Objective To evaluate the endometrium receptivity of endometriosis (EMs) infertile patients, and observe the influence of TCM treatment to endometrium receptivity and IVF-ET pregnancy outcome. Methods Fifty EMs infertile patients were randomly divided into two groups. The reatment group (30 cases) kept taking TCM for 3 month continuously before IVF-ET embryo transplantation, and the control group (20 cases) received no treatment, with 30 healthy women as normal control. Using transvaginal color doppler ultrasound to detect and compare the endometrial thickness, endometrium and endometrial blood flow distribution types, and uterine artery blood flow parameters in window transvaginal stage. Results There was no difference in endometrial thickness between the EMs infertility patients and the normal group, and the endometrial thickness also had no obvious changes before and after treatment (P>0.05). RI and PI of EMs infertility patients were significantly increased, compared the with normal control group (P<0.05). In treatment group, the persentage of type Ⅰ blood distribution was 23.33%(7/30), in control group was 25.00%(5/20), and 66.67%(20/30) in the normal group, significantly higher than that of the EMs infertility patients (P<0.01). Uterine artery blood flow parameters RI, PI value were significantly decreased after treatment in treatment group, endometrium and endometrial blood flow type Ⅰ distribution increased to 60.00%(18/30). The rate of normal pregnancy was 46.67% (14/30) in treatment group, and control group was 30.00% (6/20). Conclusion EMs may affect uterine artery hemodynamic status and endometrium receptivity. TCM treatment can improve the endometrium receptivity of EMs infertile patients and improve the success rate of IVF-ET.

Objective:To investigate the clinical application and adverse events of vinpocetine injection. Methods:The application of vinpocetine injection in the patients in neurology department during December 2013 and December 2014 in a hospital was statistically analyzed. The adverse reactions of the injection reported in the professional literatures and relevant documents were also retrieved and statistically analyzed. Results:The infusion concentration of vinpocetine injection for 363 patients was more than 0. 06 mg·ml -1 ,and 3 cases of adverse reactions appeared with the main symptoms of rash and drug fever. Among 28 published literatures,19 articles were with the infusion concentration of vinpocetine injection above 0. 06 mg·ml-1 and 8 articles reported adverse reactions in varying degrees. Conclusion:Clinicians should pay attention to the instructions in the clinical course of medication in order to improve the safe and rational use of drugs.

Objective To analyze the changes of distribution and drug resistance of isolated pathogens for ventilator-associated-pneumonia (VAP) in seven intensive care units from January 2013 to December 2015,so that provides information for clinical antibiotic treatment.Methods Collected the patient records diagnosed with VAP to get the distribution and drug resistance of isolated pathogens for VAP in ICU from January 2013 to December 2015.Compared the distribution of isolated pathogens for VAP among three year with Chi-square tests,and compared the drug resistance of main isolated pathogens with Nonparametric tests.Results 177 patients were diagnosed with VAP from January 2013 to December 2015.A total of 192 strains of pathogens were isolated from the VAP patients in ICU.Among which the gram-negative bacteria were dominant.It took up 90.6％.There was no significant statistical difference for the distribution of pathogens (x2 =2.372,P=0.668＞0.05).Analyzed the drug resistance rate and trends for the primary pathogens like Acinetobacter Bauman,Pseudomonas aeruginosa and Staphylococcus aureus,among which,the drug resistance of Acinetobacter Bauman from 2013 to 2015 made statistical difference.Conclusion The major pathogen isolated from VAP patients was gram-negative bacteria from January 2013 to December 2015,especially for Acinetobacter Bauman.Drug resistance of Acinetobacter Bauman was serious.Should administrated appropriate to treat the VAP infection resulted from Acinetobacter Bauman.

Objective To improve the imaging diagnosis and differential diagnosis of non-epithelial ovarian tumor.Methods 34 patients with non-epithelial ovarian tumor confirmed by pathology were analyzed retrospectively.The image features of each patients were analyzed and compared with surgical pathology.Results The results exhibited 13 cases of teratoma,6 cases of ovarian granulosa cell tumor, 6 cases of ovarian fibroma, 5 cases of thecoma, 2 cases of dysgerminoma,and 2 cases of endodermal sinus tumor.All kinds of non-epithelial ovarian tumors had its imaging manifestations and clinical features.Conclusion Non-epithelial ovarian tumors have certain characteristic CT and MR performance,and combining the onset age,clinical symptoms and biochemical examination,can make the qualitative diagnosis.

