[Objective]To discussthe clinic effective and the application value of the fibula fixation in treating the complex and comminuted fracture of the tibiofibular.[Method]At first,we reduced and fixed up the fibula fracture anatomically as the support to reduce the tibia fracture indirectly,then we fixed up the tibia fracture by using intramedullary nail or external fixators.[Result]In the 23 cases of the tibiofibular fracture,10 cases of the fibula fracture were almost anatomically reduced,13 cases were reduced functionally,the wounds were cured without parenchyma infection or bone infection.The follow-up time was 13 months averagedly.The time of clinical healing was from 13 weeks to 21 weeks.The cases which were excellently cured was 20,which were well cured was 2,which was tolerably cured was 1 by the Johner-Wrushs grade standard.[Conclusion]This method was designed reasonably,operated simply,acceded with the minimal-invasive and biological rule of the bone fractuer treatment,profited to the heal of the fracture,it was a prefect mean to cure the complex and comminuted fracture of the tibiofibular.

Objective To screen and identify proteins that interact with receptor for advanced glycation end products (RAGE) with T7 select phage display system. Method GST-tagged fusion protein of RAGE was coated on glutathione suporflow resin and binding screens were performed with human lung T7 phage library. Results After 6 rounds of biopanning, 64 independent plaques were scraped and processed with EDTA. The inserted fragments in these plaques were amplified by PCR and the PCR products were purified by a gel recovery method. The sequences of the insertions were identified and analyzed with BLAST program in GeneBank. Twenty-five clones of encoding proteins were found. Conclusions T7 select phage display system is a convenient, rapid and effective method for screening binding proteins. This study provides an approach to explore complicated functions and novel mechanisms of RAGE-related signal transduction pathways.

[Objective]To explore the curative effect of the three diamen sional external fixation frame and limited internal fixation in treating the comminuted fracture of the shaft of humerus.[Method]Twenty-eight patients of the comminuted fracture of the shaft of humerus were treated from Jan 2000 to May 2006.According to the AO/ASIF,there were 5 cases of the B1 type,3 cases of the B2 type,9 cases of the B3 type,6 cases of the C1 type,4 cases of the C2 type,1 case of the C3 type.All of them were treated by the three diamensional external fixation frame and limited internal fixation of the screws or wires,and all of them were given the reasonable rehabilitation training at 3 day after the operation.The curative effect of the operation,the complications,the fracture healing and the function appraisal were analyzed.[Result]The 28 patients were followed-up for 10～15 months(average 13 months),27 cases had the frature healed and the healing rate was 96.4%.The healing period of the fracture lasted from 12 weeks to 33 weeks,average 19.5 weeks.Only 1 case was not healed.The functions of the shoulder and the elbow were normal,the excellent rate was 92.8%.[Conclusion]The three diamensional external fixation frame and limited internal fixation is a better method in treating the comminuted fracture of the shaft of humerus.It fits the rule of minimal-invasive-biology. The operation is simple,and the fixation is reliable,the function is satisfactory.

Objective To know the situation of upper GI diseases among the residents in Dong tai city. Methods With three levels examination methods of Occult Blood Bead Detector,Gastroscope and pathological examination,residents aged over 30 yrs in Dongtai were screened during Aug 2005 to Sept 2006. Results 70 809 residents,about 6.13% of whole city population,were taken the screen with occult blood bead detector. There were about 28 392 people positive; the positive rate was 40.10%. Among the positive people,there were 8 121 people taking gastroscope inspection,and while 2 294 of them also took the pathological examination. The gastroscope results showed that 4 636 people suffered from chronic superficial gastritis,1 431 rotten gastritis,565 esophagus gastritis,339 chronic & atrophic gastritis,24 suspicious cancers,53 stomach cancer,44 esophagus cancer. Of these 97 cases with cancers diagnosed by gastroscope,87 cases were confirmed by pathological examination,the false positive rate of gastroscope examination was about 10.31%; Among 2 197 of the non-cancer subjects diagnosed by gastroscope,there were 25 cases with cancer diagnosed by pathological method,the false negative rate of gastroscope was 1.14%. For those 112 cancer cases diagnosed by pathology,there were 83 cases (74.11%) who were in the early or middle phase of cancer. Conclusion The prevalence of upper GI diseases in Dongtai City was higher. It should strengthen the education and the improvement of the health situation.

OBJECTIVE To reduce the cross infection during gastroscopy and probe into the(administrative) measures in preventing infection.METHODS The hidden infection of sterilizing gastroscope was analyzed in order to work out the measures to prevent infection.RESULTS Choosing the correct methods of sterilizing gastroscope could(avoid the) incomplete sterilization and reduce the cross infection.CONCLUSIONS Be strict in sterilizing(procedure)s and protecting measures,and prevent the interactive infection in the hospital during gastroscopy.

