .Two hybridoma cell strains secreting mAb against CA16 were ob‐tained ,the subtypes of two mAbs were IgG2a ,the binding titers of Anti/CA16/5 and Anti/CA16/10 were 103 and 104 respectively . Neutralizing titer of the two mAbs were 1∶256 and 1∶1 024 respectively .Conclusion Establishment method of cesium chloride gradient ultracentrifugation was performed to purify CA16 ,the two mAbs with neutralizing ability to against CA16 may become ap‐plication of treatment and vaccine .

Objective To determine the relationship between acceptance of disability and posttraumatic stress response in patients with brachial plexus injury. Methods Total 160 patients with brachial plexus injury were recruited and investigated with the Impact of Event Scale-Revised (IES-R) and the Acceptance of Disability Scale (ADS). Results The total score of ADS was (79.07±11.99) which showed medium level of acceptance of disability. The total score of IES-R was 4-66 (33.51±14.41), which showed that most of the patients suffered from posttranmatic stress response. Significantly negative correlation was found between acceptance of disability and posttraumatie stress response(r=-0.480, P<0.001). Conclusions Nurses should pay more attention to the acceptance of disability and posttranmatic stress response in patients with brachial plexus injury, and provide appropriate health education and effective psychological intervention to improve the patients' mental health and quality of llfe.

The ancient documents and modern literature in recent 15 years have been retrieved and reviewed on acupuncture treatment of migraine.This article has analyzed syndrome and disease differentiation,acupoint-selection and combination as well as therapies and manipulations,and further provided clinical application thoughts based on therapeutic evaluation of the above aspects.