Objective To observe the clinical treatment effects of Qingkailing injection on Alzheimer's disease.Methods 84 patients with AD were randomly divided into a control group and a treatment group (n=42 cases,per group). The patients in both groups orally took 5 mg of donepezil hydrochloride every night. The Qingkailing injection of 60 ml was administrated by intravenous drip in addition to oral uptake of donepezil hydrochloride to the treatment group. The total treatment period of both groups was 20 weeks. The MMSE, ADL score, HS-CRP, IL-6, MDA and ox-LDL content and the clinical treatment results and differences in the two groups before and after the treatment were observed.Results After treatment, the serum HS-CRP (4.18 ± 0.38 mg/Lvs. 5.78 ± 0.53 mg/L,t=15.900), IL-6 (34.28 ± 4.33?g/L vs.44.75 ± 4.99?g/L,t=10.270), MDA (6.04 ± 0.98?mol/L vs.7.43 ± 0.69?mol/L,t=7.516), ox-LDL (62.87 ± 7.65?g/L vs.102.56 ± 9.81?g/L, t=26.677) content of the treatment group were significantly decreased than the control group (P<0.01). The total effective rate between the treatment group and the control group had significance difference (83.3%vs.  61.9%;χ2=4.850,P=0.028). The MMSE score in both groups after the treatment were higher than those in the same group(t value were 8.036, 7.475, respectively,P<0.01), ADL scores in both groups after treatment were lower than those before treatment(t value were 7.818, 5.960, respectively,P<0.01), but no significance difference between the two groups(t value were 1.633, 1.710,respectively,P value were 0.106, 0.091, respectively).Conclusion The treatment effect of Qingkailing injection on Alzheimer's disease was sound.

One of the hallmark features of children with autism is language barrier,which mainly manifested with lack of visual contact and attention,imitation speech,broad pronunciation problems,poor understanding abilities to speech and abstract questions,deficiency of logicality,spoken language disorder,confusion about the usage of pronouns such as you,I and she or he,etc.According to the basic principles of language training,visual perception tracing,vision contact,cultivation of communication desire,auditory and understanding training can all be used to make children with autism to understand the relationship between pronunciation and words,try to pronounce syllables,make good preparation for valid pronunciation in the early stage of language training.Respiration training,point massage of oral surface and valid usage of pronunciation organs' practice can help them to say phrase and simple sentences gradually.They can say a sentence correctly and achieve the destination of grasping language through learning decorative words and enhancement of understanding and logic abilities.Very efficient result could be approached through correct evaluation,found existing problems and using correct training methods during language training of children with autism.

Objective To understand the serological detection of suspected measles cases in Xicheng District of Beijing,and provide basis for monitoring and prevention of measles.Methods Detection results of 200 blood speci-mens and age distribution of suspected measles cases in Xicheng District of Beijing in 2011 -2014 were analyzed. Results The positive rate of serological detection of suspected measles cases in 2011-2014 was 32.50%(65/200);positive rates in each year were 12.00%,4.35%,33.90%,and 44.09% respectively;there was significant differ-ence in the positive rate of serological tests in different years(P <0.001).Of 200 cases,65 were <5 years old,and 97 were >24 years old,positive rates of serological detection were 35.38% and 34.02% respectively;31 cases (15.50%)received vaccination of measles;vaccine coverage rate in IgM antibody positive cases was lower than IgM antibody negative cases (3.08% vs 21 .48%,P <0.001 ).Conclusion Positive rate of serological detection de-creased in 2012,then increased year by year,for children under 5 years,measles are controlled by immunization with measles vaccine,in order to achieve the goal of eliminating measles effectively,control and prevention of adult measles is of great significance.

Focusing on such problems as relative isolated information resource,bad cooperation among project members,complex project management and process,and totally low efficiency,we put forward a based-on-Web cooperation and management mode for scientific research.This mode is convenient for project management,fulfills the high-performance share within the project members,increases the management efficiency and pushes about the information process for cooperation management of scientific research.

Objective To analyze and study the relationship between plasma adhesion molecules,free amino acids and ovarian cancer.Methods A total of 67 patients with ovarian cancer in our hospital during the time of March 2015 to May 2016 were selected as the observation group,and 67 healthy women at the same time were selected as the control group.The plasma adhesion molecules and free amino acids levels of two groups were detected and compared,The detection levels of observation group with different stages and degree of differentiation of ovarian cancers were compared.The relationship between plasma adhesion molecules,free amino acids and ovarian cancer were analyzed by the Logistic analysis.Results The plasma adhesion molecules levels of observation group were all higher than those of control group (P ＜ 0.05),the plasma free amino acids levels were all lower than those of control group (P ＜ 0.05),and the detection levels of observation group with different stages and degree of differentiation of ovarian cancer plasma adhesion molecules and free amino acid levels had significant differences (P ＜ 0.05).The Logistic analysis showed that the plasma adhesion molecules and free amino acids had close relationship to the ovarian cancer (all P ＜ 0.05).Conclusions The plasma adhesion molecules and free amino acids of the patients with ovarian cancer show abnormal expression state,and the expression levels of patients with different stages and degree of differentiation of ovarian cancer have certain differences,so the detection value of those indexes in the patients with ovarian cancer is higher.