Objective:To establish a determination method of Bergenin content in Dicha Kechuan oral liquid by HPLC.Methods:The separation was performed on SHIMADZU VP-ODS C18 column(150mm?4.6mm,5?m),the mobile phase was carbinol-water(20:80) with a flow rate of 1.0ml/min.The column temperature was room temperature and the detection wavelength was 275nm.Results:The linear range of Bergenin was 0.2575-5.150?g,the regression equation of working curve was Y=812702.2X-80626.3,r=0.9998,the average recovery was 101.4% and RSD 1.67%(n=5).Conclusion:This method is simple,reproducible,and can be used for quality control of Dicha Kechuan Oral Liquid and the semifinished product.

Objective To observe the therapeutic effect of abdominal acupuncture combined with Chinese medicine for the treatment of functional constipation. Methods Ninety-three qi-deficiency functional constipation patients were evenly randomized into western medicine group, Chinese medicine group and combination group. The western medicine group was given oral use of Dupbalac, Chinese medicine group was given modified Buzhong Yiqi decoction, and the combination group was given abdominal acupuncture and oral use of modified Buzhong Yiqi decoction. The treatment of the three groups covered 3 months. After treatment, the therapeutic effect was evaluated accord ing to the symptom scores and the examination results of gastrointestinal kinetics. Results ( 1) The total effective rate for relieving symptoms was 100.00% in combination group, 90.32% in Chinese medicine group, and 38.71% in western medicine group, and the effect was the best in the combination group ( P<0.05). ( 2) The combination group had stronger effect on relieving dyskinesis of anal sphincter, rectal sensibility, colonic transportation than Chinese medicine group and western medicine group (P<0.05). Conclusion Abdominal acupuncture combined with Chinese medicine shows certain therapeutic effect for the treatment of qi-deficiency functional constipation, and the effect is superior to that of Chinese medicine or Dupbalac.

Objective To evaluate the effect of isoflurane or sevoflurane inhalation before and after gestation on the offspring brain development. Methods Thirty female adult Sprague Dawley rats were randomly assigned into 5 groups(n=6 each):control group(group C),group that ex?posed to isoflurane with the concentration of 1.6%for 6 hours before gestation(group BI),group that exposed to isoflurane with the concentration of 1.6%at the 6th,10th,14th and 18th day for 6 hours(group PI),group that exposed to sevoflurane with the concentration of 2.4%for 6 hours before gestation(group BS),and group exposed to sevoflurane with the concentration of 2.4%at the 6th,10th,14th and 18th day for 6 hours after gestation (group PS). Twelve offspring rats from pregnant rats in each group were chosen on the day of birth(T1),and 7th,14th and 28th days after birth(T2, T3 and T4)and sacrificed,and the hippocampi were then isolated. Hematoxylin and eosin stain were adopted to observe the tissue pathological change. Electron microscope was used to observe the neuron ultrastructure change of hippocampus. Immolunohistochemistry was used to detect cas?pase?3,the expression of growth associated protein?43(GAP?43)and neuronal nitric oxide synthase(nNOS). Results Compared with group C, no significant change was found in caspase?3,GAP?43 and nNOS expression in offspring rat hippocampus in groups BI and BS(P>0.05),and no damage change in hippocampal was seen by HE staining and electron microscopy. In group PI and PB,the expression of caspase?3 was significantly up?regulated,the expression of GAP?43 and nNOS was down?regulated at T1 to T3(P<0.01),and structural changes in cell were seen by electron microscopy. In group PI,significant pathological changes in hippocampal were seen by HE staining at T1 to T3. Compared with group PI,the expres?sion of GAP?43 and nNOS was significantly up?regulated(P<0.01),and the expression of caspase?3 was down?regulated at T1 to T3(P<0.01). Conclusion Isoflurane or sevoflurane inhalation before gestation does not affect the offspring brain development,while isoflurane or sevoflurane in?halation after gestation can induce transient abnormal change of offspring brain development,and isoflurane′s toxicity was greater than sevoflurane.

Numbness is a common symptom in many diseases.In terms of cognition about numbness in past dynasties,and based on clinic observation and practice,the research of Guizhi Huangqi Wuwu Decoction is necessary.As a classic prescription,it is important for doctors to concern about combination of disease and syndrome,but also attaches importance to correspondence of prescription and syndrome.

The article emphasizes to expound the correlative characteristic theory of science of Tujia herbs from nation and district,concept and attribute of subject,medical foundament,basic theory(denomination feature,nature and class of herb),application(preparation,compatibility,contraindications,dosage,administration and pharmacotherapy) and so on,and analyzes the various objective existing differences between science of Tujia herbs and Chinese materia medica,reveals the mirror meaning between the two traditional materia medica systems,and defines the concept of "science of Tujia herbs" for the first time,then lets scholars recognize and understand the science of ethno-medicines of China more completely,and provids some study ideas for research on ethnic medicine.