Objective To investigate the distribution and antibiotic resistance profile of clinical isolates in the First Hospital of Qiqihar during 2015.Methods Antimicrobial susceptibility test was carried out according to a unified protocol using automated system from January 1,2015 to December 31,2015.The results were analyzed with WHONET 5.6 software according to the 2014 breakpoints of Clinical and Laboratory Standards Institute.Results A total of 5 162 clinical isolates were collected,of which 28.1％ (1 450/5 162) were gram-positive cocci and 71.9％ (3 712/5 162) were gram-negative bacilli.About 36.5％ (255/698) ofS.aureus isolates and 81.4％ (180/221) of coagulase negative Staphylococcus isolates were resistant to methicillin.No S.aureus and coagulase negative Staphylococcus isolate were found resistant to vancomycin or linezolid.Enterococcus isolates showed low resistance to vancomycin and linezolid.One strain of E.faecium was found resistant to vancomycin.ESBLs were produced in 39.9％ (298/747) ofE.coli,26.1％ (294/1 127) ofKlebsiella spp.,and 15.6％ (12/77) ofP mirabilis strains.The Enterobacteriaceae strains were less resistant to imipenem,beta-lactam/beta-lactamase inhibitor combination and amikacin.About 36.6％ (163 / 445) of A.baumannii isolates and 1.8％ (13/715) of P.aeruginosa isolates were extensively drug-resistant strains.Conclusions Antibiotic resistance poses a serious threat to clinical practice,to which more attention should be paid.Clinical microbiology lab should make more efforts to provide better support to clinical therapy.

Objective: To investigate whether Vigconic VI-28 capsule, a formulated traditional Chinese medicine containing radix Ginseng, cornu cervi pantotrichum and semen cuscutae, can assist tumor chemotherapy in a mouse model. Methods: Female C57BL/6 mice were s. c. injected with viable Lewis lung cancer ( LLC) cells (106 cells/mouse) . The mice were then treated with cyclosposphamide (Cyp, 40 mg/kg bodyweight, once every other day). One group was intra-gastrically given 2% VI-28 (0.5 ml/mouse, every other day) during the course of the chemotherapy. By day 28, the mice were sacrificed and their thymic indices and tumor indices were calculated and compared. Splenocytes were collected for analysis of their immunological status. Histological study was carried to examine the solid tumors. Results: Fourteen days after the injection of LLC cells, solid tumors developed in most of the animals, reaching 1 ~ 1.8 cm diameters by 28 th day Compared with mice of the LCC + Cyp group, thymus glands from the LLC + Cyp + VI-28 group were significantly heavier. Splenocytes of the same group responded better to ConA stimulation in vitro. Histochemical examination of the tumor tissues revealed that tumors of the Cyp + VI-28 group were better differentiated (less aggressive) than that of the Cyp group. Conclusion: Vigconic VI-28 capsule can promote recovery of immune system in mice undergoing chemotherapy and help Cyp to control the growth of tumor cells in vivo.

Objective:To analyze the consistency of HER-2 expression among endoscopic biopsy and radical operation specimens of gastric adenocarcinoma and to investigate the clinical application value of HER-2 detection in trastuzumab treated patients. Methods:From March 2013 to February 2014, 167 patients from Shanghai Changhai Hospital were diagnosed with gastric adenocarcinoma using endoscopic biopsy specimens. The corresponding surgical specimens were collected for pathological analysis. The relevant clinical and pathological data were collected. HER-2 protein expression of endoscopic biopsy specimens was detected by immunohistochemistry (IHC). HER-2 protein expression and gene amplification status of the corresponding tumor resection specimens were detected by IHC and fluorescence in situ hybridization (FISH). Results were analyzed for clinicopathological characteristics. Results:Among the 167 cas-es, 18 cases (10.8%) were HER-2 positive, including 10 cases showing IHC3+and 8 cases showing IHC2+with FISH positive. The consis-tency rate result among endoscopic biopsy and surgical operation specimens was 82%. Excluding the cases showing IHC2+, the true positive rate and the true negative rate were 73.3%and 97.0%, respectively. Conclusion:HER-2 detection of endoscopic biopsy speci-men by IHC shows great predictive value. The main reason for the difference of surgery and biopsy specimens is the heterogeneity of tumor expression. Increasing the number of specimens and combined testing with FISH are important methods to reduce misjudge-ment.