Objection To study the effect of letrozole on EM rat models and influence on the reproductive system. Methods Surgically transplanted autologous uterine tissues to ectopic site beside the uterines in rats were used as animal models to study endometriosis. 20 EM model rats were random divided into letrozole-treated group and saline solution-treated control group. The change of ectopic lesion volume in each group was compared before and after treatment. Apoptotic cells were assessed by the terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick-end labeling (TUNEL) assay, and the uterian horn and ovary were weighted and observed by optical microscopy to study the change of morphology. Results The volumes of the endometriotic tissues of letrozole group reduced more than that in control group[ ( 28. 75 ± 2.28 )mm3 vs ( 108.39 ±9. 98)mm3, P <0.01 ]. The apoptotic rate in letrozole group [ (5.52 ±2. 81 )% ]was higher than control group[ (2.11 ± 1.70)%, P <0. 01 ]. The ovarian weights in letrozole-treated group increased significantly[ (25.25 ± 9. 89) mg/100g vs ( 13. 10 ± 2. 70 ) mg/100g, P < 0. 01 ], arid the ovaries showed polycyst. The uterian weights in letrozole-treated group[ (41.46 ± 15.81 ) mg/100g vs (94. 81 ±18.00) mg/100g, P <0. 05 ] significantly decreased, and the endometriums presented atrophy. Conclusion Letrozole treated EM by means of increasing the apoptosis of the ectopic tissues. Letrozole would give ovarian over stimulating and the uterian weighting decreased as well as endometriums atrophy.

Objective To observe the protective effect of alcholic extractive of Cornu Cervi against the myocardial damage of acute myocardial infarction model rats. Methods The myocardial ischemia rats model was made by ligaturing their coronary attery, and the indexes including electrocardiogram, the area of acute myocardial infarction and ET of acute myocardial infarction model rats were observed after the acute period of myocardial damage. Results The alcoholic extractive of Cornu Cervi could obviously improve the degree of myocardial ischemia, reduce the area of myocardial ischemia, and lessen the level of ET. Conclusion The alcoholic extractive of Cornu Cervi could protect the myocardial against the damage of acute myocardial infarction model rats in some degree. The mechanism may be related to the reduction of value of ET of myocardial damage.

Objective:To investigate the clinical efficacy of point injection in treating lumbar intervertebral disc protrusion.Methods:One hundred and fifty-six patients with lumbar intervertebral disc protrusion were randomly allocated to an observation group of 80 cases (mecobalamin injection)and a control group of76 cases(vitamin B12 injection).Injection at Jiaji (Ex-B 2)points corresponding to the spinal segments of the pressed nerve roots was performed.Results:The efficacy rate Was 86.3% in the observation group and 78.9% in the control group.There was a statistically significant difference in curative effect between the two groups (P＜0.01).Conclusion:The results indicate that the clinical curative effect of acupoint injection of mecobalamin is significantly better than that of point injection of vitamin B12 in treating lumbar intervertebral disc protrusion.

Objective To discuss on the relationship among innovation,scientific nature and ethics in medical articles,in order to improve the academic quality of manuscripts.Methods Take Chinese Journal of Radiological Medicine and Protection as an example,innovation was emphasized in the field of radiation diagnosis and treatment,while the cases lack of innovation were pointed.However,the lessons from many papers showed that those papers only with innovation would be rejected because of defect in scientific nature and ethics.Results Innovation is the soul of the paper,and the scientific and ethical nature is the foundation and premise of innovation.Conclusions While the innovation is emphasized,the scientific nature and ethics of the thesis should be not ignored.These three aspects are integral.

Objective To construct the eukaryotic plasmid expression vector mediated short hairpin RNA(shRNA) interference targeting TLR4 gene,and transfect it into pancreatic adenocarcinoma cell line PANC1,then screen stably transfected clonal cell line.Methods Three shRNA interference expression plasmid vectors targeting the TLR4 gene were constructed,named TLR4-1,TLR4-2,TLR4-3.The shRNA plasmid with highest inhibitory efficiency was selected and transfected into PANC1 cells with liposome.The silencing efficiency and transfection efficiency of TLR4-shRNA was assayed with real-time quantitative PCR and flow cytometry analysis.Monoclonal cell with stable transfection of TLR4-shRNA were selected by geneticin 418 (C418) and limiting dilution analysis.Results Transient transfection efficiency of PANC1 was (46.72 ±5.06) ％.TLR4 mRNA expressions were 0.025 ± 0.004,0.027 ± 0.003,0.019 ± 0.006in cells transfected with TLR4-1,TLR4-2,TLR4-3,respectively,which were significantly lower than that in untransfected group (0.061 ±0.018) and negative control group (0.057 ±0.015,P ＜0.05).The transfection efficiency of TLR4-3 vector in stably transfected clones [(82.79 ±8.16)％] was significantly higher than that of transient transfection (P =0.001 ).The expression of TLR4 mRNA was decreased to 0.010 ± 0.002,which was significantly lower than that of transient transfection ( P =0.001 ).The expression of TLR4 protein was (0.54±0.32) ％,which was significantly lower than that of untransfected cells [( 87.42 ± 5.00 ) ％] and that of negative control [(82.9±5.00)％,P =0.000].Conclusions Stable transfection PANC1 cell lines with TLR4 gene silencing are successfully identified.