Objective To investigate the characteristics of serum oxidized low density lipoprotein (ox-LDL) and traditional cardiovascular risk factors of premature coronary artery disease (PCAD). Methods Sixty-six patients with coro-nary atrery disease (CAD) confirmed by angiography were selected to be PCAD group, and 56 patients with normal coronary artery confirmed by angiography were included in control group. The serum levels of ox-LDL, high density lipoprotein choles-terol (HDL-C), triglyceride (TG), creatinine (SCr), uric acid (UA), other serum biochemical indicators and differences of the proportions of male, hypertension, diabetes, smoking history and family history of CAD were compared between two groups. Results (1)There were higher serum values of ox-LDL, TG, SCr, UA, fasting blood glucose (FBG) and proportion of male pa-tients in PCAD group than those of control group (P＜0.05), but there was a lower plasma level of HDL-C in PCAD group than that of control group (P＜0.05). There were significant differences in other indicators between two groups. (2) There were positive correlations between UA, SCr and ox-LDL ( rs were 0.243 and 0.186 respectively, P were 0.006 and 0.034 respective-ly). (3)The logistic regression analysis showed that ox-LDL, HDL-C and SCr were influential factors to PCAD(OR were 1.155, 0.143 and 1.052 respectively). Conclusion Elevations of ox-LDL, HDL-C and SCr are independent predictors of PCAD.

Objective To observe the inhibitory effects of sub-MIC matrine alone and in combination with erythromycin on Staphylococcus epidermidis biofilms and their influences on morphological changes of the biofilms.Methods Minimum inhibitory concentrations (MIC) of matrine and erythromycin against Staphylococcus epidermidis were determined by the serial dilution method,antibacterial activity of matrine combined with erythromycin against planktonic S.epidermidis was evaluated by the checkerboard method.S.epidermidis biofilms were constructed in vitro,XTT reduction assay was used to evaluate influences of sub-MIC matrine alone and in combination with erythromycin on metabolism and adhesion of S.epidermidis biofilms,and scanning electronic microscope(SEM) was applied to observe the morphological and the structural changes of the biofilms.Results The MIC of erythromycin to S.epidermidis was 7.8125 μg/ml,while the MIC of matrine was greater than 1000 μg/ml,besides,a synergistic effect between erythronmycin and matrine on planktonic S.epidermidis was shown (FIC＜0.5).The sub-MIC matrine had no significant inhibitory effect on adhesion of S.epidermidis,and also the combination of the two agents was better than was used alone.However,the sub-MIC matrine had inhibitory effects on metabolism and morphology of S.epidermidis biofilms,and the combination of the two agents was weaker than was used alone.Conclusion Both the sub-MIC matrine and erythromycin had a significant inhibitory effect on S.epidermidis biofilm formation.Combination of the two agents showed synergistic effects on plankton and adhesion of S.epidermidis,but showed no synergistic effect on metabolism and morphology of the biofilms.

OBJECTIVETo provide a new therapeutic approach for Staphylococcus epidermidis biofilm-associated infections by the study of inhibitory effect of andrographolide (AG) on S. epidermidis biofilm.

METHODS. epidermidis biofilms were set up in vitro, erythromycin was acted as the positive control agent, XTT reduction assay was used to evaluate AG on the initial adhesion of S. epidermidis and bacterial metabolism within biofilm, microscope was applied to observe biofilm morphology, and Congo red assay was used to detect polysacchatide interc-ellular adhesion (PIA)formation when exposed to AG.

RESULTAG showed inhibitory effects against the initial adhesion of S. epidermidis at concentrations of 1 000,100, 10 mg x L(-1), respectively,and inhibited metabolism of biofilm bacteria at the concentration of 31.25 mg x L(-1), and exhibited significantly inhibition against the biofilm morphology at the concentration of 250 mg x L(-1), while did not display inhibition against PIA formation at the concentration of 10 mg x L(-1).

CONCLUSIONAG could remarkably inhibit biofilm formation of S. epidermidis, although it was less potent than erythromycin.

Bacterial Adhesion ; drug effects ; Biofilms ; drug effects ; Diterpenes ; pharmacology ; Dose-Response Relationship, Drug ; Erythromycin ; pharmacology ; Staphylococcus epidermidis ; drug effects ; physiology

Purpose/Significance To clarify the construction mode and key factors of data sharing in Chinese medical alliances,and to provide references for promoting data sharing in medical alliances.Method/Process Taking children's medical alliance of Yangtze River Delta as an example,Delphi method and qualitative interview method are used to explore the key contents and effect evaluation of specialty alliance construction,and the key factors of specialty alliance data sharing are defined based on the technology-organization-environment framework;SWOT analysis model is used to propose development strategies.Result/Conclusion Member hospitals are less satisfied with the construction effect of information interconnection.Key factors for realizing data sharing in specialty alliances include:support from government administrative departments,uniform and systematic compatibility of information standards,information security emergency response,patient privacy protection and informed consent,willingness of department directors to share,and internal communi-cation and compensation mechanism of alliances.