Objective To describe the quality of life of breast cancer patients and explore the risk factors for their quality of life (QOL). Methods QOL of 260 patients with breast cancer were studied using FACT-B, SCL-90, Social Support Rating Scale and multiple linear regression models to study the main factors influencing the QOL of patients with breast cancer on functional scales (physical, social, emotional, role). Results Economic conditions (β =2.024, P =0.003), emotion regulation ability (β =4.993, P =0.000) and self-evaluation of marital quality (β =-3.954, P =0.001) were associated with total QOL scores, emotion regulation ability (β =0.987, P =0.008), the number of emergency incidents (β=-0.866, P =0.011) were associated with physical domain scores, economic conditions (β =1.016, P =0.000), marital status (β =-1.018, P =0.041), emotion regulation ability (β =0.971, P =0.008), self-evaluation of marital quality (β =-1.382, P =0.001) were associated with social and family domain scores, emotion regulation ability (β =1.094, P =0.000), self-evaluation of marital quality (β =-0.892, P =0.006) were associated with emotional domain scores, regional factors(β =1.653, P =0.008), emotion regulation ability (β =0.962, P=0.01), self-evaluation of the QOL (β =-1.705, P =0.000) were associated with function domain scores. Conclusion Guiding the patients to have a correct understanding of the breast cancer, strengthening their confidence of life, increasing physical activity, having a good family environment and necessary social welfare so that improving the QOL of the patients.

Objective To know the medical effectiveness of health management on pilots with chronic fatigue syndrome during the course of convalesce. Methods Divided 97 pilots with chronic fa-tigue syndrome according to related diagnostic criteria, and then divided them into the experimental group (47 cases) and the control group(50 cases) randomly. Routine nursing cares were used in the control group, while health management was used in the experimental group in addition. Compared the stares of re-lated symptom easing between the two groups by BFI, FS and FAI scales. Results The chronic fatigue syndrome-related symptom were allieviated in both the two groups after the convalesce, while the allievia-tion degree in the experimental group was significantly. Conclusions Health management during the course of convalesce can effective allieviate the symptom of pilots with chronic fatigue syndrome, and then promote their rehabilitation.

BACKGROUND:Tranditional Chinese medicine,which possesses anti-oxidation properties,can promote directional differentiation of mouse bone marrow mesenchymal stem cells(BMMSCs)into nerve cells.Salidrosides,as the effective constituent of Rhodiola,have strong anti-oxidation function.OBJECTIVE:To investigate the molecule mechanism of salidrosides induced differentiation of mouse BMMSCs into nerve cells.METHODS:When in vitro cultured BMMSCs reached 80% confluency,the cells were assigned into 3 groups.Cells in the control group were cultured by complete culture medium;those in the induction and positive control groups were cultured by complete culture medium adding 20 mg/L salidrosides or 0.1 mg/L nerve growth factors(NGF).The related gene and protein of nerve cells were detected using RT-PCR and Western blot method at 12 hours after culture.After that,the cells in the induction group were divided into 3 groups,the blocking agents EGTA(Ca~(2+) chelator),Nifedipine(L-type Ca~(2+) channel blocker)and LY294002(IP3 receptor blocking pharmacon)were applied to block the cellular Ca2* signal pathway respectively for 12 hours.RT-PCR and Western blot methods were used to study the signal transduction of the salidrosides.RESULTS AND CONCLUSION:①The expression of neuron specific enolase(NSE),B-Tubulin III,Nurri mRNA could be found ir the induction and positive control groups,instead of the control group;The expression abundance of the positive control group was smaller than that of the induction group.The expression abundance of GFAP mRNA was very low in each group,but the c-fos mRNA was expressed abundantly in the induction group.②Compared with the positive control group,the induction group could promote the NSE expression obviously,which was no expressed in the blank control group.?The expression of NSE and Nurri were conspicuously down-regulated when the Ecto Ca~(2+) and L-type Ca~(2+) channel and IP3 receptor were blocked respectively.Salidrosides can induce the differentiation BMMSCs into nerve cells. Ca~(2+) signaling and IP3 dependent Ca~(2+) signaling pathway play an important role in transduction salidrosides signal in BMMSCs differentiation.